Journal: Biochimica et biophysica acta

Article Title: Important roles for membrane lipids in haloarchaeal bioenergetics.

doi: 10.1016/j.bbamem.2016.08.010

Figure Lengend Snippet: Figure 2: Lipidomics of haloarchaea. Relative abundance of major lipid classes (glycerolipids, menaquinones, carotenoids and squalenes) in Hfx. volcanii, Hlb. salinarum, Hrr. lacusprofundi, Hrr. sodomense and Hpn. natans. The generation times for (*), (**) and (***) are reported in [57], [27] and [42], respectively. A more detailed contribution of only glycerolipids is given in (B), where for all five species, the anionic lipids (sum of PG, Me-PGP, SDG and CL) clearly dominated the total polar lipid fraction. Note that Hrr. sodomense and Hpn. natans were each analyzed only in a singlet analysis. Relative percentages in tabular form are provided in Table S2. Compound abbreviations are presented in Fig. S2; n.d., not determined.

Article Snippet: [°C] O2 [%] pH Hfx. volcanii (ATCC 29605) ATCC 974 Yes 2 250 37 21 7 Hrr. lacusprofundi (ATCC 49238) ATCC 974 Yes 2 250 37 21 7 Hbt. salinarum (DSM 617) [41] No 4 81 37 21 7 Hrr. sodomense (DSM 3755)* DSMZ 372 No [42] 3.4 81 37 21 7 Hpn. natans (DSM 17985)* DSMZ 1130 Yes [43] 3 100 37 21 7 (B) Hfx. volcanii only ATCC 974 lower ------------------------------------------------------- higher Na + [M] 1 2 4 5 Mg 2+ [mM] 50 (75) (150) 250 (500) 1000 Temp.

Techniques:

Journal: Biochimica et biophysica acta

Article Title: Important roles for membrane lipids in haloarchaeal bioenergetics.

doi: 10.1016/j.bbamem.2016.08.010

Figure Lengend Snippet: Figure 8: Cell size and morphology of Hfx. volcanii after a rapid five-fold decrease of Mg2+ ions. (A) Micrographs showing a time series (two hours, two days and six days) of Hfx. volcanii reacting to Mg2+ concentrations, when transferred from a culture grown under standard conditions at 250 mM Mg2+ (late stationary phase) into a fresh, sterile media containing 50 mM Mg2+. Scale bars represent a length of 10µm. In (B) we illustrate the average cell shape of Hfx. volcanii for each phase. Membrane regions with highest curvature stress are highlighted in red. Ø indicates the averaged diameter of cells (n=20).

Article Snippet: [°C] O2 [%] pH Hfx. volcanii (ATCC 29605) ATCC 974 Yes 2 250 37 21 7 Hrr. lacusprofundi (ATCC 49238) ATCC 974 Yes 2 250 37 21 7 Hbt. salinarum (DSM 617) [41] No 4 81 37 21 7 Hrr. sodomense (DSM 3755)* DSMZ 372 No [42] 3.4 81 37 21 7 Hpn. natans (DSM 17985)* DSMZ 1130 Yes [43] 3 100 37 21 7 (B) Hfx. volcanii only ATCC 974 lower ------------------------------------------------------- higher Na + [M] 1 2 4 5 Mg 2+ [mM] 50 (75) (150) 250 (500) 1000 Temp.

Techniques: Sterility, Membrane

Journal: Biochimica et biophysica acta

Article Title: Important roles for membrane lipids in haloarchaeal bioenergetics.

doi: 10.1016/j.bbamem.2016.08.010

Figure Lengend Snippet: Figure 9: Potential interactions of Mg2+ ions with different negatively charged lipid headgroups. We suggest that a single divalent cation (here Mg2+) may form a CL-like structure (or CL surrogate) by fusing two single anionic lipids such as PG. Hence, under insufficient Mg2+ levels, Hfx. volcanii is forced to upregulate their CL levels counteracting the absence of CL-surrogates. At elevated Mg2+ levels, we observed an upregulation of Me-PGP moieties, potentially carrying two negative charges, possibly functioning as a scavenger for divalent cations. Hence, Me-PGP may enable haloarchaea to act as buffer for elevated Mg2+ levels by modulating the extent of the binding between two PG molecules. We here propose that the lipids CL and Me-PGP may be used to control permeability properties and avoid continuous polymerization of membrane surfaces, respectively (see text).

Article Snippet: [°C] O2 [%] pH Hfx. volcanii (ATCC 29605) ATCC 974 Yes 2 250 37 21 7 Hrr. lacusprofundi (ATCC 49238) ATCC 974 Yes 2 250 37 21 7 Hbt. salinarum (DSM 617) [41] No 4 81 37 21 7 Hrr. sodomense (DSM 3755)* DSMZ 372 No [42] 3.4 81 37 21 7 Hpn. natans (DSM 17985)* DSMZ 1130 Yes [43] 3 100 37 21 7 (B) Hfx. volcanii only ATCC 974 lower ------------------------------------------------------- higher Na + [M] 1 2 4 5 Mg 2+ [mM] 50 (75) (150) 250 (500) 1000 Temp.

Techniques: Binding Assay, Control, Permeability, Membrane

Journal: Biochimica et biophysica acta

Article Title: Important roles for membrane lipids in haloarchaeal bioenergetics.

doi: 10.1016/j.bbamem.2016.08.010

Figure Lengend Snippet: Figure 11: Suggested benefits of having a pleomorphic rather than rod-shaped lifestyle. Cartoon (A) shows how Hfx. volcanii may use their pleomorphic cell behavior for purposeful locomotion as well as creating turbulences in close vicinity of the membrane and thus enhancing diffusional transport. Note that the here illustrated pleomorphic cycle has been performed, at ideal growth conditions, at a rate faster than once per second. A video demonstrating the pleomorphic lifestyle of Hfx. volcanii is provided in Supplementary Movie 1. In (B) we hypothesize how shape-shifting morphologies may stimulate internal membrane motion which in turn can enhance energy production (e.g., accelerating the motion of the electron carriers MKs).

Article Snippet: [°C] O2 [%] pH Hfx. volcanii (ATCC 29605) ATCC 974 Yes 2 250 37 21 7 Hrr. lacusprofundi (ATCC 49238) ATCC 974 Yes 2 250 37 21 7 Hbt. salinarum (DSM 617) [41] No 4 81 37 21 7 Hrr. sodomense (DSM 3755)* DSMZ 372 No [42] 3.4 81 37 21 7 Hpn. natans (DSM 17985)* DSMZ 1130 Yes [43] 3 100 37 21 7 (B) Hfx. volcanii only ATCC 974 lower ------------------------------------------------------- higher Na + [M] 1 2 4 5 Mg 2+ [mM] 50 (75) (150) 250 (500) 1000 Temp.

Techniques: Membrane

Journal: Biogeosciences

Article Title: A laboratory experiment of intact polar lipid degradation in sandy sediments

doi: 10.5194/bg-8-2547-2011

Figure Lengend Snippet: Fig. 1. Chemical structures of IPLs characteristic for the two organisms used in this study: (A): diacylglycerol ester-bound core lipids (DAGs) of Saccharomyces cerevisiae bound to: phosphatidylethanolamine (PE), phosphatidylcholine (PC), phosphatidylinositol (PI) and phosphatidylserine (PS); (B): diphytanylglycerol ether-bound core lipids (DPGs) of Haloferax volcanii bound to: sulfono diglyco dialkyl- glycerol S-GL-1 (nomenclature according to Sprott et al., 2003), phosphatidylglycerol (PG) and phosphatidylglycerol methylphosphate (PGP-Me).

Article Snippet: Etherderived IPLs (diphytanylglycerols = DPGs) were originated from pure culture of Haloferax volcanii (DSM No. 16227) grown at 37 ◦C in “Haloferax sulfurifontis medium” (DSMZ No. 1018).

Techniques:

Journal: Biogeosciences

Article Title: A laboratory experiment of intact polar lipid degradation in sandy sediments

doi: 10.5194/bg-8-2547-2011

Figure Lengend Snippet: Fig. 3. HPLC-ESI-MS base-peak intensity (BPI) chromatograms in negative ion-mode of (A): total extract of Saccharomyces cere- visiae; (B): total extract of Haloferax volcanii; (C): total extract of the untreated control at day 0; (D): total extract of the degradation experiment, sample taken at day 0; (E): total extract of the degra- dation experiment, sample taken at day 97. For abbreviations see Fig. 1. Please note that the observed peak intensities cannot be di- rectly transferred into concentrations.

Article Snippet: Etherderived IPLs (diphytanylglycerols = DPGs) were originated from pure culture of Haloferax volcanii (DSM No. 16227) grown at 37 ◦C in “Haloferax sulfurifontis medium” (DSMZ No. 1018).

Techniques: Control

Journal: Methods in molecular biology (Clifton, N.J.)

Article Title: Application of Archaea in Deubiquitinase-Like Enzyme Discovery and Activity Assay

doi: 10.1007/978-1-0716-2803-4_10

Figure Lengend Snippet: Example of PCR conditions used for isolation of an archaeal DUB gene ( hvo_2505 , HvJAMM1)

Article Snippet: 2 Haloferax volcanii DS2 genomic DNA ordered from ATCC (ATCC29605) or prepared from whole cells by osmotic lysis (see Note 1).

Techniques: Isolation

Journal: Methods in molecular biology (Clifton, N.J.)

Article Title: Application of Archaea in Deubiquitinase-Like Enzyme Discovery and Activity Assay

doi: 10.1007/978-1-0716-2803-4_10

Figure Lengend Snippet: Example of the setup for analyzing DUB/DUB-like activity using Ubl-modified proteins produced in H. volcanii as a substrate

Article Snippet: 2 Haloferax volcanii DS2 genomic DNA ordered from ATCC (ATCC29605) or prepared from whole cells by osmotic lysis (see Note 1).

Techniques: Activity Assay, Produced

Journal: Frontiers in Microbiology

Article Title: MinD2 modulates cell shape and motility in the archaeon Haloferax volcanii

doi: 10.3389/fmicb.2024.1474570

Figure Lengend Snippet: Proposed model for MinD2 function in Haloferax volcanii. H. volcanii cells show growth-dependent cell shape morphology transitioning from rods to plate-shaped cells. We show that MinD2 has a strong effect on shape, with ∆minD2 mutant cells predominantly adopting a plate shape. We propose that the shape phenotype impacts the proper positioning of the archaellum (archaellum motor: yellow square and ArlD: orange ovals) and chemotactic machinery (dark green lines). According to this model, in ∆minD2 cells, the localization of both machineries is diffused (light green) with a very few cells showing the ability to localize the chemosensory arrays in early log phase. As the growth stage progresses, only chemosensory arrays remain diffused (dark green) and the archaellum motor is able to localize at a pole. Previous studies on MinD4 ( ; ), shows the ∆minD4 mutant cells has an effect on the positioning on archaellum and chemosensory arrays. Together, we postulate that deleting both MinD homologues ( ∆minD2minD4 ) has a synergistic effect, resulting in these cells being unable to localize both archaellum and chemosensory arrays to the pole at any stage of the growth phase.

Article Snippet: To ensure the removal of ribosomal RNA, 2 μg of input RNA was treated with a Ribopool designed specifically for Haloferax volcanii (siTOOLs) according to the manufacturer’s instructions.

Techniques: Mutagenesis