hacat Search Results


keratinocytes  (CLS Cell Lines Service GmbH)
98
CLS Cell Lines Service GmbH keratinocytes
Dose-dependent viability profile of HaCaT <t>keratinocytes</t> exposed to optimized oleogels formulations (SFW, RBW, HSA) over 24 and 48 h. Data are expressed as mean ± SD (n = 3). Different letters: significant difference between treatments ( p < 0.05). Concentration (μg/mL): essential oil blend equivalent.
Keratinocytes, supplied by CLS Cell Lines Service GmbH, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hacat/pmc13025447-696-5-9?v=CLS+Cell+Lines+Service+GmbH
Average 98 stars, based on 1 article reviews
keratinocytes - by Bioz Stars, 2026-08
98/100 stars
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hacat  (AcceGen Biotechnology)
93
AcceGen Biotechnology hacat
Dose-dependent viability profile of HaCaT <t>keratinocytes</t> exposed to optimized oleogels formulations (SFW, RBW, HSA) over 24 and 48 h. Data are expressed as mean ± SD (n = 3). Different letters: significant difference between treatments ( p < 0.05). Concentration (μg/mL): essential oil blend equivalent.
Hacat, supplied by AcceGen Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hacat/10__1158_slash_0008___5472__can___20___0259-49-19-20?v=AcceGen+Biotechnology
Average 93 stars, based on 1 article reviews
hacat - by Bioz Stars, 2026-08
93/100 stars
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hacat cells  (Hormel Health Labs)
90
Hormel Health Labs hacat cells
(A) Possible effect of caffeic acid in Scrophulariae Radix and Malvae Semen. (B) <t>HaCaT</t> <t>cells</t> were pretreated with caffeic acid (50 and 100 μM) for 1 hr, and then cells were exposed to TPA (100 nM) for additional 8 hr. (C) Cells were treated with TPA (100 nM) in the presence of caffeic acid (50 and 100 μM) for 2 hr. The NF-κB DNA binding activity was assessed by the gel-shift assay. The nuclear extracts were prepared and incubated with the radiolabeled oligonucleotides containing κB consensus sequence for the analysis of NF-κB DNA binding by EMSA. (D) Nuclear proteins were separated by 10% SDS-polyacrylamide gel electrophoresis and immunoblotted with p65 antibody. Lamin B was used as markers of nuclear proteins. (E) The cytosolic extracts prepared from cells incubated with TPA for 3 hr in the presence or absence of caffeic acid were immunoblotted with was analyzed by Western blotting to examine the expression of IκBα. (F) HaCaT cells were treated with TNF-α (20 nM) in the absence or presence of caffeic acid (100 μM) for 24 hr and then the isolated RNA was reverse-transcribed and amplified as described in Materials and Methods. Expression of il-8 and gapdh mRNA was measured by RT-PCR.
Hacat Cells, supplied by Hormel Health Labs, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hacat/pmc04640719-144-0-9?v=Hormel+Health+Labs
Average 90 stars, based on 1 article reviews
hacat cells - by Bioz Stars, 2026-08
90/100 stars
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c6-loaded nps hacat cell  (China Center for Type Culture Collection)
90
China Center for Type Culture Collection c6-loaded nps hacat cell
(A) Possible effect of caffeic acid in Scrophulariae Radix and Malvae Semen. (B) <t>HaCaT</t> <t>cells</t> were pretreated with caffeic acid (50 and 100 μM) for 1 hr, and then cells were exposed to TPA (100 nM) for additional 8 hr. (C) Cells were treated with TPA (100 nM) in the presence of caffeic acid (50 and 100 μM) for 2 hr. The NF-κB DNA binding activity was assessed by the gel-shift assay. The nuclear extracts were prepared and incubated with the radiolabeled oligonucleotides containing κB consensus sequence for the analysis of NF-κB DNA binding by EMSA. (D) Nuclear proteins were separated by 10% SDS-polyacrylamide gel electrophoresis and immunoblotted with p65 antibody. Lamin B was used as markers of nuclear proteins. (E) The cytosolic extracts prepared from cells incubated with TPA for 3 hr in the presence or absence of caffeic acid were immunoblotted with was analyzed by Western blotting to examine the expression of IκBα. (F) HaCaT cells were treated with TNF-α (20 nM) in the absence or presence of caffeic acid (100 μM) for 24 hr and then the isolated RNA was reverse-transcribed and amplified as described in Materials and Methods. Expression of il-8 and gapdh mRNA was measured by RT-PCR.
C6 Loaded Nps Hacat Cell, supplied by China Center for Type Culture Collection, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hacat/10__2147_slash_ijn__s108545-127-34-40?v=China+Center+for+Type+Culture+Collection
Average 90 stars, based on 1 article reviews
c6-loaded nps hacat cell - by Bioz Stars, 2026-08
90/100 stars
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human immortalized keratinocyte cell line hacat  (AddexBio Inc)
90
AddexBio Inc human immortalized keratinocyte cell line hacat
(A) Possible effect of caffeic acid in Scrophulariae Radix and Malvae Semen. (B) <t>HaCaT</t> <t>cells</t> were pretreated with caffeic acid (50 and 100 μM) for 1 hr, and then cells were exposed to TPA (100 nM) for additional 8 hr. (C) Cells were treated with TPA (100 nM) in the presence of caffeic acid (50 and 100 μM) for 2 hr. The NF-κB DNA binding activity was assessed by the gel-shift assay. The nuclear extracts were prepared and incubated with the radiolabeled oligonucleotides containing κB consensus sequence for the analysis of NF-κB DNA binding by EMSA. (D) Nuclear proteins were separated by 10% SDS-polyacrylamide gel electrophoresis and immunoblotted with p65 antibody. Lamin B was used as markers of nuclear proteins. (E) The cytosolic extracts prepared from cells incubated with TPA for 3 hr in the presence or absence of caffeic acid were immunoblotted with was analyzed by Western blotting to examine the expression of IκBα. (F) HaCaT cells were treated with TNF-α (20 nM) in the absence or presence of caffeic acid (100 μM) for 24 hr and then the isolated RNA was reverse-transcribed and amplified as described in Materials and Methods. Expression of il-8 and gapdh mRNA was measured by RT-PCR.
Human Immortalized Keratinocyte Cell Line Hacat, supplied by AddexBio Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hacat/pmc07459541-84-13-15?v=AddexBio+Inc
Average 90 stars, based on 1 article reviews
human immortalized keratinocyte cell line hacat - by Bioz Stars, 2026-08
90/100 stars
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hacat  (BioResource International Inc)
90
BioResource International Inc hacat
(A) Possible effect of caffeic acid in Scrophulariae Radix and Malvae Semen. (B) <t>HaCaT</t> <t>cells</t> were pretreated with caffeic acid (50 and 100 μM) for 1 hr, and then cells were exposed to TPA (100 nM) for additional 8 hr. (C) Cells were treated with TPA (100 nM) in the presence of caffeic acid (50 and 100 μM) for 2 hr. The NF-κB DNA binding activity was assessed by the gel-shift assay. The nuclear extracts were prepared and incubated with the radiolabeled oligonucleotides containing κB consensus sequence for the analysis of NF-κB DNA binding by EMSA. (D) Nuclear proteins were separated by 10% SDS-polyacrylamide gel electrophoresis and immunoblotted with p65 antibody. Lamin B was used as markers of nuclear proteins. (E) The cytosolic extracts prepared from cells incubated with TPA for 3 hr in the presence or absence of caffeic acid were immunoblotted with was analyzed by Western blotting to examine the expression of IκBα. (F) HaCaT cells were treated with TNF-α (20 nM) in the absence or presence of caffeic acid (100 μM) for 24 hr and then the isolated RNA was reverse-transcribed and amplified as described in Materials and Methods. Expression of il-8 and gapdh mRNA was measured by RT-PCR.
Hacat, supplied by BioResource International Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hacat/pm34848458-59-7-18?v=BioResource+International+Inc
Average 90 stars, based on 1 article reviews
hacat - by Bioz Stars, 2026-08
90/100 stars
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human keratinocytes hacat cells  (AddexBio Inc)
90
AddexBio Inc human keratinocytes hacat cells
(A) Possible effect of caffeic acid in Scrophulariae Radix and Malvae Semen. (B) <t>HaCaT</t> <t>cells</t> were pretreated with caffeic acid (50 and 100 μM) for 1 hr, and then cells were exposed to TPA (100 nM) for additional 8 hr. (C) Cells were treated with TPA (100 nM) in the presence of caffeic acid (50 and 100 μM) for 2 hr. The NF-κB DNA binding activity was assessed by the gel-shift assay. The nuclear extracts were prepared and incubated with the radiolabeled oligonucleotides containing κB consensus sequence for the analysis of NF-κB DNA binding by EMSA. (D) Nuclear proteins were separated by 10% SDS-polyacrylamide gel electrophoresis and immunoblotted with p65 antibody. Lamin B was used as markers of nuclear proteins. (E) The cytosolic extracts prepared from cells incubated with TPA for 3 hr in the presence or absence of caffeic acid were immunoblotted with was analyzed by Western blotting to examine the expression of IκBα. (F) HaCaT cells were treated with TNF-α (20 nM) in the absence or presence of caffeic acid (100 μM) for 24 hr and then the isolated RNA was reverse-transcribed and amplified as described in Materials and Methods. Expression of il-8 and gapdh mRNA was measured by RT-PCR.
Human Keratinocytes Hacat Cells, supplied by AddexBio Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hacat/pm31326624-107-0-9?v=AddexBio+Inc
Average 90 stars, based on 1 article reviews
human keratinocytes hacat cells - by Bioz Stars, 2026-08
90/100 stars
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keratinocytes hacat  (AddexBio Inc)
90
AddexBio Inc keratinocytes hacat
(A) Possible effect of caffeic acid in Scrophulariae Radix and Malvae Semen. (B) <t>HaCaT</t> <t>cells</t> were pretreated with caffeic acid (50 and 100 μM) for 1 hr, and then cells were exposed to TPA (100 nM) for additional 8 hr. (C) Cells were treated with TPA (100 nM) in the presence of caffeic acid (50 and 100 μM) for 2 hr. The NF-κB DNA binding activity was assessed by the gel-shift assay. The nuclear extracts were prepared and incubated with the radiolabeled oligonucleotides containing κB consensus sequence for the analysis of NF-κB DNA binding by EMSA. (D) Nuclear proteins were separated by 10% SDS-polyacrylamide gel electrophoresis and immunoblotted with p65 antibody. Lamin B was used as markers of nuclear proteins. (E) The cytosolic extracts prepared from cells incubated with TPA for 3 hr in the presence or absence of caffeic acid were immunoblotted with was analyzed by Western blotting to examine the expression of IκBα. (F) HaCaT cells were treated with TNF-α (20 nM) in the absence or presence of caffeic acid (100 μM) for 24 hr and then the isolated RNA was reverse-transcribed and amplified as described in Materials and Methods. Expression of il-8 and gapdh mRNA was measured by RT-PCR.
Keratinocytes Hacat, supplied by AddexBio Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hacat/pm36979541-35-0-3?v=AddexBio+Inc
Average 90 stars, based on 1 article reviews
keratinocytes hacat - by Bioz Stars, 2026-08
90/100 stars
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human immortalized epidermal cell line hacat  (China Center for Type Culture Collection)
90
China Center for Type Culture Collection human immortalized epidermal cell line hacat
(A) Possible effect of caffeic acid in Scrophulariae Radix and Malvae Semen. (B) <t>HaCaT</t> <t>cells</t> were pretreated with caffeic acid (50 and 100 μM) for 1 hr, and then cells were exposed to TPA (100 nM) for additional 8 hr. (C) Cells were treated with TPA (100 nM) in the presence of caffeic acid (50 and 100 μM) for 2 hr. The NF-κB DNA binding activity was assessed by the gel-shift assay. The nuclear extracts were prepared and incubated with the radiolabeled oligonucleotides containing κB consensus sequence for the analysis of NF-κB DNA binding by EMSA. (D) Nuclear proteins were separated by 10% SDS-polyacrylamide gel electrophoresis and immunoblotted with p65 antibody. Lamin B was used as markers of nuclear proteins. (E) The cytosolic extracts prepared from cells incubated with TPA for 3 hr in the presence or absence of caffeic acid were immunoblotted with was analyzed by Western blotting to examine the expression of IκBα. (F) HaCaT cells were treated with TNF-α (20 nM) in the absence or presence of caffeic acid (100 μM) for 24 hr and then the isolated RNA was reverse-transcribed and amplified as described in Materials and Methods. Expression of il-8 and gapdh mRNA was measured by RT-PCR.
Human Immortalized Epidermal Cell Line Hacat, supplied by China Center for Type Culture Collection, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hacat/10__1016_slash_j__mtbio__2024__101212-85-4-11?v=China+Center+for+Type+Culture+Collection
Average 90 stars, based on 1 article reviews
human immortalized epidermal cell line hacat - by Bioz Stars, 2026-08
90/100 stars
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hacat cells  (Cosmo Bio USA)
90
Cosmo Bio USA hacat cells
(A) Possible effect of caffeic acid in Scrophulariae Radix and Malvae Semen. (B) <t>HaCaT</t> <t>cells</t> were pretreated with caffeic acid (50 and 100 μM) for 1 hr, and then cells were exposed to TPA (100 nM) for additional 8 hr. (C) Cells were treated with TPA (100 nM) in the presence of caffeic acid (50 and 100 μM) for 2 hr. The NF-κB DNA binding activity was assessed by the gel-shift assay. The nuclear extracts were prepared and incubated with the radiolabeled oligonucleotides containing κB consensus sequence for the analysis of NF-κB DNA binding by EMSA. (D) Nuclear proteins were separated by 10% SDS-polyacrylamide gel electrophoresis and immunoblotted with p65 antibody. Lamin B was used as markers of nuclear proteins. (E) The cytosolic extracts prepared from cells incubated with TPA for 3 hr in the presence or absence of caffeic acid were immunoblotted with was analyzed by Western blotting to examine the expression of IκBα. (F) HaCaT cells were treated with TNF-α (20 nM) in the absence or presence of caffeic acid (100 μM) for 24 hr and then the isolated RNA was reverse-transcribed and amplified as described in Materials and Methods. Expression of il-8 and gapdh mRNA was measured by RT-PCR.
Hacat Cells, supplied by Cosmo Bio USA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hacat/pm34351912-169-0-5?v=Cosmo+Bio+USA
Average 90 stars, based on 1 article reviews
hacat cells - by Bioz Stars, 2026-08
90/100 stars
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hacat cell line c5  (China Center for Type Culture Collection)
90
China Center for Type Culture Collection hacat cell line c5
(A) Possible effect of caffeic acid in Scrophulariae Radix and Malvae Semen. (B) <t>HaCaT</t> <t>cells</t> were pretreated with caffeic acid (50 and 100 μM) for 1 hr, and then cells were exposed to TPA (100 nM) for additional 8 hr. (C) Cells were treated with TPA (100 nM) in the presence of caffeic acid (50 and 100 μM) for 2 hr. The NF-κB DNA binding activity was assessed by the gel-shift assay. The nuclear extracts were prepared and incubated with the radiolabeled oligonucleotides containing κB consensus sequence for the analysis of NF-κB DNA binding by EMSA. (D) Nuclear proteins were separated by 10% SDS-polyacrylamide gel electrophoresis and immunoblotted with p65 antibody. Lamin B was used as markers of nuclear proteins. (E) The cytosolic extracts prepared from cells incubated with TPA for 3 hr in the presence or absence of caffeic acid were immunoblotted with was analyzed by Western blotting to examine the expression of IκBα. (F) HaCaT cells were treated with TNF-α (20 nM) in the absence or presence of caffeic acid (100 μM) for 24 hr and then the isolated RNA was reverse-transcribed and amplified as described in Materials and Methods. Expression of il-8 and gapdh mRNA was measured by RT-PCR.
Hacat Cell Line C5, supplied by China Center for Type Culture Collection, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hacat/pm38054565-33-6-17?v=China+Center+for+Type+Culture+Collection
Average 90 stars, based on 1 article reviews
hacat cell line c5 - by Bioz Stars, 2026-08
90/100 stars
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hacat (human keratinocytes) cells  (National Centre for Cell Science)
90
National Centre for Cell Science hacat (human keratinocytes) cells
(A) Possible effect of caffeic acid in Scrophulariae Radix and Malvae Semen. (B) <t>HaCaT</t> <t>cells</t> were pretreated with caffeic acid (50 and 100 μM) for 1 hr, and then cells were exposed to TPA (100 nM) for additional 8 hr. (C) Cells were treated with TPA (100 nM) in the presence of caffeic acid (50 and 100 μM) for 2 hr. The NF-κB DNA binding activity was assessed by the gel-shift assay. The nuclear extracts were prepared and incubated with the radiolabeled oligonucleotides containing κB consensus sequence for the analysis of NF-κB DNA binding by EMSA. (D) Nuclear proteins were separated by 10% SDS-polyacrylamide gel electrophoresis and immunoblotted with p65 antibody. Lamin B was used as markers of nuclear proteins. (E) The cytosolic extracts prepared from cells incubated with TPA for 3 hr in the presence or absence of caffeic acid were immunoblotted with was analyzed by Western blotting to examine the expression of IκBα. (F) HaCaT cells were treated with TNF-α (20 nM) in the absence or presence of caffeic acid (100 μM) for 24 hr and then the isolated RNA was reverse-transcribed and amplified as described in Materials and Methods. Expression of il-8 and gapdh mRNA was measured by RT-PCR.
Hacat (Human Keratinocytes) Cells, supplied by National Centre for Cell Science, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hacat/pm28155509-50-3-12?v=National+Centre+for+Cell+Science
Average 90 stars, based on 1 article reviews
hacat (human keratinocytes) cells - by Bioz Stars, 2026-08
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Image Search Results


Dose-dependent viability profile of HaCaT keratinocytes exposed to optimized oleogels formulations (SFW, RBW, HSA) over 24 and 48 h. Data are expressed as mean ± SD (n = 3). Different letters: significant difference between treatments ( p < 0.05). Concentration (μg/mL): essential oil blend equivalent.

Journal: Gels

Article Title: Oleogel Dressings for Skin Therapy: Physicochemical and Bioactive Properties of Cosmetic Oil-Based Systems Enriched with Essential Oils

doi: 10.3390/gels12030248

Figure Lengend Snippet: Dose-dependent viability profile of HaCaT keratinocytes exposed to optimized oleogels formulations (SFW, RBW, HSA) over 24 and 48 h. Data are expressed as mean ± SD (n = 3). Different letters: significant difference between treatments ( p < 0.05). Concentration (μg/mL): essential oil blend equivalent.

Article Snippet: The viability of immortalized human keratinocytes (HaCaT, CLS 300493; Cell Lines Service GmbH, Germany) was assessed.

Techniques: Concentration Assay

(A) Possible effect of caffeic acid in Scrophulariae Radix and Malvae Semen. (B) HaCaT cells were pretreated with caffeic acid (50 and 100 μM) for 1 hr, and then cells were exposed to TPA (100 nM) for additional 8 hr. (C) Cells were treated with TPA (100 nM) in the presence of caffeic acid (50 and 100 μM) for 2 hr. The NF-κB DNA binding activity was assessed by the gel-shift assay. The nuclear extracts were prepared and incubated with the radiolabeled oligonucleotides containing κB consensus sequence for the analysis of NF-κB DNA binding by EMSA. (D) Nuclear proteins were separated by 10% SDS-polyacrylamide gel electrophoresis and immunoblotted with p65 antibody. Lamin B was used as markers of nuclear proteins. (E) The cytosolic extracts prepared from cells incubated with TPA for 3 hr in the presence or absence of caffeic acid were immunoblotted with was analyzed by Western blotting to examine the expression of IκBα. (F) HaCaT cells were treated with TNF-α (20 nM) in the absence or presence of caffeic acid (100 μM) for 24 hr and then the isolated RNA was reverse-transcribed and amplified as described in Materials and Methods. Expression of il-8 and gapdh mRNA was measured by RT-PCR.

Journal: PLoS ONE

Article Title: PharmDB-K: Integrated Bio-Pharmacological Network Database for Traditional Korean Medicine

doi: 10.1371/journal.pone.0142624

Figure Lengend Snippet: (A) Possible effect of caffeic acid in Scrophulariae Radix and Malvae Semen. (B) HaCaT cells were pretreated with caffeic acid (50 and 100 μM) for 1 hr, and then cells were exposed to TPA (100 nM) for additional 8 hr. (C) Cells were treated with TPA (100 nM) in the presence of caffeic acid (50 and 100 μM) for 2 hr. The NF-κB DNA binding activity was assessed by the gel-shift assay. The nuclear extracts were prepared and incubated with the radiolabeled oligonucleotides containing κB consensus sequence for the analysis of NF-κB DNA binding by EMSA. (D) Nuclear proteins were separated by 10% SDS-polyacrylamide gel electrophoresis and immunoblotted with p65 antibody. Lamin B was used as markers of nuclear proteins. (E) The cytosolic extracts prepared from cells incubated with TPA for 3 hr in the presence or absence of caffeic acid were immunoblotted with was analyzed by Western blotting to examine the expression of IκBα. (F) HaCaT cells were treated with TNF-α (20 nM) in the absence or presence of caffeic acid (100 μM) for 24 hr and then the isolated RNA was reverse-transcribed and amplified as described in Materials and Methods. Expression of il-8 and gapdh mRNA was measured by RT-PCR.

Article Snippet: HaCaT cells were kindly gifted from Dr. Zigang Dong (Hormel Institute, University of Minnesota, MN, USA) and were maintained routinely in DMEM medium supplemented with 10% fetal bovine serum and a 100 ng/ml penicillin/streptomycin/fungizone mixture at 37°C in a humidified atmosphere of 5% CO2/95% air.

Techniques: Binding Assay, Activity Assay, Gel Shift, Incubation, Sequencing, Polyacrylamide Gel Electrophoresis, Western Blot, Expressing, Isolation, Reverse Transcription, Amplification, Reverse Transcription Polymerase Chain Reaction