h6pd Search Results


gene exp h6pd hs00188728 m1  (Thermo Fisher)
89
Thermo Fisher gene exp h6pd hs00188728 m1
Bivariate correlations between expression of Vitamin D Receptor ( VDR ) and other skeletal muscle target genes. Data are Spearman correlation coefficients (rho)), with 95% confidence intervals (CI) and p values in brackets).
Gene Exp H6pd Hs00188728 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 89/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti h6pd antibody  (OriGene)
90
OriGene anti h6pd antibody
Fig. 1. <t>H6PD</t> and regulation of glucocorticoid metabolism with enz resistance. (A) Effects of enz treatment on H6PD, GR, and 11-HSD2 in the LAPC4 CRPC model as assessed by immunoblot. (B) NADPH generation in LAPC4 cells with enz treatment. NADPH was assessed using a bioluminescence assay in ER extracted from control and enz-treated cells. (C) H6PD and 11-HSD2 protein in tissues from enz-treated patients with prostate cancer. Bar graphs represent band intensity; three paired tissues from three patients with prostate cancer were analyzed. (D) Effects of enz on H6PD and 11-HSD2 protein with ex vivo treatment of 12 clinical tissues from patients with prostate cancer. For all panels, unless otherwise noted, error bars represent the SEM, and P values were calculated using unpaired two-tailed t tests.
Anti H6pd Antibody, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/h6pd/H6PD+Mouse+Monoclonal+Antibody/pm34039740-144-11-13
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antibodies against p53  (Santa Cruz Biotechnology)
90
Santa Cruz Biotechnology antibodies against p53
Fig. 1. <t>H6PD</t> and regulation of glucocorticoid metabolism with enz resistance. (A) Effects of enz treatment on H6PD, GR, and 11-HSD2 in the LAPC4 CRPC model as assessed by immunoblot. (B) NADPH generation in LAPC4 cells with enz treatment. NADPH was assessed using a bioluminescence assay in ER extracted from control and enz-treated cells. (C) H6PD and 11-HSD2 protein in tissues from enz-treated patients with prostate cancer. Bar graphs represent band intensity; three paired tissues from three patients with prostate cancer were analyzed. (D) Effects of enz on H6PD and 11-HSD2 protein with ex vivo treatment of 12 clinical tissues from patients with prostate cancer. For all panels, unless otherwise noted, error bars represent the SEM, and P values were calculated using unpaired two-tailed t tests.
Antibodies Against P53, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/h6pd/H6PD+siRNA/pmc06072343-265-21-28
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h6pd  (Santa Cruz Biotechnology)
88
Santa Cruz Biotechnology h6pd
Fig. 1. Effect of starvation on G6PT, <t>H6PD</t> and 11bHSD1 enzyme activities in rat liver microsomes. (A) Activity of the G6PT–H6PD–11bHSD triad. Microsomes were incubated at 37 C in KCl/MOPS buffer in the presence of 10 lM cortisone and 50 lM G6P at a protein concentration of 0.5 mg/ml for 5, 10 and 20 min. The reaction was terminated by addition of ice-cold methanol. The ratio of cortisone– cortisol conversion was analyzed by HPLC (see Section 2). (B) Enzyme activity of H6PD. Microsomes were incubated at room temperature in KCl/MOPS buffer in a fluorimetric quartz cuvette at a protein concentration of 1 mg/ml. Dehydrogenation of G6P was monitored via NADPH formation (extinction and emission wavelengths at 350 and 460 nm, respectively) after the subsequent addition of 2 mM NADP+, 100 lM G6P and 1% Triton X100. Pulse addition of NADPH was done for calibration. All experiments were elaborated in triplicates. The quantified results are shown as mean values ± S.D. Statistical analysis was performed with the Tukey–Kramer Multiple Comparisons Test.
H6pd, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/h6pd/H6PD+Antibody/pm21035447-30-5-11
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h6pd  (OriGene)
90
OriGene h6pd
Fig. 1. Effect of starvation on G6PT, <t>H6PD</t> and 11bHSD1 enzyme activities in rat liver microsomes. (A) Activity of the G6PT–H6PD–11bHSD triad. Microsomes were incubated at 37 C in KCl/MOPS buffer in the presence of 10 lM cortisone and 50 lM G6P at a protein concentration of 0.5 mg/ml for 5, 10 and 20 min. The reaction was terminated by addition of ice-cold methanol. The ratio of cortisone– cortisol conversion was analyzed by HPLC (see Section 2). (B) Enzyme activity of H6PD. Microsomes were incubated at room temperature in KCl/MOPS buffer in a fluorimetric quartz cuvette at a protein concentration of 1 mg/ml. Dehydrogenation of G6P was monitored via NADPH formation (extinction and emission wavelengths at 350 and 460 nm, respectively) after the subsequent addition of 2 mM NADP+, 100 lM G6P and 1% Triton X100. Pulse addition of NADPH was done for calibration. All experiments were elaborated in triplicates. The quantified results are shown as mean values ± S.D. Statistical analysis was performed with the Tukey–Kramer Multiple Comparisons Test.
H6pd, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti g6pdh  (Proteintech)
91
Proteintech anti g6pdh
HA activated pentose phosphate pathway (PPP) via O-GlcNAcylation in hearts. A , Experimental illustration of the O-glycoproteomics analysis of the Control and HA hearts. B , Bubble chart showing Gene Ontology (GO) annotation results. C , The pentose phosphate pathway. D , The levels of reduced nicotinamide adenine dinucleotides phosphate (NADPH) in cardiac tissue lysates (n = 6 per group). E , The NADPH/NADP + ratio in cardiac tissue lysates (n = 6 per group). F , The Glucose-6-phosphate dehydrogenase <t>(G6PDH)</t> activity in cardiac tissue lysates (Control: n = 6, HA: n = 9). G , The levels of glutathione (GSH) in cardiac tissue lysates (n = 5 per group). H , The GSH/GSSG ratio in cardiac tissue lysates (n = 5 per group). Data are shown as mean ± SEM. *P < 0.05, **P < 0.01 for indicated comparisons.
Anti G6pdh, supplied by Proteintech, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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h6pd myc ddk  (OriGene)
90
OriGene h6pd myc ddk
HA activated pentose phosphate pathway (PPP) via O-GlcNAcylation in hearts. A , Experimental illustration of the O-glycoproteomics analysis of the Control and HA hearts. B , Bubble chart showing Gene Ontology (GO) annotation results. C , The pentose phosphate pathway. D , The levels of reduced nicotinamide adenine dinucleotides phosphate (NADPH) in cardiac tissue lysates (n = 6 per group). E , The NADPH/NADP + ratio in cardiac tissue lysates (n = 6 per group). F , The Glucose-6-phosphate dehydrogenase <t>(G6PDH)</t> activity in cardiac tissue lysates (Control: n = 6, HA: n = 9). G , The levels of glutathione (GSH) in cardiac tissue lysates (n = 5 per group). H , The GSH/GSSG ratio in cardiac tissue lysates (n = 5 per group). Data are shown as mean ± SEM. *P < 0.05, **P < 0.01 for indicated comparisons.
H6pd Myc Ddk, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/h6pd/H6PD+(NM_001282587)+Human+Tagged+ORF+Clone/pm34039740-181-25-26
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plasmids  (OriGene)
90
OriGene plasmids
HA activated pentose phosphate pathway (PPP) via O-GlcNAcylation in hearts. A , Experimental illustration of the O-glycoproteomics analysis of the Control and HA hearts. B , Bubble chart showing Gene Ontology (GO) annotation results. C , The pentose phosphate pathway. D , The levels of reduced nicotinamide adenine dinucleotides phosphate (NADPH) in cardiac tissue lysates (n = 6 per group). E , The NADPH/NADP + ratio in cardiac tissue lysates (n = 6 per group). F , The Glucose-6-phosphate dehydrogenase <t>(G6PDH)</t> activity in cardiac tissue lysates (Control: n = 6, HA: n = 9). G , The levels of glutathione (GSH) in cardiac tissue lysates (n = 5 per group). H , The GSH/GSSG ratio in cardiac tissue lysates (n = 5 per group). Data are shown as mean ± SEM. *P < 0.05, **P < 0.01 for indicated comparisons.
Plasmids, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/h6pd/H6PD+(NM_004285)+Human+Tagged+ORF+Clone/pm34039740-147-8-12
Average 90 stars, based on 1 article reviews
plasmids - by Bioz Stars, 2026-10
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g6pd protein purification h6pd  (OriGene)
90
OriGene g6pd protein purification h6pd
HA activated pentose phosphate pathway (PPP) via O-GlcNAcylation in hearts. A , Experimental illustration of the O-glycoproteomics analysis of the Control and HA hearts. B , Bubble chart showing Gene Ontology (GO) annotation results. C , The pentose phosphate pathway. D , The levels of reduced nicotinamide adenine dinucleotides phosphate (NADPH) in cardiac tissue lysates (n = 6 per group). E , The NADPH/NADP + ratio in cardiac tissue lysates (n = 6 per group). F , The Glucose-6-phosphate dehydrogenase <t>(G6PDH)</t> activity in cardiac tissue lysates (Control: n = 6, HA: n = 9). G , The levels of glutathione (GSH) in cardiac tissue lysates (n = 5 per group). H , The GSH/GSSG ratio in cardiac tissue lysates (n = 5 per group). Data are shown as mean ± SEM. *P < 0.05, **P < 0.01 for indicated comparisons.
G6pd Protein Purification H6pd, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/h6pd/H6PD+(NM_004285)+Human+Tagged+ORF+Clone+Lentiviral+Particle/pm34039740-148-2-12
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anti-h6pd  (GeneTex)
90
GeneTex anti-h6pd
HA activated pentose phosphate pathway (PPP) via O-GlcNAcylation in hearts. A , Experimental illustration of the O-glycoproteomics analysis of the Control and HA hearts. B , Bubble chart showing Gene Ontology (GO) annotation results. C , The pentose phosphate pathway. D , The levels of reduced nicotinamide adenine dinucleotides phosphate (NADPH) in cardiac tissue lysates (n = 6 per group). E , The NADPH/NADP + ratio in cardiac tissue lysates (n = 6 per group). F , The Glucose-6-phosphate dehydrogenase <t>(G6PDH)</t> activity in cardiac tissue lysates (Control: n = 6, HA: n = 9). G , The levels of glutathione (GSH) in cardiac tissue lysates (n = 5 per group). H , The GSH/GSSG ratio in cardiac tissue lysates (n = 5 per group). Data are shown as mean ± SEM. *P < 0.05, **P < 0.01 for indicated comparisons.
Anti H6pd, supplied by GeneTex, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/h6pd/anti+h6pd/pmc12166406-54-0-4
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Image Search Results


Bivariate correlations between expression of Vitamin D Receptor ( VDR ) and other skeletal muscle target genes. Data are Spearman correlation coefficients (rho)), with 95% confidence intervals (CI) and p values in brackets).

Journal: PLoS ONE

Article Title: 25-hydroxyvitamin D3 and 1,25-dihydroxyvitamin D3 exert distinct effects on human skeletal muscle function and gene expression

doi: 10.1371/journal.pone.0170665

Figure Lengend Snippet: Bivariate correlations between expression of Vitamin D Receptor ( VDR ) and other skeletal muscle target genes. Data are Spearman correlation coefficients (rho)), with 95% confidence intervals (CI) and p values in brackets).

Article Snippet: Gene targets and assay IDs were as follows: VDR (Hs00172113_m1), CYP27B1 Hs01096154_m1, CYP24A1 Hs00167999_m1, mTOR (Hs00234508_m1), MAFbx/Atrogin-1 (Hs01041408_m1), p300 (Hs00914223_m1), MuRF1 (Hs00261590_m1), Calpain-1 (Hs00559804_m1), Calpain-2 (Hs00965097_m1), USP19 (Hs00324123_m1), ATF4 (Hs00909569_g1), Caspase 3 (Hs00234387_m1), eIF4BP1 (Hs00607050_m1), FOXO1 (Hs01054576_m1), FOXO3 (Hs00818121_m1), MYH1 (Hs00428600_m1), MYH2 (Hs00430042_m1), MYH4 ( Hs00757977_m1 ), myogenin (Hs01072232_m1), SIRT1 (Hs01009005_m1), SIRT3 (Hs00202030_m1), myostatin (Hs00976237_m1), SMAD2 (Hs00183425_m1), SMAD3 (Hs00969210_m1), SMAD4 (Hs00929647_m1), SMAD7 (Hs00998193_m1), ACVR2A (Hs00155658_m1), ACVR2B (Hs00609603_m1), RELA (Hs00153294_m1), RELB (Hs00232399_m1), IL6 (Hs00985639_m1), IL1B (Hs01555410_m1), NFKB1 (Hs00765730_m1), INSR (Hs00961554_m1), IRS1 (Hs00178563_m1), AKT1 (Hs00178289_m1), GSK3B (Hs01047719_m1), DDIT4 (Hs01111686_g1), HSD11B1 (Hs01547870_m1), HSD11B2 (Hs00388669_m1), H6PD ( Hs00188728_m1 ), HSP90AA1 (Hs00743767_sH), HSP90B1 (Hs00427665_g1), GHR (Hs00174872_m1), GHSR (Hs00269780_s1), IGF1 (Hs01547656_m1), HIF1A (Hs00153153_m1), EIF6 (Hs00158272_m1), EIF2B (Hs00426752_m1), PDK4 (Hs01037712_m1), IGF1R (Hs00609566_m1), GADD45A (Hs00169255_m1), ACACA (Hs01046047), AR (Hs00171172_m1), CD36 (Hs00169627_m1), CDNK1 (Hs00355782_m1), CEBP1 (Hs00270923_s1), CRYAB (Hs00157107_m1), CYSC (Hs01588974_g1), GLUL (Hs00365928_g1), HSL (Hs00193510_m1), LPL (Hs00173425_m1), MYCL1 (Hs00420495_m1), MYF5 (Hs00929416_g1), NRC31 (Hs00354508_m1), PPARD (Hs04187066_g1), PPARG (Hs00234592_m1), PPARGC1A (Hs01016719_m1), PPP3R2 (Hs00931245_s1), PSMA2 (Hs00746751_s1), PSMC1 (Hs02386942_g1), PSMC2 (Hs00739800_m1), PSMC4 (Hs00197826_m1), PSMC5 ( Hs01029472_g1), PSMC6 (Hs01652481_g1), PSMD1 (Hs00160631_m1), PSMD2 (Hs01092076_g1), PSMD3 (Hs00160646_m1), PSMD4 (Hs01937833_s1), PSMD6 (Hs00207850_m1), PSMD7 (Hs00427396_m1), PSMD11 (Hs00160660_m1), PSMD12 (Hs00356667_m1), PSMD14 (Hs01113429_m1), RPS6KB1 (Hs00177357_m1), RXRG (Hs00199455_m1), SLC2A4 (Hs00168966_m1), SOCSS3 (Hs00168966_m1), SOD1 (Hs00533490_m1), SREBF1 (Hs01088691_m1), TGFB1 (Hs00998133_m1), TNFA (Hs01113624_g1), TRIM54 (Hs00936695_m1).

Techniques: Expressing

Bivariate correlations between serum 25OHD3, 1α,25(OH) 2 D3 and gene expression in skeletal muscle. Data are Spearman correlation coefficients (rho)), with 95% confidence intervals (CI) and p values in brackets).

Journal: PLoS ONE

Article Title: 25-hydroxyvitamin D3 and 1,25-dihydroxyvitamin D3 exert distinct effects on human skeletal muscle function and gene expression

doi: 10.1371/journal.pone.0170665

Figure Lengend Snippet: Bivariate correlations between serum 25OHD3, 1α,25(OH) 2 D3 and gene expression in skeletal muscle. Data are Spearman correlation coefficients (rho)), with 95% confidence intervals (CI) and p values in brackets).

Article Snippet: Gene targets and assay IDs were as follows: VDR (Hs00172113_m1), CYP27B1 Hs01096154_m1, CYP24A1 Hs00167999_m1, mTOR (Hs00234508_m1), MAFbx/Atrogin-1 (Hs01041408_m1), p300 (Hs00914223_m1), MuRF1 (Hs00261590_m1), Calpain-1 (Hs00559804_m1), Calpain-2 (Hs00965097_m1), USP19 (Hs00324123_m1), ATF4 (Hs00909569_g1), Caspase 3 (Hs00234387_m1), eIF4BP1 (Hs00607050_m1), FOXO1 (Hs01054576_m1), FOXO3 (Hs00818121_m1), MYH1 (Hs00428600_m1), MYH2 (Hs00430042_m1), MYH4 ( Hs00757977_m1 ), myogenin (Hs01072232_m1), SIRT1 (Hs01009005_m1), SIRT3 (Hs00202030_m1), myostatin (Hs00976237_m1), SMAD2 (Hs00183425_m1), SMAD3 (Hs00969210_m1), SMAD4 (Hs00929647_m1), SMAD7 (Hs00998193_m1), ACVR2A (Hs00155658_m1), ACVR2B (Hs00609603_m1), RELA (Hs00153294_m1), RELB (Hs00232399_m1), IL6 (Hs00985639_m1), IL1B (Hs01555410_m1), NFKB1 (Hs00765730_m1), INSR (Hs00961554_m1), IRS1 (Hs00178563_m1), AKT1 (Hs00178289_m1), GSK3B (Hs01047719_m1), DDIT4 (Hs01111686_g1), HSD11B1 (Hs01547870_m1), HSD11B2 (Hs00388669_m1), H6PD ( Hs00188728_m1 ), HSP90AA1 (Hs00743767_sH), HSP90B1 (Hs00427665_g1), GHR (Hs00174872_m1), GHSR (Hs00269780_s1), IGF1 (Hs01547656_m1), HIF1A (Hs00153153_m1), EIF6 (Hs00158272_m1), EIF2B (Hs00426752_m1), PDK4 (Hs01037712_m1), IGF1R (Hs00609566_m1), GADD45A (Hs00169255_m1), ACACA (Hs01046047), AR (Hs00171172_m1), CD36 (Hs00169627_m1), CDNK1 (Hs00355782_m1), CEBP1 (Hs00270923_s1), CRYAB (Hs00157107_m1), CYSC (Hs01588974_g1), GLUL (Hs00365928_g1), HSL (Hs00193510_m1), LPL (Hs00173425_m1), MYCL1 (Hs00420495_m1), MYF5 (Hs00929416_g1), NRC31 (Hs00354508_m1), PPARD (Hs04187066_g1), PPARG (Hs00234592_m1), PPARGC1A (Hs01016719_m1), PPP3R2 (Hs00931245_s1), PSMA2 (Hs00746751_s1), PSMC1 (Hs02386942_g1), PSMC2 (Hs00739800_m1), PSMC4 (Hs00197826_m1), PSMC5 ( Hs01029472_g1), PSMC6 (Hs01652481_g1), PSMD1 (Hs00160631_m1), PSMD2 (Hs01092076_g1), PSMD3 (Hs00160646_m1), PSMD4 (Hs01937833_s1), PSMD6 (Hs00207850_m1), PSMD7 (Hs00427396_m1), PSMD11 (Hs00160660_m1), PSMD12 (Hs00356667_m1), PSMD14 (Hs01113429_m1), RPS6KB1 (Hs00177357_m1), RXRG (Hs00199455_m1), SLC2A4 (Hs00168966_m1), SOCSS3 (Hs00168966_m1), SOD1 (Hs00533490_m1), SREBF1 (Hs01088691_m1), TGFB1 (Hs00998133_m1), TNFA (Hs01113624_g1), TRIM54 (Hs00936695_m1).

Techniques: Gene Expression

Fig. 1. H6PD and regulation of glucocorticoid metabolism with enz resistance. (A) Effects of enz treatment on H6PD, GR, and 11-HSD2 in the LAPC4 CRPC model as assessed by immunoblot. (B) NADPH generation in LAPC4 cells with enz treatment. NADPH was assessed using a bioluminescence assay in ER extracted from control and enz-treated cells. (C) H6PD and 11-HSD2 protein in tissues from enz-treated patients with prostate cancer. Bar graphs represent band intensity; three paired tissues from three patients with prostate cancer were analyzed. (D) Effects of enz on H6PD and 11-HSD2 protein with ex vivo treatment of 12 clinical tissues from patients with prostate cancer. For all panels, unless otherwise noted, error bars represent the SEM, and P values were calculated using unpaired two-tailed t tests.

Journal: Science translational medicine

Article Title: Hexose-6-phosphate dehydrogenase blockade reverses prostate cancer drug resistance in xenograft models by glucocorticoid inactivation.

doi: 10.1126/scitranslmed.abe8226

Figure Lengend Snippet: Fig. 1. H6PD and regulation of glucocorticoid metabolism with enz resistance. (A) Effects of enz treatment on H6PD, GR, and 11-HSD2 in the LAPC4 CRPC model as assessed by immunoblot. (B) NADPH generation in LAPC4 cells with enz treatment. NADPH was assessed using a bioluminescence assay in ER extracted from control and enz-treated cells. (C) H6PD and 11-HSD2 protein in tissues from enz-treated patients with prostate cancer. Bar graphs represent band intensity; three paired tissues from three patients with prostate cancer were analyzed. (D) Effects of enz on H6PD and 11-HSD2 protein with ex vivo treatment of 12 clinical tissues from patients with prostate cancer. For all panels, unless otherwise noted, error bars represent the SEM, and P values were calculated using unpaired two-tailed t tests.

Article Snippet: After incubating with the anti–11-HSD2 antibody (Santa Cruz Biotechnology; 1:3000) and anti-H6PD antibody (OriGene; 1:6000) overnight at 4°C, the appropriate secondary antibody was incubated for 1 hour at room temperature.

Techniques: Western Blot, ATP Bioluminescent Assay, Control, Ex Vivo, Two Tailed Test

Fig. 1. Effect of starvation on G6PT, H6PD and 11bHSD1 enzyme activities in rat liver microsomes. (A) Activity of the G6PT–H6PD–11bHSD triad. Microsomes were incubated at 37 C in KCl/MOPS buffer in the presence of 10 lM cortisone and 50 lM G6P at a protein concentration of 0.5 mg/ml for 5, 10 and 20 min. The reaction was terminated by addition of ice-cold methanol. The ratio of cortisone– cortisol conversion was analyzed by HPLC (see Section 2). (B) Enzyme activity of H6PD. Microsomes were incubated at room temperature in KCl/MOPS buffer in a fluorimetric quartz cuvette at a protein concentration of 1 mg/ml. Dehydrogenation of G6P was monitored via NADPH formation (extinction and emission wavelengths at 350 and 460 nm, respectively) after the subsequent addition of 2 mM NADP+, 100 lM G6P and 1% Triton X100. Pulse addition of NADPH was done for calibration. All experiments were elaborated in triplicates. The quantified results are shown as mean values ± S.D. Statistical analysis was performed with the Tukey–Kramer Multiple Comparisons Test.

Journal: FEBS letters

Article Title: Decreased prereceptorial glucocorticoid activating capacity in starvation due to an oxidative shift of pyridine nucleotides in the endoplasmic reticulum.

doi: 10.1016/j.febslet.2010.10.053

Figure Lengend Snippet: Fig. 1. Effect of starvation on G6PT, H6PD and 11bHSD1 enzyme activities in rat liver microsomes. (A) Activity of the G6PT–H6PD–11bHSD triad. Microsomes were incubated at 37 C in KCl/MOPS buffer in the presence of 10 lM cortisone and 50 lM G6P at a protein concentration of 0.5 mg/ml for 5, 10 and 20 min. The reaction was terminated by addition of ice-cold methanol. The ratio of cortisone– cortisol conversion was analyzed by HPLC (see Section 2). (B) Enzyme activity of H6PD. Microsomes were incubated at room temperature in KCl/MOPS buffer in a fluorimetric quartz cuvette at a protein concentration of 1 mg/ml. Dehydrogenation of G6P was monitored via NADPH formation (extinction and emission wavelengths at 350 and 460 nm, respectively) after the subsequent addition of 2 mM NADP+, 100 lM G6P and 1% Triton X100. Pulse addition of NADPH was done for calibration. All experiments were elaborated in triplicates. The quantified results are shown as mean values ± S.D. Statistical analysis was performed with the Tukey–Kramer Multiple Comparisons Test.

Article Snippet: The polyclonal antibodies against G6PT, H6PD and actin were provided by Santa Cruz Biotechnology.

Techniques: Activity Assay, Incubation, Protein Concentration

Fig. 2. Effect of starvation on G6PT, H6PD and 11bHSD1 protein levels in rat liver microsomes. Protein levels were analyzed by Western blot technique using equal amount (50 lg) of untreated and 12 or 36 h starved rat liver microsomes. Actin was used as internal control. Representative immunoblots of three independent experiments are shown.

Journal: FEBS letters

Article Title: Decreased prereceptorial glucocorticoid activating capacity in starvation due to an oxidative shift of pyridine nucleotides in the endoplasmic reticulum.

doi: 10.1016/j.febslet.2010.10.053

Figure Lengend Snippet: Fig. 2. Effect of starvation on G6PT, H6PD and 11bHSD1 protein levels in rat liver microsomes. Protein levels were analyzed by Western blot technique using equal amount (50 lg) of untreated and 12 or 36 h starved rat liver microsomes. Actin was used as internal control. Representative immunoblots of three independent experiments are shown.

Article Snippet: The polyclonal antibodies against G6PT, H6PD and actin were provided by Santa Cruz Biotechnology.

Techniques: Western Blot, Control

HA activated pentose phosphate pathway (PPP) via O-GlcNAcylation in hearts. A , Experimental illustration of the O-glycoproteomics analysis of the Control and HA hearts. B , Bubble chart showing Gene Ontology (GO) annotation results. C , The pentose phosphate pathway. D , The levels of reduced nicotinamide adenine dinucleotides phosphate (NADPH) in cardiac tissue lysates (n = 6 per group). E , The NADPH/NADP + ratio in cardiac tissue lysates (n = 6 per group). F , The Glucose-6-phosphate dehydrogenase (G6PDH) activity in cardiac tissue lysates (Control: n = 6, HA: n = 9). G , The levels of glutathione (GSH) in cardiac tissue lysates (n = 5 per group). H , The GSH/GSSG ratio in cardiac tissue lysates (n = 5 per group). Data are shown as mean ± SEM. *P < 0.05, **P < 0.01 for indicated comparisons.

Journal: Redox Biology

Article Title: Hypoxic acclimation improves cardiac redox homeostasis and protects heart against ischemia-reperfusion injury through upregulation of O-GlcNAcylation

doi: 10.1016/j.redox.2021.101994

Figure Lengend Snippet: HA activated pentose phosphate pathway (PPP) via O-GlcNAcylation in hearts. A , Experimental illustration of the O-glycoproteomics analysis of the Control and HA hearts. B , Bubble chart showing Gene Ontology (GO) annotation results. C , The pentose phosphate pathway. D , The levels of reduced nicotinamide adenine dinucleotides phosphate (NADPH) in cardiac tissue lysates (n = 6 per group). E , The NADPH/NADP + ratio in cardiac tissue lysates (n = 6 per group). F , The Glucose-6-phosphate dehydrogenase (G6PDH) activity in cardiac tissue lysates (Control: n = 6, HA: n = 9). G , The levels of glutathione (GSH) in cardiac tissue lysates (n = 5 per group). H , The GSH/GSSG ratio in cardiac tissue lysates (n = 5 per group). Data are shown as mean ± SEM. *P < 0.05, **P < 0.01 for indicated comparisons.

Article Snippet: Membrane was blocked in 5% non-fat milk and incubated with primary antibodies at 4 °C overnight, including anti- O -GlcNAC (#ab2739, Abcam), anti-OGT (#11576-2-AP, Proteintech, China), anti-OGA (#14711-1-AP, Proteintech), anti-GFAT1 (#14132-1-AP, Proteintech), anti-GALE (#14414-1-AP, Proteintech), anti-HK2 (#22029-1-AP, Proteintech), anti-IL-1β (#AF4006, Affbiotech, China), anti-IL-6 (#DF6087, Affbiotech), anti-G6PDH (#DF6444, Affbiotech), anti-GAPDH (#10494-1-AP, Proteintech), anti-Tubulin (#11224-1-AP, Proteintech) and anti-β-actin (#20536-1-AP, Proteintech) antibodies.

Techniques: Activity Assay

PPP was enhanced via O-GlcNAcylation-mediated activation of G6PDH. A , Representative blots of G6PDH in cardiac tissues and H9c2 cells (n = 4 per group). B–C , Representative blots of O-GlcNAc modified G6PDH ( B ) and G6PDH activity ( C ) in H9c2 cells treated with IL-1β for 4 or 24 h (n = 6 per group). D-E , Representative blots of O-GlcNAc modification ( D ) and the G6PDH activity ( E ) in H9c2 cells treated with IL-6 for 4 or 24 h (n = 6 per group). F , The compounds used in this study for targeting HBP pathway enzymes. G-I , Representative blots of O-GlcNAc-modified G6PDH ( G ), G6PDH activity ( H ), and NADPH/NADP + ratio ( I ) in H9c2 cells with indicated treatments (n = 4–5 per group). J , Representative blots of O-GlcNAc modified G6PDH in the mouse hearts with indicated treatments (n = 3 per group). K-M , The G6PDH activity ( K ), NADPH/NADP + ratio ( L ), and GSH/GSSG ratio ( M ) in the mouse hearts with indicated treatments (n = 4–6 per group). Data are shown as mean ± SEM. *P < 0.05, **P < 0.01 and ***P < 0.001 for indicated comparisons. NS: no significant difference.

Journal: Redox Biology

Article Title: Hypoxic acclimation improves cardiac redox homeostasis and protects heart against ischemia-reperfusion injury through upregulation of O-GlcNAcylation

doi: 10.1016/j.redox.2021.101994

Figure Lengend Snippet: PPP was enhanced via O-GlcNAcylation-mediated activation of G6PDH. A , Representative blots of G6PDH in cardiac tissues and H9c2 cells (n = 4 per group). B–C , Representative blots of O-GlcNAc modified G6PDH ( B ) and G6PDH activity ( C ) in H9c2 cells treated with IL-1β for 4 or 24 h (n = 6 per group). D-E , Representative blots of O-GlcNAc modification ( D ) and the G6PDH activity ( E ) in H9c2 cells treated with IL-6 for 4 or 24 h (n = 6 per group). F , The compounds used in this study for targeting HBP pathway enzymes. G-I , Representative blots of O-GlcNAc-modified G6PDH ( G ), G6PDH activity ( H ), and NADPH/NADP + ratio ( I ) in H9c2 cells with indicated treatments (n = 4–5 per group). J , Representative blots of O-GlcNAc modified G6PDH in the mouse hearts with indicated treatments (n = 3 per group). K-M , The G6PDH activity ( K ), NADPH/NADP + ratio ( L ), and GSH/GSSG ratio ( M ) in the mouse hearts with indicated treatments (n = 4–6 per group). Data are shown as mean ± SEM. *P < 0.05, **P < 0.01 and ***P < 0.001 for indicated comparisons. NS: no significant difference.

Article Snippet: Membrane was blocked in 5% non-fat milk and incubated with primary antibodies at 4 °C overnight, including anti- O -GlcNAC (#ab2739, Abcam), anti-OGT (#11576-2-AP, Proteintech, China), anti-OGA (#14711-1-AP, Proteintech), anti-GFAT1 (#14132-1-AP, Proteintech), anti-GALE (#14414-1-AP, Proteintech), anti-HK2 (#22029-1-AP, Proteintech), anti-IL-1β (#AF4006, Affbiotech, China), anti-IL-6 (#DF6087, Affbiotech), anti-G6PDH (#DF6444, Affbiotech), anti-GAPDH (#10494-1-AP, Proteintech), anti-Tubulin (#11224-1-AP, Proteintech) and anti-β-actin (#20536-1-AP, Proteintech) antibodies.

Techniques: Activation Assay, Modification, Activity Assay

A schematic illustration of the working model of this study . The present study demonstrated that hypoxia acclimation-associated inflammatory cytokines upregulate the hexosamine biosynthetic pathway (HBP) and activate G6PDH by increasing the O-GlcNAcylation, which improves pentose phosphate pathway (PPP)-mediated redox homeostasis, and alleviates acute cardiac I/R injury.

Journal: Redox Biology

Article Title: Hypoxic acclimation improves cardiac redox homeostasis and protects heart against ischemia-reperfusion injury through upregulation of O-GlcNAcylation

doi: 10.1016/j.redox.2021.101994

Figure Lengend Snippet: A schematic illustration of the working model of this study . The present study demonstrated that hypoxia acclimation-associated inflammatory cytokines upregulate the hexosamine biosynthetic pathway (HBP) and activate G6PDH by increasing the O-GlcNAcylation, which improves pentose phosphate pathway (PPP)-mediated redox homeostasis, and alleviates acute cardiac I/R injury.

Article Snippet: Membrane was blocked in 5% non-fat milk and incubated with primary antibodies at 4 °C overnight, including anti- O -GlcNAC (#ab2739, Abcam), anti-OGT (#11576-2-AP, Proteintech, China), anti-OGA (#14711-1-AP, Proteintech), anti-GFAT1 (#14132-1-AP, Proteintech), anti-GALE (#14414-1-AP, Proteintech), anti-HK2 (#22029-1-AP, Proteintech), anti-IL-1β (#AF4006, Affbiotech, China), anti-IL-6 (#DF6087, Affbiotech), anti-G6PDH (#DF6444, Affbiotech), anti-GAPDH (#10494-1-AP, Proteintech), anti-Tubulin (#11224-1-AP, Proteintech) and anti-β-actin (#20536-1-AP, Proteintech) antibodies.

Techniques: