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Merck KGaA
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GeneTex
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EpiGentek
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AnaSpec
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Image Search Results
Journal: Genetics
Article Title: Distinct requirements for Pho, Sfmbt, and Ino80 for cell survival in Drosophila
doi: 10.1093/genetics/iyab096
Figure Lengend Snippet: Cell proliferation is unimpaired in pho phol , Sfmbt or Ino80 mutants but pho phol mutants show extensive apoptotic cell death. (A) Top: wing imaginal discs of third-instar larvae of the indicated genotypes, stained with antibody against H3S10ph and Hoechst (DNA) to label all nuclei. Below: Normalized number of H3S10ph-positive cells per area (10,000 µm 2 ) of imaginal wing disc tissue in each genotype. For each genotype, bars represent the mean number and standard deviation of cells counted in discs from six different individuals. (B) Top: wing imaginal discs like in (A), stained with antibody against cDcp-1 and Hoechst (DNA). Below: Normalized numbers of the cDcp-1-positive nuclei per area (10,000 µm 2 ) of imaginal wing disc tissue in each genotype. For each genotype, histogram bars represent the mean number and standard deviation of cells counted in discs from six different individuals.
Article Snippet: The following antibodies were used for immunostainings: mouse anti-Abd-B clone 1A2E9 (1:200) (Developmental Studies Hybridoma Bank), mouse anti-Antp clone 8C11 (1:100) (Developmental Studies Hybridoma Bank), rabbit anti-cDcp-1 (1:300) (#9578; Cell Signalling),
Techniques: Staining, Standard Deviation
Journal: Cell Reports
Article Title: Interplay of Histone Marks with Serine ADP-Ribosylation
doi: 10.1016/j.celrep.2018.08.092
Figure Lengend Snippet: H3S10ADPr Reduces the Efficiency of Subsequent H3K9 Acetylation and H3S10 Phosphorylation (A) Western blot showing PARP1/HPF1 ADPr of H3 (1–20aa) peptide and subsequent p300-mediated acetylation. One reaction was stopped after p300 incubation, then supplemented with ARH3 to remove ADPr before signal detection. Membrane probed with H3K9ac antibody, with H3K9ac peptide included as a positive marker. (B) Western blot showing PARP1/HPF1 ADPr of H3 (1–20aa) peptide and subsequent Baronase-mediated phosphorylation. Control sample excludes NAD from the PARP1/HPF1 reaction. Membrane probed with H3S10ph antibody, with H3S10ph peptide included as a positive marker. (C) Western blot showing PARP1/HPF1 ADPr of H3 (1–20aa) peptide and subsequent Dim5-mediated methylation. Control sample excludes NAD from the PARP1/HPF1 reaction. Membrane probed with H3K9me3 antibody, with H3K9me3 peptide included as a positive marker.
Article Snippet:
Techniques: Phospho-proteomics, Western Blot, Incubation, Membrane, Marker, Control, Methylation
Journal: Cell Reports
Article Title: Interplay of Histone Marks with Serine ADP-Ribosylation
doi: 10.1016/j.celrep.2018.08.092
Figure Lengend Snippet:
Article Snippet:
Techniques: Recombinant, Staining, Plasmid Preparation, Software, Mass Spectrometry, Modification