Journal: Cancers

Article Title: Non-Secreted Mature Decoy-Resistant IL-18-Armed Oncolytic Vaccinia Virus Elicits Potent Antitumor Effects in an Aggressive Murine Ovarian Cancer Model

doi: 10.3390/cancers18071065

Figure Lengend Snippet: nsmDR-18 expression via oVV does not impair viral replication in vitro and in vivo. Tumor cells (ID8a-luc, 2.0 × 10 5 cells; A2780, 3.0 × 10 5 cells), were treated with PBS or infected with vvDD or vvDD-nsmDR-18 at an MOI of 1. Cells were stained with Hoechst 33342 and imaged. Representative fluorescence images of cells infected with YFP-expressing virus (yellow) and counterstained with Hoechst 33342 (blue) to visualize nuclei ( A , C ). Cells were harvested to measure A34R or nsmDR-18 expression using RT-qPCR 24 h posttreatment ( B , D ). B6 mice were inoculated i.p. with 3.5 × 10 6 ID8a-luc cells and treated i.p. with the indicated viruses (1.0 × 10 8 PFU/mouse) or PBS five days after tumor cell inoculation (4–6 mice/group). Tumors were harvested for RT-qPCR analysis. Expression of viral gene A34R and nsmDR-18 is shown in ( E ). Data show one representative experiment, except for panels in ( E ), which present combined data from two independent experiments. p < 0.05 (*); p < 0.001 (***), and p < 0.0001 (****); ns indicates not significant.

Article Snippet: The cells were cultured for 24 h post-treatment and then incubated with Hoechst 33342 dye (Biotium, Fremont, CA, USA, #40046; 1 ug/mL) for 5–15 min before imaging.

Techniques: Expressing, In Vitro, In Vivo, Infection, Staining, Fluorescence, Virus, Quantitative RT-PCR

Journal: Cancers

Article Title: Non-Secreted Mature Decoy-Resistant IL-18-Armed Oncolytic Vaccinia Virus Elicits Potent Antitumor Effects in an Aggressive Murine Ovarian Cancer Model

doi: 10.3390/cancers18071065

Figure Lengend Snippet: nsmDR-18 expression via oVV does not impair viral cytotoxicity. Tumor cells (ID8a-luc, 5.0 × 10 3 cells; A2780, 1.0 × 10 4 cells), were treated with PBS or infected with vvDD or vvDD-nsmDR-18 at indicated MOIs. Cells were stained with Hoechst 33342 and imaged. Representative fluorescence images of cells infected with YFP-expressing virus (yellow) and counterstained with Hoechst 33342 (blue) to visualize nuclei ( A , B ). Cell viability was measured 48 h post-infection ( C , D ). Data show one representative experiment.

Article Snippet: The cells were cultured for 24 h post-treatment and then incubated with Hoechst 33342 dye (Biotium, Fremont, CA, USA, #40046; 1 ug/mL) for 5–15 min before imaging.

Techniques: Expressing, Infection, Staining, Fluorescence, Virus

Journal: Toxics

Article Title: Comparison of Simultaneous Quantitative Analysis of Methylmercury and Inorganic Mercury in Cord Blood Using LC-ICP-MS and LC-CVAFS: The Pilot Study of the Japan Environment and Children’s Study

doi: 10.3390/toxics9040082

Figure Lengend Snippet: Concentrations of methylmercury and inorganic mercury in blood samples from the Japan Environment and Children’s Study (JECS) pilot study.

Article Snippet: A standard solution of methylmercury chloride (1000 mg/L) was obtained from Alfa Aesar (Ward Hill, MA, USA).

Techniques:

Journal: Toxics

Article Title: Comparison of Simultaneous Quantitative Analysis of Methylmercury and Inorganic Mercury in Cord Blood Using LC-ICP-MS and LC-CVAFS: The Pilot Study of the Japan Environment and Children’s Study

doi: 10.3390/toxics9040082

Figure Lengend Snippet: Interlaboratory comparison of the concentrations of methylmercury, inorganic mercury, Japan Environment and Children’s Study (JECS and total mercury in blood using reference materials.

Article Snippet: A standard solution of methylmercury chloride (1000 mg/L) was obtained from Alfa Aesar (Ward Hill, MA, USA).

Techniques:

Journal: Toxics

Article Title: Comparison of Simultaneous Quantitative Analysis of Methylmercury and Inorganic Mercury in Cord Blood Using LC-ICP-MS and LC-CVAFS: The Pilot Study of the Japan Environment and Children’s Study

doi: 10.3390/toxics9040082

Figure Lengend Snippet: Correlation matrix plots between mercury concentrations (ng/g) in cord blood and maternal blood. Correlation matrix plots show the distribution, ‘ rho ’ of Spearman’s correlation coeffi-cients, and scatter plots. LC-ICP-MS: liquid chromatography-inductively coupled plasma mass spectrometry. Mercury speciation results obtained by LC-ICP-MS are shown as methylmercury (MeHg), inorganic mercury (IHg), and the sum (MeHg + IHg). Total mercury (THg) results ob-tained by ICP-MS are showed as THg. The plots of more than method detection limit are shown.

Article Snippet: A standard solution of methylmercury chloride (1000 mg/L) was obtained from Alfa Aesar (Ward Hill, MA, USA).

Techniques: Liquid Chromatography, Mass Spectrometry

Journal: Toxics

Article Title: Comparison of Simultaneous Quantitative Analysis of Methylmercury and Inorganic Mercury in Cord Blood Using LC-ICP-MS and LC-CVAFS: The Pilot Study of the Japan Environment and Children’s Study

doi: 10.3390/toxics9040082

Figure Lengend Snippet: Relationships between cord blood:maternal blood ratios of inorganic mercury (IHg) and methylmercury (MeHg). Y = 0.07 + 0.40 × ( p < 0.001).

Article Snippet: A standard solution of methylmercury chloride (1000 mg/L) was obtained from Alfa Aesar (Ward Hill, MA, USA).

Techniques: