Journal: bioRxiv

Article Title: LIMD1 Loss Confers a GPX4-Dependent Cell State in Lung Cancer

doi: 10.1101/2025.06.06.658274

Figure Lengend Snippet: (A) Representative images of isogenic H1299 cells stained for lipid droplets with Oil Red O upon 24 h treatment with vehicle and BSA-conjugated Oleate (100 µM) (n=3). (B) Quantification of basal lipid droplet levels in isogenic H1299 cells. (C) ROS levels evaluated by H2DCFDA staining in isogenic H1299 cells following seeding in 6-well plates at a confluency of 1.25×10 6 cells per well (n=3). (D, E) Lipid peroxidation levels evaluated by C11-BODIPY 581/591 staining in isogenic H1299 cells under basal conditions (n=4) (D) and upon treatment (E) with RSL3 (8 nM, 10 nM, 20 nM), fer-1 (2 μM) and RSL3 (20 nM) combined with fer-1 (2 μM) for 24 h (n=3). (F) ROS levels evaluated by H2DCFDA staining in isogenic SAEC cells following seeding in 6-well plates at a confluency of 1.25×10 6 cells per well (n=3). (G, H) Lipid peroxidation levels evaluated by C11-BODIPY 581/591 staining in isogenic SAEC cells under basal conditions (n=4) (G) and upon treatment (H) with ML210 (0.1 µM, 0.2 µM), fer-1 (2 μM) for 24 h; H 2 O 2 (300 μM) and H 2 O 2 (300 μM) with fer-1 (2 μM) treatment was performed for the last 30 minutes (n=3). Data is shown as biological replicates ± S.D. in C-H and as biological replicates ± S.E.M. in B . One-way ANOVA with Dunnett’s multiple comparisons test was performed in B-D, F , whereas Two-way ANOVA with Bonferroni’s multiple comparisons test was performed in E and H . Two-tailed unpaired t-test was performed in G . * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001.

Article Snippet: Cells were trypsinised, neutralised with 2% FBS in PBS (FACS buffer), pelleted, and resuspended in 500 μL FACS buffer containing either 2 μM BODIPY 581/591 C11 (ThermoFisher #D3861) or 100 μM H2DCFDA (Tocris #5935).

Techniques: Staining, Two Tailed Test

Journal: International Wound Journal

Article Title: Topical effect of benzalkonium bromide on wound healing and potential cellular and molecular mechanisms

doi: 10.1111/iwj.13555

Figure Lengend Snippet: Viability and reactive oxygen species (ROS) production analysis of BB, PVP‐I, CHG treated cells. A, Growth inhibition curves of cells based on CCK‐8 analysis. B, ROS production of cells detected by flow cytometry. C, Mean fluorescence intensity of H2DCFDA probe in cells. D, Fluorescence images of cells after H2DCFDA probe staining. ** P < .01; *** P < .001

Article Snippet: ROS Fluorescent indicator (H2DCFDA) was purchased from Tocris Bioscience (Bristol, United Kingdom).

Techniques: Inhibition, CCK-8 Assay, Flow Cytometry, Fluorescence, Staining