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Image Search Results
Journal: Journal of the Peripheral Nervous System
Article Title: A Next‐Generation ELISA for the Detection of Anti‐(Para)Nodal Antibodies in Autoimmune Nodopathy and COVID ‐19 Vaccinated Individuals
doi: 10.1111/jns.70117
Figure Lengend Snippet: Ella for the detection of anti‐(para)nodal antibodies. (A) Schematic display of the Ella procedure, including hands‐on time for sample dilution and pipetting, and hands‐free time during the automated Ella run. Abbreviations: C = capture protein, D = detection antibody, GNR = glass nanoreactor, S = sample (human serum). Created with BioRender.com (B) Mean RFU values after serum‐specific background normalization in healthy controls (HC), seronegative (SN) and seropositive (SP) patients, using neurofascin‐155 (NF155), contactin‐1 (CNTN1), Caspr‐1, and neurofascin‐186 (NF186) as target proteins. (C) Spearman correlation of serum titers of n = 9 AN patients by Ella ( y ‐axis) and ELISA ( x ‐axis) on a logarithmic scale. “2 × ” represents two different patient samples with identical titers (1:2000 in both assays). The line shows the linear regression curve. ** p ≤ 0.01 (D) Intra‐Assay coefficient of variation (CV) in six samples, compared between Ella and ELISA. N.s. = not significant. (E) Inter‐Assay CV in six samples, compared between Ella and ELISA. N.s. = not significant.
Article Snippet: Protein ,
Techniques: Enzyme-linked Immunosorbent Assay, Intra Assay, Inter Assay
Journal: Journal of the Peripheral Nervous System
Article Title: A Next‐Generation ELISA for the Detection of Anti‐(Para)Nodal Antibodies in Autoimmune Nodopathy and COVID ‐19 Vaccinated Individuals
doi: 10.1111/jns.70117
Figure Lengend Snippet: Screening for anti‐(para)nodal antibodies in SARS‐CoV‐2 vaccinated individuals and follow‐up. (A) Mean RFU values in the cohort of SARS‐CoV‐2‐vaccinated or infected individuals for anti‐NF155 (neurofascin‐155, left) and anti‐CNTN1 (Contactin‐1, right) antibodies, with means and standard deviation as error bars. Thresholds for positive results after normalization are indicated by the red line. Although many samples show high RFU in raw data, only one patient shows a positive result for NF155 after normalization (red square). (B) Clinical data including vaccination time points (syringe), Omicron infection (PCR), anti‐NF155 mean RFU values (left y ‐axis, black), and anti‐SARS‐CoV‐2 spike IgG levels (right y ‐axis, grey) of serum follow‐up samples over 25 months ( x ‐axis) in the Ella NF155 positive patient. (C) Serum binding assays with neurofascin‐transfected HEK293 cells show no specific binding of patient IgG diluted 1:100 (magenta, left) nor colocalization with anti‐pan‐neurofascin‐labeled, transfected cells (arrowheads, green). Overlay/merge is shown with DAPI staining on the right. Scale bar = 10 μm. (D) Murine teased fibers serum binding assays show IgG deposition at the nodal region (arrows) in follow‐up serum 3 (left) and 4 (middle) of the Ella NF155 positive patient, but not fully colocalizing with the paranodal NF155‐fraction when performing double staining with commercial anti‐pan‐neurofascin (green, right; patient IgG shown in magenta). Scale bar = 10 μm.
Article Snippet: Protein ,
Techniques: Infection, Standard Deviation, Binding Assay, Transfection, Labeling, Staining, Double Staining