Journal: Neuroscience

Article Title: Nogo presence is inversely associated with shifts in cortical microglial morphology following experimental diffuse brain injury

doi: 10.1016/j.neuroscience.2017.07.027

Figure Lengend Snippet: Representative images of immunohistochemistry for Iba-1 (microglia) show the changes in microglial morphologies in sham and brain-injured animals over time in the S1BF and S2 cortical regions. Rod microglia appeared to be most prevalent in the S1BF at 2 hours, 6 hours and 7 days post-injury (A). Cells were counted by their morphologies outlined in figure 2. Proportions of microglial morphologies in the population in the S1BF and S2 revealed changes over the post-injury time course. Graphs show mean proportions of macrophages (blue), activated (red), rod (purple) and ramified (green) microglia at each time point in the S1BF and S2 (B). Rod microglia were not present in sham brains, but were observed in the S1BF at every post-injury time point. Rod microglia were detectable in the S2 (C).

Article Snippet: Sections were then incubated at 4ºC overnight in 1% blocking solution containing mouse anti-Iba-1/AIF (Millipore, cat #MABN92 1:1000) or rabbit anti-Iba-1 and one of the following: rabbit anti-CNPase (Cell Signaling, cat #5664 1:500), rabbit anti-Nogo (Abcam, cat #ab32298 1:2000) or mouse anti-myelin basic protein (MBP; Covance, cat #SMI-99P).

Techniques: Immunohistochemistry

Journal: Neuroscience

Article Title: Nogo presence is inversely associated with shifts in cortical microglial morphology following experimental diffuse brain injury

doi: 10.1016/j.neuroscience.2017.07.027

Figure Lengend Snippet: Representative 20× images of double labelling immunofluorescence for Iba-1 (microglia) and CNPase (myelin) show that decreased myelin antigenicity was most evident in the S1BF and S2 at 1 day post-injury, indicated by a reduced quality of CNPase staining. Staining quality for CNPase returned by 2 days post-injury, and appeared most similar to sham at 21 days post-injury. Decreased myelin antigenicity did not occur in close proximity to rod microglia, rather changes in CNPase antigenicity were widespread. All sections were stained in a single batch.

Article Snippet: Sections were then incubated at 4ºC overnight in 1% blocking solution containing mouse anti-Iba-1/AIF (Millipore, cat #MABN92 1:1000) or rabbit anti-Iba-1 and one of the following: rabbit anti-CNPase (Cell Signaling, cat #5664 1:500), rabbit anti-Nogo (Abcam, cat #ab32298 1:2000) or mouse anti-myelin basic protein (MBP; Covance, cat #SMI-99P).

Techniques: Immunofluorescence, Staining

Journal: Neuroscience

Article Title: Nogo presence is inversely associated with shifts in cortical microglial morphology following experimental diffuse brain injury

doi: 10.1016/j.neuroscience.2017.07.027

Figure Lengend Snippet: Representative 20× images of double labelling immunofluorescence for Iba-1 (microglia) and MBP (myelin) show that decreased myelin antigenicity was most evident in the S1BF and S2 at 1 day post-injury, indicated by a reduced quality of MBP staining. Staining quality for MBP returned by 7 days post-injury. Insets (40×) show rod microglia aligning to myelinated axons at 2 hours and 7 days post-injury in the S1BF. Decreased myelin antigenicity did not occur in close proximity to rod microglia.

Article Snippet: Sections were then incubated at 4ºC overnight in 1% blocking solution containing mouse anti-Iba-1/AIF (Millipore, cat #MABN92 1:1000) or rabbit anti-Iba-1 and one of the following: rabbit anti-CNPase (Cell Signaling, cat #5664 1:500), rabbit anti-Nogo (Abcam, cat #ab32298 1:2000) or mouse anti-myelin basic protein (MBP; Covance, cat #SMI-99P).

Techniques: Immunofluorescence, Staining

Journal: Neuroscience

Article Title: Nogo presence is inversely associated with shifts in cortical microglial morphology following experimental diffuse brain injury

doi: 10.1016/j.neuroscience.2017.07.027

Figure Lengend Snippet: Representative images of double labelling immunofluorescence for Iba-1 (microglia) and Nogo A/B revealed Nogo in low levels within sham-injured brain, but began to accumulate in the S1BF at 6 hours post-injury and remained until 2 days post-injury (arrows). This coincided with fewer rod microglia (A). Nogo was occasionally present in the S2 between 6 hours and 2 days post-injury (arrow) (A). Some activated microglia and macrophages co-labelled for Nogo in the S1BF, suggesting that these cells were clearing Nogo from the area by phagocytosis (B).

Article Snippet: Sections were then incubated at 4ºC overnight in 1% blocking solution containing mouse anti-Iba-1/AIF (Millipore, cat #MABN92 1:1000) or rabbit anti-Iba-1 and one of the following: rabbit anti-CNPase (Cell Signaling, cat #5664 1:500), rabbit anti-Nogo (Abcam, cat #ab32298 1:2000) or mouse anti-myelin basic protein (MBP; Covance, cat #SMI-99P).

Techniques: Immunofluorescence

Journal: Neuroscience

Article Title: Nogo presence is inversely associated with shifts in cortical microglial morphology following experimental diffuse brain injury

doi: 10.1016/j.neuroscience.2017.07.027

Figure Lengend Snippet: Representative images of Immunohistochemistry for Iba-1 (microglia) revealed infiltration of amoeboid microglia/macrophages (black arrow heads) at the edge of the S1BF at 2 days post-injury in NEP(1-40) treated brains. Although regions analysed at 20× in the inner S1BF in vehicle (Ai) and NEP(1-40) (Bi) treated brains revealed similar macrophage proportions, outer regions in vehicle (Aii) and NEP(1-40) (Bii) brains appeared to have different macrophage proportions. This qualitative evidence suggests that NEP(1-40) may promote infiltration of amoeboid microglia/macrophages at the edge of the S1BF.

Article Snippet: Sections were then incubated at 4ºC overnight in 1% blocking solution containing mouse anti-Iba-1/AIF (Millipore, cat #MABN92 1:1000) or rabbit anti-Iba-1 and one of the following: rabbit anti-CNPase (Cell Signaling, cat #5664 1:500), rabbit anti-Nogo (Abcam, cat #ab32298 1:2000) or mouse anti-myelin basic protein (MBP; Covance, cat #SMI-99P).

Techniques: Immunohistochemistry

Journal: Studies in Mycology

Article Title: A taxonomic and phylogenetic revision of Penicillium section Aspergilloides

doi: 10.1016/j.simyco.2014.09.002

Figure Lengend Snippet: Strains used in this study.

Article Snippet: P. montanense , DTO 090-I6 = CBS 310.63 = ATCC 14941 = FRR 3407 = IFO 7740 = IHEM 4375 = IMI 099468 = MUCL 31326 = NRRL 3407 = DAOM 226282 , Coniferous forest soil; Ravalli Co., Montana, USA; ex-type of P. montanense , KM189551 , KM088789 , KM089561 , KM089174.

Techniques: Western Blot, Isolation

Journal: Studies in Mycology

Article Title: A taxonomic and phylogenetic revision of Penicillium section Aspergilloides

doi: 10.1016/j.simyco.2014.09.002

Figure Lengend Snippet: Overview of diagnostic characters of species belonging to the P. fuscum -clade.

Article Snippet: P. montanense , DTO 090-I6 = CBS 310.63 = ATCC 14941 = FRR 3407 = IFO 7740 = IHEM 4375 = IMI 099468 = MUCL 31326 = NRRL 3407 = DAOM 226282 , Coniferous forest soil; Ravalli Co., Montana, USA; ex-type of P. montanense , KM189551 , KM088789 , KM089561 , KM089174.

Techniques: Diagnostic Assay