Journal: Lasers in Medical Science

Article Title: Contrasting biological responses of gingival fibroblasts and keratinocyte to blue and violet light irradiation: implications for photobiomodulation use in the therapeutic management of periodontal disease

doi: 10.1007/s10103-026-04817-4

Figure Lengend Snippet: Western blot and densitometry analysis of NQO1 (A, D) GSR (B, E) and KEAP1 (C, F) protein levels in pHGFs following blue light (457 nm) treatment. Analysis was performed on cell lysatesretrieved 6 h (top half of image) and 24 h (lower half of image) post-treatment. Western blot band intensity was normalised to the β-actin loading control, before calculating percentage changes in band intensity relative to untreated controls, represented by the dashed line. N=3, mean ± SD.All p>0.05 .

Article Snippet: Glutathione Disulfide Reductase , GSR , Hs00167317_m1.

Techniques: Western Blot, Control

Journal: Lasers in Medical Science

Article Title: Contrasting biological responses of gingival fibroblasts and keratinocyte to blue and violet light irradiation: implications for photobiomodulation use in the therapeutic management of periodontal disease

doi: 10.1007/s10103-026-04817-4

Figure Lengend Snippet: Evaluation of the role which ROS play in regulating antioxidant gene expression in pHGFs post-blue light treatment. pHGFs were irradiated with 457 nm blue light in the presence and absence of 10 mM NAC. mRNA was isolated from pHGFs 6 h post-blue light treatment. GSR gene expression was normalised to the expression of GSR in non-irradiated cultures, which had received the same 10 mM NAC treatment. N = 3, mean ± SD

Article Snippet: Glutathione Disulfide Reductase , GSR , Hs00167317_m1.

Techniques: Gene Expression, Irradiation, Isolation, Expressing

Journal: Biomedical Reports

Article Title: Effect of light on global gene expression in the neuroglobin-deficient mouse retina

doi: 10.3892/br.2014.364

Figure Lengend Snippet: TaqMan ® assays used for reverse transcription quantitative polymerase chain reaction gene expression validation.

Article Snippet: Expression levels were calculated using the ΔCt method ( 24 ), where the Ct of the gene of interest is normalized to a reference gene. table ft1 table-wrap mode="anchored" t5 caption a7 Gene symbol Gene name Assay identity or sequence Hif1α Hypoxia inducible factor 1α Mm00468879_g Bnip3 BCL2/adenovirus E1B 19 kDa interacting protein 3 Mm01275601_g Gstz1 Glutatione S-transferase Mm01296880_m1 Gsr Glutatione reductase Mm00439154_m1 Xbp1 X-box binding protein 1 Mm00457357_m1 Nfe2l2 ( Nrf2 ) Nuclear factor (erythroid-derived 2)-like 2 Mm00477784_m1 Egr1 Early growth response protein 1 Mm00656724_m1 Fos FBJ osteosarcoma oncogene Mm00487425_m1 Akap6 A kinase (PRKA) anchor protein 6 Mm01292745_m1 Atp8a2 ATPase, aminophospholipid transporter, class I, type 8A, member 2 Mm00443740_m1 Entpd4 Ectonucleoside triphosphate diphosphohydrolase 4 Mm00491888_m1 Hprt1 Hypoxanthine phosphoribosyltransferase 1 F 5′-GCAGTACAGCCCCAAAATGG-3′, R 5′-AACAAA GTCTGGCCTGTATCCAA-3′, probe (VIC) 5′-VIC-AAGCTTGCTGGTGAAAAGGACCTCTCG-3′ Open in a separate window F, forward; R, reverse.

Techniques: Reverse Transcription, Real-time Polymerase Chain Reaction, Gene Expression, Biomarker Discovery, Sequencing, Binding Assay