gsk Search Results


  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94
    Novartis gsk
    Principal Component Analysis plots. Chemical diversity of the <t>GSK,</t> <t>Novartis</t> and St Jude libraries displayed (Panel A); Overlap in chemical diversity of the combined datasets and the commercially available compounds (Panel B); Overlap in chemical diversity of the commercially available compounds where the drug-like and probe-like chemotypes were annotated (Panel C).
    Gsk, supplied by Novartis, used in various techniques. Bioz Stars score: 94/100, based on 2107 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gsk/product/Novartis
    Average 94 stars, based on 2107 article reviews
    Price from $9.99 to $1999.99
    gsk - by Bioz Stars, 2020-09
    94/100 stars
      Buy from Supplier

    94
    Glaxo Smith glaxosmithkline gsk
    Modified PRISMA (preferred reporting items for systematic reviews and meta-analyses) flowchart of search showing trials identified through literature search, trials requested from <t>GSK</t> CSDR.com, and those identified through GSK Study Register. CSDR.com=ClinicalStudyDataRequest.com; <t>GSK=GlaxoSmithKline</t>
    Glaxosmithkline Gsk, supplied by Glaxo Smith, used in various techniques. Bioz Stars score: 94/100, based on 2304 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/glaxosmithkline gsk/product/Glaxo Smith
    Average 94 stars, based on 2304 article reviews
    Price from $9.99 to $1999.99
    glaxosmithkline gsk - by Bioz Stars, 2020-09
    94/100 stars
      Buy from Supplier

    92
    Pfizer Inc gsk
    Modified PRISMA (preferred reporting items for systematic reviews and meta-analyses) flowchart of search showing trials identified through literature search, trials requested from <t>GSK</t> CSDR.com, and those identified through GSK Study Register. CSDR.com=ClinicalStudyDataRequest.com; <t>GSK=GlaxoSmithKline</t>
    Gsk, supplied by Pfizer Inc, used in various techniques. Bioz Stars score: 92/100, based on 1585 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gsk/product/Pfizer Inc
    Average 92 stars, based on 1585 article reviews
    Price from $9.99 to $1999.99
    gsk - by Bioz Stars, 2020-09
    92/100 stars
      Buy from Supplier

    93
    Cell Signaling Technology Inc gsk 3β
    Silencing sCLU on effects of colony formation and cell proliferation A . the colony formation assay of HCC cells, and the representative micrographs from the Giemsa-stained colonies formed by HCCLM3 cells with transfection of shRNA-1, NC-shRNA or without transfection; B . the comparative analysis of the micrograph quantification results from the Giemsa-stained HCCLM3 cells transfected with shRNA-1, NC-shRNA or control group (n=3); C . the viability of HCCLM3 cells transfected with the shRNA-1, NC-shRNA or without transfection determined by CCK-8 assay on days 1 to 5; D . the levels of AKT, phosphorylated-AKT, <t>GSK-3β,</t> and phosphorylated-GSK-3β expressions detected by Western blotting. The data were presented as means ± SD (n=3). **, P
    Gsk 3β, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 2973 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gsk 3β/product/Cell Signaling Technology Inc
    Average 93 stars, based on 2973 article reviews
    Price from $9.99 to $1999.99
    gsk 3β - by Bioz Stars, 2020-09
    93/100 stars
      Buy from Supplier

    gsk  (Janssen)
    94
    Janssen gsk
    Silencing sCLU on effects of colony formation and cell proliferation A . the colony formation assay of HCC cells, and the representative micrographs from the Giemsa-stained colonies formed by HCCLM3 cells with transfection of shRNA-1, NC-shRNA or without transfection; B . the comparative analysis of the micrograph quantification results from the Giemsa-stained HCCLM3 cells transfected with shRNA-1, NC-shRNA or control group (n=3); C . the viability of HCCLM3 cells transfected with the shRNA-1, NC-shRNA or without transfection determined by CCK-8 assay on days 1 to 5; D . the levels of AKT, phosphorylated-AKT, <t>GSK-3β,</t> and phosphorylated-GSK-3β expressions detected by Western blotting. The data were presented as means ± SD (n=3). **, P
    Gsk, supplied by Janssen, used in various techniques. Bioz Stars score: 94/100, based on 813 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gsk/product/Janssen
    Average 94 stars, based on 813 article reviews
    Price from $9.99 to $1999.99
    gsk - by Bioz Stars, 2020-09
    94/100 stars
      Buy from Supplier

    94
    AstraZeneca gsk
    Silencing sCLU on effects of colony formation and cell proliferation A . the colony formation assay of HCC cells, and the representative micrographs from the Giemsa-stained colonies formed by HCCLM3 cells with transfection of shRNA-1, NC-shRNA or without transfection; B . the comparative analysis of the micrograph quantification results from the Giemsa-stained HCCLM3 cells transfected with shRNA-1, NC-shRNA or control group (n=3); C . the viability of HCCLM3 cells transfected with the shRNA-1, NC-shRNA or without transfection determined by CCK-8 assay on days 1 to 5; D . the levels of AKT, phosphorylated-AKT, <t>GSK-3β,</t> and phosphorylated-GSK-3β expressions detected by Western blotting. The data were presented as means ± SD (n=3). **, P
    Gsk, supplied by AstraZeneca, used in various techniques. Bioz Stars score: 94/100, based on 705 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gsk/product/AstraZeneca
    Average 94 stars, based on 705 article reviews
    Price from $9.99 to $1999.99
    gsk - by Bioz Stars, 2020-09
    94/100 stars
      Buy from Supplier

    gsk  (Sanofi)
    94
    Sanofi gsk
    Silencing sCLU on effects of colony formation and cell proliferation A . the colony formation assay of HCC cells, and the representative micrographs from the Giemsa-stained colonies formed by HCCLM3 cells with transfection of shRNA-1, NC-shRNA or without transfection; B . the comparative analysis of the micrograph quantification results from the Giemsa-stained HCCLM3 cells transfected with shRNA-1, NC-shRNA or control group (n=3); C . the viability of HCCLM3 cells transfected with the shRNA-1, NC-shRNA or without transfection determined by CCK-8 assay on days 1 to 5; D . the levels of AKT, phosphorylated-AKT, <t>GSK-3β,</t> and phosphorylated-GSK-3β expressions detected by Western blotting. The data were presented as means ± SD (n=3). **, P
    Gsk, supplied by Sanofi, used in various techniques. Bioz Stars score: 94/100, based on 516 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gsk/product/Sanofi
    Average 94 stars, based on 516 article reviews
    Price from $9.99 to $1999.99
    gsk - by Bioz Stars, 2020-09
    94/100 stars
      Buy from Supplier

    94
    Boehringer Ingelheim gsk
    Silencing sCLU on effects of colony formation and cell proliferation A . the colony formation assay of HCC cells, and the representative micrographs from the Giemsa-stained colonies formed by HCCLM3 cells with transfection of shRNA-1, NC-shRNA or without transfection; B . the comparative analysis of the micrograph quantification results from the Giemsa-stained HCCLM3 cells transfected with shRNA-1, NC-shRNA or control group (n=3); C . the viability of HCCLM3 cells transfected with the shRNA-1, NC-shRNA or without transfection determined by CCK-8 assay on days 1 to 5; D . the levels of AKT, phosphorylated-AKT, <t>GSK-3β,</t> and phosphorylated-GSK-3β expressions detected by Western blotting. The data were presented as means ± SD (n=3). **, P
    Gsk, supplied by Boehringer Ingelheim, used in various techniques. Bioz Stars score: 94/100, based on 458 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gsk/product/Boehringer Ingelheim
    Average 94 stars, based on 458 article reviews
    Price from $9.99 to $1999.99
    gsk - by Bioz Stars, 2020-09
    94/100 stars
      Buy from Supplier

    94
    Merck & Co gsk
    3.2. Variation in lots of <t>Oka/GSK</t> and <t>Oka/Merck</t>
    Gsk, supplied by Merck & Co, used in various techniques. Bioz Stars score: 94/100, based on 729 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gsk/product/Merck & Co
    Average 94 stars, based on 729 article reviews
    Price from $9.99 to $1999.99
    gsk - by Bioz Stars, 2020-09
    94/100 stars
      Buy from Supplier

    99
    Cell Signaling Technology Inc anti gsk 3β
    ( A ) Nuclear <t>GSK-3β</t> level in G AO compared to R AO SAMP8 mice. Level of GSK-3β decreased in the nuclear fraction of SAMP8 mice treated with G AO compared to that of SAMP8 mice treated with R AO. Data are represented as % control, and shown as mean ± SEM with *P
    Anti Gsk 3β, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 786 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti gsk 3β/product/Cell Signaling Technology Inc
    Average 99 stars, based on 786 article reviews
    Price from $9.99 to $1999.99
    anti gsk 3β - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    92
    Cell Signaling Technology Inc p gsk 3β
    Signaling events induced by FGF-23–Klotho. Immunoblotting showing that FGF-23 or Klotho alone have no effect on kinase activity in PTEC or FHs74Int cells. Combined effects of FGF-23 and Klotho show increased phosphorylation of ERK1/2, p38, JNK, AKT, IκB, and <t>GSK-3β.</t> α-Tubulin was used as a loading control.
    P Gsk 3β, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 92/100, based on 750 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/p gsk 3β/product/Cell Signaling Technology Inc
    Average 92 stars, based on 750 article reviews
    Price from $9.99 to $1999.99
    p gsk 3β - by Bioz Stars, 2020-09
    92/100 stars
      Buy from Supplier

    92
    Lundbeck gsk
    Signaling events induced by FGF-23–Klotho. Immunoblotting showing that FGF-23 or Klotho alone have no effect on kinase activity in PTEC or FHs74Int cells. Combined effects of FGF-23 and Klotho show increased phosphorylation of ERK1/2, p38, JNK, AKT, IκB, and <t>GSK-3β.</t> α-Tubulin was used as a loading control.
    Gsk, supplied by Lundbeck, used in various techniques. Bioz Stars score: 92/100, based on 261 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gsk/product/Lundbeck
    Average 92 stars, based on 261 article reviews
    Price from $9.99 to $1999.99
    gsk - by Bioz Stars, 2020-09
    92/100 stars
      Buy from Supplier

    gsk  (Teva)
    94
    Teva gsk
    Signaling events induced by FGF-23–Klotho. Immunoblotting showing that FGF-23 or Klotho alone have no effect on kinase activity in PTEC or FHs74Int cells. Combined effects of FGF-23 and Klotho show increased phosphorylation of ERK1/2, p38, JNK, AKT, IκB, and <t>GSK-3β.</t> α-Tubulin was used as a loading control.
    Gsk, supplied by Teva, used in various techniques. Bioz Stars score: 94/100, based on 171 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gsk/product/Teva
    Average 94 stars, based on 171 article reviews
    Price from $9.99 to $1999.99
    gsk - by Bioz Stars, 2020-09
    94/100 stars
      Buy from Supplier

    92
    Medtronic gsk
    Signaling events induced by FGF-23–Klotho. Immunoblotting showing that FGF-23 or Klotho alone have no effect on kinase activity in PTEC or FHs74Int cells. Combined effects of FGF-23 and Klotho show increased phosphorylation of ERK1/2, p38, JNK, AKT, IκB, and <t>GSK-3β.</t> α-Tubulin was used as a loading control.
    Gsk, supplied by Medtronic, used in various techniques. Bioz Stars score: 92/100, based on 154 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gsk/product/Medtronic
    Average 92 stars, based on 154 article reviews
    Price from $9.99 to $1999.99
    gsk - by Bioz Stars, 2020-09
    92/100 stars
      Buy from Supplier

    gsk  (Takeda)
    94
    Takeda gsk
    Signaling events induced by FGF-23–Klotho. Immunoblotting showing that FGF-23 or Klotho alone have no effect on kinase activity in PTEC or FHs74Int cells. Combined effects of FGF-23 and Klotho show increased phosphorylation of ERK1/2, p38, JNK, AKT, IκB, and <t>GSK-3β.</t> α-Tubulin was used as a loading control.
    Gsk, supplied by Takeda, used in various techniques. Bioz Stars score: 94/100, based on 185 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gsk/product/Takeda
    Average 94 stars, based on 185 article reviews
    Price from $9.99 to $1999.99
    gsk - by Bioz Stars, 2020-09
    94/100 stars
      Buy from Supplier

    gsk  (Chiesi)
    92
    Chiesi gsk
    Signaling events induced by FGF-23–Klotho. Immunoblotting showing that FGF-23 or Klotho alone have no effect on kinase activity in PTEC or FHs74Int cells. Combined effects of FGF-23 and Klotho show increased phosphorylation of ERK1/2, p38, JNK, AKT, IκB, and <t>GSK-3β.</t> α-Tubulin was used as a loading control.
    Gsk, supplied by Chiesi, used in various techniques. Bioz Stars score: 92/100, based on 165 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gsk/product/Chiesi
    Average 92 stars, based on 165 article reviews
    Price from $9.99 to $1999.99
    gsk - by Bioz Stars, 2020-09
    92/100 stars
      Buy from Supplier

    gsk  (Amgen)
    92
    Amgen gsk
    Signaling events induced by FGF-23–Klotho. Immunoblotting showing that FGF-23 or Klotho alone have no effect on kinase activity in PTEC or FHs74Int cells. Combined effects of FGF-23 and Klotho show increased phosphorylation of ERK1/2, p38, JNK, AKT, IκB, and <t>GSK-3β.</t> α-Tubulin was used as a loading control.
    Gsk, supplied by Amgen, used in various techniques. Bioz Stars score: 92/100, based on 225 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gsk/product/Amgen
    Average 92 stars, based on 225 article reviews
    Price from $9.99 to $1999.99
    gsk - by Bioz Stars, 2020-09
    92/100 stars
      Buy from Supplier

    99
    Cell Signaling Technology Inc phospho gsk 3β ser9
    (A) Lysate from Ad-FAK-HelixScr and Ad-FAK-Helix infected cells exposed to ambient (A) or 15 mmHg (P) while in suspension, or allowed to adhere to collagen I (Adh) were probed for phospho- (top) and total (bottom) FAK, Akt1, and GSK3B. Blots were cut at the level of 75 kD,, and the higher weight bands were incubated with pFAK/FAK (125 kDa) antibodies while the lower weight bands received pAkt1/Akt1 (60 kDa) and pGSK3B/GSK3B (46 kDa) probes. (B) In suspended cells, pFAK increased following 15 mmHG exposure vs. exposure to ambient atmosphere in Ad-FAK-HelixScr, but not Ad-FAK-Helix infected cells. In contrast, adhesion increased FAK phosphorylation over suspended cells at ambient pressure in both Ad-FAK-HelixScr and Ad-FAK-Helix infected cells. (C) Both Ad-FAK-HelixScr and Ad-FAK-Helix virus infected cells exhibited increased Akt1 phosphorylation after exposure to 15 mmHg pressure as well as after adhesion. (D) <t>GSK-3β</t> phosphorylation also increased in both the virus treated cells in response to adhesion; however, in pressure treated groups, GSK-3β phosphorylation decreased in the Ad-FAK-HelixScr but not the Ad-FAK-Helix virus treated cells (n = 4-8, * p
    Phospho Gsk 3β Ser9, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 477 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phospho gsk 3β ser9/product/Cell Signaling Technology Inc
    Average 99 stars, based on 477 article reviews
    Price from $9.99 to $1999.99
    phospho gsk 3β ser9 - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    93
    Genentech gsk
    (A) Lysate from Ad-FAK-HelixScr and Ad-FAK-Helix infected cells exposed to ambient (A) or 15 mmHg (P) while in suspension, or allowed to adhere to collagen I (Adh) were probed for phospho- (top) and total (bottom) FAK, Akt1, and GSK3B. Blots were cut at the level of 75 kD,, and the higher weight bands were incubated with pFAK/FAK (125 kDa) antibodies while the lower weight bands received pAkt1/Akt1 (60 kDa) and pGSK3B/GSK3B (46 kDa) probes. (B) In suspended cells, pFAK increased following 15 mmHG exposure vs. exposure to ambient atmosphere in Ad-FAK-HelixScr, but not Ad-FAK-Helix infected cells. In contrast, adhesion increased FAK phosphorylation over suspended cells at ambient pressure in both Ad-FAK-HelixScr and Ad-FAK-Helix infected cells. (C) Both Ad-FAK-HelixScr and Ad-FAK-Helix virus infected cells exhibited increased Akt1 phosphorylation after exposure to 15 mmHg pressure as well as after adhesion. (D) <t>GSK-3β</t> phosphorylation also increased in both the virus treated cells in response to adhesion; however, in pressure treated groups, GSK-3β phosphorylation decreased in the Ad-FAK-HelixScr but not the Ad-FAK-Helix virus treated cells (n = 4-8, * p
    Gsk, supplied by Genentech, used in various techniques. Bioz Stars score: 93/100, based on 204 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gsk/product/Genentech
    Average 93 stars, based on 204 article reviews
    Price from $9.99 to $1999.99
    gsk - by Bioz Stars, 2020-09
    93/100 stars
      Buy from Supplier

    99
    Cell Signaling Technology Inc p gsk3β s9
    Akt activation is necessary for the enhanced transformation caused by LCMT-1 knockdown. (A) Lysates from VC and L3 cells stably expressing an empty control plasmid (VC-control and L3-control, respectively) and lysates from L3 cells stably expressing dominant-negative Akt-AA (L3-Akt-AA) were analyzed by Western blot analysis for the expression of Akt-AA, LCMT-1, and GAPDH. (B) knockdownknockdownAnchorage-independent growth of VC-control, L3-control, and L3-Akt-AA cells in soft agar. Photographs show small, single, representative fields within the agars. Average colony numbers (C) and average colony volumes (D) were determined, and data are shown in graphs as fold change relative to VC-control. Error bars represent SD of three independent experiments performed in triplicate. (E) Lysates from VC-control, L3-control, and L3-Akt-AA suspension colonies were analyzed by Western blot analysis for changes in activation of endogenous Akt. GAPDH was used as a loading control. (F) Graph depicts the average fold change in the levels of phospho-Akt T308, phospho-Akt S473, and <t>phospho-GSK3β</t> S9 in three immunoblot experiments relative to VC-control. (G) Probing of VC-control, L3-control, and L3-Akt-AA lysates with phospho-Akt substrate antibody shows that dominant-negative Akt expression prevents the increased phosphorylation of many proteins caused by LCMT-1 knockdown (arrows show examples). The bracket with asterisk indicates phospho-rpS6, which ran at the bottom of this 7.5% SDS-PAGE gel. (H and I) Lysates were probed for changes in p70 and p85 S6K activation and rpS6 phosphorylation. Total p70 and p85 S6K and total rpS6 were used as controls. (J) Graph depicts the average fold change in the levels of phospho-p85, phospho-p70, and phospho-rpS6 in three immunoblot experiments relative to VC-control. Error bars represent SD of three independent experiments. For all graphs: * P ≤ .05. ** P ≤ .01.
    P Gsk3β S9, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 79 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/p gsk3β s9/product/Cell Signaling Technology Inc
    Average 99 stars, based on 79 article reviews
    Price from $9.99 to $1999.99
    p gsk3β s9 - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    gsk  (AbbVie)
    92
    AbbVie gsk
    Akt activation is necessary for the enhanced transformation caused by LCMT-1 knockdown. (A) Lysates from VC and L3 cells stably expressing an empty control plasmid (VC-control and L3-control, respectively) and lysates from L3 cells stably expressing dominant-negative Akt-AA (L3-Akt-AA) were analyzed by Western blot analysis for the expression of Akt-AA, LCMT-1, and GAPDH. (B) knockdownknockdownAnchorage-independent growth of VC-control, L3-control, and L3-Akt-AA cells in soft agar. Photographs show small, single, representative fields within the agars. Average colony numbers (C) and average colony volumes (D) were determined, and data are shown in graphs as fold change relative to VC-control. Error bars represent SD of three independent experiments performed in triplicate. (E) Lysates from VC-control, L3-control, and L3-Akt-AA suspension colonies were analyzed by Western blot analysis for changes in activation of endogenous Akt. GAPDH was used as a loading control. (F) Graph depicts the average fold change in the levels of phospho-Akt T308, phospho-Akt S473, and <t>phospho-GSK3β</t> S9 in three immunoblot experiments relative to VC-control. (G) Probing of VC-control, L3-control, and L3-Akt-AA lysates with phospho-Akt substrate antibody shows that dominant-negative Akt expression prevents the increased phosphorylation of many proteins caused by LCMT-1 knockdown (arrows show examples). The bracket with asterisk indicates phospho-rpS6, which ran at the bottom of this 7.5% SDS-PAGE gel. (H and I) Lysates were probed for changes in p70 and p85 S6K activation and rpS6 phosphorylation. Total p70 and p85 S6K and total rpS6 were used as controls. (J) Graph depicts the average fold change in the levels of phospho-p85, phospho-p70, and phospho-rpS6 in three immunoblot experiments relative to VC-control. Error bars represent SD of three independent experiments. For all graphs: * P ≤ .05. ** P ≤ .01.
    Gsk, supplied by AbbVie, used in various techniques. Bioz Stars score: 92/100, based on 93 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gsk/product/AbbVie
    Average 92 stars, based on 93 article reviews
    Price from $9.99 to $1999.99
    gsk - by Bioz Stars, 2020-09
    92/100 stars
      Buy from Supplier

    93
    Eli Lilly gsk
    Akt activation is necessary for the enhanced transformation caused by LCMT-1 knockdown. (A) Lysates from VC and L3 cells stably expressing an empty control plasmid (VC-control and L3-control, respectively) and lysates from L3 cells stably expressing dominant-negative Akt-AA (L3-Akt-AA) were analyzed by Western blot analysis for the expression of Akt-AA, LCMT-1, and GAPDH. (B) knockdownknockdownAnchorage-independent growth of VC-control, L3-control, and L3-Akt-AA cells in soft agar. Photographs show small, single, representative fields within the agars. Average colony numbers (C) and average colony volumes (D) were determined, and data are shown in graphs as fold change relative to VC-control. Error bars represent SD of three independent experiments performed in triplicate. (E) Lysates from VC-control, L3-control, and L3-Akt-AA suspension colonies were analyzed by Western blot analysis for changes in activation of endogenous Akt. GAPDH was used as a loading control. (F) Graph depicts the average fold change in the levels of phospho-Akt T308, phospho-Akt S473, and <t>phospho-GSK3β</t> S9 in three immunoblot experiments relative to VC-control. (G) Probing of VC-control, L3-control, and L3-Akt-AA lysates with phospho-Akt substrate antibody shows that dominant-negative Akt expression prevents the increased phosphorylation of many proteins caused by LCMT-1 knockdown (arrows show examples). The bracket with asterisk indicates phospho-rpS6, which ran at the bottom of this 7.5% SDS-PAGE gel. (H and I) Lysates were probed for changes in p70 and p85 S6K activation and rpS6 phosphorylation. Total p70 and p85 S6K and total rpS6 were used as controls. (J) Graph depicts the average fold change in the levels of phospho-p85, phospho-p70, and phospho-rpS6 in three immunoblot experiments relative to VC-control. Error bars represent SD of three independent experiments. For all graphs: * P ≤ .05. ** P ≤ .01.
    Gsk, supplied by Eli Lilly, used in various techniques. Bioz Stars score: 93/100, based on 164 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gsk/product/Eli Lilly
    Average 93 stars, based on 164 article reviews
    Price from $9.99 to $1999.99
    gsk - by Bioz Stars, 2020-09
    93/100 stars
      Buy from Supplier

    Image Search Results


    Principal Component Analysis plots. Chemical diversity of the GSK, Novartis and St Jude libraries displayed (Panel A); Overlap in chemical diversity of the combined datasets and the commercially available compounds (Panel B); Overlap in chemical diversity of the commercially available compounds where the drug-like and probe-like chemotypes were annotated (Panel C).

    Journal: PLoS ONE

    Article Title: The Open Access Malaria Box: A Drug Discovery Catalyst for Neglected Diseases

    doi: 10.1371/journal.pone.0062906

    Figure Lengend Snippet: Principal Component Analysis plots. Chemical diversity of the GSK, Novartis and St Jude libraries displayed (Panel A); Overlap in chemical diversity of the combined datasets and the commercially available compounds (Panel B); Overlap in chemical diversity of the commercially available compounds where the drug-like and probe-like chemotypes were annotated (Panel C).

    Article Snippet: Materials and Methods The data reported by GSK , Novartis , St. Jude Children's Research Hospital constitute the initial set of compounds used in this effort.

    Techniques:

    Venn diagram presenting the overlap of structures in the St. Jude, Novartis and GSK datasets. The data was generated using Pipeline Pilot 8.5, and displayed using R 2.14.1.

    Journal: PLoS ONE

    Article Title: The Open Access Malaria Box: A Drug Discovery Catalyst for Neglected Diseases

    doi: 10.1371/journal.pone.0062906

    Figure Lengend Snippet: Venn diagram presenting the overlap of structures in the St. Jude, Novartis and GSK datasets. The data was generated using Pipeline Pilot 8.5, and displayed using R 2.14.1.

    Article Snippet: Materials and Methods The data reported by GSK , Novartis , St. Jude Children's Research Hospital constitute the initial set of compounds used in this effort.

    Techniques: Generated

    St Jude's, Novartis and GSK datasets profiled with respect to molecular weight, the number of hydrogen-bond donors, ALogP and N+O (nitrogen count plus oxygen count).

    Journal: PLoS ONE

    Article Title: The Open Access Malaria Box: A Drug Discovery Catalyst for Neglected Diseases

    doi: 10.1371/journal.pone.0062906

    Figure Lengend Snippet: St Jude's, Novartis and GSK datasets profiled with respect to molecular weight, the number of hydrogen-bond donors, ALogP and N+O (nitrogen count plus oxygen count).

    Article Snippet: Materials and Methods The data reported by GSK , Novartis , St. Jude Children's Research Hospital constitute the initial set of compounds used in this effort.

    Techniques: Molecular Weight

    Modified PRISMA (preferred reporting items for systematic reviews and meta-analyses) flowchart of search showing trials identified through literature search, trials requested from GSK CSDR.com, and those identified through GSK Study Register. CSDR.com=ClinicalStudyDataRequest.com; GSK=GlaxoSmithKline

    Journal: The BMJ

    Article Title: Updating insights into rosiglitazone and cardiovascular risk through shared data: individual patient and summary level meta-analyses

    doi: 10.1136/bmj.l7078

    Figure Lengend Snippet: Modified PRISMA (preferred reporting items for systematic reviews and meta-analyses) flowchart of search showing trials identified through literature search, trials requested from GSK CSDR.com, and those identified through GSK Study Register. CSDR.com=ClinicalStudyDataRequest.com; GSK=GlaxoSmithKline

    Article Snippet: Introduction Rosiglitazone is manufactured by GlaxoSmithKline (GSK) under the brand name Avandia.

    Techniques: Modification

    Modified PRISMA (preferred reporting items for systematic reviews and meta-analyses) flowchart of search showing trials identified in previous meta-analyses and on ClinicalTrials.gov. CSDR.com=ClinicalStudyDataRequest.com; GSK=GlaxoSmithKline

    Journal: The BMJ

    Article Title: Updating insights into rosiglitazone and cardiovascular risk through shared data: individual patient and summary level meta-analyses

    doi: 10.1136/bmj.l7078

    Figure Lengend Snippet: Modified PRISMA (preferred reporting items for systematic reviews and meta-analyses) flowchart of search showing trials identified in previous meta-analyses and on ClinicalTrials.gov. CSDR.com=ClinicalStudyDataRequest.com; GSK=GlaxoSmithKline

    Article Snippet: Introduction Rosiglitazone is manufactured by GlaxoSmithKline (GSK) under the brand name Avandia.

    Techniques: Modification

    Silencing sCLU on effects of colony formation and cell proliferation A . the colony formation assay of HCC cells, and the representative micrographs from the Giemsa-stained colonies formed by HCCLM3 cells with transfection of shRNA-1, NC-shRNA or without transfection; B . the comparative analysis of the micrograph quantification results from the Giemsa-stained HCCLM3 cells transfected with shRNA-1, NC-shRNA or control group (n=3); C . the viability of HCCLM3 cells transfected with the shRNA-1, NC-shRNA or without transfection determined by CCK-8 assay on days 1 to 5; D . the levels of AKT, phosphorylated-AKT, GSK-3β, and phosphorylated-GSK-3β expressions detected by Western blotting. The data were presented as means ± SD (n=3). **, P

    Journal: Oncotarget

    Article Title: Oncogenic secretory clusterin in hepatocellular carcinoma: Expression at early staging and emerging molecular target

    doi: 10.18632/oncotarget.13674

    Figure Lengend Snippet: Silencing sCLU on effects of colony formation and cell proliferation A . the colony formation assay of HCC cells, and the representative micrographs from the Giemsa-stained colonies formed by HCCLM3 cells with transfection of shRNA-1, NC-shRNA or without transfection; B . the comparative analysis of the micrograph quantification results from the Giemsa-stained HCCLM3 cells transfected with shRNA-1, NC-shRNA or control group (n=3); C . the viability of HCCLM3 cells transfected with the shRNA-1, NC-shRNA or without transfection determined by CCK-8 assay on days 1 to 5; D . the levels of AKT, phosphorylated-AKT, GSK-3β, and phosphorylated-GSK-3β expressions detected by Western blotting. The data were presented as means ± SD (n=3). **, P

    Article Snippet: Antibodies were diluted as follows: GAPDH, AKT and GSK-3β (1:1000; Cell Signaling, USA); sCLU (1:500; Santa Cruz, USA); phos-AKT and phos-GSK-3β (1:1000; Abcam, USA); IgG horseradish peroxidase conjugate (1:1000; Univ-bio, Nanjing, China).

    Techniques: Colony Assay, Staining, Transfection, shRNA, CCK-8 Assay, Western Blot

    The effects of hUCB-MSCs on cell viability, ALP activity, and AKT/GSK3β/β-catenin pathway regulation of hDPCs. hDPCs were co-cultured with hUCB-MSCs for 48 h or 120 h in two types of culture media (DMEM and MEM). (A) The cell viability was estimated using a WST-8 assay. (B) ALP activity. (C) Representative images of Western blot protein assays for p-AKT, AKT, p-GSK3β, GSK3β, β-catenin, and proliferating cell nuclear antigen (PCNA) in dermal papilla cells. hDPCs and hUCB-MSCs were seeded in 6-well transwell plates. After 48 h, the cell lysates were harvested for Western blot assays. (D) Intensities of the immunoreactive bands on the Western blots as quantified by densitometric analysis. For all graphs, the data is reported as mean±SD. * p

    Journal: The Korean Journal of Physiology & Pharmacology : Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology

    Article Title: Human umbilical cord blood mesenchymal stem cells engineered to overexpress growth factors accelerate outcomes in hair growth

    doi: 10.4196/kjpp.2018.22.5.555

    Figure Lengend Snippet: The effects of hUCB-MSCs on cell viability, ALP activity, and AKT/GSK3β/β-catenin pathway regulation of hDPCs. hDPCs were co-cultured with hUCB-MSCs for 48 h or 120 h in two types of culture media (DMEM and MEM). (A) The cell viability was estimated using a WST-8 assay. (B) ALP activity. (C) Representative images of Western blot protein assays for p-AKT, AKT, p-GSK3β, GSK3β, β-catenin, and proliferating cell nuclear antigen (PCNA) in dermal papilla cells. hDPCs and hUCB-MSCs were seeded in 6-well transwell plates. After 48 h, the cell lysates were harvested for Western blot assays. (D) Intensities of the immunoreactive bands on the Western blots as quantified by densitometric analysis. For all graphs, the data is reported as mean±SD. * p

    Article Snippet: Antibodies for phosphorylated-AKT (1:1000, cat. no. #9271, Cell Signaling Technology, Inc., Danvers, MA, USA), AKT (1:1000, cat. no. #4685, Cell Signaling Technology, Inc.), phopholyrated-GSK3β (1:1000, cat. no. #9323, Cell Signaling Technology, Inc), GSK3β (1:1000, cat. no. #12456, Cell Signaling Technology, Inc.), β-catenin (1:1000, cat. no. 610153, BD Biosciences, Franklin Lakes, NJ, USA), proliferating cell nuclear antigen (1:1000, cat. no. #13110, Cell Signaling Technology, Inc.), β-actin (1:1000, cat. no. sc-4778, Santa Cruz Biotechnology, Inc., Dallas, TX, USA), ALP (1:100, cat. no. SC-15065, Santa Cruz), CD 133 (cat. no. NB120-16518, Novus Biologicals, CO, USA) were used as the primary antibodies, and goat anti-rabbit (cat. no. BA-1000, VECTOR LABORATORIES, INC.) and goat anti-mouse (HRP) (cat. no. BA-9200, VECTOR LABORATORIES, INC.) were used as the secondary antibodies (1:10000).

    Techniques: ALP Assay, Activity Assay, Cell Culture, Western Blot

    3.2. Variation in lots of Oka/GSK and Oka/Merck

    Journal: Vaccine

    Article Title: Population diversity in batches of the varicella Oka vaccine

    doi: 10.1016/j.vaccine.2011.02.021

    Figure Lengend Snippet: 3.2. Variation in lots of Oka/GSK and Oka/Merck

    Article Snippet: Five lots each of GSK and Merck vaccines derived in each case from three batches were available for analysis ( ).

    Techniques:

    Comparison of range and average allele frequencies at polymorphic sites in Oka/Merck (black) and Oka/GSK (grey) vaccines. The whiskers on the box plots represent the full range of estimates of the wild-type allele, from the three batches obtained from

    Journal: Vaccine

    Article Title: Population diversity in batches of the varicella Oka vaccine

    doi: 10.1016/j.vaccine.2011.02.021

    Figure Lengend Snippet: Comparison of range and average allele frequencies at polymorphic sites in Oka/Merck (black) and Oka/GSK (grey) vaccines. The whiskers on the box plots represent the full range of estimates of the wild-type allele, from the three batches obtained from

    Article Snippet: Five lots each of GSK and Merck vaccines derived in each case from three batches were available for analysis ( ).

    Techniques:

    ( A ) Nuclear GSK-3β level in G AO compared to R AO SAMP8 mice. Level of GSK-3β decreased in the nuclear fraction of SAMP8 mice treated with G AO compared to that of SAMP8 mice treated with R AO. Data are represented as % control, and shown as mean ± SEM with *P

    Journal: Free radical biology & medicine

    Article Title: Antisense Oligonucleotide Against GSK-3β in Brain of SAMP8 Mice Improves Learning and Memory and Decreases Oxidative Stress: Involvement of Transcription Factor Nrf2 and Implications for Alzheimer Disease

    doi: 10.1016/j.freeradbiomed.2013.11.014

    Figure Lengend Snippet: ( A ) Nuclear GSK-3β level in G AO compared to R AO SAMP8 mice. Level of GSK-3β decreased in the nuclear fraction of SAMP8 mice treated with G AO compared to that of SAMP8 mice treated with R AO. Data are represented as % control, and shown as mean ± SEM with *P

    Article Snippet: The protein transfer from the gel to the membrane was checked using the reversible protein stain, ponceau S. The subsequent protein-bound membranes were incubated for 90 min in fresh blocking buffer, and then incubated for 3 h in dilutions of primary anti-GSK-3β (rabbit, Cell Signaling, Danvers, MA, USA, dilution 1:2000), anti-Nrf2 (rabbit, Enzo Life Sciences, Farmingdale, NY, USA, dilution 1:1000), AT180 (Pierce, Rockford, IL, USA, dilution 1:1000) and anti-GST (rabbit, Epitomics, Burlingame, CA, USA, dilution 1:1000) prepared in fresh wash blot.

    Techniques: Mouse Assay

    ( A ) Protein carbonyl level in G AO compared to R AO SAMP8 mice. Protein Carbonyl level decreased in SAMP8 mice treated with AO directed at GSK-3β (N=9) compared to that of SAMP8 mice treated with random AO (N=7). Data are represented as % control, and shown as mean ± SEM with *P

    Journal: Free radical biology & medicine

    Article Title: Antisense Oligonucleotide Against GSK-3β in Brain of SAMP8 Mice Improves Learning and Memory and Decreases Oxidative Stress: Involvement of Transcription Factor Nrf2 and Implications for Alzheimer Disease

    doi: 10.1016/j.freeradbiomed.2013.11.014

    Figure Lengend Snippet: ( A ) Protein carbonyl level in G AO compared to R AO SAMP8 mice. Protein Carbonyl level decreased in SAMP8 mice treated with AO directed at GSK-3β (N=9) compared to that of SAMP8 mice treated with random AO (N=7). Data are represented as % control, and shown as mean ± SEM with *P

    Article Snippet: The protein transfer from the gel to the membrane was checked using the reversible protein stain, ponceau S. The subsequent protein-bound membranes were incubated for 90 min in fresh blocking buffer, and then incubated for 3 h in dilutions of primary anti-GSK-3β (rabbit, Cell Signaling, Danvers, MA, USA, dilution 1:2000), anti-Nrf2 (rabbit, Enzo Life Sciences, Farmingdale, NY, USA, dilution 1:1000), AT180 (Pierce, Rockford, IL, USA, dilution 1:1000) and anti-GST (rabbit, Epitomics, Burlingame, CA, USA, dilution 1:1000) prepared in fresh wash blot.

    Techniques: Mouse Assay

    Signaling events induced by FGF-23–Klotho. Immunoblotting showing that FGF-23 or Klotho alone have no effect on kinase activity in PTEC or FHs74Int cells. Combined effects of FGF-23 and Klotho show increased phosphorylation of ERK1/2, p38, JNK, AKT, IκB, and GSK-3β. α-Tubulin was used as a loading control.

    Journal: The Journal of Cell Biology

    Article Title: FGF-23-Klotho signaling stimulates proliferation and prevents vitamin D-induced apoptosis

    doi: 10.1083/jcb.200803024

    Figure Lengend Snippet: Signaling events induced by FGF-23–Klotho. Immunoblotting showing that FGF-23 or Klotho alone have no effect on kinase activity in PTEC or FHs74Int cells. Combined effects of FGF-23 and Klotho show increased phosphorylation of ERK1/2, p38, JNK, AKT, IκB, and GSK-3β. α-Tubulin was used as a loading control.

    Article Snippet: P-AKT, P-GSK-3β (Cell Signaling Technology), Cyclin D1, c-myc, and CYP27B1 (Santa Cruz Biotechnology, Inc.) antibodies were conjugated to various Bio-Plex carboxylated beads (with unique optical codes) using the Bio-Plex Amine Coupling kit (Bio-Rad Laboratories). α-Tubulin antibody (EMD) was also conjugated to Bio-Plex carboxylated beads to be used as an internal control.

    Techniques: Activity Assay

    Western blot showing glycogen synthase kinase-3β (GSK-3β) expression in LV from the experimental groups. Note the enhancement in GSK-3β phosphorylation with 5/6 Nx and its attenuation by treatment with curcumin and enalapril. The summary results are shown as the ratio of total GSK-3β/glyceraldehyde-3-phosphate dehydrogenase (GAPDH), phosphorylated (p) GSK-3β/GAPDH, and pGSK-3β/GSK-3β.

    Journal: American Journal of Physiology - Heart and Circulatory Physiology

    Article Title: Curcumin prevents cardiac remodeling secondary to chronic renal failure through deactivation of hypertrophic signaling in rats

    doi: 10.1152/ajpheart.00154.2010

    Figure Lengend Snippet: Western blot showing glycogen synthase kinase-3β (GSK-3β) expression in LV from the experimental groups. Note the enhancement in GSK-3β phosphorylation with 5/6 Nx and its attenuation by treatment with curcumin and enalapril. The summary results are shown as the ratio of total GSK-3β/glyceraldehyde-3-phosphate dehydrogenase (GAPDH), phosphorylated (p) GSK-3β/GAPDH, and pGSK-3β/GSK-3β.

    Article Snippet: Phospho-GSK-3β was purchased from Cell Signaling (Cell Signaling Technology, Danvers, MA).

    Techniques: Western Blot, Expressing

    Effects of antenatal antioxidant on GSK3β gene expression and its activity in the hearts of adult offspring. Hearts were isolated from adult offspring that were prenatally exposed to saline control or nicotine along without or with NAC treatment. Phospho-GSK3β ( A ) and total GSK3β protein ( B ) levels in the hearts were determined by Western blots. The protein density was normalized to GAPDH. All of the data expressed as means ± SEM of animal numbers per group. Data were analyzed by Student’s t -test. *P

    Journal: PLoS ONE

    Article Title: Protective Effect of Antenatal Antioxidant on Nicotine-Induced Heart Ischemia-Sensitive Phenotype in Rat Offspring

    doi: 10.1371/journal.pone.0150557

    Figure Lengend Snippet: Effects of antenatal antioxidant on GSK3β gene expression and its activity in the hearts of adult offspring. Hearts were isolated from adult offspring that were prenatally exposed to saline control or nicotine along without or with NAC treatment. Phospho-GSK3β ( A ) and total GSK3β protein ( B ) levels in the hearts were determined by Western blots. The protein density was normalized to GAPDH. All of the data expressed as means ± SEM of animal numbers per group. Data were analyzed by Student’s t -test. *P

    Article Snippet: Proteins were then transferred to the nitrocellulose membrane and incubated with primary antibodies for PKCε (Santa Cruz Biotechnology, Inc., Santa Cruz, CA), phospho-GSK3β (Ser 9), and total GSK3β (Cell Signaling, Danvers, MA), respectively.

    Techniques: Expressing, Activity Assay, Isolation, Western Blot

    (A) Lysate from Ad-FAK-HelixScr and Ad-FAK-Helix infected cells exposed to ambient (A) or 15 mmHg (P) while in suspension, or allowed to adhere to collagen I (Adh) were probed for phospho- (top) and total (bottom) FAK, Akt1, and GSK3B. Blots were cut at the level of 75 kD,, and the higher weight bands were incubated with pFAK/FAK (125 kDa) antibodies while the lower weight bands received pAkt1/Akt1 (60 kDa) and pGSK3B/GSK3B (46 kDa) probes. (B) In suspended cells, pFAK increased following 15 mmHG exposure vs. exposure to ambient atmosphere in Ad-FAK-HelixScr, but not Ad-FAK-Helix infected cells. In contrast, adhesion increased FAK phosphorylation over suspended cells at ambient pressure in both Ad-FAK-HelixScr and Ad-FAK-Helix infected cells. (C) Both Ad-FAK-HelixScr and Ad-FAK-Helix virus infected cells exhibited increased Akt1 phosphorylation after exposure to 15 mmHg pressure as well as after adhesion. (D) GSK-3β phosphorylation also increased in both the virus treated cells in response to adhesion; however, in pressure treated groups, GSK-3β phosphorylation decreased in the Ad-FAK-HelixScr but not the Ad-FAK-Helix virus treated cells (n = 4-8, * p

    Journal: Oncotarget

    Article Title: Inhibition of pressure-activated cancer cell adhesion by FAK-derived peptides

    doi: 10.18632/oncotarget.20556

    Figure Lengend Snippet: (A) Lysate from Ad-FAK-HelixScr and Ad-FAK-Helix infected cells exposed to ambient (A) or 15 mmHg (P) while in suspension, or allowed to adhere to collagen I (Adh) were probed for phospho- (top) and total (bottom) FAK, Akt1, and GSK3B. Blots were cut at the level of 75 kD,, and the higher weight bands were incubated with pFAK/FAK (125 kDa) antibodies while the lower weight bands received pAkt1/Akt1 (60 kDa) and pGSK3B/GSK3B (46 kDa) probes. (B) In suspended cells, pFAK increased following 15 mmHG exposure vs. exposure to ambient atmosphere in Ad-FAK-HelixScr, but not Ad-FAK-Helix infected cells. In contrast, adhesion increased FAK phosphorylation over suspended cells at ambient pressure in both Ad-FAK-HelixScr and Ad-FAK-Helix infected cells. (C) Both Ad-FAK-HelixScr and Ad-FAK-Helix virus infected cells exhibited increased Akt1 phosphorylation after exposure to 15 mmHg pressure as well as after adhesion. (D) GSK-3β phosphorylation also increased in both the virus treated cells in response to adhesion; however, in pressure treated groups, GSK-3β phosphorylation decreased in the Ad-FAK-HelixScr but not the Ad-FAK-Helix virus treated cells (n = 4-8, * p

    Article Snippet: Membranes were blocked for 1 hour at room temperature with Odyssey TBS Blocking Buffer (Amersham Life Science, Arlington Heights, IL) and blotted overnight at 4°C with antibodies against FAK (#3285), Phospho-FAK Tyr397 (#3283), Akt1 (#2967), Phospho-Akt1 Ser473 (#9271), GSK-3β (#9315), Phospho-GSK-3β Ser9 (#9315), or the GST tag (#2624) (CST, Beverly, MA).

    Techniques: Infection, Incubation

    Akt activation is necessary for the enhanced transformation caused by LCMT-1 knockdown. (A) Lysates from VC and L3 cells stably expressing an empty control plasmid (VC-control and L3-control, respectively) and lysates from L3 cells stably expressing dominant-negative Akt-AA (L3-Akt-AA) were analyzed by Western blot analysis for the expression of Akt-AA, LCMT-1, and GAPDH. (B) knockdownknockdownAnchorage-independent growth of VC-control, L3-control, and L3-Akt-AA cells in soft agar. Photographs show small, single, representative fields within the agars. Average colony numbers (C) and average colony volumes (D) were determined, and data are shown in graphs as fold change relative to VC-control. Error bars represent SD of three independent experiments performed in triplicate. (E) Lysates from VC-control, L3-control, and L3-Akt-AA suspension colonies were analyzed by Western blot analysis for changes in activation of endogenous Akt. GAPDH was used as a loading control. (F) Graph depicts the average fold change in the levels of phospho-Akt T308, phospho-Akt S473, and phospho-GSK3β S9 in three immunoblot experiments relative to VC-control. (G) Probing of VC-control, L3-control, and L3-Akt-AA lysates with phospho-Akt substrate antibody shows that dominant-negative Akt expression prevents the increased phosphorylation of many proteins caused by LCMT-1 knockdown (arrows show examples). The bracket with asterisk indicates phospho-rpS6, which ran at the bottom of this 7.5% SDS-PAGE gel. (H and I) Lysates were probed for changes in p70 and p85 S6K activation and rpS6 phosphorylation. Total p70 and p85 S6K and total rpS6 were used as controls. (J) Graph depicts the average fold change in the levels of phospho-p85, phospho-p70, and phospho-rpS6 in three immunoblot experiments relative to VC-control. Error bars represent SD of three independent experiments. For all graphs: * P ≤ .05. ** P ≤ .01.

    Journal: Neoplasia (New York, N.Y.)

    Article Title: Circumventing Cellular Control of PP2A by Methylation Promotes Transformation in an Akt-Dependent Manner 1

    doi:

    Figure Lengend Snippet: Akt activation is necessary for the enhanced transformation caused by LCMT-1 knockdown. (A) Lysates from VC and L3 cells stably expressing an empty control plasmid (VC-control and L3-control, respectively) and lysates from L3 cells stably expressing dominant-negative Akt-AA (L3-Akt-AA) were analyzed by Western blot analysis for the expression of Akt-AA, LCMT-1, and GAPDH. (B) knockdownknockdownAnchorage-independent growth of VC-control, L3-control, and L3-Akt-AA cells in soft agar. Photographs show small, single, representative fields within the agars. Average colony numbers (C) and average colony volumes (D) were determined, and data are shown in graphs as fold change relative to VC-control. Error bars represent SD of three independent experiments performed in triplicate. (E) Lysates from VC-control, L3-control, and L3-Akt-AA suspension colonies were analyzed by Western blot analysis for changes in activation of endogenous Akt. GAPDH was used as a loading control. (F) Graph depicts the average fold change in the levels of phospho-Akt T308, phospho-Akt S473, and phospho-GSK3β S9 in three immunoblot experiments relative to VC-control. (G) Probing of VC-control, L3-control, and L3-Akt-AA lysates with phospho-Akt substrate antibody shows that dominant-negative Akt expression prevents the increased phosphorylation of many proteins caused by LCMT-1 knockdown (arrows show examples). The bracket with asterisk indicates phospho-rpS6, which ran at the bottom of this 7.5% SDS-PAGE gel. (H and I) Lysates were probed for changes in p70 and p85 S6K activation and rpS6 phosphorylation. Total p70 and p85 S6K and total rpS6 were used as controls. (J) Graph depicts the average fold change in the levels of phospho-p85, phospho-p70, and phospho-rpS6 in three immunoblot experiments relative to VC-control. Error bars represent SD of three independent experiments. For all graphs: * P ≤ .05. ** P ≤ .01.

    Article Snippet: Other antibodies used included sepharose bead-conjugated anti-HA-tag antibody used for immunoprecipitation (F-7 AC; Santa Cruz Biotechnologies, Santa Cruz, CA), anti-HA antibody used for Western blot analysis (16B12; Covance, Princeton, NJ), anti-SVST rabbit polyclonal antibody (gift from W. Hahn), anti-PyST rabbit polyclonal antibody [ ], PP2A Bα mouse monoclonal antibody (clone 2G9; Millipore, Bedford, MA), PP2Ac mouse monoclonal antibody (BD Transduction Laboratories, Franklin Lakes, NJ), unmethylated PP2A C subunit mouse monoclonal antibody (clone 1D6 [ ]; available from Millipore, Santa Cruz Biotechnology, or request to the corresponding author), p-Akt T308 and p-Akt S473 rabbit monoclonal antibodies (Epitomics, Burlingame, CA), GAPDH mouse monoclonal antibody (Abmart, Shanghai, China), Pme1 mouse monoclonal antibody (B12; Santa Cruz Biotechnology), mouse monoclonal antibodies to p-S6K (p70 T389 and p85 T412) and total rpS6, and rabbit polyclonal antibodies to p-GSK3β S9, p-rpS6 S235/236, total Akt, total p70/p85 S6K, and pAkt substrate ((R/K)X(R/K)XX(pT/pS)) obtained from Cell Signaling Technology (Danvers, MA).

    Techniques: Activation Assay, Transformation Assay, Stable Transfection, Expressing, Plasmid Preparation, Dominant Negative Mutation, Western Blot, SDS Page

    LCMT-1 knockdown activates Akt and S6K signaling in anchorage-independent conditions. (A) Equal numbers of VC and L3 cells were seeded on low-binding tissue culture dishes to analyze differences during anchorage-independent growth. After 1 week, suspension cells were weighed to assess growth. In the graph, data are presented as average fold change relative to VC for three independent experiments. (B) Lysates from VC and L3 suspension cells were analyzed by Western blot analysis for changes in activation of Akt. GAPDH and total Akt were used as loading controls. Western blot analysis for LCMT-1 confirmed the knockdown of LCMT-1 in suspension cultures. (C) Graph depicts the average levels of phospho-Akt T308, phospho-Akt S473, and phospho-GSK3β S9 in three independent immunoblot experiments as fold change relative to VC. (D) An anti-Akt phospho-substrate motif (R/K)X(R/K)XX(pT/pS) antibody was used to probe the VC and L3 suspension cell lysates. Arrows highlight some proteins with increased phosphorylation in the L3 cells relative to VC. Bracket with asterisk indicates phospho-rpS6, which is known to cross-react with this antibody. (E and F) Lysates were probed for changes in p70 and p85 S6K activation and rpS6 phosphorylation. Total p70 and p85 S6K and total rpS6 were used as loading controls. (G) Graph represents average fold change in the levels of phospho-p85, phospho-p70 and phospho-rpS6 in three independent immunoblot experiments relative to VC. Experiments were performed in triplicate and error bars in all panels represent SD. * P ≤ .05. ** P ≤ .01.

    Journal: Neoplasia (New York, N.Y.)

    Article Title: Circumventing Cellular Control of PP2A by Methylation Promotes Transformation in an Akt-Dependent Manner 1

    doi:

    Figure Lengend Snippet: LCMT-1 knockdown activates Akt and S6K signaling in anchorage-independent conditions. (A) Equal numbers of VC and L3 cells were seeded on low-binding tissue culture dishes to analyze differences during anchorage-independent growth. After 1 week, suspension cells were weighed to assess growth. In the graph, data are presented as average fold change relative to VC for three independent experiments. (B) Lysates from VC and L3 suspension cells were analyzed by Western blot analysis for changes in activation of Akt. GAPDH and total Akt were used as loading controls. Western blot analysis for LCMT-1 confirmed the knockdown of LCMT-1 in suspension cultures. (C) Graph depicts the average levels of phospho-Akt T308, phospho-Akt S473, and phospho-GSK3β S9 in three independent immunoblot experiments as fold change relative to VC. (D) An anti-Akt phospho-substrate motif (R/K)X(R/K)XX(pT/pS) antibody was used to probe the VC and L3 suspension cell lysates. Arrows highlight some proteins with increased phosphorylation in the L3 cells relative to VC. Bracket with asterisk indicates phospho-rpS6, which is known to cross-react with this antibody. (E and F) Lysates were probed for changes in p70 and p85 S6K activation and rpS6 phosphorylation. Total p70 and p85 S6K and total rpS6 were used as loading controls. (G) Graph represents average fold change in the levels of phospho-p85, phospho-p70 and phospho-rpS6 in three independent immunoblot experiments relative to VC. Experiments were performed in triplicate and error bars in all panels represent SD. * P ≤ .05. ** P ≤ .01.

    Article Snippet: Other antibodies used included sepharose bead-conjugated anti-HA-tag antibody used for immunoprecipitation (F-7 AC; Santa Cruz Biotechnologies, Santa Cruz, CA), anti-HA antibody used for Western blot analysis (16B12; Covance, Princeton, NJ), anti-SVST rabbit polyclonal antibody (gift from W. Hahn), anti-PyST rabbit polyclonal antibody [ ], PP2A Bα mouse monoclonal antibody (clone 2G9; Millipore, Bedford, MA), PP2Ac mouse monoclonal antibody (BD Transduction Laboratories, Franklin Lakes, NJ), unmethylated PP2A C subunit mouse monoclonal antibody (clone 1D6 [ ]; available from Millipore, Santa Cruz Biotechnology, or request to the corresponding author), p-Akt T308 and p-Akt S473 rabbit monoclonal antibodies (Epitomics, Burlingame, CA), GAPDH mouse monoclonal antibody (Abmart, Shanghai, China), Pme1 mouse monoclonal antibody (B12; Santa Cruz Biotechnology), mouse monoclonal antibodies to p-S6K (p70 T389 and p85 T412) and total rpS6, and rabbit polyclonal antibodies to p-GSK3β S9, p-rpS6 S235/236, total Akt, total p70/p85 S6K, and pAkt substrate ((R/K)X(R/K)XX(pT/pS)) obtained from Cell Signaling Technology (Danvers, MA).

    Techniques: Binding Assay, Western Blot, Activation Assay