Journal: Antimicrobial Agents and Chemotherapy

Article Title: Pan-antiviral effects of a PIKfyve inhibitor on respiratory virus infection in human nasal epithelium and mice

doi: 10.1128/aac.01050-23

Figure Lengend Snippet: H1N1 viral load in apical wash from ALI nasal epithelium treated with Apilimod or oseltamivir

Article Snippet: Materials Apilimod base, Apilimod mesylate, YM201636, oseltamivir phosphate, and oseltamivir carboxylate were purchased from MedChemExpress LLC (Monmouth Junction, NJ), ribavirin were purchased (confirmed by Pharmidex). .

Techniques:

Journal: Antimicrobial Agents and Chemotherapy

Article Title: Pan-antiviral effects of a PIKfyve inhibitor on respiratory virus infection in human nasal epithelium and mice

doi: 10.1128/aac.01050-23

Figure Lengend Snippet: Effects of Apilimod mesylate administered intranasally on H1N1-induced inflammation detected in bronchoalveolar lavage and nasal lavage of H1N1-infected mice a

Article Snippet: Materials Apilimod base, Apilimod mesylate, YM201636, oseltamivir phosphate, and oseltamivir carboxylate were purchased from MedChemExpress LLC (Monmouth Junction, NJ), ribavirin were purchased (confirmed by Pharmidex). .

Techniques: Inhibition, Saline, Virus

Journal: Virologica Sinica

Article Title: Dominant HA motif 131/132-NT shapes the phenotype of avian H9N2 influenza virus by modulating agglutination property, receptor specificity and infectivity in animals

doi: 10.1016/j.virs.2026.06.007

Figure Lengend Snippet: Effects of HA and NA on hemagglutination activity of H9N2 AIV at 4 °C and 37 °C. A Virus elution assay using CRBCs. Viruses were adsorbed onto 1% CRBCs at 4 °C for 30 min and then incubated at 37 °C to monitor virus elution by measuring hemagglutination titers over 420 min. B Hemagglutination titers of representative H9N2 AIVs with CRBCs at 4 °C and 37 °C. C Hemagglutination titers with CRBCs at 4 °C and 37 °C after pre-incubation with increasing concentrations of oseltamivir carboxylate.

Article Snippet: Viruses were twofold serially diluted in PBS with 0, 20, 100, 200, or 400 μM oseltamivir (MedChemExpress, USA), incubated at room temperature for 60 min, and assessed for hemagglutination with 1% CRBCs or TRBCs at 4 °C or 37 °C.

Techniques: Activity Assay, Virus, Incubation

Journal: American Journal of Physiology - Regulatory, Integrative and Comparative Physiology

Article Title: Greater natriuretic response to ENaC inhibition in male versus female Sprague-Dawley rats

doi: 10.1152/ajpregu.00060.2019

Figure Lengend Snippet: Antibodies used in immunoblotting

Article Snippet: Blots for ENaC subunits in addition to representative blots of actin and Coomassie are shown in . table ft1 table-wrap mode="anchored" t5 Table 1. caption a7 Antibody Target Primary Ab Supplier Ab Host Dilution Secondary Ab Supplier Ab Host Dilution α-ENaC StressMarq (SPC-403D) Rabbit 1:1,000 Invitrogen (SA5-10036) Goat 1:1,000 β-ENaC ProSci (Poway, CA) ( 21 , 34 ) Rabbit 1:2,000 Invitrogen (SA5-10036) Goat 1:1,000 γ-ENaC StressMarq (SPC-405) Rabbit 1:1,000 Invitrogen (SA5-10036) Goat 1:1,000 Actin Sigma (A1978) Mouse 1:20,000 Invitrogen ( A21057 ) Goat 1:10,000 Open in a separate window ENaC, epithelial sodium channel.

Techniques: