Journal: Neuroscience Bulletin

Article Title: A Two-Step GRIN Lens Coating for In Vivo Brain Imaging

doi: 10.1007/s12264-019-00356-x

Figure Lengend Snippet: Images of primary rat hippocampal neurons after 15 days in culture with coated and uncoated lenses. A Parylene-C-coated 1-mm diameter Go!Foton lenses. There was no parylene-C coating on the top end of the GRIN lens (arrow:coating interface). B Hippocampal neurons cultured with a coated lens maintained normal morphology. Left, coated lens; middle, neurons on the surface of lens (arrows); right, neurons near the lens. C Neurons cultured with an uncoated lens were characterized by the complete loss of neurites and changes in the cytoplasm. Left, uncoated lens; middle, neurons on the surface of lens (arrows); right, neurons near the lens. Scale bars, 100 μm.

Article Snippet: To determine whether the Go!Foton GRIN lens leached thallium ion into the solution, we soaked 8 uncoated lenses in 5 mL PBS for 20 days at room temperature.

Techniques: Cell Culture

Journal: Neuroscience Bulletin

Article Title: A Two-Step GRIN Lens Coating for In Vivo Brain Imaging

doi: 10.1007/s12264-019-00356-x

Figure Lengend Snippet: Two steps of coating enable successful in vivo imaging using Go!Foton lenses. A Neither active neurons nor blood vessels were observed under a Go!Foton lens without coating. B Active neurons (arrowhead) and blood clots (arrow) were observed under a GRIN lens coat with parylene-C alone. C Both active neurons (arrowhead) and blood vessels (arrow) were observed under GRIN lenses coated with both parylene-C and 50 μg/mL fibronectin. D Both active neurons (arrowhead) and blood vessels (arrow) were observed under a GrinTech lens. Scale bar, 100 μm.

Article Snippet: To determine whether the Go!Foton GRIN lens leached thallium ion into the solution, we soaked 8 uncoated lenses in 5 mL PBS for 20 days at room temperature.

Techniques: In Vivo Imaging

Journal: Neuroscience Bulletin

Article Title: A Two-Step GRIN Lens Coating for In Vivo Brain Imaging

doi: 10.1007/s12264-019-00356-x

Figure Lengend Snippet: Comparison of imaging performance. A–C Representative standard deviation projection images from miniScope recording via GRIN lenses coated with parylene-C and fibronectin at 100 μg/mL (Group 1) (A), 50 μg/mL (Group 2) (B), and 25 μg/mL (Group 3) (C). D Representative standard deviation projection image from miniScope recording via a GrinTech GRIN lens (Group 4). E Average ∆F/F of all the neurons from each group. Group 1: 2.46% ± 0.06%, 96 neurons from two mice; Group 2: 4.50% ± 0.13%, 224 neurons from two mice; Group 3: 5.55% ± 0.34%, 100 neurons from two mice; Group 4: 4.85% ± 0.14%, 366 neurons from three mice. ****P < 0.0001, one-way ANOVA and Tukey’s post hoc test. Histogram bars represent the mean value for all neurons, with error bars representing SEM. Scale bar, 100 μm.

Article Snippet: To determine whether the Go!Foton GRIN lens leached thallium ion into the solution, we soaked 8 uncoated lenses in 5 mL PBS for 20 days at room temperature.

Techniques: Comparison, Imaging, Standard Deviation