Journal: Diagnostic Pathology

Article Title: Podoplanin immunoexpression in odontogenic lesions: a systematic review, meta-analysis, and integrated bioinformatic analysis

doi: 10.1186/s13000-024-01540-y

Figure Lengend Snippet: Clinical characteristics and related data from included studies in this systematic review

Article Snippet: Zhang et al., 2013[ ] , China , CS , 30.1 13-55 , 9/7 , Yes , IHC , OKC , 16 , 4μm , Rabbit Mo PDPN (Proteintech group).

Techniques:

Journal: Diagnostic Pathology

Article Title: Podoplanin immunoexpression in odontogenic lesions: a systematic review, meta-analysis, and integrated bioinformatic analysis

doi: 10.1186/s13000-024-01540-y

Figure Lengend Snippet: Features of podoplanin immunoexpression in different odontogenic lesions

Article Snippet: Zhang et al., 2013[ ] , China , CS , 30.1 13-55 , 9/7 , Yes , IHC , OKC , 16 , 4μm , Rabbit Mo PDPN (Proteintech group).

Techniques: Expressing

Journal: Diagnostic Pathology

Article Title: Podoplanin immunoexpression in odontogenic lesions: a systematic review, meta-analysis, and integrated bioinformatic analysis

doi: 10.1186/s13000-024-01540-y

Figure Lengend Snippet: Forest plot comparing the PDPN immunoexpression of A OKC vs DC. B Funnel plot to check the publication bias. OKC=Odontogenic keratocyst, DC= Dentigerous cyst

Article Snippet: Zhang et al., 2013[ ] , China , CS , 30.1 13-55 , 9/7 , Yes , IHC , OKC , 16 , 4μm , Rabbit Mo PDPN (Proteintech group).

Techniques:

Journal: Diagnostic Pathology

Article Title: Podoplanin immunoexpression in odontogenic lesions: a systematic review, meta-analysis, and integrated bioinformatic analysis

doi: 10.1186/s13000-024-01540-y

Figure Lengend Snippet: Podoplanin immunoexpression in odontogenic lesions. PDPN is strongly expressed in DF, OKC, COC, AMs, AMU, AMPe, AMC, AOT, CEOT, AF and AFO. Its expression has been shown to be negative in GOC and OM. Furthermore, PDPN has been shown to participate in cell adhesion, migration and invasion through association with ERM proteins, which are expressed in different OL such as OKC, OOC, COC, AMs, AOT, CEOT, ODS and AF. IHC: Immunohistochemistry; OL: Odontogenic lesions; PDPN: Podoplanin; ERM: Ezrin, Radixin, Moesin; EZR: Ezrin; RDX: Radixin; MSN: Moesin; RhoA: Ras homolog gene family, member A; DF: Dental follicle; OKC: Odontogenic keratocyst; OOC: Orthokeratinized odontogenic cyst; COC: Calcifying odontogenic cyst; GOC: Glandular odontogenic cyst; AMs: Ameloblastoma solid; AMU: Ameloblastoma, Unicystic; AMPe: Ameloblastoma, Peripheral; AMC: Ameloblastic Carcinoma; AOT: Adenomatoid odontogenic tumor; CEOT: Calcifying epithelial odontogenic tumor; AF: Ameloblastic fibroma; AFO: Ameloblastic fibro-odontoma; ODS: Odontoma; OM: Odontogenic myxoma [ – , , ]

Article Snippet: Zhang et al., 2013[ ] , China , CS , 30.1 13-55 , 9/7 , Yes , IHC , OKC , 16 , 4μm , Rabbit Mo PDPN (Proteintech group).

Techniques: Expressing, Migration, Immunohistochemistry

Journal: bioRxiv

Article Title: Multiparametric senescent cell phenotyping reveals CD24 osteolineage cells as targets of senolytic therapy in the aged murine skeleton

doi: 10.1101/2023.01.12.523760

Figure Lengend Snippet: Key Resources Table

Article Snippet: pCMV6-Pdpn , Origene , MR201468.

Techniques: FACS, Recombinant, Red Blood Cell Lysis, Transfection, Lysis, Antibody Labeling, Staining, Software

Journal: Cell reports

Article Title: Crimean-Congo hemorrhagic fever survivors elicit protective non-neutralizing antibodies that target 11 overlapping regions on glycoprotein GP38

doi: 10.1016/j.celrep.2024.114502

Figure Lengend Snippet: (A) Matrix of competition-binning experiments. For on-yeast competition experiments (top left quadrant), results are displayed with surface-presented IgGs on the y axis and competitive pre-complexed Fabs on the x axis. For BLI competition assays (the other three quadrants), binning was performed in an IgG vs. IgG format. (B) Binning analysis of on-yeast competition assays of all 188 antibodies; each color represents 1 of 11 overlapping bins and the Unknown/Weak Affinity mAbs are shown in gray . Distribution of overlapping bins of the antibody panel (left) and by each donor (right). Total number of mAbs is indicated in the circular diagram and total mAbs from each donor are indicated above each bar graph.

Article Snippet: Models of CCHFV GP38 bound to Fabs of GP38-specific mAbs have been deposited at Worldwide Protein DataBank (wwPDB) under accession codes 8VVK, 8VVL, and 8VVW.

Techniques:

Journal: Cell reports

Article Title: Crimean-Congo hemorrhagic fever survivors elicit protective non-neutralizing antibodies that target 11 overlapping regions on glycoprotein GP38

doi: 10.1016/j.celrep.2024.114502

Figure Lengend Snippet: (A) Matrix of percent sequence identity of GP38 amino acid residues (AA) across six CCHFV isolates. (B) Single concentration BLI binding analysis of 188 antibodies to the six rGP38 proteins as a whole panel (top) and broken down by bin (bottom). Shades of green represent the number of rGP38 proteins bound by a single antibody (from 0 in gray to 6 in darkest green). Total number of mAbs is indicated in the circular diagram and total mAbs from each bin are indicated above the bar graph. (C) Carterra system HT-SPR binding analysis of six lead antibody candidates binding to six rGP38 proteins. The highest binding affinities are in dark green and the lowest binding affinities are in white. Calculated K D values appear in each rectangle of the heatmap; for samples that were off-rate limited, K D values are denoted as < the calculated K D . The one interaction for which a curve could not be fit is denoted as P.F.

Article Snippet: Models of CCHFV GP38 bound to Fabs of GP38-specific mAbs have been deposited at Worldwide Protein DataBank (wwPDB) under accession codes 8VVK, 8VVL, and 8VVW.

Techniques: Sequencing, Concentration Assay, Binding Assay

Journal: Cell reports

Article Title: Crimean-Congo hemorrhagic fever survivors elicit protective non-neutralizing antibodies that target 11 overlapping regions on glycoprotein GP38

doi: 10.1016/j.celrep.2024.114502

Figure Lengend Snippet: (A) Neutralization curves for CCHFV IbAr10200 tecVLPs, as measured by the reduction in luciferase activity compared with no-antibody treatment on Vero cells. (B–E) Neutralization curves of the indicated mAbs against authentic (B) CCHFV IbAr10200, (C) CCHFV Afg09, (D) CCHFV Turkey2004, and (E) CCHFV Oman as measured by the reduction in infection compared with no-antibody treatment on SW-13 cells. The average of n = 3 replicates each from two independent experiments ( n = 6 total) is shown for all neutralization curves.

Article Snippet: Models of CCHFV GP38 bound to Fabs of GP38-specific mAbs have been deposited at Worldwide Protein DataBank (wwPDB) under accession codes 8VVK, 8VVL, and 8VVW.

Techniques: Neutralization, Luciferase, Activity Assay, Infection

Journal: Cell reports

Article Title: Crimean-Congo hemorrhagic fever survivors elicit protective non-neutralizing antibodies that target 11 overlapping regions on glycoprotein GP38

doi: 10.1016/j.celrep.2024.114502

Figure Lengend Snippet: (A–C) IFNAR1 −/− mice were treated with the indicated mAbs at 1 mg/mouse 1 and 4 days post-challenge (2 mg total; n = 10 mice per group) with IbAr10200. (A) Survival curves (vehicle vs. test mAb), (B) associated mean weight loss, and (C) clinical score data are shown. (D–L) STAT1 −/− mice were challenged with (D–F) CCHFV-Afg09, (G–I) CCHFV-Turkey2004, or (J–L) CCHFV-Oman and then treated with 0.2 mg/mouse of mAb or vehicle 30 min post-exposure (n = 5–6 mice per study; represented by 2 replicate studies). (D, G, and J) Survival curves. (E, H, and K) Associated mean weight loss. (F, I, and L) Clinical scores are defined as: 1 = decreased grooming and/or ruffled fur; 2 = subdued behavior when un-stimulated; 3 = lethargy, hunched posture, and/or subdued behavior even when stimulated; 4 = bleeding, unresponsiveness, severe weakness, or inability to walk. Mice scoring a 4 were considered moribund and were humanely euthanized based on IACUC-approved criteria (denoted as X over white).

Article Snippet: Models of CCHFV GP38 bound to Fabs of GP38-specific mAbs have been deposited at Worldwide Protein DataBank (wwPDB) under accession codes 8VVK, 8VVL, and 8VVW.

Techniques:

Journal: Frontiers in Immunology

Article Title: A Novel Bacterial Artificial Chromosome-Transgenic Podoplanin–Cre Mouse Targets Lymphoid Organ Stromal Cells in vivo

doi: 10.3389/fimmu.2011.00050

Figure Lengend Snippet: Generation of the Bacterial Artificial Chromosome (BAC) transgenic Pdpn–Cre mouse . (A) Schematic representation of BAC mutagenesis with introduction of the Cre-recombinase gene into the pdpn locus on BAC RP23 146H1. Colored arrows indicate primer location of Cre–PCR (green), 5′ATG PCR (blue) and 3′polyA PCR (red). (B) Initial screening of successfully recombined BAC by PCR for Cre-recombinase, 5′ATG region, and 3′polyA region. (C) PCR-positive BAC clones were examined by southern blot using a digoxigenin-labeled 5′ATG probe covering transgene and BAC sequences on the recombined (rec), but not the wild-type BAC (wt). (D) PCR analysis of purified genomic DNA from a cohort of potential founder mice. BAC indicates positive control DNA.

Article Snippet: Sections were incubated over night at 4°C with the following monoclonal antibodies: anti-gp38/podoplanin (clone8.1.1, BD Biosciences), Alexa647-conjugated anti-B220 (BD Biosciences), anti-EYFP (Clontech), Alexa647-conjugated anti-CD31 (eBioscience), anti-LyveI (eBioscience), or anti-βGal (Abcam).

Techniques: Transgenic Assay, Mutagenesis, Clone Assay, Southern Blot, Labeling, Purification, Positive Control

Journal: Frontiers in Immunology

Article Title: A Novel Bacterial Artificial Chromosome-Transgenic Podoplanin–Cre Mouse Targets Lymphoid Organ Stromal Cells in vivo

doi: 10.3389/fimmu.2011.00050

Figure Lengend Snippet: Validation of transgene expression and Cre-mediated recombination . Filial generation 1 of Pdpn–Cre founder 14 was crossed to R26-EYFP reporter mice. (A) Flow cytometric analysis of CD45-depleted cell suspensions utilized the distinct forward and side scatter properties (left dot plot) and lack of CD45-staining in EYFP + population as found in right dot plot, upper left quadrant. (B) VCAM-1 (left histogram) and ICAM-1 (right histogram) expression on CD45 − EYFP + stromal cells from LNs of pdpn–Cre,R26-EYFP mice. (C–F) Stromal cell subsets in CD45-depleted cell preparations from LNs (C) and spleen (E) according to CD31 and pdpn expression. EYFP expression was determined on the indicated LN (D) or splenic (F) stromal cell subsets from Pdpn–Cre,R26-EYFP (black lines) and C57BL/6 control mice (gray shaded). Values in histograms in (D,F) indicate mean percentage ± SEM of EYFP-positive cells ( n = 3 mice).

Article Snippet: Sections were incubated over night at 4°C with the following monoclonal antibodies: anti-gp38/podoplanin (clone8.1.1, BD Biosciences), Alexa647-conjugated anti-B220 (BD Biosciences), anti-EYFP (Clontech), Alexa647-conjugated anti-CD31 (eBioscience), anti-LyveI (eBioscience), or anti-βGal (Abcam).

Techniques: Expressing, Staining

Journal: Frontiers in Immunology

Article Title: A Novel Bacterial Artificial Chromosome-Transgenic Podoplanin–Cre Mouse Targets Lymphoid Organ Stromal Cells in vivo

doi: 10.3389/fimmu.2011.00050

Figure Lengend Snippet: Pdpn–Cre transgene-expressing cells are non-hematopoietic stromal cells . (A) Flow cytometric analysis of Pdpn–Cre,R26-EYFP LN single-cell suspensions. Appropriate gates were established (boxed area) for subsequent analysis of EYFP + and EYFP − populations. Histogram shows analysis of CD45 expression on EYFP + (black line) and EYFP − (gray shaded) cells. (B) Confocal microscopy analysis of a Pdpn–Cre,R26-EYFP LN section stained for T lymphocytes (CD4 in red), B lymphocytes (B220 in blue), and transgene-expressing cells (EYFP in green), scale bar equals 50 μm, boxed area defines area for magnified view in (C) and three-dimensional rendering in (D) , scale bars equal 20 μm, Fo: B cell follicle.

Article Snippet: Sections were incubated over night at 4°C with the following monoclonal antibodies: anti-gp38/podoplanin (clone8.1.1, BD Biosciences), Alexa647-conjugated anti-B220 (BD Biosciences), anti-EYFP (Clontech), Alexa647-conjugated anti-CD31 (eBioscience), anti-LyveI (eBioscience), or anti-βGal (Abcam).

Techniques: Expressing, Confocal Microscopy, Staining

Journal: Frontiers in Immunology

Article Title: A Novel Bacterial Artificial Chromosome-Transgenic Podoplanin–Cre Mouse Targets Lymphoid Organ Stromal Cells in vivo

doi: 10.3389/fimmu.2011.00050

Figure Lengend Snippet: Localization and morphological phenotype of transgene-expressing stromal cells in LNs of Pdpn–Cre,R26-EYFP mice . (A) Histological section of inguinal LN from a Pdpn–Cre,R26-EYFP mouse stained for B cells (B220 in blue), Pdpn expressing stromal cells (red), and Cre-mediated transgene expression (EYFP, green). Inserts in the left panel mark the regions taken to acquire more detailed images in (B,C) . Scale bar equals 200 μm. (B) Transgene-expressing stromal cells in the T cell zone with typical FRC structure marked with white arrow in the right panel. (C) Transgene-positive stromal cell in the subcapsular sinus (SCS) region visualized with anti-LyveI and anti-EYFP staining. Sinus-lining LEC is marked with white arrow in the right panel. Scale bars in (B,C) equal 10 μm.

Article Snippet: Sections were incubated over night at 4°C with the following monoclonal antibodies: anti-gp38/podoplanin (clone8.1.1, BD Biosciences), Alexa647-conjugated anti-B220 (BD Biosciences), anti-EYFP (Clontech), Alexa647-conjugated anti-CD31 (eBioscience), anti-LyveI (eBioscience), or anti-βGal (Abcam).

Techniques: Expressing, Staining

Journal: Frontiers in Immunology

Article Title: A Novel Bacterial Artificial Chromosome-Transgenic Podoplanin–Cre Mouse Targets Lymphoid Organ Stromal Cells in vivo

doi: 10.3389/fimmu.2011.00050

Figure Lengend Snippet: Distinct localization and morphology of Pdpn–Cre + cells in spleen . (A) Splenic white pulp area of a Pdpn–Cre,R26-EYFP mouse stained for B cells (B220 in blue), Pdpn expressing stromal cells (red), and Cre-mediated transgene expression (EYFP, green). Insert in the right panel marks the region used for more detailed image in (B) . Scale bar equals 100 μm. (B) Boxed area in (A) showing the central localization of transgene-expressing Pdpn + cells. (C) Longitudinal section of the CD31 + central arteriole (c.a.) with surrounding transgene-expressing perivascular stromal cells (marked as PSC in right panel). Scale bar equals 20 μm. (D) 3D projection of transverse section of the CD31 + central arteriole (c.a.) and surrounding pdpn + EYFP + perivascular stromal cells. Note the tube-like structures extending into the surrounding T cell zone. Scale bar equals 50 μm.

Article Snippet: Sections were incubated over night at 4°C with the following monoclonal antibodies: anti-gp38/podoplanin (clone8.1.1, BD Biosciences), Alexa647-conjugated anti-B220 (BD Biosciences), anti-EYFP (Clontech), Alexa647-conjugated anti-CD31 (eBioscience), anti-LyveI (eBioscience), or anti-βGal (Abcam).

Techniques: Staining, Expressing

Journal: Frontiers in Immunology

Article Title: A Novel Bacterial Artificial Chromosome-Transgenic Podoplanin–Cre Mouse Targets Lymphoid Organ Stromal Cells in vivo

doi: 10.3389/fimmu.2011.00050

Figure Lengend Snippet: βgal expression in FRCs and LECs in Pdpn–Cre,R26-LacZ mice leads to activation of βgal-specific TCR transgenic T cells . (A) Pdpn–Cre mice were crossed to R26-LacZ reporter mice and βgal expression in LNs was detected by immunofluorescence analysis. Section of inguinal LN from Pdpn-Cre,R26-LacZ mouse stained for βgal (green), Pdpn (blue), and CD4 (red). Scale bar equals 100 μm. (B) Stimulation of βgal-specific TCR transgenic T cells by stromal βgal expression was determined by flow cytometry using CFSE-labeled congenic Bg1 and Bg2 cells. CFSE dilution in blood, spleen, and LNs was measured on day 5 after adoptive transfer in Pdpn–Cre,R26-LacZ mice (black lines) or Cre-negative littermates (gray shaded). Bg1 or Bg2 cells were adoptively transferred either alone or in combination. Values in the histograms indicate percentage of proliferated TCR transgenic cells; proliferation in Cre-negative littermates was always below 5%.

Article Snippet: Sections were incubated over night at 4°C with the following monoclonal antibodies: anti-gp38/podoplanin (clone8.1.1, BD Biosciences), Alexa647-conjugated anti-B220 (BD Biosciences), anti-EYFP (Clontech), Alexa647-conjugated anti-CD31 (eBioscience), anti-LyveI (eBioscience), or anti-βGal (Abcam).

Techniques: Expressing, Activation Assay, Transgenic Assay, Immunofluorescence, Staining, Flow Cytometry, Labeling, Adoptive Transfer Assay