goscripttm Search Results


90
Promega goscripttm reverse transcription system
Goscripttm Reverse Transcription System, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Promega goscripttm reverse transcriptase
Goscripttm Reverse Transcriptase, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Promega goscripttm rt reagent kit
Goscripttm Rt Reagent Kit, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Promega goscript rt mix for one-step qrt-pcr
Goscript Rt Mix For One Step Qrt Pcr, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Promega goscripttm
Goscripttm, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Promega goscripttm reverse transcriptase 160 u/μl
Goscripttm Reverse Transcriptase 160 U/μl, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Promega goscripttm reverse transcriptase system first-strand synthesis system
Goscripttm Reverse Transcriptase System First Strand Synthesis System, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Promega trifasttm and goscripttm reverse transcription system
Trifasttm And Goscripttm Reverse Transcription System, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Promega goscripttm reverse transcriptase with random primers
Goscripttm Reverse Transcriptase With Random Primers, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Promega goscripttm reverse transcriptase oligo(dt) primers
Goscripttm Reverse Transcriptase Oligo(Dt) Primers, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Promega goscripttm reverse transcription system protocol
Validation of hsa_circ_0003416. (A) Melting curve. (B) Gel electrophoresis of the polymerase chain reaction (PCR) product. (C) Divergent primers (◄►) for detecting hsa_circ_0003416 in complementary <t>DNA</t> <t>(cDNA)</t> but not in genomic DNA (gDNA). (d) Sanger sequencing of the back‐spliced junction (↓) of hsa_circ_0003416
Goscripttm Reverse Transcription System Protocol, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Promega goscripttm qpcr master mix
MG-Exos protect endothelia cells against the oxidative effects by modulating the keap1/Nrf2/HO-1 signaling pathway. (a) Representative immunofluorescence images of the Nrf2's expression of bEnd.3 after Vehicle or MG-Exo treatment when exposed to H 2 O 2 -induced oxidative stress. Scale bar = 100 μ m. (b) Quantification of mean fluorescence intensity (MFI) in (a). (c) Western blotting analysis of the protein levels of keap1, Nrf2, and HO-1 in H 2 O 2 plus Vehicle or MG-Exo treatment groups. (d–g) <t>qPCR</t> verification of the mRNA levels of downstream antioxidative-related genes including NQo1 , Gclc , Cat , and Gsx1 per group when exposed to H 2 O 2 -induced oxidative stress. The data are presented as the means ± SD; n = 6 per group. ∗ p < 0.05 and ∗∗ p < 0.01 compared with different treatment groups.
Goscripttm Qpcr Master Mix, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Validation of hsa_circ_0003416. (A) Melting curve. (B) Gel electrophoresis of the polymerase chain reaction (PCR) product. (C) Divergent primers (◄►) for detecting hsa_circ_0003416 in complementary DNA (cDNA) but not in genomic DNA (gDNA). (d) Sanger sequencing of the back‐spliced junction (↓) of hsa_circ_0003416

Journal: Journal of Clinical Laboratory Analysis

Article Title: Expression and clinical significance of circular RNA hsa_circ_0003416 in pediatric pulmonary arterial hypertension associated with congenital heart disease

doi: 10.1002/jcla.24273

Figure Lengend Snippet: Validation of hsa_circ_0003416. (A) Melting curve. (B) Gel electrophoresis of the polymerase chain reaction (PCR) product. (C) Divergent primers (◄►) for detecting hsa_circ_0003416 in complementary DNA (cDNA) but not in genomic DNA (gDNA). (d) Sanger sequencing of the back‐spliced junction (↓) of hsa_circ_0003416

Article Snippet: RNA was reverse transcribed into complementary DNA (cDNA) using the GoScriptTM Reverse Transcription System Protocol (Promega).

Techniques: Biomarker Discovery, Nucleic Acid Electrophoresis, Polymerase Chain Reaction, Sequencing

MG-Exos protect endothelia cells against the oxidative effects by modulating the keap1/Nrf2/HO-1 signaling pathway. (a) Representative immunofluorescence images of the Nrf2's expression of bEnd.3 after Vehicle or MG-Exo treatment when exposed to H 2 O 2 -induced oxidative stress. Scale bar = 100 μ m. (b) Quantification of mean fluorescence intensity (MFI) in (a). (c) Western blotting analysis of the protein levels of keap1, Nrf2, and HO-1 in H 2 O 2 plus Vehicle or MG-Exo treatment groups. (d–g) qPCR verification of the mRNA levels of downstream antioxidative-related genes including NQo1 , Gclc , Cat , and Gsx1 per group when exposed to H 2 O 2 -induced oxidative stress. The data are presented as the means ± SD; n = 6 per group. ∗ p < 0.05 and ∗∗ p < 0.01 compared with different treatment groups.

Journal: Oxidative Medicine and Cellular Longevity

Article Title: Microglia-Derived Exosomes Improve Spinal Cord Functional Recovery after Injury via Inhibiting Oxidative Stress and Promoting the Survival and Function of Endothelia Cells

doi: 10.1155/2021/1695087

Figure Lengend Snippet: MG-Exos protect endothelia cells against the oxidative effects by modulating the keap1/Nrf2/HO-1 signaling pathway. (a) Representative immunofluorescence images of the Nrf2's expression of bEnd.3 after Vehicle or MG-Exo treatment when exposed to H 2 O 2 -induced oxidative stress. Scale bar = 100 μ m. (b) Quantification of mean fluorescence intensity (MFI) in (a). (c) Western blotting analysis of the protein levels of keap1, Nrf2, and HO-1 in H 2 O 2 plus Vehicle or MG-Exo treatment groups. (d–g) qPCR verification of the mRNA levels of downstream antioxidative-related genes including NQo1 , Gclc , Cat , and Gsx1 per group when exposed to H 2 O 2 -induced oxidative stress. The data are presented as the means ± SD; n = 6 per group. ∗ p < 0.05 and ∗∗ p < 0.01 compared with different treatment groups.

Article Snippet: Single-strand cDNA was reverse transcribed from 1 μ g total RNA from each sample using GoScriptTM Reverse Transcription System (Promega Corporation, USA) according to the manufacturer's instructions. qRT-PCR was performed by GoScriptTM qPCR Master Mix (Promega) using a BRYT Green master mix.

Techniques: Immunofluorescence, Expressing, Fluorescence, Western Blot