goscripttm Search Results


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MG-Exos protect endothelia cells against the oxidative effects by modulating the keap1/Nrf2/HO-1 signaling pathway. (a) Representative immunofluorescence images of the Nrf2's expression of bEnd.3 after Vehicle or MG-Exo treatment when exposed to H 2 O 2 -induced oxidative stress. Scale bar = 100 μ m. (b) Quantification of mean fluorescence intensity (MFI) in (a). (c) Western blotting analysis of the protein levels of keap1, Nrf2, and HO-1 in H 2 O 2 plus Vehicle or MG-Exo treatment groups. (d–g) <t>qPCR</t> verification of the mRNA levels of downstream antioxidative-related genes including NQo1 , Gclc , Cat , and Gsx1 per group when exposed to H 2 O 2 -induced oxidative stress. The data are presented as the means ± SD; n = 6 per group. ∗ p < 0.05 and ∗∗ p < 0.01 compared with different treatment groups.
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MG-Exos protect endothelia cells against the oxidative effects by modulating the keap1/Nrf2/HO-1 signaling pathway. (a) Representative immunofluorescence images of the Nrf2's expression of bEnd.3 after Vehicle or MG-Exo treatment when exposed to H 2 O 2 -induced oxidative stress. Scale bar = 100 μ m. (b) Quantification of mean fluorescence intensity (MFI) in (a). (c) Western blotting analysis of the protein levels of keap1, Nrf2, and HO-1 in H 2 O 2 plus Vehicle or MG-Exo treatment groups. (d–g) <t>qPCR</t> verification of the mRNA levels of downstream antioxidative-related genes including NQo1 , Gclc , Cat , and Gsx1 per group when exposed to H 2 O 2 -induced oxidative stress. The data are presented as the means ± SD; n = 6 per group. ∗ p < 0.05 and ∗∗ p < 0.01 compared with different treatment groups.
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Image Search Results


MG-Exos protect endothelia cells against the oxidative effects by modulating the keap1/Nrf2/HO-1 signaling pathway. (a) Representative immunofluorescence images of the Nrf2's expression of bEnd.3 after Vehicle or MG-Exo treatment when exposed to H 2 O 2 -induced oxidative stress. Scale bar = 100 μ m. (b) Quantification of mean fluorescence intensity (MFI) in (a). (c) Western blotting analysis of the protein levels of keap1, Nrf2, and HO-1 in H 2 O 2 plus Vehicle or MG-Exo treatment groups. (d–g) qPCR verification of the mRNA levels of downstream antioxidative-related genes including NQo1 , Gclc , Cat , and Gsx1 per group when exposed to H 2 O 2 -induced oxidative stress. The data are presented as the means ± SD; n = 6 per group. ∗ p < 0.05 and ∗∗ p < 0.01 compared with different treatment groups.

Journal: Oxidative Medicine and Cellular Longevity

Article Title: Microglia-Derived Exosomes Improve Spinal Cord Functional Recovery after Injury via Inhibiting Oxidative Stress and Promoting the Survival and Function of Endothelia Cells

doi: 10.1155/2021/1695087

Figure Lengend Snippet: MG-Exos protect endothelia cells against the oxidative effects by modulating the keap1/Nrf2/HO-1 signaling pathway. (a) Representative immunofluorescence images of the Nrf2's expression of bEnd.3 after Vehicle or MG-Exo treatment when exposed to H 2 O 2 -induced oxidative stress. Scale bar = 100 μ m. (b) Quantification of mean fluorescence intensity (MFI) in (a). (c) Western blotting analysis of the protein levels of keap1, Nrf2, and HO-1 in H 2 O 2 plus Vehicle or MG-Exo treatment groups. (d–g) qPCR verification of the mRNA levels of downstream antioxidative-related genes including NQo1 , Gclc , Cat , and Gsx1 per group when exposed to H 2 O 2 -induced oxidative stress. The data are presented as the means ± SD; n = 6 per group. ∗ p < 0.05 and ∗∗ p < 0.01 compared with different treatment groups.

Article Snippet: Single-strand cDNA was reverse transcribed from 1 μ g total RNA from each sample using GoScriptTM Reverse Transcription System (Promega Corporation, USA) according to the manufacturer's instructions. qRT-PCR was performed by GoScriptTM qPCR Master Mix (Promega) using a BRYT Green master mix.

Techniques: Immunofluorescence, Expressing, Fluorescence, Western Blot