goat anti-rabbit igg h+l secondary antibody Thermo Fisher Search Results


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  • 98
    Thermo Fisher secondary antibody
    Secondary Antibody, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 98/100, based on 10102 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Thermo Fisher goat anti rabbit igg h l cross adsorbed secondary antibody
    Loss of VCP induces EMT A. 72 hours after transfection of A549, H358 or immortalized human bronchiolar HPLD-1 cells with siRNA against VCP or non-targeting cell lysates were prepared and western blot analysis of EMT markers Vimentin and E-cadherin was performed. B. Fluorescence staining for E-cadherin and Vimentin in A549 cells. After 24 hrs of transfection either with non-targeting siRNA (siNT) or with siRNAs targeting VCP (siVCP1 or siVCP2) cells were trypsinized and plated on chamber slides and stained for EMT markers. i, iii and v: E-cadherin was detected using <t>Alexa</t> <t>Fluor</t> 488 goat anti-rabbit <t>IgG</t> (green). ii, iv and vi: overlay of respective E-cadherin and F-actin (Alexa Fluor 568 Phalloidin; red) staining with DAPI counter stain. a, c and e: Vimentin was detected using Alexa Fluor 488 goat anti-rabbit IgG (green). b, d and f: overlay of respective Vimentin and F-actin (Alexa Fluor 568 Phalloidin; red) staining with DAPI counter stain. C. A549 cells were prepared as described in B and F-actin was detected with Alexa Fluor 568 Phalloidin (red). Re-organization of actin cytoskeleton through destruction and cellular protrusion formation is indicated by arrows.
    Goat Anti Rabbit Igg H L Cross Adsorbed Secondary Antibody, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 21670 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Thermo Fisher goat anti rabbit igg h l secondary antibody
    USF-1 interacts with the ILEI promoter sequence. A, 5′-biotin–tagged ILEI promoter oligonucleotide constructs with WT or mutant E-box. Biotin pulldown analysis of nuclear extracts from 501-Mel melanoma cells, followed by immunoblot for USF-1. B, PCR primers flanking the ILEI promoter E-box used in ChIP analysis. ChIP analysis of 501-Mel melanoma cell lines immunoprecipitated with control <t>IgG</t> or USF-1 antibody. PCR analysis conducted with primers targeting FAM3C, TYR, or HO1 promoter.
    Goat Anti Rabbit Igg H L Secondary Antibody, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1876 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher goat anti mouse igg h l cross adsorbed secondary antibody
    USF-1 interacts with the ILEI promoter sequence. A, 5′-biotin–tagged ILEI promoter oligonucleotide constructs with WT or mutant E-box. Biotin pulldown analysis of nuclear extracts from 501-Mel melanoma cells, followed by immunoblot for USF-1. B, PCR primers flanking the ILEI promoter E-box used in ChIP analysis. ChIP analysis of 501-Mel melanoma cell lines immunoprecipitated with control <t>IgG</t> or USF-1 antibody. PCR analysis conducted with primers targeting FAM3C, TYR, or HO1 promoter.
    Goat Anti Mouse Igg H L Cross Adsorbed Secondary Antibody, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 14005 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher goat anti rabbit igg h l superclonal secondary antibody
    USF-1 interacts with the ILEI promoter sequence. A, 5′-biotin–tagged ILEI promoter oligonucleotide constructs with WT or mutant E-box. Biotin pulldown analysis of nuclear extracts from 501-Mel melanoma cells, followed by immunoblot for USF-1. B, PCR primers flanking the ILEI promoter E-box used in ChIP analysis. ChIP analysis of 501-Mel melanoma cell lines immunoprecipitated with control <t>IgG</t> or USF-1 antibody. PCR analysis conducted with primers targeting FAM3C, TYR, or HO1 promoter.
    Goat Anti Rabbit Igg H L Superclonal Secondary Antibody, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 566 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher donkey anti rabbit igg h l highly cross adsorbed secondary antibody
    USF-1 interacts with the ILEI promoter sequence. A, 5′-biotin–tagged ILEI promoter oligonucleotide constructs with WT or mutant E-box. Biotin pulldown analysis of nuclear extracts from 501-Mel melanoma cells, followed by immunoblot for USF-1. B, PCR primers flanking the ILEI promoter E-box used in ChIP analysis. ChIP analysis of 501-Mel melanoma cell lines immunoprecipitated with control <t>IgG</t> or USF-1 antibody. PCR analysis conducted with primers targeting FAM3C, TYR, or HO1 promoter.
    Donkey Anti Rabbit Igg H L Highly Cross Adsorbed Secondary Antibody, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 9653 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher f ab 2 rabbit anti goat igg h l secondary antibody
    USF-1 interacts with the ILEI promoter sequence. A, 5′-biotin–tagged ILEI promoter oligonucleotide constructs with WT or mutant E-box. Biotin pulldown analysis of nuclear extracts from 501-Mel melanoma cells, followed by immunoblot for USF-1. B, PCR primers flanking the ILEI promoter E-box used in ChIP analysis. ChIP analysis of 501-Mel melanoma cell lines immunoprecipitated with control <t>IgG</t> or USF-1 antibody. PCR analysis conducted with primers targeting FAM3C, TYR, or HO1 promoter.
    F Ab 2 Rabbit Anti Goat Igg H L Secondary Antibody, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Loss of VCP induces EMT A. 72 hours after transfection of A549, H358 or immortalized human bronchiolar HPLD-1 cells with siRNA against VCP or non-targeting cell lysates were prepared and western blot analysis of EMT markers Vimentin and E-cadherin was performed. B. Fluorescence staining for E-cadherin and Vimentin in A549 cells. After 24 hrs of transfection either with non-targeting siRNA (siNT) or with siRNAs targeting VCP (siVCP1 or siVCP2) cells were trypsinized and plated on chamber slides and stained for EMT markers. i, iii and v: E-cadherin was detected using Alexa Fluor 488 goat anti-rabbit IgG (green). ii, iv and vi: overlay of respective E-cadherin and F-actin (Alexa Fluor 568 Phalloidin; red) staining with DAPI counter stain. a, c and e: Vimentin was detected using Alexa Fluor 488 goat anti-rabbit IgG (green). b, d and f: overlay of respective Vimentin and F-actin (Alexa Fluor 568 Phalloidin; red) staining with DAPI counter stain. C. A549 cells were prepared as described in B and F-actin was detected with Alexa Fluor 568 Phalloidin (red). Re-organization of actin cytoskeleton through destruction and cellular protrusion formation is indicated by arrows.

    Journal: Oncotarget

    Article Title: Regulation of VCP/p97 demonstrates the critical balance between cell death and epithelial-mesenchymal transition (EMT) downstream of ER stress

    doi:

    Figure Lengend Snippet: Loss of VCP induces EMT A. 72 hours after transfection of A549, H358 or immortalized human bronchiolar HPLD-1 cells with siRNA against VCP or non-targeting cell lysates were prepared and western blot analysis of EMT markers Vimentin and E-cadherin was performed. B. Fluorescence staining for E-cadherin and Vimentin in A549 cells. After 24 hrs of transfection either with non-targeting siRNA (siNT) or with siRNAs targeting VCP (siVCP1 or siVCP2) cells were trypsinized and plated on chamber slides and stained for EMT markers. i, iii and v: E-cadherin was detected using Alexa Fluor 488 goat anti-rabbit IgG (green). ii, iv and vi: overlay of respective E-cadherin and F-actin (Alexa Fluor 568 Phalloidin; red) staining with DAPI counter stain. a, c and e: Vimentin was detected using Alexa Fluor 488 goat anti-rabbit IgG (green). b, d and f: overlay of respective Vimentin and F-actin (Alexa Fluor 568 Phalloidin; red) staining with DAPI counter stain. C. A549 cells were prepared as described in B and F-actin was detected with Alexa Fluor 568 Phalloidin (red). Re-organization of actin cytoskeleton through destruction and cellular protrusion formation is indicated by arrows.

    Article Snippet: Antibodies used for study VCP #2649, Bip #3183, CHOP #2895, eIF2α #9722 and p-eIF2α #9721, IRE1α #3294, PERK #5683, JNK #9258, p-JNK 4668, p38 #8690, P-p38 #4511, LC3 #3868, Cleaved Caspase3 #9664, Calnexin #2679, PDI #3501, E-cadherin #3195, Vimentin #5741, Zeb1 #3396, Claudin1 #4933, Snail #3879, Slug #9585, Integrin β3 #4702, Akt #9272, P-Akt #9271, mTOR #2972, P-mTOR #2971 (Cell Signaling Technologies Inc. Danvers, MA 01923); GAPDH #FL335 (Santa Cruz); Alexa Fluor 488 goat anti-rabbit IgG #A11034 and Alexa Fluor 546 goat anti-rabbit IgG #A11010 (Molecular Probes, Invitrogen detection technologies, Eugene, OR.

    Techniques: Transfection, Western Blot, Fluorescence, Staining

    Loss of VCP induces ER stress A. Western blot analysis of different proteins involved in unfolded protein response and ER stress response. Cells were transfected with either non targeting (siNT) siRNA or two different siRNAs targeting VCP (siVCP1 and siVCP2). After 72 hrs of transfection, cells were harvested and analyzed for protein expression. B. Fluorescence staining for Chop and Calnexin in A549 and H358 cells. After 24 hrs of transfection either with non-targeting siRNA (siNT) or with siRNAs targeting VCP (siVCP1 or siVCP2) cells were trypsinized and plated on chamber slides. After 48 hours cells were fixed and stained for Chop and Calnexin. i, iii and v: Chop was detected using Alexa Fluor 488 rabbit anti-mouse IgG (green). ii, iv and vi: overlay of respective Chop and F-actin (Alexa Fluor 568 Phalloidin; red) staining with DAPI counter stain. a, c and e: Calnexin was detected using Alexa Fluor 488 goat anti-rabbit IgG (green). b, d and f: overlay of respective Calnexin and F-actin (Alexa Fluor 568 Phalloidin; red) staining with DAPI counter stain.

    Journal: Oncotarget

    Article Title: Regulation of VCP/p97 demonstrates the critical balance between cell death and epithelial-mesenchymal transition (EMT) downstream of ER stress

    doi:

    Figure Lengend Snippet: Loss of VCP induces ER stress A. Western blot analysis of different proteins involved in unfolded protein response and ER stress response. Cells were transfected with either non targeting (siNT) siRNA or two different siRNAs targeting VCP (siVCP1 and siVCP2). After 72 hrs of transfection, cells were harvested and analyzed for protein expression. B. Fluorescence staining for Chop and Calnexin in A549 and H358 cells. After 24 hrs of transfection either with non-targeting siRNA (siNT) or with siRNAs targeting VCP (siVCP1 or siVCP2) cells were trypsinized and plated on chamber slides. After 48 hours cells were fixed and stained for Chop and Calnexin. i, iii and v: Chop was detected using Alexa Fluor 488 rabbit anti-mouse IgG (green). ii, iv and vi: overlay of respective Chop and F-actin (Alexa Fluor 568 Phalloidin; red) staining with DAPI counter stain. a, c and e: Calnexin was detected using Alexa Fluor 488 goat anti-rabbit IgG (green). b, d and f: overlay of respective Calnexin and F-actin (Alexa Fluor 568 Phalloidin; red) staining with DAPI counter stain.

    Article Snippet: Antibodies used for study VCP #2649, Bip #3183, CHOP #2895, eIF2α #9722 and p-eIF2α #9721, IRE1α #3294, PERK #5683, JNK #9258, p-JNK 4668, p38 #8690, P-p38 #4511, LC3 #3868, Cleaved Caspase3 #9664, Calnexin #2679, PDI #3501, E-cadherin #3195, Vimentin #5741, Zeb1 #3396, Claudin1 #4933, Snail #3879, Slug #9585, Integrin β3 #4702, Akt #9272, P-Akt #9271, mTOR #2972, P-mTOR #2971 (Cell Signaling Technologies Inc. Danvers, MA 01923); GAPDH #FL335 (Santa Cruz); Alexa Fluor 488 goat anti-rabbit IgG #A11034 and Alexa Fluor 546 goat anti-rabbit IgG #A11010 (Molecular Probes, Invitrogen detection technologies, Eugene, OR.

    Techniques: Western Blot, Transfection, Expressing, Fluorescence, Staining

    Immunolocalization of SVMPs with vascular basement membrane on cremaster muscle ex vivo . Isolated cremaster muscles were incubated for 15 min with equi-hemorrhagic amounts of either BaP1 (PI, 30 μg), BlatH1 (PII, 3.5 μg) or CsH1 (PIII, 15 μg) SVMPs labeled with Alexa Fluor 647 (blue). Control tissues were incubated with the SVMPs without labeling and no fluorescence was detected. Whole tissues were fixed with 4% paraformaldehyde and immunostained with anti-collagen IV following the secondary antibody labeled with Alexa Fluor 488 (green). Tissues were visualized in a Zeiss LSM 5 Pascal laser-scanning confocal microscope. Three-dimensional reconstitution of the images and analysis of co-localization were carried out with the IMARIS x64 7.4.2 software as described in Methods. (A) Distribution of the SVMPs in the cremaster muscle tissue. Scale bar represents 150 μm. (B) White areas represent co-localization of the SVMPs (blue) with collagen IV (green) of vascular basement membrane in PCV, arterioles, and capillaries. Scale bar represents 20 μm. Results are expressed as the mean ± SEM of (C) percentage of material of SMVPs co-localized with collagen IV of vascular basement membrane, and (D) Pearson´s correlation coefficient of at least four vessels type per tissue (n = 3). *p

    Journal: PLoS Neglected Tropical Diseases

    Article Title: Tissue Localization and Extracellular Matrix Degradation by PI, PII and PIII Snake Venom Metalloproteinases: Clues on the Mechanisms of Venom-Induced Hemorrhage

    doi: 10.1371/journal.pntd.0003731

    Figure Lengend Snippet: Immunolocalization of SVMPs with vascular basement membrane on cremaster muscle ex vivo . Isolated cremaster muscles were incubated for 15 min with equi-hemorrhagic amounts of either BaP1 (PI, 30 μg), BlatH1 (PII, 3.5 μg) or CsH1 (PIII, 15 μg) SVMPs labeled with Alexa Fluor 647 (blue). Control tissues were incubated with the SVMPs without labeling and no fluorescence was detected. Whole tissues were fixed with 4% paraformaldehyde and immunostained with anti-collagen IV following the secondary antibody labeled with Alexa Fluor 488 (green). Tissues were visualized in a Zeiss LSM 5 Pascal laser-scanning confocal microscope. Three-dimensional reconstitution of the images and analysis of co-localization were carried out with the IMARIS x64 7.4.2 software as described in Methods. (A) Distribution of the SVMPs in the cremaster muscle tissue. Scale bar represents 150 μm. (B) White areas represent co-localization of the SVMPs (blue) with collagen IV (green) of vascular basement membrane in PCV, arterioles, and capillaries. Scale bar represents 20 μm. Results are expressed as the mean ± SEM of (C) percentage of material of SMVPs co-localized with collagen IV of vascular basement membrane, and (D) Pearson´s correlation coefficient of at least four vessels type per tissue (n = 3). *p

    Article Snippet: After three washes in PBS, tissues were incubated for 4 h at 4°C with goat anti-rabbit polyclonal antibody labeled with Alexa Fluor 488 at a dilution of 1:200 (Invitrogen A11034).

    Techniques: Ex Vivo, Isolation, Incubation, Labeling, Fluorescence, Microscopy, Software

    USF-1 interacts with the ILEI promoter sequence. A, 5′-biotin–tagged ILEI promoter oligonucleotide constructs with WT or mutant E-box. Biotin pulldown analysis of nuclear extracts from 501-Mel melanoma cells, followed by immunoblot for USF-1. B, PCR primers flanking the ILEI promoter E-box used in ChIP analysis. ChIP analysis of 501-Mel melanoma cell lines immunoprecipitated with control IgG or USF-1 antibody. PCR analysis conducted with primers targeting FAM3C, TYR, or HO1 promoter.

    Journal: The Journal of Biological Chemistry

    Article Title: Interleukin-like EMT inducer (ILEI) promotes melanoma invasiveness and is transcriptionally up-regulated by upstream stimulatory factor-1 (USF-1)

    doi: 10.1074/jbc.RA118.003616

    Figure Lengend Snippet: USF-1 interacts with the ILEI promoter sequence. A, 5′-biotin–tagged ILEI promoter oligonucleotide constructs with WT or mutant E-box. Biotin pulldown analysis of nuclear extracts from 501-Mel melanoma cells, followed by immunoblot for USF-1. B, PCR primers flanking the ILEI promoter E-box used in ChIP analysis. ChIP analysis of 501-Mel melanoma cell lines immunoprecipitated with control IgG or USF-1 antibody. PCR analysis conducted with primers targeting FAM3C, TYR, or HO1 promoter.

    Article Snippet: The following secondary antibodies were used: goat anti-mouse IgG (31430; ThermoFisher; 1:10,000) and goat anti-rabbit IgG3 (31460; ThermoFisher; 1:10,000).

    Techniques: Sequencing, Construct, Mutagenesis, Polymerase Chain Reaction, Chromatin Immunoprecipitation, Immunoprecipitation