Journal: Molecules

Article Title: Synthesis and In Vitro Anticancer Activity of Novel 4-Aryl-3-(4-methoxyphenyl)-1-phenyl-1 H -pyrazolo[3,4- b ]pyridines Arrest Cell Cycle and Induce Cell Apoptosis by Inhibiting CDK2 and/or CDK9

doi: 10.3390/molecules28176428

Figure Lengend Snippet: Apoptosis analysis for 9a and 14g against MCF7, HCT-116, and Hela cancer cell lines.

Article Snippet: An apoptotic study using the Annexin V-FITC Apoptosis Detection Kit (K101–25, BioVision ® , 980 Linda Vista Avenue, Mountain View, CA, USA) at their IC 50 concentration values was used on the specified cell after 24 h in three successive steps according to the manufacturer’s instructions.

Techniques:

Journal: Molecules

Article Title: Synthesis and In Vitro Anticancer Activity of Novel 4-Aryl-3-(4-methoxyphenyl)-1-phenyl-1 H -pyrazolo[3,4- b ]pyridines Arrest Cell Cycle and Induce Cell Apoptosis by Inhibiting CDK2 and/or CDK9

doi: 10.3390/molecules28176428

Figure Lengend Snippet: Representative diagram for apoptosis analyses for 9a against Hela and 14g against MCF7 and HCT-116, respectively.

Article Snippet: An apoptotic study using the Annexin V-FITC Apoptosis Detection Kit (K101–25, BioVision ® , 980 Linda Vista Avenue, Mountain View, CA, USA) at their IC 50 concentration values was used on the specified cell after 24 h in three successive steps according to the manufacturer’s instructions.

Techniques:

Journal: Molecules

Article Title: Synthesis and In Vitro Anticancer Activity of Novel 4-Aryl-3-(4-methoxyphenyl)-1-phenyl-1 H -pyrazolo[3,4- b ]pyridines Arrest Cell Cycle and Induce Cell Apoptosis by Inhibiting CDK2 and/or CDK9

doi: 10.3390/molecules28176428

Figure Lengend Snippet: Apoptosis analyses for 9a against Hela and 14g against MCF7 and HCT-116, respectively.

Article Snippet: An apoptotic study using the Annexin V-FITC Apoptosis Detection Kit (K101–25, BioVision ® , 980 Linda Vista Avenue, Mountain View, CA, USA) at their IC 50 concentration values was used on the specified cell after 24 h in three successive steps according to the manufacturer’s instructions.

Techniques:

Journal: Canadian Respiratory Journal

Article Title: Extracorporeal Shock Waves Increase Markers of Cellular Proliferation in Bronchial Epithelium and in Primary Bronchial Fibroblasts of COPD Patients

doi: 10.1155/2020/1524716

Figure Lengend Snippet: List of ELISA tests, cell proliferation, and protein extraction kits used. For ELISA tests, dilution of the supernatants or cell lysate samples used and detection limits are also reported.

Article Snippet: c-Kit or SCFR (CD117) , Cloud-Clone Corp. , SEA121 Hu , 1 : 5 (PBS) , 0.61 ng/mL (1.56–100 ng/mL).

Techniques: Enzyme-linked Immunosorbent Assay, Protein Extraction

Journal: Canadian Respiratory Journal

Article Title: Extracorporeal Shock Waves Increase Markers of Cellular Proliferation in Bronchial Epithelium and in Primary Bronchial Fibroblasts of COPD Patients

doi: 10.1155/2020/1524716

Figure Lengend Snippet: C-Kit (CD117) mRNA (a, b, c) and protein (d, e, f) expression after ESW treatment in primary bronchial fibroblasts of COPD patients (a, d), primary bronchial fibroblasts of control smokers (b, e), and bronchial epithelial cells (c, f). In bronchial epithelium (16HBE) c-Kit increased at mRNA (c) and protein (f) levels. In primary bronchial fibroblasts of COPD patients, c-Kit increased at protein level (d). T -test was used for comparative purposes, and p values are reported in the graphs.

Article Snippet: c-Kit or SCFR (CD117) , Cloud-Clone Corp. , SEA121 Hu , 1 : 5 (PBS) , 0.61 ng/mL (1.56–100 ng/mL).

Techniques: Expressing

Journal: Canadian Respiratory Journal

Article Title: Extracorporeal Shock Waves Increase Markers of Cellular Proliferation in Bronchial Epithelium and in Primary Bronchial Fibroblasts of COPD Patients

doi: 10.1155/2020/1524716

Figure Lengend Snippet: Photomicrographs showing thyroid transcription factor-1 (TTF-1) expression (panels a, b), c-Kit (CD117) (c, d), and proliferating cell nuclear antigen (PCNA) (e, f) in the peripheral lung tissue of a representative patient with chronic obstructive pulmonary disease (COPD). Arrows indicate positively stained cells mainly located in the alveolar septa. Bars = 50 microns.

Article Snippet: c-Kit or SCFR (CD117) , Cloud-Clone Corp. , SEA121 Hu , 1 : 5 (PBS) , 0.61 ng/mL (1.56–100 ng/mL).

Techniques: Expressing, Staining

Journal: Canadian Respiratory Journal

Article Title: Extracorporeal Shock Waves Increase Markers of Cellular Proliferation in Bronchial Epithelium and in Primary Bronchial Fibroblasts of COPD Patients

doi: 10.1155/2020/1524716

Figure Lengend Snippet: Photomicrographs showing alveolar type II epithelial cells (TTF-1+ cells, red color) coexpressing c-Kit (CD117) (brown color) (a, b) and PCNA (brown color) (c, d) in the peripheral lung tissue of a representative patient with COPD. Positive double-stained cells can be recognized in the alveolar septa, even though their presence was only rarely observed. Arrows indicate positively stained cells located in the alveolar septa. Bars = 50 microns.

Article Snippet: c-Kit or SCFR (CD117) , Cloud-Clone Corp. , SEA121 Hu , 1 : 5 (PBS) , 0.61 ng/mL (1.56–100 ng/mL).

Techniques: Staining

Journal: Disease Markers

Article Title: Evaluation of Immune Infiltration Based on Image Plus Helps Predict the Prognosis of Stage III Gastric Cancer Patients with Significantly Different Outcomes in Northeastern China

doi: 10.1155/2022/2893336

Figure Lengend Snippet: Expression of CD4, CD8, CD20, CD56, CD68, CD117, and CD177 by immune cells infiltrated tumor tissues of GC patients. Representative images of immune markers staining in immune cells from GC samples are shown at ×200 (100 mm) original magnification. Positive expression of CD4 (a), CD8 (b), CD20 (c), CD56 (d), CD68 (e), CD117 (f), and CD177 (g) infiltrated in tumor tissues with a regular distribution. In order to show the distribution from immune cells more clearly, we used computerized imaging system Image-Pro Plus version 6.2 to highlight positive areas in different colors.

Article Snippet: After cleaned in distilled water, the paraffin sections were pretreated with citrate buffer, pH 6.0 (CD177) and EDTA Antigen Retrieval Solution, pH 8.0 (CD4, CD8, CD20, CD56, CD68, and CD117) for 3 min at 120°C in a pressure cooker, and endogenous peroxidase was inhibited with 3% H 2 O 2 in PBS for 10 min. Nonspecific actions in the sections were also blocked with goat serum (BOSTER, USA) for 1 h at room temperature.

Techniques: Expressing, Staining, Imaging

Journal: Disease Markers

Article Title: Evaluation of Immune Infiltration Based on Image Plus Helps Predict the Prognosis of Stage III Gastric Cancer Patients with Significantly Different Outcomes in Northeastern China

doi: 10.1155/2022/2893336

Figure Lengend Snippet: The schematic diagram of the distribution of immune cells. The blue points represent CD20 + B cells, the yellow points represent CD4 + T cells, the orange points represent CD117 + mast cells, the green points represent CD68 + macrophages, the pink points represent CD8 + T cells, the black points represent CD56 + NK cells, and the red points represent CD177 + neutrophils. Although not all immune cells are distributed according to this fixed law, this pattern can basically reflect the general characteristics of their distribution.

Article Snippet: After cleaned in distilled water, the paraffin sections were pretreated with citrate buffer, pH 6.0 (CD177) and EDTA Antigen Retrieval Solution, pH 8.0 (CD4, CD8, CD20, CD56, CD68, and CD117) for 3 min at 120°C in a pressure cooker, and endogenous peroxidase was inhibited with 3% H 2 O 2 in PBS for 10 min. Nonspecific actions in the sections were also blocked with goat serum (BOSTER, USA) for 1 h at room temperature.

Techniques:

Journal: Disease Markers

Article Title: Evaluation of Immune Infiltration Based on Image Plus Helps Predict the Prognosis of Stage III Gastric Cancer Patients with Significantly Different Outcomes in Northeastern China

doi: 10.1155/2022/2893336

Figure Lengend Snippet: Differences in immune marker positive area/total area between the two groups (survival time of patients in group A was less than 1 year and group B survival time was more than 5 years) by the rank sum test. (a) CD4 + T cells ( P < 0.001). (b) CD8 + T cells ( P < 0.001). (c) CD20 + B cells ( P < 0.001). (d) CD68 + macrophages ( P < 0.001). (e) CD117 + mast cells ( P < 0.001). (f) CD177 + neutrophils ( P < 0.001).

Article Snippet: After cleaned in distilled water, the paraffin sections were pretreated with citrate buffer, pH 6.0 (CD177) and EDTA Antigen Retrieval Solution, pH 8.0 (CD4, CD8, CD20, CD56, CD68, and CD117) for 3 min at 120°C in a pressure cooker, and endogenous peroxidase was inhibited with 3% H 2 O 2 in PBS for 10 min. Nonspecific actions in the sections were also blocked with goat serum (BOSTER, USA) for 1 h at room temperature.

Techniques: Marker