Journal: The Journal of Investigative Dermatology

Article Title: Mosaic Activating Mutations in GNA11 and GNAQ Are Associated with Phakomatosis Pigmentovascularis and Extensive Dermal Melanocytosis

doi: 10.1016/j.jid.2015.11.027

Figure Lengend Snippet: Clinical phenotype and GNA11 and GNAQ genotypes with percentage mosaicism (where available) of different samples from eight patients with PPV (patients 1–8) and three patients with extensive dermal melanocytosis with no vascular phenotype (patients 9–11), showing postzygotic mosaicism

Article Snippet: The human GNA11 cDNA clone was purchased from Origene (purchased from NM_002067.1 precloned into an untagged pCMV6-XL4 vector, catalog number SC303115).

Techniques: Biomarker Discovery, Mutagenesis

Journal: The Journal of Investigative Dermatology

Article Title: Mosaic Activating Mutations in GNA11 and GNAQ Are Associated with Phakomatosis Pigmentovascularis and Extensive Dermal Melanocytosis

doi: 10.1016/j.jid.2015.11.027

Figure Lengend Snippet: Sequencing results demonstrating mosaic GNA11 and GNAQ mutations. ( a ) Sanger sequencing of skin biopsy showing a very low peak in GNA11 at position c.547C>T (p.Arg183Cys) (asterisk). ( b ) Sanger sequencing of the same skin biopsy deoxyribonucleic acid after restriction enzyme digest of the normal allele, and hemi-nested amplification, revealing the mutation (asterisk). ( c ) Targeted next-generation sequencing showing low allele percentage mutations in skin but undetectable in blood, GNA11 c.547C>T (p.Arg183Cys) in 5% of reads. ( d ) GNAQ c.548G>A (p.Arg183Gln) in 6% of reads from skin but undetectable in blood.

Article Snippet: The human GNA11 cDNA clone was purchased from Origene (purchased from NM_002067.1 precloned into an untagged pCMV6-XL4 vector, catalog number SC303115).

Techniques: Sequencing, Amplification, Mutagenesis, Next-Generation Sequencing

Journal: The Journal of Investigative Dermatology

Article Title: Mosaic Activating Mutations in GNA11 and GNAQ Are Associated with Phakomatosis Pigmentovascularis and Extensive Dermal Melanocytosis

doi: 10.1016/j.jid.2015.11.027

Figure Lengend Snippet: Mutant GNA11 leads to activation of downstream signaling pathways. ( a ) Western blot for Flag, total GNA11, and the phosphorylated and total p38, JNK, ERK and AKT in total protein lysates from HEK293T cells transfected with either vector, wild-type GNA11 (WT), or one of two mutants R183C or Q209L mutant GNA11 . ( b ) Ratio of phosphorylated to total p38, JNK, ERK, and AKT normalized by actin loading control. Bars indicate 1 standard deviation. ∗ P < 0.05, unpaired t-test.

Article Snippet: The human GNA11 cDNA clone was purchased from Origene (purchased from NM_002067.1 precloned into an untagged pCMV6-XL4 vector, catalog number SC303115).

Techniques: Mutagenesis, Activation Assay, Protein-Protein interactions, Western Blot, Transfection, Plasmid Preparation, Control, Standard Deviation

Journal: The Journal of Investigative Dermatology

Article Title: Mosaic Activating Mutations in GNA11 and GNAQ Are Associated with Phakomatosis Pigmentovascularis and Extensive Dermal Melanocytosis

doi: 10.1016/j.jid.2015.11.027

Figure Lengend Snippet: Mosaic expression of GNA11 promotes ectopic pigmentary lesions in zebrafish. ( a ) Images of adult zebrafish mosaic for GNA11, GNA11 R183C , or GNA11 Q209L expression. Large, ectopic pigmentary lesions are indicated next to white arrows. Dashed box indicates zoomed areas that show detail of pigmentary lesions. ( b ) Numbers of pigmentary lesions per fish expressing GNA11, GNA11 R183C , or GNA11 Q209L . Dark circles indicate ectopic pigmentary lesions. White circles indicate fish without pigmentary lesions. ( c ) Histology hematoxylin and eosin staining of a wild-type and GNA11 R183C zebrafish skin at ×100 and ×400 magnification. Melanocytes are clearly visible in the dermis by the black melanin (blue arrows), frequently also in the epidermis (not shown) and in a few cases within underlying muscle (yellow arrow).

Article Snippet: The human GNA11 cDNA clone was purchased from Origene (purchased from NM_002067.1 precloned into an untagged pCMV6-XL4 vector, catalog number SC303115).

Techniques: Expressing, Staining

Journal: Immunity

Article Title: Vaccination Induces Maturation in a Mouse Model of Diverse Unmutated VRC01-Class Precursors to HIV-Neutralizing Antibodies with >50% Breadth

doi: 10.1016/j.immuni.2020.12.014

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: The untagged Env immunogens, including eODGT6(8) 60mers, gp120 cores, and gp120 core ferritin nanoparticles, were purified with Galanthus nivalis (GNA)-lectin gel (EY Laboratories, Inc.) and followed with a size exclusion chromatography (SEC).

Techniques: Reverse Transcription, Staining, Sequencing, Expressing, Plasmid Preparation, Software

Journal: Immunity

Article Title: Vaccination Induces Maturation in a Mouse Model of Diverse Unmutated VRC01-Class Precursors to HIV-Neutralizing Antibodies with >50% Breadth

doi: 10.1016/j.immuni.2020.12.014

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: The untagged Env immunogens, including eODGT6(8) 60mers, gp120 cores, and gp120 core ferritin nanoparticles, were purified with Galanthus nivalis (GNA)-lectin gel (EY Laboratories, Inc.) and followed with a size exclusion chromatography (SEC).

Techniques: Reverse Transcription, Staining, Sequencing, Expressing, Plasmid Preparation, Software