Journal: Translational Psychiatry

Article Title: Synergistic inhibition of histone modifiers produces therapeutic effects in adult Shank3 -deficient mice

doi: 10.1038/s41398-021-01233-w

Figure Lengend Snippet: A , B Bar graphs showing mRNA levels of NMDAR subunits ( Grin1, Grin2a, Grin2b ) and AMPAR ( Gria1, Gria2 ) subunits ( A ) and synaptic proteins ( Shank3 , Homber1b , Arc ) ( B ) in PFC of adult saline-treated WT or Shank3 +/ΔC mice treated with saline or Rom/GSK (0.25/5 mg/kg, i.p., 3x) at 8 days post-treatment. n = 7–11 mice per group. C, D Bar graphs showing total ( C ) or synaptic ( D ) protein levels of NMDAR subunits in the 3 groups at 8 days post-treatment. C, n = 7–9 mice/group; D, n = 5–7 mice per group. Insets: representative immunoblots. In all figures, # p < 0.1, * p < 0.05, **p < 0.01, *** p < 0.001.

Article Snippet: Western blots were performed using antibodies against NR1 (1:500, NeuroMab, 75‐ 272), NR2A (1:500, Millipore, 07‐632), NR2B (1:500, Millipore, 06‐600), PSD95 (1:1000, Cell Signaling, 36233 S) and tubulin (1:5000 Sigma, T9026).

Techniques: Saline, Western Blot

Journal: Neuron

Article Title: Regulation of Thalamic and Cortical Network Synchrony by Scn8a

doi: 10.1016/j.neuron.2017.01.031

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Monoclonal mouse anti-GluR2 , Neuromab , Cat# 75-002; RRID: AB_2232661.

Techniques: Plasmid Preparation, Virus, Recombinant, Avidin-Biotin Assay, Software, Imaging

Journal: Heliyon

Article Title: Identification and validation of a disulfidptosis-related genes prognostic signature in lung adenocarcinoma

doi: 10.1016/j.heliyon.2023.e23502

Figure Lengend Snippet: Evaluation of SLC7A11, SLC3A2, NCKAP1 and GYS1 expression and prognosis value in LUAD. (A–D) Immunohistochemical staining of NCKAP1, GYS1, SLC7A11, SLC3A2 in clinical LUAD samples between tumor and adjacent tissues (scale bars, 20 μm). (E) Immunoblot of NCKAP1 in lung cancer cells. CCK-8 assay results showed the NCKAP1 knockdown cell proliferation in H460 cell lines, quantification of cell death after NCKAP1 knockdown by flow cytometry. (F) Immunoblot analysis of GYS1 in lung cancer cells. CCK-8 assay about GYS1 knockdown cell proliferation in H460 cell lines, quantification of cell death after NCKAP1 knockdown by flow cytometry. (G) NCKAP1 inhibited cell growth, migration and invasion in H460. Cell colony formation; wound-healing assay: Si -NCKAP1-1 and Si -NCKAP1-2; transwell invasion showed a decrease of invaded knockdown cells. Scale bar = 20 μm. (H) GYS1 inhibited cell growth, migration and invasion in H460. Cell colony formation; wound-healing: Si -GYS1-1 and Si -GYS1-2; transwell invasion assay results showed a decrease of invaded knockdown cells. Scale bar = 20 μm. (I) Correlation between GYS1 expression level and infiltration level of CSF1, quantitative analysis of CSF1 using real time PCR in GYS1 high and low expression groups. (J) IHC staining of GYS1 high/low expression and CD163 infiltration in LUAD tissue, scale bar = 20 μm.

Article Snippet: As previously described [ ], the paraffin-embedded microarray tumor tissues using IHC primary antibodies, including SLC7A11 (Proteintech 26864-1-AP), SLC3A2 (Proteintech 15193-1-AP), NCKAP1 (Proteintech, 12140-1-AP), and GYS1 (Proteintech 10566-1-AP) antibodies, along with their corresponding non-cancerous tissues.

Techniques: Expressing, Immunohistochemical staining, Staining, Western Blot, CCK-8 Assay, Knockdown, Flow Cytometry, Migration, Wound Healing Assay, Transwell Invasion Assay, Real-time Polymerase Chain Reaction, Immunohistochemistry