glun2b blocking peptide Search Results


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    Cell Signaling Technology Inc rabbit polyclonal anti phospho nr2b tyr1472 antibody
    Changes in <t>NR2B</t> subunit phosphorylation state at <t>Tyr1472</t> in homogenates and the P3 fraction. (A) Change in the NR2B phosphorylation state at Tyr1472 in homogenates. The membrane was stripped and reprobed with anti-NR2B antibody. The graph summarizes the percentage changes in NR2B-Y1472 p in homogenates. n = 6 experiments per group. (B) Change in phosphorylation state of NR2B at Tyr1472 in the P3 fraction. The membrane was stripped and reprobed with anti-NR2B antibody. The graph summarizes the percentage change in NR2B-Y1472 p in the P3 fraction. * p
    Rabbit Polyclonal Anti Phospho Nr2b Tyr1472 Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit polyclonal anti phospho nr2b tyr1472 antibody/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit polyclonal anti phospho nr2b tyr1472 antibody - by Bioz Stars, 2022-10
    94/100 stars
      Buy from Supplier

    95
    Cell Signaling Technology Inc anti glun2b rabbit polyclonal antibody
    Effects of <t>NMDAR1</t> antagonists memantine ( a,b ) and MK-801 ( c,d ) and NMDAR2B antagonist Ifenprodil ( e,f ) on H345 ( a,c,e ) and H82 cell ( b,d,f ) proliferation after 24 h (●), 48 h (■) and 72 h (Δ) of treatment. NMDAR1 and NMDAR2 receptors appear to be important for proliferation of SCLC culture cell lines. Percentage reduction in cell viability produced in NCI H345 classical cells ( a,c,e ) and in NCI H82 variant cells ( b,d,f ) by the action of irreversible NMDAR1 receptor antagonist Memantine (a,b), irreversible NMDAR1 receptor antagonist Dizocilpine Maleate ( c,d ), and irreversible NMDAR2B receptor antagonist Ifenprodil ( e,f ) following 24 h (●), 48 h (■) and 72 h (Δ) of treatment. Cell viability was reduced to about 10% of control as assessed using Alamar Blue staining. Reductions in viability were all highly significant (p
    Anti Glun2b Rabbit Polyclonal Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti glun2b rabbit polyclonal antibody/product/Cell Signaling Technology Inc
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti glun2b rabbit polyclonal antibody - by Bioz Stars, 2022-10
    95/100 stars
      Buy from Supplier

    95
    Cell Signaling Technology Inc nmda receptor 2b glun2b d15b3 rabbit mab
    Effects of <t>NMDAR1</t> antagonists memantine ( a,b ) and MK-801 ( c,d ) and NMDAR2B antagonist Ifenprodil ( e,f ) on H345 ( a,c,e ) and H82 cell ( b,d,f ) proliferation after 24 h (●), 48 h (■) and 72 h (Δ) of treatment. NMDAR1 and NMDAR2 receptors appear to be important for proliferation of SCLC culture cell lines. Percentage reduction in cell viability produced in NCI H345 classical cells ( a,c,e ) and in NCI H82 variant cells ( b,d,f ) by the action of irreversible NMDAR1 receptor antagonist Memantine (a,b), irreversible NMDAR1 receptor antagonist Dizocilpine Maleate ( c,d ), and irreversible NMDAR2B receptor antagonist Ifenprodil ( e,f ) following 24 h (●), 48 h (■) and 72 h (Δ) of treatment. Cell viability was reduced to about 10% of control as assessed using Alamar Blue staining. Reductions in viability were all highly significant (p
    Nmda Receptor 2b Glun2b D15b3 Rabbit Mab, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nmda receptor 2b glun2b d15b3 rabbit mab/product/Cell Signaling Technology Inc
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    nmda receptor 2b glun2b d15b3 rabbit mab - by Bioz Stars, 2022-10
    95/100 stars
      Buy from Supplier

    Image Search Results


    Changes in NR2B subunit phosphorylation state at Tyr1472 in homogenates and the P3 fraction. (A) Change in the NR2B phosphorylation state at Tyr1472 in homogenates. The membrane was stripped and reprobed with anti-NR2B antibody. The graph summarizes the percentage changes in NR2B-Y1472 p in homogenates. n = 6 experiments per group. (B) Change in phosphorylation state of NR2B at Tyr1472 in the P3 fraction. The membrane was stripped and reprobed with anti-NR2B antibody. The graph summarizes the percentage change in NR2B-Y1472 p in the P3 fraction. * p

    Journal: PLoS ONE

    Article Title: The spinal NR2BR/ERK2 pathway as a target for the central sensitization of collagen-induced arthritis pain

    doi: 10.1371/journal.pone.0201021

    Figure Lengend Snippet: Changes in NR2B subunit phosphorylation state at Tyr1472 in homogenates and the P3 fraction. (A) Change in the NR2B phosphorylation state at Tyr1472 in homogenates. The membrane was stripped and reprobed with anti-NR2B antibody. The graph summarizes the percentage changes in NR2B-Y1472 p in homogenates. n = 6 experiments per group. (B) Change in phosphorylation state of NR2B at Tyr1472 in the P3 fraction. The membrane was stripped and reprobed with anti-NR2B antibody. The graph summarizes the percentage change in NR2B-Y1472 p in the P3 fraction. * p

    Article Snippet: After blocking with 5% skim milk in PBST for 1 h, the membranes were incubated overnight at 4°C with primary rabbit polyclonal anti-NR2B antibody (1:1,000; Merck, Germany), mouse monoclonal anti-NR1 antibody (1:1,000; BD Pharmigen, USA), rabbit monoclonal anti-NR2A antibody (1:1,000; Abcam, USA), rabbit polyclonal anti-Phospho-NR2B (Tyr1472) antibody (1:1,000; Cell Signaling Technology, USA), rabbit polyclonal anti-β-actin antibody (1:1,000; ImmunoWay, USA), mouse monoclonal anti-Phospho-ERK1/2 (Thr202/Tyr204) antibody, and rabbit polyclonal anti-ERK1/2 antibody (1:1,000; Cell Signaling Technology, USA).

    Techniques:

    Changes in total protein expression of NR1, NR2B, and NR2A subunits, and NR2B phosphorylation level at Tyr1472, in homogenates. (A) The immunoreactivity changes in NR1, NR2B, and NR2A subunits in homogenates are shown. The homogenates were prepared 30 min after drug treatment. The graph summarizes the percentage changes in immunoreactivity of NR1, NR2B, and NR2A. Equal protein loadings are indicated by β-actin. * p

    Journal: PLoS ONE

    Article Title: The spinal NR2BR/ERK2 pathway as a target for the central sensitization of collagen-induced arthritis pain

    doi: 10.1371/journal.pone.0201021

    Figure Lengend Snippet: Changes in total protein expression of NR1, NR2B, and NR2A subunits, and NR2B phosphorylation level at Tyr1472, in homogenates. (A) The immunoreactivity changes in NR1, NR2B, and NR2A subunits in homogenates are shown. The homogenates were prepared 30 min after drug treatment. The graph summarizes the percentage changes in immunoreactivity of NR1, NR2B, and NR2A. Equal protein loadings are indicated by β-actin. * p

    Article Snippet: After blocking with 5% skim milk in PBST for 1 h, the membranes were incubated overnight at 4°C with primary rabbit polyclonal anti-NR2B antibody (1:1,000; Merck, Germany), mouse monoclonal anti-NR1 antibody (1:1,000; BD Pharmigen, USA), rabbit monoclonal anti-NR2A antibody (1:1,000; Abcam, USA), rabbit polyclonal anti-Phospho-NR2B (Tyr1472) antibody (1:1,000; Cell Signaling Technology, USA), rabbit polyclonal anti-β-actin antibody (1:1,000; ImmunoWay, USA), mouse monoclonal anti-Phospho-ERK1/2 (Thr202/Tyr204) antibody, and rabbit polyclonal anti-ERK1/2 antibody (1:1,000; Cell Signaling Technology, USA).

    Techniques: Expressing

    Effects of NMDAR1 antagonists memantine ( a,b ) and MK-801 ( c,d ) and NMDAR2B antagonist Ifenprodil ( e,f ) on H345 ( a,c,e ) and H82 cell ( b,d,f ) proliferation after 24 h (●), 48 h (■) and 72 h (Δ) of treatment. NMDAR1 and NMDAR2 receptors appear to be important for proliferation of SCLC culture cell lines. Percentage reduction in cell viability produced in NCI H345 classical cells ( a,c,e ) and in NCI H82 variant cells ( b,d,f ) by the action of irreversible NMDAR1 receptor antagonist Memantine (a,b), irreversible NMDAR1 receptor antagonist Dizocilpine Maleate ( c,d ), and irreversible NMDAR2B receptor antagonist Ifenprodil ( e,f ) following 24 h (●), 48 h (■) and 72 h (Δ) of treatment. Cell viability was reduced to about 10% of control as assessed using Alamar Blue staining. Reductions in viability were all highly significant (p

    Journal: Clinical pharmacology : advances and applications

    Article Title: NMDA receptors are expressed by small-cell lung cancer and are potential targets for effective treatment

    doi:

    Figure Lengend Snippet: Effects of NMDAR1 antagonists memantine ( a,b ) and MK-801 ( c,d ) and NMDAR2B antagonist Ifenprodil ( e,f ) on H345 ( a,c,e ) and H82 cell ( b,d,f ) proliferation after 24 h (●), 48 h (■) and 72 h (Δ) of treatment. NMDAR1 and NMDAR2 receptors appear to be important for proliferation of SCLC culture cell lines. Percentage reduction in cell viability produced in NCI H345 classical cells ( a,c,e ) and in NCI H82 variant cells ( b,d,f ) by the action of irreversible NMDAR1 receptor antagonist Memantine (a,b), irreversible NMDAR1 receptor antagonist Dizocilpine Maleate ( c,d ), and irreversible NMDAR2B receptor antagonist Ifenprodil ( e,f ) following 24 h (●), 48 h (■) and 72 h (Δ) of treatment. Cell viability was reduced to about 10% of control as assessed using Alamar Blue staining. Reductions in viability were all highly significant (p

    Article Snippet: Most Western blotting involved the NMDAR1 antibody from Cell Signaling Technology because this commercial product worked better than our PANN1 antibodies with this procedure, while PANN1 proved to be superior for immunohistochemistry.

    Techniques: Produced, Variant Assay, Staining

    Genes for both NMDAR1 and NMDAR2B receptors are expressed in cultured small-cell lung cancer cells. A ) The expression of NMDAR1 receptors in the cultured cell lines by RT-PCR using primers designed to amplify different regions of the coding sequence (see text for details). Shown are results obtained for classical cell lines NCI H345, NCI H146, and DMS 53, and variant cell line NCI H82. B ) The expression of NMDAR2B receptors by cultured cell lines by RT-PCR using primers designed to amplify different regions of the coding sequence for this receptor (see text for details). Shown are results obtained for classical cell lines NCI 146 and DMS 53, and variant cell line NCI H82. Sequence analysis of the products using nested primers indicated normal NMDAR1 and NMDAR2B messages. Products were separated on 1.5% agarose gels, which were then stained with ethidium bromide. In each case one band of the expected size was obtained for the amplification product.

    Journal: Clinical pharmacology : advances and applications

    Article Title: NMDA receptors are expressed by small-cell lung cancer and are potential targets for effective treatment

    doi:

    Figure Lengend Snippet: Genes for both NMDAR1 and NMDAR2B receptors are expressed in cultured small-cell lung cancer cells. A ) The expression of NMDAR1 receptors in the cultured cell lines by RT-PCR using primers designed to amplify different regions of the coding sequence (see text for details). Shown are results obtained for classical cell lines NCI H345, NCI H146, and DMS 53, and variant cell line NCI H82. B ) The expression of NMDAR2B receptors by cultured cell lines by RT-PCR using primers designed to amplify different regions of the coding sequence for this receptor (see text for details). Shown are results obtained for classical cell lines NCI 146 and DMS 53, and variant cell line NCI H82. Sequence analysis of the products using nested primers indicated normal NMDAR1 and NMDAR2B messages. Products were separated on 1.5% agarose gels, which were then stained with ethidium bromide. In each case one band of the expected size was obtained for the amplification product.

    Article Snippet: Most Western blotting involved the NMDAR1 antibody from Cell Signaling Technology because this commercial product worked better than our PANN1 antibodies with this procedure, while PANN1 proved to be superior for immunohistochemistry.

    Techniques: Cell Culture, Expressing, Reverse Transcription Polymerase Chain Reaction, Sequencing, Variant Assay, Staining, Amplification

    IHC analysis of SCLC tumor: A ) Positive staining with polyclonal antibody and B ) Negative control with polyclonal antibody + blocking p eptides. NMDAR1 protein is present in small-cell lung tumors. A ) Positive ABC immunohistochemical staining in a tissue section of a primary tumor located in the lung of a patient using our polyclonal antibodies that recognize an N-terminal peptide fragment of the receptor. Staining was concentrated at the surface of cancer cells and absent from adjacent normal cells. B ) Control ‘peptide-negative’ ABC immunohistochemical staining in an adjacent section when the polyclonal antibodies were masked by addition of excess peptide antigen (magnification 20x).

    Journal: Clinical pharmacology : advances and applications

    Article Title: NMDA receptors are expressed by small-cell lung cancer and are potential targets for effective treatment

    doi:

    Figure Lengend Snippet: IHC analysis of SCLC tumor: A ) Positive staining with polyclonal antibody and B ) Negative control with polyclonal antibody + blocking p eptides. NMDAR1 protein is present in small-cell lung tumors. A ) Positive ABC immunohistochemical staining in a tissue section of a primary tumor located in the lung of a patient using our polyclonal antibodies that recognize an N-terminal peptide fragment of the receptor. Staining was concentrated at the surface of cancer cells and absent from adjacent normal cells. B ) Control ‘peptide-negative’ ABC immunohistochemical staining in an adjacent section when the polyclonal antibodies were masked by addition of excess peptide antigen (magnification 20x).

    Article Snippet: Most Western blotting involved the NMDAR1 antibody from Cell Signaling Technology because this commercial product worked better than our PANN1 antibodies with this procedure, while PANN1 proved to be superior for immunohistochemistry.

    Techniques: Immunohistochemistry, Staining, Negative Control, Blocking Assay

    Western analysis of NMDA receptor proteins in A ) NCI H345 and B ) NCI H82 SCLC cells. NMDAR receptor proteins are present in SCLC cancer cell lysates from all four cell lines. Lysates were analyzed by SDS-PAGE using 12.5% gels and Western blot using our own and commercial antibody preparations recognizing NMDAR1 proteins. Shown are results obtained for NCI H345 A ) and NCI H82 B ) cells with a commercial antibody against NMDAR1 protein (Cell Signaling Corp). Molecular mass markers (kDa) are indicated on the right side of the figure and demonstrate the presence in lysates of a major form protein of 116 kDa and a minor form of 55 kDa.

    Journal: Clinical pharmacology : advances and applications

    Article Title: NMDA receptors are expressed by small-cell lung cancer and are potential targets for effective treatment

    doi:

    Figure Lengend Snippet: Western analysis of NMDA receptor proteins in A ) NCI H345 and B ) NCI H82 SCLC cells. NMDAR receptor proteins are present in SCLC cancer cell lysates from all four cell lines. Lysates were analyzed by SDS-PAGE using 12.5% gels and Western blot using our own and commercial antibody preparations recognizing NMDAR1 proteins. Shown are results obtained for NCI H345 A ) and NCI H82 B ) cells with a commercial antibody against NMDAR1 protein (Cell Signaling Corp). Molecular mass markers (kDa) are indicated on the right side of the figure and demonstrate the presence in lysates of a major form protein of 116 kDa and a minor form of 55 kDa.

    Article Snippet: Most Western blotting involved the NMDAR1 antibody from Cell Signaling Technology because this commercial product worked better than our PANN1 antibodies with this procedure, while PANN1 proved to be superior for immunohistochemistry.

    Techniques: Western Blot, SDS Page

    NMDAR1 receptors have an influence on the growth of SCLC tumor xenografts in nu/nu mice. A ) Administration of MK-801 in doses up to 0.3 mg/kg twice daily has no impact on the health of nu/nu mice as assessed by body weight over and beyond the treatment period. B ) Growth of small-cell tumors from NCI-H345 cells is significantly (p

    Journal: Clinical pharmacology : advances and applications

    Article Title: NMDA receptors are expressed by small-cell lung cancer and are potential targets for effective treatment

    doi:

    Figure Lengend Snippet: NMDAR1 receptors have an influence on the growth of SCLC tumor xenografts in nu/nu mice. A ) Administration of MK-801 in doses up to 0.3 mg/kg twice daily has no impact on the health of nu/nu mice as assessed by body weight over and beyond the treatment period. B ) Growth of small-cell tumors from NCI-H345 cells is significantly (p

    Article Snippet: Most Western blotting involved the NMDAR1 antibody from Cell Signaling Technology because this commercial product worked better than our PANN1 antibodies with this procedure, while PANN1 proved to be superior for immunohistochemistry.

    Techniques: Mouse Assay