Journal: Diabetes

Article Title: Perivascular Adipose Tissue Control of Insulin-Induced Vasoreactivity in Muscle Is Impaired in db / db Mice

doi: 10.2337/db11-1603

Figure Lengend Snippet: The effects of C57BL/6 and db / db PVAT on basal tone, acetylcholine-induced vasoreactivity, insulin-induced vasoreactivity, and the insulin-signaling pathway, as well as the amount of adiponectin secretion. A : PVAT from either C57BL/6 or db / db mice does not affect smooth muscle tone of isolated resistance arteries (RAs) (white bar, tone in the absence of PVAT [ n = 6]; black bar, tone in the presence of C57BL/6 PVAT [ n = 5]; hatched bar, tone in the presence of db / db PVAT [ n = 7]). DMax, maximal diameter. B : PVAT from either C57BL/6 or db / db mice does not affect acetylcholine (ACh) 10 −7 –induced vasodilation, indicating that there is no general microvascular dysfunction (white bar, acetylcholine-dependent vasodilation without PVAT [ n = 5]; black bar, acetylcholine-dependent vasodilation in the presence of C57BL/6 PVAT [ n = 4]; hatched bar, acetylcholine-dependent vasodilation in the presence of db / db PVAT [ n = 6]). C : Insulin-induced vasoreactivity in the absence and presence of PVAT from control mice. C57BL/6-PVAT unveils insulin-induced vasodilation, which is not present in the absence of PVAT (white squares, C57BL/6 RA, without PVAT [ n = 9]; black squares, C57BL/6 RA in the presence of C57BL/6 PVAT [ n = 10]). * P < 0.05. D : Insulin-induced vasoreactivity in the presence of PVAT from C57BL/6 or db / db mice. PVAT from db / db mice does not unveil the vasodilator properties of insulin as PVAT from control mice does (black squares, C57BL/6 RA in the presence of C57BL/6 PVAT [ n = 10]; white circles, C57BL/6 RA in the presence of db / db PVAT [ n = 8]). * P < 0.05. E : Western blot of Akt phosphorylation initiated by insulin in RAs in the presence of C57BL/6 PVAT or db / db PVAT. Insulin causes a more pronounced Akt phosphorylation in the presence of C57BL/6 PVAT than in the presence of db / db PVAT. * P < 0.05 compared with no insulin; # P < 0.05 compared with C57Bl/6 PVAT.

Article Snippet: A soluble receptor fragment (Acrp-30 [ N -20]-P adiponectin receptor blocking peptide; Santa Cruz Biotechnology) (0.4 μg/mL), which binds adiponectin, was preincubated in the pressure myograph for 30 min. Additionally, globular adiponectin (Enzo Life Sciences, Antwerpen, Belgium) was preincubated for 45 min in the vessel bath of the pressure myograph at a concentration of 1 μg/mL with C57BL/6 resistance arteries without PVAT ( n = 5) to investigate whether adiponectin can affect insulin-induced vasoreactivity when added from outside the resistance artery.

Techniques: Isolation, Western Blot

Journal: Life

Article Title: A Longitudinal Study of the Relationship of Adiponectin with Reproduction in Infertile Women Undergoing IVF/ICSI Treatment, and an Experimental Study in Human Granulosa Cells

doi: 10.3390/life13040994

Figure Lengend Snippet: Comparisons of serum adiponectin levels between successful and unsuccessful pregnancies in various phases, as well as FF adiponectin levels in Phase III of the IVF/ICSI treatment.

Article Snippet: After the fourth passage, the KGN cells were incubated with serum-free DMEM/F-12 medium as the control; 10 −8 M FSH (Abcam, Cambridge, UK); 10 −8 M insulin-like growth factor-1 (IGF-1) (Sigma-Aldrich, St. Louis, MO, USA); 10 −8 M FSH together with 10 −8 M IGF-1 (FSH+IGF-1); 300 nM of globular adiponectin (Adipo) (Merck KGaA, Darmstadt, Germany); and 300 nM of adiponectin together with 10 −8 M FSH and 10 −8 M IGF-1 (Adipo+FSH+IGF-1).

Techniques:

Journal: Life

Article Title: A Longitudinal Study of the Relationship of Adiponectin with Reproduction in Infertile Women Undergoing IVF/ICSI Treatment, and an Experimental Study in Human Granulosa Cells

doi: 10.3390/life13040994

Figure Lengend Snippet: Comparisons of serum adiponectin levels among various phases, as well as between serum and follicular fluid (FF) in total ( n = 29), successful ( n = 10), and unsuccessful groups ( n = 19) in Phase III of the in vitro fertilization (IVF)/intracytoplasmic sperm injection (ICSI) treatment. ( a ) Comparisons of serum adiponectin levels among the various phases. ( b ) Comparisons of adiponectin levels between serum and FF in Phase III. Phase I: basal stage before gonadotropin administration (the early follicular phase); Phase II: approximately 8 days after gonadotropin administration (the late follicular phase); and Phase III: on the ovum pick-up day (the ovulatory phase). Values are reported as mean ± SD, *** p < 0.001.

Article Snippet: After the fourth passage, the KGN cells were incubated with serum-free DMEM/F-12 medium as the control; 10 −8 M FSH (Abcam, Cambridge, UK); 10 −8 M insulin-like growth factor-1 (IGF-1) (Sigma-Aldrich, St. Louis, MO, USA); 10 −8 M FSH together with 10 −8 M IGF-1 (FSH+IGF-1); 300 nM of globular adiponectin (Adipo) (Merck KGaA, Darmstadt, Germany); and 300 nM of adiponectin together with 10 −8 M FSH and 10 −8 M IGF-1 (Adipo+FSH+IGF-1).

Techniques: In Vitro, Injection

Journal: Life

Article Title: A Longitudinal Study of the Relationship of Adiponectin with Reproduction in Infertile Women Undergoing IVF/ICSI Treatment, and an Experimental Study in Human Granulosa Cells

doi: 10.3390/life13040994

Figure Lengend Snippet: The correlations between serum adiponectin, FF adiponectin, and hormonal levels in the various phases of the IVF/ICSI treatment.

Article Snippet: After the fourth passage, the KGN cells were incubated with serum-free DMEM/F-12 medium as the control; 10 −8 M FSH (Abcam, Cambridge, UK); 10 −8 M insulin-like growth factor-1 (IGF-1) (Sigma-Aldrich, St. Louis, MO, USA); 10 −8 M FSH together with 10 −8 M IGF-1 (FSH+IGF-1); 300 nM of globular adiponectin (Adipo) (Merck KGaA, Darmstadt, Germany); and 300 nM of adiponectin together with 10 −8 M FSH and 10 −8 M IGF-1 (Adipo+FSH+IGF-1).

Techniques:

Journal: Life

Article Title: A Longitudinal Study of the Relationship of Adiponectin with Reproduction in Infertile Women Undergoing IVF/ICSI Treatment, and an Experimental Study in Human Granulosa Cells

doi: 10.3390/life13040994

Figure Lengend Snippet: Summary of results for adiponectin in the in vitro fertilization (IVF)/intracytoplasmic sperm injection (ICSI) treatment. S: successful; U: unsuccessful; Se: serum; FF: follicular fluid; Adipo: adiponectin; FSH: follicle-stimulating hormone; LH: luteinizing hormone; Phase I: basal stage before gonadotropin administration (the early follicular phase); Phase II: approximately 8 days after gonadotropin administration (the late follicular phase); and Phase III: on the ovum pick-up day (the ovulatory phase); : increased, : comparable, : positive correlation, : negative correlation.

Article Snippet: After the fourth passage, the KGN cells were incubated with serum-free DMEM/F-12 medium as the control; 10 −8 M FSH (Abcam, Cambridge, UK); 10 −8 M insulin-like growth factor-1 (IGF-1) (Sigma-Aldrich, St. Louis, MO, USA); 10 −8 M FSH together with 10 −8 M IGF-1 (FSH+IGF-1); 300 nM of globular adiponectin (Adipo) (Merck KGaA, Darmstadt, Germany); and 300 nM of adiponectin together with 10 −8 M FSH and 10 −8 M IGF-1 (Adipo+FSH+IGF-1).

Techniques: In Vitro, Injection

Journal: Life

Article Title: A Longitudinal Study of the Relationship of Adiponectin with Reproduction in Infertile Women Undergoing IVF/ICSI Treatment, and an Experimental Study in Human Granulosa Cells

doi: 10.3390/life13040994

Figure Lengend Snippet: Effects of adiponectin on the CYP19A1 (aromatase) and follicle-stimulating hormone receptor ( FSHR ) mRNA expression in KGN cells ( n = 3). ( a ) CYP19A1 mRNA expression; ( b ) FSHR mRNA expression. Ctrl: control; FSH: 10 −8 M follicle-stimulating hormone; IGF-1: 10 −8 M insulin-like growth factor-1; Adipo: 300 nM adiponectin. Values are presented as mean ± SD.

Article Snippet: After the fourth passage, the KGN cells were incubated with serum-free DMEM/F-12 medium as the control; 10 −8 M FSH (Abcam, Cambridge, UK); 10 −8 M insulin-like growth factor-1 (IGF-1) (Sigma-Aldrich, St. Louis, MO, USA); 10 −8 M FSH together with 10 −8 M IGF-1 (FSH+IGF-1); 300 nM of globular adiponectin (Adipo) (Merck KGaA, Darmstadt, Germany); and 300 nM of adiponectin together with 10 −8 M FSH and 10 −8 M IGF-1 (Adipo+FSH+IGF-1).

Techniques: Expressing, Control