Journal: PLoS ONE

Article Title: Primed to vocalize: Wild-derived male house mice increase vocalization rate and diversity after a previous encounter with a female

doi: 10.1371/journal.pone.0242959

Figure Lengend Snippet: Classification of USVs: USV types, their abbreviations, a spectrogram’s example and definitions following the classification of [ , – ].

Article Snippet: Spectro-temporal features of USVs: amplitude (GLMM) , , , , , , , Relative Likelihood.

Techniques:

Journal: PLoS ONE

Article Title: Primed to vocalize: Wild-derived male house mice increase vocalization rate and diversity after a previous encounter with a female

doi: 10.1371/journal.pone.0242959

Figure Lengend Snippet: Approximately half of the males emitted less than 50 USVs during the 10 min trials, though some mice were very vocal (n = 50).

Article Snippet: Spectro-temporal features of USVs: amplitude (GLMM) , , , , , , , Relative Likelihood.

Techniques:

Journal: PLoS ONE

Article Title: Primed to vocalize: Wild-derived male house mice increase vocalization rate and diversity after a previous encounter with a female

doi: 10.1371/journal.pone.0242959

Figure Lengend Snippet: Boxplots of the number of USVs emitted by unprimed (control) and primed males. Boxes around the median (horizontal line) show the interquartile range (quartile 1 to 3) and whiskers extend to 1.5 times this range, or to the most extreme point, whichever is closer to the median. Extreme points are shown as circles. Different letters denote significant differences (p<0.05).

Article Snippet: Spectro-temporal features of USVs: amplitude (GLMM) , , , , , , , Relative Likelihood.

Techniques: Control

Journal: PLoS ONE

Article Title: Primed to vocalize: Wild-derived male house mice increase vocalization rate and diversity after a previous encounter with a female

doi: 10.1371/journal.pone.0242959

Figure Lengend Snippet: Tables 1 to 5 are regression tables of effects of priming on various aspects of vocalization in male mice.

Article Snippet: Spectro-temporal features of USVs: amplitude (GLMM) , , , , , , , Relative Likelihood.

Techniques:

Journal: PLoS ONE

Article Title: Primed to vocalize: Wild-derived male house mice increase vocalization rate and diversity after a previous encounter with a female

doi: 10.1371/journal.pone.0242959

Figure Lengend Snippet: The two spectrograms show a 10 s continuous sequence of the males that emitted most USVs in both groups, (A) the unprimed group and (B) in the group recorded 1d after priming. All lines of the spectrograms are continuous and each line shows 2 s (50 ms interval) of the 10 s sequence. Y-axes represent frequencies between 0–150 kHz with intervals of 25 kHz. Letters indicate examples of vocalization types, following the definitions and abbreviations in . LFV = low-frequency vocalization.

Article Snippet: Spectro-temporal features of USVs: amplitude (GLMM) , , , , , , , Relative Likelihood.

Techniques: Sequencing

Journal: PLoS ONE

Article Title: Primed to vocalize: Wild-derived male house mice increase vocalization rate and diversity after a previous encounter with a female

doi: 10.1371/journal.pone.0242959

Figure Lengend Snippet: Regression table of effects of priming on the spectro-temporal features of USVs in male mice.

Article Snippet: Spectro-temporal features of USVs: amplitude (GLMM) , , , , , , , Relative Likelihood.

Techniques:

Journal: Cell reports

Article Title: Dysregulation of Streptococcus pneumoniae zinc homeostasis breaks ampicillin resistance in a pneumonia infection model

doi: 10.1016/j.celrep.2021.110202

Figure Lengend Snippet: (A) RNA sequencing of S. pneumoniae D39 to determine relative gene expression of Zn-treated cultures compared to untreated, expressed as log 2 -fold change. Each dot represents a gene, distributed on the x axis in accordance with locus tag numbering for D39. Genes more highly expressed in the presence of 100 μM ZnSO 4 are present above the x axis (blue), with those below the x axis expressed at a lower level (yellow). Genes of interest are annotated with their putative or characterized functions. (B) Heatmap of changes in relative abundance of S. pneumoniae D39 key metabolites upon exposure to 100 μM ZnSO 4 . Each row represents a metabolite and each column represents an independent biological sample (n = 4). The relative abundance value of each sample metabolite is plotted in blue-pink color scale, where the pink color of the tile represents increased abundance and blue indicates decreased abundance. (C) Biosynthetic pathway involving GlmM and GlmU with the relative abundance of key metabolites shown (color scheme as in B) and the impact of Zn. (D and E) Spectrophotometric analysis of GlmU acetyltransferase activity (D) or uridyltransferase activity (E) in the presence of increasing Zn concentrations. Data are fit by nonlinear regression using the Michaelis-Menten (D) or uncompetitive inhibition (E) kinetic models with individual data points representing the mean (± standard error of the mean) of triplicate analyses. (F) GlmU structure (PDB ID: 7KR9) determined to 1.90 Å resolution shown in cartoon representation in complex with acetyl-CoA (light pink spheres), calcium (white spheres) and Zn (gray sphere). The uridyltransferase domain is represented in blue and the acetyltransferase domain in red, with the linking helix and C-terminal extension in gray. (G) Global amino acid variation of S. pneumoniae GlmU mapped against the Zn-bound crystal structure (F). Frequency of residue conservation from 20,010 S. pneumoniae GlmU sequences represented as a color gradient. Thresholds were chosen to represent differing orders of magnitude for conservation, with thresholds set at 94%, 98%, 99%, and 100% sequence conservation at the residue. The locations of Glu315 and His330 are indicated by arrows. (H and I) Spectrophotometric analysis of GlmU E315A/H330A acetyltransferase activity (H) or uridyltransferase activity (I) in the presence of increasing Zn concentrations. Data are fit by nonlinear regression using the Michaelis-Menten enzyme kinetic model with individual data points representing the mean (± standard error of the mean) of triplicate analyses.

Article Snippet: DNA sequences encoding GlmM (UniProtK – Q04J18) and GlmU (UniProt – Q04KU2) ordered from GenScript (Hong Kong) Ltd. in plasmid pUC57, were amplified via PCR and subcloned into pGEX-6P-1.

Techniques: RNA Sequencing, Gene Expression, Activity Assay, Inhibition, Residue, Sequencing

Journal: Cell reports

Article Title: Dysregulation of Streptococcus pneumoniae zinc homeostasis breaks ampicillin resistance in a pneumonia infection model

doi: 10.1016/j.celrep.2021.110202

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: DNA sequences encoding GlmM (UniProtK – Q04J18) and GlmU (UniProt – Q04KU2) ordered from GenScript (Hong Kong) Ltd. in plasmid pUC57, were amplified via PCR and subcloned into pGEX-6P-1.

Techniques: Virus, Recombinant, SYBR Green Assay, RNA Sequencing, Expressing, Construct, Plasmid Preparation, Software, Microscopy, Targeted Proteomics