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  • 94
    Alomone Labs girk2
    Girk2, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Thermo Fisher gene exp kcnj6 mm01215647 m1
    Gene Exp Kcnj6 Mm01215647 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher gene exp kcnj6 mm00440070 m1
    Gene Exp Kcnj6 Mm00440070 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Alomone Labs girk2 antibodies
    Girk2 Antibodies, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Thermo Fisher gene exp kcnj6 hs00158423 m1
    General characterization of neurons derived from PARK2 KO and healthy isogenic induced pluripotent stem cells (iPSCs). ( A ) Graphical overview of the differentiation of iPSCs to neural stem cells (NSCs) and fully committed dopaminergic neurons. ( B ) Immunofluorescence analysis of MAP2 (mature neurons, red) and TH (dopaminergic neurons, green) expression in PARK2 KO iPSC and control iPSC lines at day 25 of differentiation. Cell nuclei are visualized using DAPI (blue). Representative pictures are shown. Scale bar: 50 µm. ( C , D ) Quantitative assessment of ( C ) MAP2 + mature neurons and (D) TH + dopaminergic neurons in PARK2 KO and control iPSC lines. Data are presented as mean ± SEM, n = 15, 5 independent differentiations. ( E , F ) Western blotting and densitometry analysis of ( E ) MAP2 and ( F ) TH protein expression levels in PARK2 KO and control iPSC lines. Protein expression levels were normalized to α-actin. Full-length blots are presented in Supplementary Fig. . Data are presented as mean ± SEM, n = 3, 3 independent experiments. ( G ) qRT-PCR analysis for midbrain/dopaminergic markers (EN1, NURR1, and <t>GIRK2).</t> GAPDH, 18S, HPRT were used as endogenous references. Data were normalized to control levels and presented as mean ± SEM, n = 4, 2 independent experiments.
    Gene Exp Kcnj6 Hs00158423 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    General characterization of neurons derived from PARK2 KO and healthy isogenic induced pluripotent stem cells (iPSCs). ( A ) Graphical overview of the differentiation of iPSCs to neural stem cells (NSCs) and fully committed dopaminergic neurons. ( B ) Immunofluorescence analysis of MAP2 (mature neurons, red) and TH (dopaminergic neurons, green) expression in PARK2 KO iPSC and control iPSC lines at day 25 of differentiation. Cell nuclei are visualized using DAPI (blue). Representative pictures are shown. Scale bar: 50 µm. ( C , D ) Quantitative assessment of ( C ) MAP2 + mature neurons and (D) TH + dopaminergic neurons in PARK2 KO and control iPSC lines. Data are presented as mean ± SEM, n = 15, 5 independent differentiations. ( E , F ) Western blotting and densitometry analysis of ( E ) MAP2 and ( F ) TH protein expression levels in PARK2 KO and control iPSC lines. Protein expression levels were normalized to α-actin. Full-length blots are presented in Supplementary Fig. . Data are presented as mean ± SEM, n = 3, 3 independent experiments. ( G ) qRT-PCR analysis for midbrain/dopaminergic markers (EN1, NURR1, and GIRK2). GAPDH, 18S, HPRT were used as endogenous references. Data were normalized to control levels and presented as mean ± SEM, n = 4, 2 independent experiments.

    Journal: Scientific Reports

    Article Title: Lysosomal perturbations in human dopaminergic neurons derived from induced pluripotent stem cells with PARK2 mutation

    doi: 10.1038/s41598-020-67091-6

    Figure Lengend Snippet: General characterization of neurons derived from PARK2 KO and healthy isogenic induced pluripotent stem cells (iPSCs). ( A ) Graphical overview of the differentiation of iPSCs to neural stem cells (NSCs) and fully committed dopaminergic neurons. ( B ) Immunofluorescence analysis of MAP2 (mature neurons, red) and TH (dopaminergic neurons, green) expression in PARK2 KO iPSC and control iPSC lines at day 25 of differentiation. Cell nuclei are visualized using DAPI (blue). Representative pictures are shown. Scale bar: 50 µm. ( C , D ) Quantitative assessment of ( C ) MAP2 + mature neurons and (D) TH + dopaminergic neurons in PARK2 KO and control iPSC lines. Data are presented as mean ± SEM, n = 15, 5 independent differentiations. ( E , F ) Western blotting and densitometry analysis of ( E ) MAP2 and ( F ) TH protein expression levels in PARK2 KO and control iPSC lines. Protein expression levels were normalized to α-actin. Full-length blots are presented in Supplementary Fig. . Data are presented as mean ± SEM, n = 3, 3 independent experiments. ( G ) qRT-PCR analysis for midbrain/dopaminergic markers (EN1, NURR1, and GIRK2). GAPDH, 18S, HPRT were used as endogenous references. Data were normalized to control levels and presented as mean ± SEM, n = 4, 2 independent experiments.

    Article Snippet: Relative gene expression was assessed using the following TagMan assays: GAPDH , Hs02758991_g1; 18 S , Hs03003631_g1; HPRT , Hs02800695_m1; EN1 , Hs00154977_m1; NURR1 , Hs00428691_m1; GIRK2 , Hs00158423_m1.

    Techniques: Derivative Assay, Immunofluorescence, Expressing, Western Blot, Quantitative RT-PCR