gexscope single-cell matrix data Search Results


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Singleron Biotechnologies gexscope single cell rna seq library kit
Gexscope Single Cell Rna Seq Library Kit, supplied by Singleron Biotechnologies, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Singleron Biotechnologies gexscope single cell rna seq kit
Gexscope Single Cell Rna Seq Kit, supplied by Singleron Biotechnologies, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Singleron Biotechnologies scrna seq libraries
<t>scRNA‐seq</t> <t>analysis</t> of BM neutrophils of 3‐mon male SAMP6 and SAMR1. (A) Flowchart of scRNA‐seq analysis of BM cells of 3‐mon male SAMP6 and SAMR1. (B) UMAP profile indicated cell clusters of BM cells of SAMP6 and SAMR1. (C) UMAP profile indicated SAMP6 and SAMR1 BM neutrophils contained 9 subsets. Pseudo‐time analysis (green curve) of neutrophils subsets. (D) Analysis of neutrophil differentiation–related genes in 9 subsets, and C12 was identified as the least mature and C17 as the most mature, consistent with differentiation trajectory of pseudo‐time analysis. (E) Proportion analysis of neutrophil subsets in SAMP6 and SAMR1 indicated C10 accumulation in SAMP6. (F) Violin plot showed the CXCR4 and CXCR2 expression in neutrophil subsets along with neutrophil maturation. High expression of CXCR4 and intermediate expression of CXCR2 was shown in C10. (G) Heatmap of gene expression showed CD55 significantly upregulated in C10. (H) Violin plot showed the highest expression of CD55 in C10. (I) Projection of CD55‐expression in SAMP6 BM neutrophils showed its expression concentrated in C10. (J) Pseudo‐time analysis of CD55 expression trajectory indicated it exclusively expressed in the middle of neutrophil differentiation. (K) KEGG analysis indicated NETs‐formation, ROS‐related pathway and HIF1‐related pathway significantly activated in neutrophil subset C10.
Scrna Seq Libraries, supplied by Singleron Biotechnologies, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Singleron Biotechnologies gexscope microfluidics chips
<t>scRNA‐seq</t> <t>analysis</t> of BM neutrophils of 3‐mon male SAMP6 and SAMR1. (A) Flowchart of scRNA‐seq analysis of BM cells of 3‐mon male SAMP6 and SAMR1. (B) UMAP profile indicated cell clusters of BM cells of SAMP6 and SAMR1. (C) UMAP profile indicated SAMP6 and SAMR1 BM neutrophils contained 9 subsets. Pseudo‐time analysis (green curve) of neutrophils subsets. (D) Analysis of neutrophil differentiation–related genes in 9 subsets, and C12 was identified as the least mature and C17 as the most mature, consistent with differentiation trajectory of pseudo‐time analysis. (E) Proportion analysis of neutrophil subsets in SAMP6 and SAMR1 indicated C10 accumulation in SAMP6. (F) Violin plot showed the CXCR4 and CXCR2 expression in neutrophil subsets along with neutrophil maturation. High expression of CXCR4 and intermediate expression of CXCR2 was shown in C10. (G) Heatmap of gene expression showed CD55 significantly upregulated in C10. (H) Violin plot showed the highest expression of CD55 in C10. (I) Projection of CD55‐expression in SAMP6 BM neutrophils showed its expression concentrated in C10. (J) Pseudo‐time analysis of CD55 expression trajectory indicated it exclusively expressed in the middle of neutrophil differentiation. (K) KEGG analysis indicated NETs‐formation, ROS‐related pathway and HIF1‐related pathway significantly activated in neutrophil subset C10.
Gexscope Microfluidics Chips, supplied by Singleron Biotechnologies, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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<t>scRNA‐seq</t> <t>analysis</t> of BM neutrophils of 3‐mon male SAMP6 and SAMR1. (A) Flowchart of scRNA‐seq analysis of BM cells of 3‐mon male SAMP6 and SAMR1. (B) UMAP profile indicated cell clusters of BM cells of SAMP6 and SAMR1. (C) UMAP profile indicated SAMP6 and SAMR1 BM neutrophils contained 9 subsets. Pseudo‐time analysis (green curve) of neutrophils subsets. (D) Analysis of neutrophil differentiation–related genes in 9 subsets, and C12 was identified as the least mature and C17 as the most mature, consistent with differentiation trajectory of pseudo‐time analysis. (E) Proportion analysis of neutrophil subsets in SAMP6 and SAMR1 indicated C10 accumulation in SAMP6. (F) Violin plot showed the CXCR4 and CXCR2 expression in neutrophil subsets along with neutrophil maturation. High expression of CXCR4 and intermediate expression of CXCR2 was shown in C10. (G) Heatmap of gene expression showed CD55 significantly upregulated in C10. (H) Violin plot showed the highest expression of CD55 in C10. (I) Projection of CD55‐expression in SAMP6 BM neutrophils showed its expression concentrated in C10. (J) Pseudo‐time analysis of CD55 expression trajectory indicated it exclusively expressed in the middle of neutrophil differentiation. (K) KEGG analysis indicated NETs‐formation, ROS‐related pathway and HIF1‐related pathway significantly activated in neutrophil subset C10.
Chromium Next Gem Single Cell 3ʹ Reagent Kits V3 1, supplied by 10X Genomics, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Singleron Biotechnologies microfluidic devices
<t>scRNA‐seq</t> <t>analysis</t> of BM neutrophils of 3‐mon male SAMP6 and SAMR1. (A) Flowchart of scRNA‐seq analysis of BM cells of 3‐mon male SAMP6 and SAMR1. (B) UMAP profile indicated cell clusters of BM cells of SAMP6 and SAMR1. (C) UMAP profile indicated SAMP6 and SAMR1 BM neutrophils contained 9 subsets. Pseudo‐time analysis (green curve) of neutrophils subsets. (D) Analysis of neutrophil differentiation–related genes in 9 subsets, and C12 was identified as the least mature and C17 as the most mature, consistent with differentiation trajectory of pseudo‐time analysis. (E) Proportion analysis of neutrophil subsets in SAMP6 and SAMR1 indicated C10 accumulation in SAMP6. (F) Violin plot showed the CXCR4 and CXCR2 expression in neutrophil subsets along with neutrophil maturation. High expression of CXCR4 and intermediate expression of CXCR2 was shown in C10. (G) Heatmap of gene expression showed CD55 significantly upregulated in C10. (H) Violin plot showed the highest expression of CD55 in C10. (I) Projection of CD55‐expression in SAMP6 BM neutrophils showed its expression concentrated in C10. (J) Pseudo‐time analysis of CD55 expression trajectory indicated it exclusively expressed in the middle of neutrophil differentiation. (K) KEGG analysis indicated NETs‐formation, ROS‐related pathway and HIF1‐related pathway significantly activated in neutrophil subset C10.
Microfluidic Devices, supplied by Singleron Biotechnologies, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Singleron Biotechnologies singleron matrix
<t>scRNA‐seq</t> <t>analysis</t> of BM neutrophils of 3‐mon male SAMP6 and SAMR1. (A) Flowchart of scRNA‐seq analysis of BM cells of 3‐mon male SAMP6 and SAMR1. (B) UMAP profile indicated cell clusters of BM cells of SAMP6 and SAMR1. (C) UMAP profile indicated SAMP6 and SAMR1 BM neutrophils contained 9 subsets. Pseudo‐time analysis (green curve) of neutrophils subsets. (D) Analysis of neutrophil differentiation–related genes in 9 subsets, and C12 was identified as the least mature and C17 as the most mature, consistent with differentiation trajectory of pseudo‐time analysis. (E) Proportion analysis of neutrophil subsets in SAMP6 and SAMR1 indicated C10 accumulation in SAMP6. (F) Violin plot showed the CXCR4 and CXCR2 expression in neutrophil subsets along with neutrophil maturation. High expression of CXCR4 and intermediate expression of CXCR2 was shown in C10. (G) Heatmap of gene expression showed CD55 significantly upregulated in C10. (H) Violin plot showed the highest expression of CD55 in C10. (I) Projection of CD55‐expression in SAMP6 BM neutrophils showed its expression concentrated in C10. (J) Pseudo‐time analysis of CD55 expression trajectory indicated it exclusively expressed in the middle of neutrophil differentiation. (K) KEGG analysis indicated NETs‐formation, ROS‐related pathway and HIF1‐related pathway significantly activated in neutrophil subset C10.
Singleron Matrix, supplied by Singleron Biotechnologies, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Becton Dickinson rhapsody wta reagent kit
<t>scRNA‐seq</t> <t>analysis</t> of BM neutrophils of 3‐mon male SAMP6 and SAMR1. (A) Flowchart of scRNA‐seq analysis of BM cells of 3‐mon male SAMP6 and SAMR1. (B) UMAP profile indicated cell clusters of BM cells of SAMP6 and SAMR1. (C) UMAP profile indicated SAMP6 and SAMR1 BM neutrophils contained 9 subsets. Pseudo‐time analysis (green curve) of neutrophils subsets. (D) Analysis of neutrophil differentiation–related genes in 9 subsets, and C12 was identified as the least mature and C17 as the most mature, consistent with differentiation trajectory of pseudo‐time analysis. (E) Proportion analysis of neutrophil subsets in SAMP6 and SAMR1 indicated C10 accumulation in SAMP6. (F) Violin plot showed the CXCR4 and CXCR2 expression in neutrophil subsets along with neutrophil maturation. High expression of CXCR4 and intermediate expression of CXCR2 was shown in C10. (G) Heatmap of gene expression showed CD55 significantly upregulated in C10. (H) Violin plot showed the highest expression of CD55 in C10. (I) Projection of CD55‐expression in SAMP6 BM neutrophils showed its expression concentrated in C10. (J) Pseudo‐time analysis of CD55 expression trajectory indicated it exclusively expressed in the middle of neutrophil differentiation. (K) KEGG analysis indicated NETs‐formation, ROS‐related pathway and HIF1‐related pathway significantly activated in neutrophil subset C10.
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Illumina Inc illumina novaseq 6000 platform
<t>scRNA‐seq</t> <t>analysis</t> of BM neutrophils of 3‐mon male SAMP6 and SAMR1. (A) Flowchart of scRNA‐seq analysis of BM cells of 3‐mon male SAMP6 and SAMR1. (B) UMAP profile indicated cell clusters of BM cells of SAMP6 and SAMR1. (C) UMAP profile indicated SAMP6 and SAMR1 BM neutrophils contained 9 subsets. Pseudo‐time analysis (green curve) of neutrophils subsets. (D) Analysis of neutrophil differentiation–related genes in 9 subsets, and C12 was identified as the least mature and C17 as the most mature, consistent with differentiation trajectory of pseudo‐time analysis. (E) Proportion analysis of neutrophil subsets in SAMP6 and SAMR1 indicated C10 accumulation in SAMP6. (F) Violin plot showed the CXCR4 and CXCR2 expression in neutrophil subsets along with neutrophil maturation. High expression of CXCR4 and intermediate expression of CXCR2 was shown in C10. (G) Heatmap of gene expression showed CD55 significantly upregulated in C10. (H) Violin plot showed the highest expression of CD55 in C10. (I) Projection of CD55‐expression in SAMP6 BM neutrophils showed its expression concentrated in C10. (J) Pseudo‐time analysis of CD55 expression trajectory indicated it exclusively expressed in the middle of neutrophil differentiation. (K) KEGG analysis indicated NETs‐formation, ROS‐related pathway and HIF1‐related pathway significantly activated in neutrophil subset C10.
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Image Search Results


scRNA‐seq analysis of BM neutrophils of 3‐mon male SAMP6 and SAMR1. (A) Flowchart of scRNA‐seq analysis of BM cells of 3‐mon male SAMP6 and SAMR1. (B) UMAP profile indicated cell clusters of BM cells of SAMP6 and SAMR1. (C) UMAP profile indicated SAMP6 and SAMR1 BM neutrophils contained 9 subsets. Pseudo‐time analysis (green curve) of neutrophils subsets. (D) Analysis of neutrophil differentiation–related genes in 9 subsets, and C12 was identified as the least mature and C17 as the most mature, consistent with differentiation trajectory of pseudo‐time analysis. (E) Proportion analysis of neutrophil subsets in SAMP6 and SAMR1 indicated C10 accumulation in SAMP6. (F) Violin plot showed the CXCR4 and CXCR2 expression in neutrophil subsets along with neutrophil maturation. High expression of CXCR4 and intermediate expression of CXCR2 was shown in C10. (G) Heatmap of gene expression showed CD55 significantly upregulated in C10. (H) Violin plot showed the highest expression of CD55 in C10. (I) Projection of CD55‐expression in SAMP6 BM neutrophils showed its expression concentrated in C10. (J) Pseudo‐time analysis of CD55 expression trajectory indicated it exclusively expressed in the middle of neutrophil differentiation. (K) KEGG analysis indicated NETs‐formation, ROS‐related pathway and HIF1‐related pathway significantly activated in neutrophil subset C10.

Journal: Advanced Science

Article Title: ROS Activated NETosis of Bone Marrow CD55 + Intermediate Mature Neutrophils Through HIF1α‐PADI4 Pathway to Initiate Bone Aging

doi: 10.1002/advs.202500046

Figure Lengend Snippet: scRNA‐seq analysis of BM neutrophils of 3‐mon male SAMP6 and SAMR1. (A) Flowchart of scRNA‐seq analysis of BM cells of 3‐mon male SAMP6 and SAMR1. (B) UMAP profile indicated cell clusters of BM cells of SAMP6 and SAMR1. (C) UMAP profile indicated SAMP6 and SAMR1 BM neutrophils contained 9 subsets. Pseudo‐time analysis (green curve) of neutrophils subsets. (D) Analysis of neutrophil differentiation–related genes in 9 subsets, and C12 was identified as the least mature and C17 as the most mature, consistent with differentiation trajectory of pseudo‐time analysis. (E) Proportion analysis of neutrophil subsets in SAMP6 and SAMR1 indicated C10 accumulation in SAMP6. (F) Violin plot showed the CXCR4 and CXCR2 expression in neutrophil subsets along with neutrophil maturation. High expression of CXCR4 and intermediate expression of CXCR2 was shown in C10. (G) Heatmap of gene expression showed CD55 significantly upregulated in C10. (H) Violin plot showed the highest expression of CD55 in C10. (I) Projection of CD55‐expression in SAMP6 BM neutrophils showed its expression concentrated in C10. (J) Pseudo‐time analysis of CD55 expression trajectory indicated it exclusively expressed in the middle of neutrophil differentiation. (K) KEGG analysis indicated NETs‐formation, ROS‐related pathway and HIF1‐related pathway significantly activated in neutrophil subset C10.

Article Snippet: Single‐cell suspensions were then loaded onto microfluidic devices and scRNA‐seq libraries were constructed according to Singleron GEXSCOPE protocol by GEXSCOPE Single‐Cell RNA Library Kit (Singleron Biotechnologies) [ ].

Techniques: Expressing, Gene Expression