Journal: Frontiers in Medicine

Article Title: The MUC5B Promoter Polymorphism Associates With Severe COVID-19 in the European Population

doi: 10.3389/fmed.2021.668024

Figure Lengend Snippet: MUC5B rs35705950 genotype in white subjects with severe COVID-19 and controls.

Article Snippet: The discovery cohort was genotyped for MUC5B rs35705950 genotype with a pre-designed taqman SNP genotyping assay and the QuantStudio® 5 Real-Time PCR system (both ThermoFisher Scientific, Waltham, Massachusetts, USA).

Techniques: Biomarker Discovery

Journal: Frontiers in Medicine

Article Title: The MUC5B Promoter Polymorphism Associates With Severe COVID-19 in the European Population

doi: 10.3389/fmed.2021.668024

Figure Lengend Snippet: Multivariate association analysis for COVID-19 in the UK biobank validation cohort.

Article Snippet: The discovery cohort was genotyped for MUC5B rs35705950 genotype with a pre-designed taqman SNP genotyping assay and the QuantStudio® 5 Real-Time PCR system (both ThermoFisher Scientific, Waltham, Massachusetts, USA).

Techniques: Biomarker Discovery

Journal: Frontiers in Medicine

Article Title: The MUC5B Promoter Polymorphism Associates With Severe COVID-19 in the European Population

doi: 10.3389/fmed.2021.668024

Figure Lengend Snippet: Forest plots of meta-analyses of the association of MUC5B rs35705950 with severe COVID-19 in subjects of European ancestry. Dotted line represents the odds ratio from the combined analysis. (A) Meta-analysis of allele contrast (T vs. G; P combined analysis = 6.63 e-06). (B) Meta-analysis of the dominant T-allele carriage model (GT+TT vs. GG; P combined analysis = 2.05e-05). CI, confidence interval.

Article Snippet: The discovery cohort was genotyped for MUC5B rs35705950 genotype with a pre-designed taqman SNP genotyping assay and the QuantStudio® 5 Real-Time PCR system (both ThermoFisher Scientific, Waltham, Massachusetts, USA).

Techniques:

Journal: European Journal of Clinical Microbiology & Infectious Diseases

Article Title: Impact of IL-28B polymorphisms on pegylated interferon plus ribavirin treatment response in children and adolescents infected with HCV genotypes 1 and 4

doi: 10.1007/s10096-012-1799-z

Figure Lengend Snippet: Characteristics of patients treated with pegylated interferon (peg-IFN) and ribavirin (RBV)

Article Snippet: In order to perform the RFLP assay for the rs12979860 genotype, 15 μL of amplicons were digested with 2 U of BstUI restriction endonuclease (Fermentas) at 37 °C for 12 h. BstUI digestion of allele CC yields fragments of 136, 82, and 25 base pairs, whereas DNA containing the allele TT polymorphism yields fragments of 161 and 82 base pairs.

Techniques: Activity Assay

Journal: European Journal of Clinical Microbiology & Infectious Diseases

Article Title: Impact of IL-28B polymorphisms on pegylated interferon plus ribavirin treatment response in children and adolescents infected with HCV genotypes 1 and 4

doi: 10.1007/s10096-012-1799-z

Figure Lengend Snippet: Role of interleukin 28B (IL-28B) gene polymorphisms on virological response in children and adolescents with hepatitis C virus (HCV) genotypes 1 and 4

Article Snippet: In order to perform the RFLP assay for the rs12979860 genotype, 15 μL of amplicons were digested with 2 U of BstUI restriction endonuclease (Fermentas) at 37 °C for 12 h. BstUI digestion of allele CC yields fragments of 136, 82, and 25 base pairs, whereas DNA containing the allele TT polymorphism yields fragments of 161 and 82 base pairs.

Techniques: Virus

Journal: European Journal of Clinical Microbiology & Infectious Diseases

Article Title: Impact of IL-28B polymorphisms on pegylated interferon plus ribavirin treatment response in children and adolescents infected with HCV genotypes 1 and 4

doi: 10.1007/s10096-012-1799-z

Figure Lengend Snippet: The association of the interleukin 28B (IL-28B) single-nucleotide polymorphism (SNP) with hepatitis C virus (HCV) baseline viral load in chronic HCV patients. There were significant differences in viral load between patients with rs12979860 genotype CC (median, 7.1 × 10 5 IU/ml; range, 6.3 × 10 4 –5.4 × 10 6 IU/ml) and CT–TT (2.9 × 10 5 IU/ml; range, 3.7 × 10 3 –4.7 × 10 6 IU/ml; p = 0.010). Statistical analysis was performed using a Mann–Whitney U -test

Article Snippet: In order to perform the RFLP assay for the rs12979860 genotype, 15 μL of amplicons were digested with 2 U of BstUI restriction endonuclease (Fermentas) at 37 °C for 12 h. BstUI digestion of allele CC yields fragments of 136, 82, and 25 base pairs, whereas DNA containing the allele TT polymorphism yields fragments of 161 and 82 base pairs.

Techniques: Virus, MANN-WHITNEY

Journal: European Journal of Clinical Microbiology & Infectious Diseases

Article Title: Impact of IL-28B polymorphisms on pegylated interferon plus ribavirin treatment response in children and adolescents infected with HCV genotypes 1 and 4

doi: 10.1007/s10096-012-1799-z

Figure Lengend Snippet: Impact of IL-28B SNP on high baseline viral load according to 600,000 and 800,000 IU/ml stratification

Article Snippet: In order to perform the RFLP assay for the rs12979860 genotype, 15 μL of amplicons were digested with 2 U of BstUI restriction endonuclease (Fermentas) at 37 °C for 12 h. BstUI digestion of allele CC yields fragments of 136, 82, and 25 base pairs, whereas DNA containing the allele TT polymorphism yields fragments of 161 and 82 base pairs.

Techniques:

Journal: Pharmacogenetics and genomics

Article Title: The functional G143E variant of carboxylesterase 1 is associated with increased clopidogrel active metabolite levels and greater clopidogrel response

doi: 10.1097/FPC.0b013e32835aa8a2

Figure Lengend Snippet: Schematic of the major enzymatic steps in clopidogrel metabolism. CES1, carboxylesterase 1; CYP, cytochrome P450.

Article Snippet: CES1 G143E (rs71647871) SNP genotyping was performed using a TaqMan SNP genotyping assay (Applied Biosystems, Foster City, California, USA).

Techniques:

Journal: Pharmacogenetics and genomics

Article Title: The functional G143E variant of carboxylesterase 1 is associated with increased clopidogrel active metabolite levels and greater clopidogrel response

doi: 10.1097/FPC.0b013e32835aa8a2

Figure Lengend Snippet: Association of the carboxylesterase 1 (CES1) G143E variant (rs71647871) with ADP-stimulated platelet aggregation before (a, b) and after (c, d) clopidogrel administration in participants of the Pharmacogenomics of Anti-Platelet Intervention (PAPI) study (a, c) and coronary intervention patients at Sinai Hospital of Baltimore (b, d).

Article Snippet: CES1 G143E (rs71647871) SNP genotyping was performed using a TaqMan SNP genotyping assay (Applied Biosystems, Foster City, California, USA).

Techniques: Variant Assay

Journal: Pharmacogenetics and genomics

Article Title: The functional G143E variant of carboxylesterase 1 is associated with increased clopidogrel active metabolite levels and greater clopidogrel response

doi: 10.1097/FPC.0b013e32835aa8a2

Figure Lengend Snippet: Effect of CES1 143E, CYP2C19 *2, and CYP2C19 *17 variants on postclopidogrel ADP-stimulated platelet aggregation in participants of the Amish Pharmacogenomics of Anti-Platelet Intervention study

Article Snippet: CES1 G143E (rs71647871) SNP genotyping was performed using a TaqMan SNP genotyping assay (Applied Biosystems, Foster City, California, USA).

Techniques: