Journal: Translational Oncology
Article Title: Non-invasive detection of biliary tract cancer by low-coverage whole genome sequencing from plasma cell-free DNA: A prospective cohort study
Figure Lengend Snippet: Overview of copy number variations via plasma cell-free DNA analysis in the included patients. (A) copy number variation genome of gallbladder cancer. (B) copy number variation genome of cholangiocarcinoma. (C) Copy number changes of benign biliary lesions. (D) heatmap of copy number variation quantified by chromosome Z-scores for all patients. GC, gallbladder cancer; CC, cholangiocarcinoma; BE, benign lesions.
Article Snippet: DNA was fragmented into an average size of 300 bp (cfDNA without fragmentation), and then 100 ng of fragmented genomic DNA (cfDNA 10 ng) was used for the preparation of sequencing libraries (NEBnext Ultra II).