Journal: PLOS Global Public Health

Article Title: Performance evaluation of SARS-CoV-2 rapid diagnostic tests in Nigeria: A cross-sectional study

doi: 10.1371/journal.pgph.0003371

Figure Lengend Snippet: List of PCR reagents supplied by NCDC.

Article Snippet: 2 , Gensig COVID-19 Real-Time PCR Assay , Primerdesign , ORF1ab.

Techniques: Real-time Polymerase Chain Reaction

Journal: Parasites & Vectors

Article Title: Refining the Schistosoma haematobium recombinase polymerase amplification (Sh-RPA) assay: moving towards point-of-care use in endemic settings

doi: 10.1186/s13071-024-06380-9

Figure Lengend Snippet: Comparison of Sh-RPA performance using DNA extracted from single S. haematobium eggs using different extraction methods. A Extracta DNA Prep (Quantabio) with 95 °C incubation. B Extracta DNA Prep (Quantabio) with room-temperature incubation. C DNeasy Blood & Tissue Kit (Qiagen) with 56 °C incubation. D DNeasy Blood & Tissue Kit (Qiagen) with room-temperature incubation. E SwiftX DNA Kit (Xpedite Diagnostics) with 95 °C incubation and modified protocol (see text). F SwiftX DNA Kit (Xpedite Diagnostics) with room-temperature incubation and modified protocol (see text). G Genesig Easy DNA/RNA Kit (PrimerDesign) with 95 °C incubation. H Direct egg addition to the Sh-RPA reaction with no extraction performed. I SpeedXtract Nucleic Acid Kit (Qiagen) with 95 °C incubation (single replicate extraction only, due to kit being discontinued). Dashed line represents threshold for a result to be considered positive (≥ 500 RFU)

Article Snippet: However, all Sh-RPA reactions failed for samples extracted using the Genesig Easy DNA/RNA Kit (Primerdesign) kit (Fig. G).

Techniques: Comparison, Extraction, Incubation, Modification