Journal: Nature Communications

Article Title: The gut microbiome is associated with susceptibility to febrile malaria in Malian children

doi: 10.1038/s41467-024-52953-8

Figure Lengend Snippet: A Principal coordinate analysis (PCoA) plot of the Bray-Curtis dissimilarity of the human fecal samples using 16S rRNA sequencing. Gavage samples are highlighted by increased point size. B Parasitemia and C AUC of the gavaged gnotobiotic mice by resistant and susceptible outcome groups, and D parasitemia by individual gavage groups. n = 4 mice for each donor used. n = 16 for the overall resistant and susceptible groups. PCoA plots of the Bray-Curtis dissimilarity for the E human and the F murine fecal samples using 16S rRNA sequencing and the updated resistant definition. The P value for the Bray–Curtis distance was determined using a two-sided PERMANOVA, and the P value for the parasitemia was determined using a two-sided Mann–Whitney U test. Blue circles indicate resistant children or low parasitemia mice, and red circles indicate susceptible children or high parasitemia mice. Mean ± standard error of the mean (SEM) indicated for parasitemia and AUC.

Article Snippet: Fecal samples were collected upon arrival and from the sentinels at the end of the experiment and sterility verified by IDEXX BioAnalytics through generic bacteria 16S rRNA gene PCR, and fungal, and aerobic and anaerobic bacteria culture (case numbers 120118-2022 and 125378-2022).

Techniques: Sequencing, MANN-WHITNEY

Journal: Arthritis Research & Therapy

Article Title: Automated interpretation of ANCA patterns - a new approach in the serology of ANCA-associated vasculitis

doi: 10.1186/ar4119

Figure Lengend Snippet: ANCA patterns and recognition thereof by mathematical algorithms . (a) Images (detail) of serum samples demonstrating C-ANCA, P-ANCA, and atypical ANCA patterns on ethN and formN taken automatically by AKLIDES™. Chromatin is stained by DAPI (blue) and specific ANCA interactions are revealed by FITC (green)-labeled secondary anti-human IgG. (b) Pattern recognition image of perinuclear (A) and cytoplasmic (B) specific staining of neutrophils used by the novel algorithms for pattern differentiation: DAPI (blue) and FITC fluorescence intensity signals (green) of respective images were combined and illustrated in three dimensions (x - object length, y - object width, light intensity of fluorescence signal). C-ANCA, cytoplasmic antineutrophil cytoplasmic antibody; ethN, ethanol-fixed neutrophils; formN, formalin-fixed neutrophils; P-ANCA, perinuclear antineutrophil cytoplasmic antibody.

Article Snippet: Employing IIF, ANCA were detected by running patient samples on ethN and formN according to the recommendations of the manufacturer (GA Generic Assays GmbH, Dahlewitz/Berlin, Germany).

Techniques: Staining, Labeling, Fluorescence

Journal: Arthritis Research & Therapy

Article Title: Automated interpretation of ANCA patterns - a new approach in the serology of ANCA-associated vasculitis

doi: 10.1186/ar4119

Figure Lengend Snippet: Comparison of manual and automated positive/negative discrimination assessing fluorescence intensity of 342 samples on ethN and formN patterns.

Article Snippet: Employing IIF, ANCA were detected by running patient samples on ethN and formN according to the recommendations of the manufacturer (GA Generic Assays GmbH, Dahlewitz/Berlin, Germany).

Techniques: Comparison, Fluorescence

Journal: Arthritis Research & Therapy

Article Title: Automated interpretation of ANCA patterns - a new approach in the serology of ANCA-associated vasculitis

doi: 10.1186/ar4119

Figure Lengend Snippet: Comparison of visual and automated interpretation of IIF ANCA patterns of the 'test set' patients ( n = 342) on ethN and formN.

Article Snippet: Employing IIF, ANCA were detected by running patient samples on ethN and formN according to the recommendations of the manufacturer (GA Generic Assays GmbH, Dahlewitz/Berlin, Germany).

Techniques: Comparison

Journal: Arthritis Research & Therapy

Article Title: Automated interpretation of ANCA patterns - a new approach in the serology of ANCA-associated vasculitis

doi: 10.1186/ar4119

Figure Lengend Snippet: Comparison of automated and visual interpretation of ANCA IIF patterns in patient and control samples of the 'test set' on ethN (A) and formN (B) . Patterns of ethN and formN were first detected automatically by AKLIDES™ and then interpreted manually by a routine fluorescence microscope. GPA, granulomatosis with polyangiitis (Wegener's) ( n = 59); EGPA, eosinophilic granulomatosis with polyangiitis (Churg-Strauss Syndrome) ( n = 40); MPA, microscopic polyangiitis ( n = 20); SLE, systemic lupus erythematosus ( n = 40); RA, rheumatoid arthritis ( n = 30); RA RV, rheumatoid arthritis with rheumatoid vasculitis ( n = 10); Cr V, cryoglobulinaemic vasculitis ( n = 10); HC, healthy control ( n = 44); S Scl, systemic sclerosis ( n = 38), Inf dis, infectious disease ( n = 51).

Article Snippet: Employing IIF, ANCA were detected by running patient samples on ethN and formN according to the recommendations of the manufacturer (GA Generic Assays GmbH, Dahlewitz/Berlin, Germany).

Techniques: Comparison, Control, Fluorescence, Microscopy