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Image Search Results
Journal: Nature Communications
Article Title: Octyl itaconate enhances VSVΔ51 oncolytic virotherapy by multitarget inhibition of antiviral and inflammatory pathways
doi: 10.1038/s41467-024-48422-x
Figure Lengend Snippet: a BALB/c mice were injected subcutaneously with 1 × 10 5 CT26WT tumor cells. Treatment via intratumoral (i.t.) injections with vehicle (40% CDX) in PBS or with 4-OI (25 mg/kg/dose) in 40% CDX in PBS for 24 h prior challenge with VSVΔ51 expressing firefly luciferase (10 8 PFU) was given as indicated by arrows. Mice were euthanized seven days after the first VSVΔ51 injection for analysis. b Mean ( ± SEM) tumor growth of the groups followed the start of the treatment regimen ( n = 3 in the CDX-PBS group; n = 5 in the CDX-4-OI/PBS group, n = 6 in CDX-VSV and CDX-4-OI/VSV groups). Statistics on tumor volumes at day 8 indicate significance by one-way ANOVA followed by Šídák’s multiple comparisons test on tumor sizes at day 8. c Relative expression analyzed by RT-qPCR of RNA isolated from bulk tumor samples. n = 3 in the CDX-PBS group; n = 5 in the CDX-4-OI/PBS group, n = 6 in CDX-VSV and n = 4 CDX-4-OI/VSV groups (two samples excluded due to insufficient tumor material) d Flow cytometry data indicating the distribution of main T-cell populations within tumors upon different treatments analyzed by manual gating in FlowJo software. Mean ± SEM is displayed and compared per treatment group. Each data point represents one animal. n = 3 in the CDX-PBS group; n = 5 in the CDX-4-OI/PBS group, n = 5 in CDX-VSV (one sample lost during acquisition), and n = 6 CDX-4-OI/VSV groups. e Expression intensity profile of myeloid markers on clustered live, CD45+, CD3− cells from merged samples ( n = 60, 3 different organs) to distinguish the regional expression of single myeloid markers. Relative expression is indicated by color where red indicates high expression and blue represents no expression within the cluster. f Opt-SNE cluster plots of live, CD45+, CD3− cells from indicated organs and treatment group ( n = 3 in the CDX-PBS group; n = 5 in the CDX-4-OI/PBS group, n = 6 in CDX-VSV and CDX-4-OI/VSV groups). Cell density is indicated by color where red indicates high density and blue indicates low density within the cluster. Analysis generated using OMIQ software. ( a ) was created using BioRender.com. Source data are provided as a Source Data file.
Article Snippet: Opt-SNE plots were generated using standard settings in
Techniques: Injection, Expressing, Luciferase, Quantitative RT-PCR, Isolation, Flow Cytometry, Software, Generated