gene expression chip Search Results


human stub1 gene orf cdna clone expression plasmid, c-gfpspark tag  (Sino Biological)
N/A
Full length Clone DNA of Human STIP1 homology and U-box containing protein 1, E3 ubiquitin protein ligase with C terminal GFPSpark tag.
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lentiviral gfp chip  (Sino Biological)
90
Sino Biological lentiviral gfp chip
<t>CHIP</t> targets HG induced‐PTEN for ubiquitin‐mediated proteasomal degradation cooperated by HSP70 under HG conditions. (a–c) Cells transfected with HA‐vector or HA‐CHIP in the presence and absence of MG‐132 for 6 h were subjected to HG challenge for 24 h. Whole cell lysate was immunoprecipitated with the anti‐HA, anti‐CHIP, and anti‐PTEN antibody, and subsequently immunoblotted with the primary antibodies, including anti‐HA, anti‐PTEN, and anti‐ubiquitin. (d, e) Cells transfected with HA‐vector, HA‐CHIP, and CHIP mutants (K30A, an H260Q) were treated with or without MG‐132 for 6 h in the presence of HG for 24 h. Whole cell lysate was immunoprecipitated with the anti‐HA and anti‐PTEN antibody followed by immunoblotting with the anti‐HA, anti‐PTEN, and anti‐ubiquitin antibody. (f) Cells were transfected with increasing concentrations of siHSP70 (10, 20, 30 nM) after challenged with HG for 24 h, and the expression level of PTEN and HSP70 was measured employing Western blot analysis. (g) Following cotransfection of <t>GFP‐vector</t> or GFP‐CHIP with increasing concentration of siHSP70 in WJMSCs were challenged with HG for 24 h, and the protein expression was measured via immunoblotting. (h) WJMSCs transfected with sicontrol, CHIP siRNA, or siHSP70 were subjected to HG challenge for 24 h, and the total cell extract was immunoblotted with CHIP, PTEN, and HSP70. β‐actin served as a loading control. (i) Docking studies demonstrating the molecular interaction of HSP70 with PTEN forming a heteromer complex (HSP70 and PTEN shown in quaternary structure with helices and sheets in complex). Values shown are mean ± SD. Quantification of the results are shown ( n = 3). *p < 0.05, **p < 0.01, and ***p < 0.001 indicates the significant difference. CHIP, carboxyl terminus of Hsc70 interacting protein; HG, high glucose; PTEN, phosphatase and tensin homolog; WJMSCs, Wharton's jelly derived mesenchymal stem cells
Lentiviral Gfp Chip, supplied by Sino Biological, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gene+expression+chip/Rat+STUB1+Gene+Lentiviral+ORF+cDNA+expression+plasmid%2C+C-GFPSpark+tag/pmc08459600-229-0-6
Average 90 stars, based on 1 article reviews
lentiviral gfp chip - by Bioz Stars, 2026-09
90/100 stars
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flag stub1  (Sino Biological)
93
Sino Biological flag stub1
<t>CHIP</t> targets HG induced‐PTEN for ubiquitin‐mediated proteasomal degradation cooperated by HSP70 under HG conditions. (a–c) Cells transfected with HA‐vector or HA‐CHIP in the presence and absence of MG‐132 for 6 h were subjected to HG challenge for 24 h. Whole cell lysate was immunoprecipitated with the anti‐HA, anti‐CHIP, and anti‐PTEN antibody, and subsequently immunoblotted with the primary antibodies, including anti‐HA, anti‐PTEN, and anti‐ubiquitin. (d, e) Cells transfected with HA‐vector, HA‐CHIP, and CHIP mutants (K30A, an H260Q) were treated with or without MG‐132 for 6 h in the presence of HG for 24 h. Whole cell lysate was immunoprecipitated with the anti‐HA and anti‐PTEN antibody followed by immunoblotting with the anti‐HA, anti‐PTEN, and anti‐ubiquitin antibody. (f) Cells were transfected with increasing concentrations of siHSP70 (10, 20, 30 nM) after challenged with HG for 24 h, and the expression level of PTEN and HSP70 was measured employing Western blot analysis. (g) Following cotransfection of <t>GFP‐vector</t> or GFP‐CHIP with increasing concentration of siHSP70 in WJMSCs were challenged with HG for 24 h, and the protein expression was measured via immunoblotting. (h) WJMSCs transfected with sicontrol, CHIP siRNA, or siHSP70 were subjected to HG challenge for 24 h, and the total cell extract was immunoblotted with CHIP, PTEN, and HSP70. β‐actin served as a loading control. (i) Docking studies demonstrating the molecular interaction of HSP70 with PTEN forming a heteromer complex (HSP70 and PTEN shown in quaternary structure with helices and sheets in complex). Values shown are mean ± SD. Quantification of the results are shown ( n = 3). *p < 0.05, **p < 0.01, and ***p < 0.001 indicates the significant difference. CHIP, carboxyl terminus of Hsc70 interacting protein; HG, high glucose; PTEN, phosphatase and tensin homolog; WJMSCs, Wharton's jelly derived mesenchymal stem cells
Flag Stub1, supplied by Sino Biological, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gene+expression+chip/Human+STUB1+Gene+ORF+cDNA+clone+expression+plasmid%2C+N-Flag+tag/pmc12625700-52-7-10
Average 93 stars, based on 1 article reviews
flag stub1 - by Bioz Stars, 2026-09
93/100 stars
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pcmv3 stub1 myc 37kd  (Sino Biological)
93
Sino Biological pcmv3 stub1 myc 37kd
<t>CHIP</t> targets HG induced‐PTEN for ubiquitin‐mediated proteasomal degradation cooperated by HSP70 under HG conditions. (a–c) Cells transfected with HA‐vector or HA‐CHIP in the presence and absence of MG‐132 for 6 h were subjected to HG challenge for 24 h. Whole cell lysate was immunoprecipitated with the anti‐HA, anti‐CHIP, and anti‐PTEN antibody, and subsequently immunoblotted with the primary antibodies, including anti‐HA, anti‐PTEN, and anti‐ubiquitin. (d, e) Cells transfected with HA‐vector, HA‐CHIP, and CHIP mutants (K30A, an H260Q) were treated with or without MG‐132 for 6 h in the presence of HG for 24 h. Whole cell lysate was immunoprecipitated with the anti‐HA and anti‐PTEN antibody followed by immunoblotting with the anti‐HA, anti‐PTEN, and anti‐ubiquitin antibody. (f) Cells were transfected with increasing concentrations of siHSP70 (10, 20, 30 nM) after challenged with HG for 24 h, and the expression level of PTEN and HSP70 was measured employing Western blot analysis. (g) Following cotransfection of <t>GFP‐vector</t> or GFP‐CHIP with increasing concentration of siHSP70 in WJMSCs were challenged with HG for 24 h, and the protein expression was measured via immunoblotting. (h) WJMSCs transfected with sicontrol, CHIP siRNA, or siHSP70 were subjected to HG challenge for 24 h, and the total cell extract was immunoblotted with CHIP, PTEN, and HSP70. β‐actin served as a loading control. (i) Docking studies demonstrating the molecular interaction of HSP70 with PTEN forming a heteromer complex (HSP70 and PTEN shown in quaternary structure with helices and sheets in complex). Values shown are mean ± SD. Quantification of the results are shown ( n = 3). *p < 0.05, **p < 0.01, and ***p < 0.001 indicates the significant difference. CHIP, carboxyl terminus of Hsc70 interacting protein; HG, high glucose; PTEN, phosphatase and tensin homolog; WJMSCs, Wharton's jelly derived mesenchymal stem cells
Pcmv3 Stub1 Myc 37kd, supplied by Sino Biological, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gene+expression+chip/Human+STUB1+Gene+ORF+cDNA+clone+expression+plasmid%2C+C-Myc+tag/pm38621458-46-21-26
Average 93 stars, based on 1 article reviews
pcmv3 stub1 myc 37kd - by Bioz Stars, 2026-09
93/100 stars
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gene expression microarray experiments  (DNA Chip Research Inc)
90
DNA Chip Research Inc gene expression microarray experiments
<t>CHIP</t> targets HG induced‐PTEN for ubiquitin‐mediated proteasomal degradation cooperated by HSP70 under HG conditions. (a–c) Cells transfected with HA‐vector or HA‐CHIP in the presence and absence of MG‐132 for 6 h were subjected to HG challenge for 24 h. Whole cell lysate was immunoprecipitated with the anti‐HA, anti‐CHIP, and anti‐PTEN antibody, and subsequently immunoblotted with the primary antibodies, including anti‐HA, anti‐PTEN, and anti‐ubiquitin. (d, e) Cells transfected with HA‐vector, HA‐CHIP, and CHIP mutants (K30A, an H260Q) were treated with or without MG‐132 for 6 h in the presence of HG for 24 h. Whole cell lysate was immunoprecipitated with the anti‐HA and anti‐PTEN antibody followed by immunoblotting with the anti‐HA, anti‐PTEN, and anti‐ubiquitin antibody. (f) Cells were transfected with increasing concentrations of siHSP70 (10, 20, 30 nM) after challenged with HG for 24 h, and the expression level of PTEN and HSP70 was measured employing Western blot analysis. (g) Following cotransfection of <t>GFP‐vector</t> or GFP‐CHIP with increasing concentration of siHSP70 in WJMSCs were challenged with HG for 24 h, and the protein expression was measured via immunoblotting. (h) WJMSCs transfected with sicontrol, CHIP siRNA, or siHSP70 were subjected to HG challenge for 24 h, and the total cell extract was immunoblotted with CHIP, PTEN, and HSP70. β‐actin served as a loading control. (i) Docking studies demonstrating the molecular interaction of HSP70 with PTEN forming a heteromer complex (HSP70 and PTEN shown in quaternary structure with helices and sheets in complex). Values shown are mean ± SD. Quantification of the results are shown ( n = 3). *p < 0.05, **p < 0.01, and ***p < 0.001 indicates the significant difference. CHIP, carboxyl terminus of Hsc70 interacting protein; HG, high glucose; PTEN, phosphatase and tensin homolog; WJMSCs, Wharton's jelly derived mesenchymal stem cells
Gene Expression Microarray Experiments, supplied by DNA Chip Research Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gene+expression+chip/gene+expression+microarray+experiments/pm38169039-67-112-121
Average 90 stars, based on 1 article reviews
gene expression microarray experiments - by Bioz Stars, 2026-09
90/100 stars
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gene expression microarray  (WholeGenome LLC)
90
WholeGenome LLC gene expression microarray
<t>CHIP</t> targets HG induced‐PTEN for ubiquitin‐mediated proteasomal degradation cooperated by HSP70 under HG conditions. (a–c) Cells transfected with HA‐vector or HA‐CHIP in the presence and absence of MG‐132 for 6 h were subjected to HG challenge for 24 h. Whole cell lysate was immunoprecipitated with the anti‐HA, anti‐CHIP, and anti‐PTEN antibody, and subsequently immunoblotted with the primary antibodies, including anti‐HA, anti‐PTEN, and anti‐ubiquitin. (d, e) Cells transfected with HA‐vector, HA‐CHIP, and CHIP mutants (K30A, an H260Q) were treated with or without MG‐132 for 6 h in the presence of HG for 24 h. Whole cell lysate was immunoprecipitated with the anti‐HA and anti‐PTEN antibody followed by immunoblotting with the anti‐HA, anti‐PTEN, and anti‐ubiquitin antibody. (f) Cells were transfected with increasing concentrations of siHSP70 (10, 20, 30 nM) after challenged with HG for 24 h, and the expression level of PTEN and HSP70 was measured employing Western blot analysis. (g) Following cotransfection of <t>GFP‐vector</t> or GFP‐CHIP with increasing concentration of siHSP70 in WJMSCs were challenged with HG for 24 h, and the protein expression was measured via immunoblotting. (h) WJMSCs transfected with sicontrol, CHIP siRNA, or siHSP70 were subjected to HG challenge for 24 h, and the total cell extract was immunoblotted with CHIP, PTEN, and HSP70. β‐actin served as a loading control. (i) Docking studies demonstrating the molecular interaction of HSP70 with PTEN forming a heteromer complex (HSP70 and PTEN shown in quaternary structure with helices and sheets in complex). Values shown are mean ± SD. Quantification of the results are shown ( n = 3). *p < 0.05, **p < 0.01, and ***p < 0.001 indicates the significant difference. CHIP, carboxyl terminus of Hsc70 interacting protein; HG, high glucose; PTEN, phosphatase and tensin homolog; WJMSCs, Wharton's jelly derived mesenchymal stem cells
Gene Expression Microarray, supplied by WholeGenome LLC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gene+expression+chip/gene+expression+chip/pm18514480-72-1-24
Average 90 stars, based on 1 article reviews
gene expression microarray - by Bioz Stars, 2026-09
90/100 stars
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gene expression microarray  (DNA Chip Research Inc)
90
DNA Chip Research Inc gene expression microarray
Comparison of global gene expression in trisomy 12 hPSC lines. ( A ) Cluster dendrogram of <t>microarray</t> data from trisomy 12 hPSC lines and their original lines. N = 3 from each lines. ( B–D ) Scatter plot of signal intensity for all microarray probes. Each dot in the plot shows the mean signal intensity of each probe averaged from 3 samples of H9 ( X-axis ) and H9(+ 12) ( Y-axis ) hESC lines ( B ), 201B7 ( X-axis ) and 201B7(+ 12) ( Y-axis ) hiPSC lines ( C ), and 19–9-7 T ( X-axis ) and 19–9-7 T(+ 12) ( Y-axis ) hiPSC lines ( D ). ( E , F ) Pie charts of significantly upregulated ( E ) or downregulated ( F ) probes of the trisomy 12 hPSC lines in common from microarray analysis (FDR < 0.1). ( G , H ) Pie charts of significantly upregulated G or downregulated H probes of the trisomy 12 hPSC lines in common from microarray analysis (FDR < 0.1). The area in blue indicates the ratio of the probes targeting chromosome 12. The area in red indicates the probes targeting the other chromosomes. ( I , J ) The list of “PANTHER” pathways and their p values extracted from the commonly upregulated genes I and downregulated gene J.
Gene Expression Microarray, supplied by DNA Chip Research Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gene+expression+chip/gene+expression+microarray/pmc11126453-57-0-7
Average 90 stars, based on 1 article reviews
gene expression microarray - by Bioz Stars, 2026-09
90/100 stars
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targeted gene capture chip  (BGI Shenzhen)
90
BGI Shenzhen targeted gene capture chip
Comparison of global gene expression in trisomy 12 hPSC lines. ( A ) Cluster dendrogram of <t>microarray</t> data from trisomy 12 hPSC lines and their original lines. N = 3 from each lines. ( B–D ) Scatter plot of signal intensity for all microarray probes. Each dot in the plot shows the mean signal intensity of each probe averaged from 3 samples of H9 ( X-axis ) and H9(+ 12) ( Y-axis ) hESC lines ( B ), 201B7 ( X-axis ) and 201B7(+ 12) ( Y-axis ) hiPSC lines ( C ), and 19–9-7 T ( X-axis ) and 19–9-7 T(+ 12) ( Y-axis ) hiPSC lines ( D ). ( E , F ) Pie charts of significantly upregulated ( E ) or downregulated ( F ) probes of the trisomy 12 hPSC lines in common from microarray analysis (FDR < 0.1). ( G , H ) Pie charts of significantly upregulated G or downregulated H probes of the trisomy 12 hPSC lines in common from microarray analysis (FDR < 0.1). The area in blue indicates the ratio of the probes targeting chromosome 12. The area in red indicates the probes targeting the other chromosomes. ( I , J ) The list of “PANTHER” pathways and their p values extracted from the commonly upregulated genes I and downregulated gene J.
Targeted Gene Capture Chip, supplied by BGI Shenzhen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gene+expression+chip/gene+expression+profile+chip/pmc04363838-43-42-47
Average 90 stars, based on 1 article reviews
targeted gene capture chip - by Bioz Stars, 2026-09
90/100 stars
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human stub1 gene orf cdna clone expression plasmid, c-myc tag  (Sino Biological)
93
Sino Biological human stub1 gene orf cdna clone expression plasmid, c-myc tag
Comparison of global gene expression in trisomy 12 hPSC lines. ( A ) Cluster dendrogram of <t>microarray</t> data from trisomy 12 hPSC lines and their original lines. N = 3 from each lines. ( B–D ) Scatter plot of signal intensity for all microarray probes. Each dot in the plot shows the mean signal intensity of each probe averaged from 3 samples of H9 ( X-axis ) and H9(+ 12) ( Y-axis ) hESC lines ( B ), 201B7 ( X-axis ) and 201B7(+ 12) ( Y-axis ) hiPSC lines ( C ), and 19–9-7 T ( X-axis ) and 19–9-7 T(+ 12) ( Y-axis ) hiPSC lines ( D ). ( E , F ) Pie charts of significantly upregulated ( E ) or downregulated ( F ) probes of the trisomy 12 hPSC lines in common from microarray analysis (FDR < 0.1). ( G , H ) Pie charts of significantly upregulated G or downregulated H probes of the trisomy 12 hPSC lines in common from microarray analysis (FDR < 0.1). The area in blue indicates the ratio of the probes targeting chromosome 12. The area in red indicates the probes targeting the other chromosomes. ( I , J ) The list of “PANTHER” pathways and their p values extracted from the commonly upregulated genes I and downregulated gene J.
Human Stub1 Gene Orf Cdna Clone Expression Plasmid, C Myc Tag, supplied by Sino Biological, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gene+expression+chip/Human+STUB1+Gene+ORF+cDNA+clone+expression+plasmid%2C+C-Myc+tag/custom%40hg12496-cm%4038621458
Average 93 stars, based on 1 article reviews
human stub1 gene orf cdna clone expression plasmid, c-myc tag - by Bioz Stars, 2026-09
93/100 stars
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rat stub1 gene lentiviral orf cdna expression plasmid, c-gfpspark tag  (Sino Biological)
90
Sino Biological rat stub1 gene lentiviral orf cdna expression plasmid, c-gfpspark tag
Comparison of global gene expression in trisomy 12 hPSC lines. ( A ) Cluster dendrogram of <t>microarray</t> data from trisomy 12 hPSC lines and their original lines. N = 3 from each lines. ( B–D ) Scatter plot of signal intensity for all microarray probes. Each dot in the plot shows the mean signal intensity of each probe averaged from 3 samples of H9 ( X-axis ) and H9(+ 12) ( Y-axis ) hESC lines ( B ), 201B7 ( X-axis ) and 201B7(+ 12) ( Y-axis ) hiPSC lines ( C ), and 19–9-7 T ( X-axis ) and 19–9-7 T(+ 12) ( Y-axis ) hiPSC lines ( D ). ( E , F ) Pie charts of significantly upregulated ( E ) or downregulated ( F ) probes of the trisomy 12 hPSC lines in common from microarray analysis (FDR < 0.1). ( G , H ) Pie charts of significantly upregulated G or downregulated H probes of the trisomy 12 hPSC lines in common from microarray analysis (FDR < 0.1). The area in blue indicates the ratio of the probes targeting chromosome 12. The area in red indicates the probes targeting the other chromosomes. ( I , J ) The list of “PANTHER” pathways and their p values extracted from the commonly upregulated genes I and downregulated gene J.
Rat Stub1 Gene Lentiviral Orf Cdna Expression Plasmid, C Gfpspark Tag, supplied by Sino Biological, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gene+expression+chip/Rat+STUB1+Gene+Lentiviral+ORF+cDNA+expression+plasmid%2C+C-GFPSpark+tag/custom%40rg83573-acgln%4010%2E21203%2Frs%2E3%2Ers-165217
Average 90 stars, based on 1 article reviews
rat stub1 gene lentiviral orf cdna expression plasmid, c-gfpspark tag - by Bioz Stars, 2026-09
90/100 stars
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gene expression chip moa v2.1 mouse onearray  (Phalanx Biotech)
90
Phalanx Biotech gene expression chip moa v2.1 mouse onearray
Comparison of global gene expression in trisomy 12 hPSC lines. ( A ) Cluster dendrogram of <t>microarray</t> data from trisomy 12 hPSC lines and their original lines. N = 3 from each lines. ( B–D ) Scatter plot of signal intensity for all microarray probes. Each dot in the plot shows the mean signal intensity of each probe averaged from 3 samples of H9 ( X-axis ) and H9(+ 12) ( Y-axis ) hESC lines ( B ), 201B7 ( X-axis ) and 201B7(+ 12) ( Y-axis ) hiPSC lines ( C ), and 19–9-7 T ( X-axis ) and 19–9-7 T(+ 12) ( Y-axis ) hiPSC lines ( D ). ( E , F ) Pie charts of significantly upregulated ( E ) or downregulated ( F ) probes of the trisomy 12 hPSC lines in common from microarray analysis (FDR < 0.1). ( G , H ) Pie charts of significantly upregulated G or downregulated H probes of the trisomy 12 hPSC lines in common from microarray analysis (FDR < 0.1). The area in blue indicates the ratio of the probes targeting chromosome 12. The area in red indicates the probes targeting the other chromosomes. ( I , J ) The list of “PANTHER” pathways and their p values extracted from the commonly upregulated genes I and downregulated gene J.
Gene Expression Chip Moa V2.1 Mouse Onearray, supplied by Phalanx Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gene+expression+chip/gene+expression+chip+moa+v2+1+mouse+onearray/pm24309110-60-19-9
Average 90 stars, based on 1 article reviews
gene expression chip moa v2.1 mouse onearray - by Bioz Stars, 2026-09
90/100 stars
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global gene expression analysis (rna isolation, cdna synthesis, hybridization, and chip analysis)  (Gene Logic Inc)
90
Gene Logic Inc global gene expression analysis (rna isolation, cdna synthesis, hybridization, and chip analysis)
Comparison of global gene expression in trisomy 12 hPSC lines. ( A ) Cluster dendrogram of <t>microarray</t> data from trisomy 12 hPSC lines and their original lines. N = 3 from each lines. ( B–D ) Scatter plot of signal intensity for all microarray probes. Each dot in the plot shows the mean signal intensity of each probe averaged from 3 samples of H9 ( X-axis ) and H9(+ 12) ( Y-axis ) hESC lines ( B ), 201B7 ( X-axis ) and 201B7(+ 12) ( Y-axis ) hiPSC lines ( C ), and 19–9-7 T ( X-axis ) and 19–9-7 T(+ 12) ( Y-axis ) hiPSC lines ( D ). ( E , F ) Pie charts of significantly upregulated ( E ) or downregulated ( F ) probes of the trisomy 12 hPSC lines in common from microarray analysis (FDR < 0.1). ( G , H ) Pie charts of significantly upregulated G or downregulated H probes of the trisomy 12 hPSC lines in common from microarray analysis (FDR < 0.1). The area in blue indicates the ratio of the probes targeting chromosome 12. The area in red indicates the probes targeting the other chromosomes. ( I , J ) The list of “PANTHER” pathways and their p values extracted from the commonly upregulated genes I and downregulated gene J.
Global Gene Expression Analysis (Rna Isolation, Cdna Synthesis, Hybridization, And Chip Analysis), supplied by Gene Logic Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gene+expression+chip/global+gene+expression+analysis++rna+isolation++cdna+synthesis++hybridization++and+chip+analysis+/pm16540726-57-0-15
Average 90 stars, based on 1 article reviews
global gene expression analysis (rna isolation, cdna synthesis, hybridization, and chip analysis) - by Bioz Stars, 2026-09
90/100 stars
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Image Search Results


CHIP targets HG induced‐PTEN for ubiquitin‐mediated proteasomal degradation cooperated by HSP70 under HG conditions. (a–c) Cells transfected with HA‐vector or HA‐CHIP in the presence and absence of MG‐132 for 6 h were subjected to HG challenge for 24 h. Whole cell lysate was immunoprecipitated with the anti‐HA, anti‐CHIP, and anti‐PTEN antibody, and subsequently immunoblotted with the primary antibodies, including anti‐HA, anti‐PTEN, and anti‐ubiquitin. (d, e) Cells transfected with HA‐vector, HA‐CHIP, and CHIP mutants (K30A, an H260Q) were treated with or without MG‐132 for 6 h in the presence of HG for 24 h. Whole cell lysate was immunoprecipitated with the anti‐HA and anti‐PTEN antibody followed by immunoblotting with the anti‐HA, anti‐PTEN, and anti‐ubiquitin antibody. (f) Cells were transfected with increasing concentrations of siHSP70 (10, 20, 30 nM) after challenged with HG for 24 h, and the expression level of PTEN and HSP70 was measured employing Western blot analysis. (g) Following cotransfection of GFP‐vector or GFP‐CHIP with increasing concentration of siHSP70 in WJMSCs were challenged with HG for 24 h, and the protein expression was measured via immunoblotting. (h) WJMSCs transfected with sicontrol, CHIP siRNA, or siHSP70 were subjected to HG challenge for 24 h, and the total cell extract was immunoblotted with CHIP, PTEN, and HSP70. β‐actin served as a loading control. (i) Docking studies demonstrating the molecular interaction of HSP70 with PTEN forming a heteromer complex (HSP70 and PTEN shown in quaternary structure with helices and sheets in complex). Values shown are mean ± SD. Quantification of the results are shown ( n = 3). *p < 0.05, **p < 0.01, and ***p < 0.001 indicates the significant difference. CHIP, carboxyl terminus of Hsc70 interacting protein; HG, high glucose; PTEN, phosphatase and tensin homolog; WJMSCs, Wharton's jelly derived mesenchymal stem cells

Journal: Bioengineering & Translational Medicine

Article Title: E3 ligase activity of Carboxyl terminus of Hsc70 interacting protein ( CHIP) in Wharton's jelly derived mesenchymal stem cells improves their persistence under hyperglycemic stress and promotes the prophylactic effects against diabetic cardiac damages

doi: 10.1002/btm2.10234

Figure Lengend Snippet: CHIP targets HG induced‐PTEN for ubiquitin‐mediated proteasomal degradation cooperated by HSP70 under HG conditions. (a–c) Cells transfected with HA‐vector or HA‐CHIP in the presence and absence of MG‐132 for 6 h were subjected to HG challenge for 24 h. Whole cell lysate was immunoprecipitated with the anti‐HA, anti‐CHIP, and anti‐PTEN antibody, and subsequently immunoblotted with the primary antibodies, including anti‐HA, anti‐PTEN, and anti‐ubiquitin. (d, e) Cells transfected with HA‐vector, HA‐CHIP, and CHIP mutants (K30A, an H260Q) were treated with or without MG‐132 for 6 h in the presence of HG for 24 h. Whole cell lysate was immunoprecipitated with the anti‐HA and anti‐PTEN antibody followed by immunoblotting with the anti‐HA, anti‐PTEN, and anti‐ubiquitin antibody. (f) Cells were transfected with increasing concentrations of siHSP70 (10, 20, 30 nM) after challenged with HG for 24 h, and the expression level of PTEN and HSP70 was measured employing Western blot analysis. (g) Following cotransfection of GFP‐vector or GFP‐CHIP with increasing concentration of siHSP70 in WJMSCs were challenged with HG for 24 h, and the protein expression was measured via immunoblotting. (h) WJMSCs transfected with sicontrol, CHIP siRNA, or siHSP70 were subjected to HG challenge for 24 h, and the total cell extract was immunoblotted with CHIP, PTEN, and HSP70. β‐actin served as a loading control. (i) Docking studies demonstrating the molecular interaction of HSP70 with PTEN forming a heteromer complex (HSP70 and PTEN shown in quaternary structure with helices and sheets in complex). Values shown are mean ± SD. Quantification of the results are shown ( n = 3). *p < 0.05, **p < 0.01, and ***p < 0.001 indicates the significant difference. CHIP, carboxyl terminus of Hsc70 interacting protein; HG, high glucose; PTEN, phosphatase and tensin homolog; WJMSCs, Wharton's jelly derived mesenchymal stem cells

Article Snippet: Lentiviral GFP‐CHIP was purchased from the Sino biological (RG83573‐ACGLN), while the lentiviral expressing small hairpin RNAs (shRNAs), including shcontrol (pLAS.Void), shCHIP (TRCN0000007528 NM_005861), shPTEN (TRCN0000002746 NM_000314), and lentiviral packaging plasmids (pCMVΔR8.91 and pMD.G), were obtained from the national RNAi core facility (Academia Sinica).

Techniques: Transfection, Plasmid Preparation, Immunoprecipitation, Western Blot, Expressing, Cotransfection, Concentration Assay, Derivative Assay

CHIP overexpressed WJMSCs rescued hyperglycemic effects under diabetic conditions. (a) Schematic illustration of STZ‐induced diabetes, and WJMSCs administration expressing different plasmids, including GFP‐CHIP, shCHIP, and shPTEN. (b) The oral glucose tolerance test (OGTT) was performed after 6 weeks treatment for the indicated time points (0, 30, 60, 90, and 120 min) in various experimental groups, including control, STZ‐induced diabetes (STZ), STZ‐induced diabetes administered with WJMSCs alone (STZ + WJMSCs), STZ‐induced diabetes injected with CHIP overexpressed WJMSCs (STZ + CHIP‐WJMSCs), STZ‐induced diabetes transplanted with CHIP knockdown WJMSCs (STZ + shCHIP‐WJMSCs), and STZ‐induced diabetic rats infused with PTEN knockdown WJMSCs (STZ + shPTEN‐WJMSCs) after the rats were fasted for 14 h. (c) Morphological assessment of cardiac tissues in different experimental groups. (d) Echocardiographic evaluation of cardiac function in different experimental groups (control, STZ, STZ + WJMSCs, STZ + CHIP‐WJMSCs, STZ + shCHIP‐WJMSCs, and STZ + shPTEN‐WJMSCs). (e) Total cell lysate from the left ventricle was quantified and measured using Western blot. Protein expression levels of the apoptosis (p‐AKT, Bax, and Cyt‐c) and oxidative stress markers (catalase, SOD2, and gp91 PHOX ) were assessed. GAPDH act as a loading control. Values shown are mean ± SD. Quantification of the results are shown ( n = 3). *p < 0.05, **p < 0.01, and ***p < 0.001 shows the significance. CHIP, carboxyl terminus of Hsc70 interacting protein; GAPDH, Glyceraldehyde‐3‐phosphate dehydrogenase; PTEN, phosphatase and tensin homolog; STZ, streptozotocin; WJMSCs, Wharton's jelly derived mesenchymal stem cells

Journal: Bioengineering & Translational Medicine

Article Title: E3 ligase activity of Carboxyl terminus of Hsc70 interacting protein ( CHIP) in Wharton's jelly derived mesenchymal stem cells improves their persistence under hyperglycemic stress and promotes the prophylactic effects against diabetic cardiac damages

doi: 10.1002/btm2.10234

Figure Lengend Snippet: CHIP overexpressed WJMSCs rescued hyperglycemic effects under diabetic conditions. (a) Schematic illustration of STZ‐induced diabetes, and WJMSCs administration expressing different plasmids, including GFP‐CHIP, shCHIP, and shPTEN. (b) The oral glucose tolerance test (OGTT) was performed after 6 weeks treatment for the indicated time points (0, 30, 60, 90, and 120 min) in various experimental groups, including control, STZ‐induced diabetes (STZ), STZ‐induced diabetes administered with WJMSCs alone (STZ + WJMSCs), STZ‐induced diabetes injected with CHIP overexpressed WJMSCs (STZ + CHIP‐WJMSCs), STZ‐induced diabetes transplanted with CHIP knockdown WJMSCs (STZ + shCHIP‐WJMSCs), and STZ‐induced diabetic rats infused with PTEN knockdown WJMSCs (STZ + shPTEN‐WJMSCs) after the rats were fasted for 14 h. (c) Morphological assessment of cardiac tissues in different experimental groups. (d) Echocardiographic evaluation of cardiac function in different experimental groups (control, STZ, STZ + WJMSCs, STZ + CHIP‐WJMSCs, STZ + shCHIP‐WJMSCs, and STZ + shPTEN‐WJMSCs). (e) Total cell lysate from the left ventricle was quantified and measured using Western blot. Protein expression levels of the apoptosis (p‐AKT, Bax, and Cyt‐c) and oxidative stress markers (catalase, SOD2, and gp91 PHOX ) were assessed. GAPDH act as a loading control. Values shown are mean ± SD. Quantification of the results are shown ( n = 3). *p < 0.05, **p < 0.01, and ***p < 0.001 shows the significance. CHIP, carboxyl terminus of Hsc70 interacting protein; GAPDH, Glyceraldehyde‐3‐phosphate dehydrogenase; PTEN, phosphatase and tensin homolog; STZ, streptozotocin; WJMSCs, Wharton's jelly derived mesenchymal stem cells

Article Snippet: Lentiviral GFP‐CHIP was purchased from the Sino biological (RG83573‐ACGLN), while the lentiviral expressing small hairpin RNAs (shRNAs), including shcontrol (pLAS.Void), shCHIP (TRCN0000007528 NM_005861), shPTEN (TRCN0000002746 NM_000314), and lentiviral packaging plasmids (pCMVΔR8.91 and pMD.G), were obtained from the national RNAi core facility (Academia Sinica).

Techniques: Expressing, Injection, Western Blot, Derivative Assay

Comparison of global gene expression in trisomy 12 hPSC lines. ( A ) Cluster dendrogram of microarray data from trisomy 12 hPSC lines and their original lines. N = 3 from each lines. ( B–D ) Scatter plot of signal intensity for all microarray probes. Each dot in the plot shows the mean signal intensity of each probe averaged from 3 samples of H9 ( X-axis ) and H9(+ 12) ( Y-axis ) hESC lines ( B ), 201B7 ( X-axis ) and 201B7(+ 12) ( Y-axis ) hiPSC lines ( C ), and 19–9-7 T ( X-axis ) and 19–9-7 T(+ 12) ( Y-axis ) hiPSC lines ( D ). ( E , F ) Pie charts of significantly upregulated ( E ) or downregulated ( F ) probes of the trisomy 12 hPSC lines in common from microarray analysis (FDR < 0.1). ( G , H ) Pie charts of significantly upregulated G or downregulated H probes of the trisomy 12 hPSC lines in common from microarray analysis (FDR < 0.1). The area in blue indicates the ratio of the probes targeting chromosome 12. The area in red indicates the probes targeting the other chromosomes. ( I , J ) The list of “PANTHER” pathways and their p values extracted from the commonly upregulated genes I and downregulated gene J.

Journal: In Vitro Cellular & Developmental Biology. Animal

Article Title: Trisomy 12 compromises the mesendodermal differentiation propensity of human pluripotent stem cells

doi: 10.1007/s11626-023-00824-9

Figure Lengend Snippet: Comparison of global gene expression in trisomy 12 hPSC lines. ( A ) Cluster dendrogram of microarray data from trisomy 12 hPSC lines and their original lines. N = 3 from each lines. ( B–D ) Scatter plot of signal intensity for all microarray probes. Each dot in the plot shows the mean signal intensity of each probe averaged from 3 samples of H9 ( X-axis ) and H9(+ 12) ( Y-axis ) hESC lines ( B ), 201B7 ( X-axis ) and 201B7(+ 12) ( Y-axis ) hiPSC lines ( C ), and 19–9-7 T ( X-axis ) and 19–9-7 T(+ 12) ( Y-axis ) hiPSC lines ( D ). ( E , F ) Pie charts of significantly upregulated ( E ) or downregulated ( F ) probes of the trisomy 12 hPSC lines in common from microarray analysis (FDR < 0.1). ( G , H ) Pie charts of significantly upregulated G or downregulated H probes of the trisomy 12 hPSC lines in common from microarray analysis (FDR < 0.1). The area in blue indicates the ratio of the probes targeting chromosome 12. The area in red indicates the probes targeting the other chromosomes. ( I , J ) The list of “PANTHER” pathways and their p values extracted from the commonly upregulated genes I and downregulated gene J.

Article Snippet: Gene expression microarray experiments were performed by DNA Chip Research Inc (Tokyo, Japan).

Techniques: Comparison, Gene Expression, Microarray