Journal: Scientific Reports

Article Title: The unexplored mechanism of antitumoral effect of pirfenidone in melanoma cells

doi: 10.1038/s41598-025-13584-1

Figure Lengend Snippet: Effect of PFD on furin dependent melanoma cell invasiveness and TGFβ production. ( A ) Inbibition of melanoma cell invasiveness in A375M6, and M21 human melanoma cells. Results are represented as a mean ± SD of tumor cells migrated for 5 h through GeltrexTM coated-filters. The results are presented as the mean ± SD of three independent experiments. ( B ) Western blotting of intracellular TGFβ (44 kDa) in A375M6, and M21 human melanoma exposed to PFD at 2.7 mM and 8.1 mM concentrations, in the presence or in the absence of BOS318. Each panel of the figure shows a typical experiment out of three that gave similar results. ( C ) Densitometric analysis is presented as the mean ± SD of the three independent experiments. Statistical analysis was performed using Graph Pad Prism 4 software and one way ANOVA test. Statistical significance was assumed for p values ≤ 0.05: * P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001.

Article Snippet: Melanoma cell invasiveness was determined in vitro on GeltrexTM -precoated polycarbonate inserts (Sarstedt 83.3932.800, 8 μm pore size, 0.3 cm 2 growth surface).

Techniques: Western Blot, Software