gcamp6f Search Results


aav1 hsyn gcampéf  (Addgene inc)
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nanoject  (Addgene inc)
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aav2  (Addgene inc)
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fast gcamp6f rs09  (Addgene inc)
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plasmid virus source packaging serotype titer paav ef1a fdiogcamp6f  (Addgene inc)
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gcamp6f  (Addgene inc)
90
Addgene inc gcamp6f
Figure 1. VIP and NDNF interneurons respond to sound but with less trial-to-trial reliability than pyramidal neurons (A) Schematic showing relative positions of PYR, VIP, and NDNF neuron populations in the cortex. (B) Simple and complex sound stimuli were passively presented to head-fixed mice running freely on a wheel during two-photon calcium imaging of <t>GCaMP6f</t> in the left auditory cortex. (C) Example fields of view from each neuronal population. Maximum projection images are shown to highlight differences in density between each population. (D) Example single-cell responses to single trials of white noise bursts (20 trials) highlighting trial-to-trial response variability. Left, responses to 20 trials of identical white noise burst stimuli. Middle, overlaid single-trial responses with average in bold. Right, heatmap of Pearson’s R calculated for each pair of trials (upper diagonal: sound trials, lower diagonal: blank trials). The average Pearson’s R across all sound trials is the reliability score (RSound) for that neuron. (E) Average reliability score (RSound) for each neuronal group. Single points denote mean reliability across auditory stimuli for a single neuron. Mixed-effects model one-way ANOVA with Tukey’s post hoc test (F2,2504 = 77.48, p < 0.001), ***p < 0.001, nPYR = 694 neurons, nVIP = 773 neurons, nNDNF = 829 neurons.
Gcamp6f, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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paav teto 3g gcamp6f wpre  (Addgene inc)
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Figure 1. VIP and NDNF interneurons respond to sound but with less trial-to-trial reliability than pyramidal neurons (A) Schematic showing relative positions of PYR, VIP, and NDNF neuron populations in the cortex. (B) Simple and complex sound stimuli were passively presented to head-fixed mice running freely on a wheel during two-photon calcium imaging of <t>GCaMP6f</t> in the left auditory cortex. (C) Example fields of view from each neuronal population. Maximum projection images are shown to highlight differences in density between each population. (D) Example single-cell responses to single trials of white noise bursts (20 trials) highlighting trial-to-trial response variability. Left, responses to 20 trials of identical white noise burst stimuli. Middle, overlaid single-trial responses with average in bold. Right, heatmap of Pearson’s R calculated for each pair of trials (upper diagonal: sound trials, lower diagonal: blank trials). The average Pearson’s R across all sound trials is the reliability score (RSound) for that neuron. (E) Average reliability score (RSound) for each neuronal group. Single points denote mean reliability across auditory stimuli for a single neuron. Mixed-effects model one-way ANOVA with Tukey’s post hoc test (F2,2504 = 77.48, p < 0.001), ***p < 0.001, nPYR = 694 neurons, nVIP = 773 neurons, nNDNF = 829 neurons.
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virus aav encodingflp dependentgcamp6f  (Addgene inc)
91
Addgene inc virus aav encodingflp dependentgcamp6f
Figure 1. VIP and NDNF interneurons respond to sound but with less trial-to-trial reliability than pyramidal neurons (A) Schematic showing relative positions of PYR, VIP, and NDNF neuron populations in the cortex. (B) Simple and complex sound stimuli were passively presented to head-fixed mice running freely on a wheel during two-photon calcium imaging of <t>GCaMP6f</t> in the left auditory cortex. (C) Example fields of view from each neuronal population. Maximum projection images are shown to highlight differences in density between each population. (D) Example single-cell responses to single trials of white noise bursts (20 trials) highlighting trial-to-trial response variability. Left, responses to 20 trials of identical white noise burst stimuli. Middle, overlaid single-trial responses with average in bold. Right, heatmap of Pearson’s R calculated for each pair of trials (upper diagonal: sound trials, lower diagonal: blank trials). The average Pearson’s R across all sound trials is the reliability score (RSound) for that neuron. (E) Average reliability score (RSound) for each neuronal group. Single points denote mean reliability across auditory stimuli for a single neuron. Mixed-effects model one-way ANOVA with Tukey’s post hoc test (F2,2504 = 77.48, p < 0.001), ***p < 0.001, nPYR = 694 neurons, nVIP = 773 neurons, nNDNF = 829 neurons.
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pzac2 1 gfaabc1d cyto gcamp6f  (Addgene inc)
95
Addgene inc pzac2 1 gfaabc1d cyto gcamp6f
Figure 1. VIP and NDNF interneurons respond to sound but with less trial-to-trial reliability than pyramidal neurons (A) Schematic showing relative positions of PYR, VIP, and NDNF neuron populations in the cortex. (B) Simple and complex sound stimuli were passively presented to head-fixed mice running freely on a wheel during two-photon calcium imaging of <t>GCaMP6f</t> in the left auditory cortex. (C) Example fields of view from each neuronal population. Maximum projection images are shown to highlight differences in density between each population. (D) Example single-cell responses to single trials of white noise bursts (20 trials) highlighting trial-to-trial response variability. Left, responses to 20 trials of identical white noise burst stimuli. Middle, overlaid single-trial responses with average in bold. Right, heatmap of Pearson’s R calculated for each pair of trials (upper diagonal: sound trials, lower diagonal: blank trials). The average Pearson’s R across all sound trials is the reliability score (RSound) for that neuron. (E) Average reliability score (RSound) for each neuronal group. Single points denote mean reliability across auditory stimuli for a single neuron. Mixed-effects model one-way ANOVA with Tukey’s post hoc test (F2,2504 = 77.48, p < 0.001), ***p < 0.001, nPYR = 694 neurons, nVIP = 773 neurons, nNDNF = 829 neurons.
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nci n87 cells  (Addgene inc)
94
Addgene inc nci n87 cells
Figure 1. VIP and NDNF interneurons respond to sound but with less trial-to-trial reliability than pyramidal neurons (A) Schematic showing relative positions of PYR, VIP, and NDNF neuron populations in the cortex. (B) Simple and complex sound stimuli were passively presented to head-fixed mice running freely on a wheel during two-photon calcium imaging of <t>GCaMP6f</t> in the left auditory cortex. (C) Example fields of view from each neuronal population. Maximum projection images are shown to highlight differences in density between each population. (D) Example single-cell responses to single trials of white noise bursts (20 trials) highlighting trial-to-trial response variability. Left, responses to 20 trials of identical white noise burst stimuli. Middle, overlaid single-trial responses with average in bold. Right, heatmap of Pearson’s R calculated for each pair of trials (upper diagonal: sound trials, lower diagonal: blank trials). The average Pearson’s R across all sound trials is the reliability score (RSound) for that neuron. (E) Average reliability score (RSound) for each neuronal group. Single points denote mean reliability across auditory stimuli for a single neuron. Mixed-effects model one-way ANOVA with Tukey’s post hoc test (F2,2504 = 77.48, p < 0.001), ***p < 0.001, nPYR = 694 neurons, nVIP = 773 neurons, nNDNF = 829 neurons.
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51085 aav1  (Addgene inc)
94
Addgene inc 51085 aav1

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Image Search Results


Figure 1. VIP and NDNF interneurons respond to sound but with less trial-to-trial reliability than pyramidal neurons (A) Schematic showing relative positions of PYR, VIP, and NDNF neuron populations in the cortex. (B) Simple and complex sound stimuli were passively presented to head-fixed mice running freely on a wheel during two-photon calcium imaging of GCaMP6f in the left auditory cortex. (C) Example fields of view from each neuronal population. Maximum projection images are shown to highlight differences in density between each population. (D) Example single-cell responses to single trials of white noise bursts (20 trials) highlighting trial-to-trial response variability. Left, responses to 20 trials of identical white noise burst stimuli. Middle, overlaid single-trial responses with average in bold. Right, heatmap of Pearson’s R calculated for each pair of trials (upper diagonal: sound trials, lower diagonal: blank trials). The average Pearson’s R across all sound trials is the reliability score (RSound) for that neuron. (E) Average reliability score (RSound) for each neuronal group. Single points denote mean reliability across auditory stimuli for a single neuron. Mixed-effects model one-way ANOVA with Tukey’s post hoc test (F2,2504 = 77.48, p < 0.001), ***p < 0.001, nPYR = 694 neurons, nVIP = 773 neurons, nNDNF = 829 neurons.

Journal: Cell reports

Article Title: Reliable sensory processing of superficial cortical interneurons is modulated by behavioral state.

doi: 10.1016/j.celrep.2025.115678

Figure Lengend Snippet: Figure 1. VIP and NDNF interneurons respond to sound but with less trial-to-trial reliability than pyramidal neurons (A) Schematic showing relative positions of PYR, VIP, and NDNF neuron populations in the cortex. (B) Simple and complex sound stimuli were passively presented to head-fixed mice running freely on a wheel during two-photon calcium imaging of GCaMP6f in the left auditory cortex. (C) Example fields of view from each neuronal population. Maximum projection images are shown to highlight differences in density between each population. (D) Example single-cell responses to single trials of white noise bursts (20 trials) highlighting trial-to-trial response variability. Left, responses to 20 trials of identical white noise burst stimuli. Middle, overlaid single-trial responses with average in bold. Right, heatmap of Pearson’s R calculated for each pair of trials (upper diagonal: sound trials, lower diagonal: blank trials). The average Pearson’s R across all sound trials is the reliability score (RSound) for that neuron. (E) Average reliability score (RSound) for each neuronal group. Single points denote mean reliability across auditory stimuli for a single neuron. Mixed-effects model one-way ANOVA with Tukey’s post hoc test (F2,2504 = 77.48, p < 0.001), ***p < 0.001, nPYR = 694 neurons, nVIP = 773 neurons, nNDNF = 829 neurons.

Article Snippet: To selectively express GCaMP6f in pyramidal neurons (PYR), Ai148-negative mice from both strains were injected with AAV9-CaMKII-GCaMP6f-WPRE-SV40 (2.7x10̂12 gc/ml, Addgene, 107790).

Techniques: Imaging

Journal: STAR Protocols

Article Title: Integrated functional neuronal network analysis of 3D silk-collagen scaffold-based mouse cortical culture

doi: 10.1016/j.xpro.2020.100292

Figure Lengend Snippet:

Article Snippet: AAV-hSyn1-GCaMP6f-P2A-nls-dTomato , Addgene , 51085-AAV1 https://www.addgene.org/51085/.

Techniques: Virus, Recombinant, Saline, Sterility, TaqMan Assay, Software, Molecular Weight, Dissection, Microscopy, Fluorescence, Spectrophotometry, cDNA Synthesis, Gene Expression