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  • 99
    Thermo Fisher high capacity cdna reverse transcription kit
    High Capacity Cdna Reverse Transcription Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 118175 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    high capacity cdna reverse transcription kit - by Bioz Stars, 2020-09
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    96
    Millipore faststart high fidelity pcr system
    Faststart High Fidelity Pcr System, supplied by Millipore, used in various techniques. Bioz Stars score: 96/100, based on 47 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 96 stars, based on 47 article reviews
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    99
    Thermo Fisher superscript iii reverse transcriptase
    Expression of BTV-8- <t>NS3,</t> VP2 and VP7 proteins from recombinant adenoviruses in Vero and ST cells. Fluorescence microscopy was used to analyse the expression of NS3, VP2 or VP7 proteins after infection with recombinant adenoviruses Ad5-BTV-NS3, Ad5-BTV-VP2 or Ad5-BTV-VP7. (A) Vero cells infected with Ad5-BTV-NS3, Ad5-BTV-VP2 or Ad5-BTV-VP7. (B) ST cells infected with Ad5-BTV-NS3, Ad5-BTV-VP2 or Ad5-BTV-VP7. The <t>three</t> panels shown from left to right are antibody staining against BTV-8 (green), DAPI staining (blue), and merge. The micrographs are representative of at least three independent experiments. Magnification 20X; bar graphs correspond to 50 nm.
    Superscript Iii Reverse Transcriptase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 91016 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher amplitaq gold dna polymerase
    Expression of BTV-8- <t>NS3,</t> VP2 and VP7 proteins from recombinant adenoviruses in Vero and ST cells. Fluorescence microscopy was used to analyse the expression of NS3, VP2 or VP7 proteins after infection with recombinant adenoviruses Ad5-BTV-NS3, Ad5-BTV-VP2 or Ad5-BTV-VP7. (A) Vero cells infected with Ad5-BTV-NS3, Ad5-BTV-VP2 or Ad5-BTV-VP7. (B) ST cells infected with Ad5-BTV-NS3, Ad5-BTV-VP2 or Ad5-BTV-VP7. The <t>three</t> panels shown from left to right are antibody staining against BTV-8 (green), DAPI staining (blue), and merge. The micrographs are representative of at least three independent experiments. Magnification 20X; bar graphs correspond to 50 nm.
    Amplitaq Gold Dna Polymerase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 13243 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Qiagen taq polymerase
    Expression of BTV-8- <t>NS3,</t> VP2 and VP7 proteins from recombinant adenoviruses in Vero and ST cells. Fluorescence microscopy was used to analyse the expression of NS3, VP2 or VP7 proteins after infection with recombinant adenoviruses Ad5-BTV-NS3, Ad5-BTV-VP2 or Ad5-BTV-VP7. (A) Vero cells infected with Ad5-BTV-NS3, Ad5-BTV-VP2 or Ad5-BTV-VP7. (B) ST cells infected with Ad5-BTV-NS3, Ad5-BTV-VP2 or Ad5-BTV-VP7. The <t>three</t> panels shown from left to right are antibody staining against BTV-8 (green), DAPI staining (blue), and merge. The micrographs are representative of at least three independent experiments. Magnification 20X; bar graphs correspond to 50 nm.
    Taq Polymerase, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 6398 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher taqman universal pcr master mix
    Expression of BTV-8- <t>NS3,</t> VP2 and VP7 proteins from recombinant adenoviruses in Vero and ST cells. Fluorescence microscopy was used to analyse the expression of NS3, VP2 or VP7 proteins after infection with recombinant adenoviruses Ad5-BTV-NS3, Ad5-BTV-VP2 or Ad5-BTV-VP7. (A) Vero cells infected with Ad5-BTV-NS3, Ad5-BTV-VP2 or Ad5-BTV-VP7. (B) ST cells infected with Ad5-BTV-NS3, Ad5-BTV-VP2 or Ad5-BTV-VP7. The <t>three</t> panels shown from left to right are antibody staining against BTV-8 (green), DAPI staining (blue), and merge. The micrographs are representative of at least three independent experiments. Magnification 20X; bar graphs correspond to 50 nm.
    Taqman Universal Pcr Master Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 54482 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher 3730xl dna analyzer
    Expression of BTV-8- <t>NS3,</t> VP2 and VP7 proteins from recombinant adenoviruses in Vero and ST cells. Fluorescence microscopy was used to analyse the expression of NS3, VP2 or VP7 proteins after infection with recombinant adenoviruses Ad5-BTV-NS3, Ad5-BTV-VP2 or Ad5-BTV-VP7. (A) Vero cells infected with Ad5-BTV-NS3, Ad5-BTV-VP2 or Ad5-BTV-VP7. (B) ST cells infected with Ad5-BTV-NS3, Ad5-BTV-VP2 or Ad5-BTV-VP7. The <t>three</t> panels shown from left to right are antibody staining against BTV-8 (green), DAPI staining (blue), and merge. The micrographs are representative of at least three independent experiments. Magnification 20X; bar graphs correspond to 50 nm.
    3730xl Dna Analyzer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 46651 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher applied biosystems dna analyzer
    Expression of BTV-8- <t>NS3,</t> VP2 and VP7 proteins from recombinant adenoviruses in Vero and ST cells. Fluorescence microscopy was used to analyse the expression of NS3, VP2 or VP7 proteins after infection with recombinant adenoviruses Ad5-BTV-NS3, Ad5-BTV-VP2 or Ad5-BTV-VP7. (A) Vero cells infected with Ad5-BTV-NS3, Ad5-BTV-VP2 or Ad5-BTV-VP7. (B) ST cells infected with Ad5-BTV-NS3, Ad5-BTV-VP2 or Ad5-BTV-VP7. The <t>three</t> panels shown from left to right are antibody staining against BTV-8 (green), DAPI staining (blue), and merge. The micrographs are representative of at least three independent experiments. Magnification 20X; bar graphs correspond to 50 nm.
    Applied Biosystems Dna Analyzer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 131 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher genomic dna
    Expression of BTV-8- <t>NS3,</t> VP2 and VP7 proteins from recombinant adenoviruses in Vero and ST cells. Fluorescence microscopy was used to analyse the expression of NS3, VP2 or VP7 proteins after infection with recombinant adenoviruses Ad5-BTV-NS3, Ad5-BTV-VP2 or Ad5-BTV-VP7. (A) Vero cells infected with Ad5-BTV-NS3, Ad5-BTV-VP2 or Ad5-BTV-VP7. (B) ST cells infected with Ad5-BTV-NS3, Ad5-BTV-VP2 or Ad5-BTV-VP7. The <t>three</t> panels shown from left to right are antibody staining against BTV-8 (green), DAPI staining (blue), and merge. The micrographs are representative of at least three independent experiments. Magnification 20X; bar graphs correspond to 50 nm.
    Genomic Dna, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 48727 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher ion torrent pgm
    Expression of BTV-8- <t>NS3,</t> VP2 and VP7 proteins from recombinant adenoviruses in Vero and ST cells. Fluorescence microscopy was used to analyse the expression of NS3, VP2 or VP7 proteins after infection with recombinant adenoviruses Ad5-BTV-NS3, Ad5-BTV-VP2 or Ad5-BTV-VP7. (A) Vero cells infected with Ad5-BTV-NS3, Ad5-BTV-VP2 or Ad5-BTV-VP7. (B) ST cells infected with Ad5-BTV-NS3, Ad5-BTV-VP2 or Ad5-BTV-VP7. The <t>three</t> panels shown from left to right are antibody staining against BTV-8 (green), DAPI staining (blue), and merge. The micrographs are representative of at least three independent experiments. Magnification 20X; bar graphs correspond to 50 nm.
    Ion Torrent Pgm, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 10392 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher random hexamer primers
    Expression of BTV-8- <t>NS3,</t> VP2 and VP7 proteins from recombinant adenoviruses in Vero and ST cells. Fluorescence microscopy was used to analyse the expression of NS3, VP2 or VP7 proteins after infection with recombinant adenoviruses Ad5-BTV-NS3, Ad5-BTV-VP2 or Ad5-BTV-VP7. (A) Vero cells infected with Ad5-BTV-NS3, Ad5-BTV-VP2 or Ad5-BTV-VP7. (B) ST cells infected with Ad5-BTV-NS3, Ad5-BTV-VP2 or Ad5-BTV-VP7. The <t>three</t> panels shown from left to right are antibody staining against BTV-8 (green), DAPI staining (blue), and merge. The micrographs are representative of at least three independent experiments. Magnification 20X; bar graphs correspond to 50 nm.
    Random Hexamer Primers, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 21036 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Meridian Life Science biomix red
    Expression of BTV-8- <t>NS3,</t> VP2 and VP7 proteins from recombinant adenoviruses in Vero and ST cells. Fluorescence microscopy was used to analyse the expression of NS3, VP2 or VP7 proteins after infection with recombinant adenoviruses Ad5-BTV-NS3, Ad5-BTV-VP2 or Ad5-BTV-VP7. (A) Vero cells infected with Ad5-BTV-NS3, Ad5-BTV-VP2 or Ad5-BTV-VP7. (B) ST cells infected with Ad5-BTV-NS3, Ad5-BTV-VP2 or Ad5-BTV-VP7. The <t>three</t> panels shown from left to right are antibody staining against BTV-8 (green), DAPI staining (blue), and merge. The micrographs are representative of at least three independent experiments. Magnification 20X; bar graphs correspond to 50 nm.
    Biomix Red, supplied by Meridian Life Science, used in various techniques. Bioz Stars score: 91/100, based on 671 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore betaine
    Characterization of DNA analogs. ( A ) PCR assays analyzed by 5% native polyacrylamide gel electrophoresis. Total PCR volume 100 µl: 20 ng 418-bp DNA template (pJ1506), 0.4 mM each LJM-3222 (5'-G TA CGC AG T ) and LJM-3223 (5'-TGTGAGT AGCTCACTCAT AG ), 0.2 mM each dNTP with indicated analog triphosphate ( 1–9 ) completely replacing appropriate dNTP, and 5 U DNA polymerase (indicated with plus symbol) with associated buffer and cycle conditions. Taq DNA polymerase ( Taq ) conditions: Taq DNA polymerase buffer with 100 mg/ml BSA and 2 mM MgCl ; 98°C (3 min), 30 cycles of [94°C (30 s), 60°C (30 s), and 72°C (45 s)], 72°C (5 min). PrimeSTAR HS DNA polymerase (PS) conditions: PrimeSTAR GC buffer with 2 M <t>betaine;</t> 98°C (3 min), 30 cycles of [98°C (15 s), 60°C (5 s), and 72°C (45 s)], 72°C (5 min). Pwo SuperYield DNA Polymerase ( Pwo ) conditions: Pwo PCR buffer with GC-rich solution and 2 M betaine; 98°C (3 min), 30 cycles of [98°C (1 min), 60°C (2 min), and 72°C (8 min)], 72°C (5 min). Lane 1 is marker (M) DNA (100 bp DNA ladder, Invitrogen) with 400 - and 500-bp bands indicated. ( B ) Anion exchange chromatography of 98-bp DNA-like polymers (pJ1923). Following equilibration in 20 mM Tris–HCl, pH 8 (buffer A), samples were eluted over 25 min at a 1 ml/min flow rate in a linear gradient from 50 to 100% buffer B (buffer A plus 1 M NaCl). Eluent absorbance at 260 nm (milli-absorbance units) was monitored with elution time (min).
    Betaine, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 3448 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Thermo Fisher pcr
    Characterization of DNA analogs. ( A ) PCR assays analyzed by 5% native polyacrylamide gel electrophoresis. Total PCR volume 100 µl: 20 ng 418-bp DNA template (pJ1506), 0.4 mM each LJM-3222 (5'-G TA CGC AG T ) and LJM-3223 (5'-TGTGAGT AGCTCACTCAT AG ), 0.2 mM each dNTP with indicated analog triphosphate ( 1–9 ) completely replacing appropriate dNTP, and 5 U DNA polymerase (indicated with plus symbol) with associated buffer and cycle conditions. Taq DNA polymerase ( Taq ) conditions: Taq DNA polymerase buffer with 100 mg/ml BSA and 2 mM MgCl ; 98°C (3 min), 30 cycles of [94°C (30 s), 60°C (30 s), and 72°C (45 s)], 72°C (5 min). PrimeSTAR HS DNA polymerase (PS) conditions: PrimeSTAR GC buffer with 2 M <t>betaine;</t> 98°C (3 min), 30 cycles of [98°C (15 s), 60°C (5 s), and 72°C (45 s)], 72°C (5 min). Pwo SuperYield DNA Polymerase ( Pwo ) conditions: Pwo PCR buffer with GC-rich solution and 2 M betaine; 98°C (3 min), 30 cycles of [98°C (1 min), 60°C (2 min), and 72°C (8 min)], 72°C (5 min). Lane 1 is marker (M) DNA (100 bp DNA ladder, Invitrogen) with 400 - and 500-bp bands indicated. ( B ) Anion exchange chromatography of 98-bp DNA-like polymers (pJ1923). Following equilibration in 20 mM Tris–HCl, pH 8 (buffer A), samples were eluted over 25 min at a 1 ml/min flow rate in a linear gradient from 50 to 100% buffer B (buffer A plus 1 M NaCl). Eluent absorbance at 260 nm (milli-absorbance units) was monitored with elution time (min).
    Pcr, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 111176 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Millipore dntpack
    Characterization of DNA analogs. ( A ) PCR assays analyzed by 5% native polyacrylamide gel electrophoresis. Total PCR volume 100 µl: 20 ng 418-bp DNA template (pJ1506), 0.4 mM each LJM-3222 (5'-G TA CGC AG T ) and LJM-3223 (5'-TGTGAGT AGCTCACTCAT AG ), 0.2 mM each dNTP with indicated analog triphosphate ( 1–9 ) completely replacing appropriate dNTP, and 5 U DNA polymerase (indicated with plus symbol) with associated buffer and cycle conditions. Taq DNA polymerase ( Taq ) conditions: Taq DNA polymerase buffer with 100 mg/ml BSA and 2 mM MgCl ; 98°C (3 min), 30 cycles of [94°C (30 s), 60°C (30 s), and 72°C (45 s)], 72°C (5 min). PrimeSTAR HS DNA polymerase (PS) conditions: PrimeSTAR GC buffer with 2 M <t>betaine;</t> 98°C (3 min), 30 cycles of [98°C (15 s), 60°C (5 s), and 72°C (45 s)], 72°C (5 min). Pwo SuperYield DNA Polymerase ( Pwo ) conditions: Pwo PCR buffer with GC-rich solution and 2 M betaine; 98°C (3 min), 30 cycles of [98°C (1 min), 60°C (2 min), and 72°C (8 min)], 72°C (5 min). Lane 1 is marker (M) DNA (100 bp DNA ladder, Invitrogen) with 400 - and 500-bp bands indicated. ( B ) Anion exchange chromatography of 98-bp DNA-like polymers (pJ1923). Following equilibration in 20 mM Tris–HCl, pH 8 (buffer A), samples were eluted over 25 min at a 1 ml/min flow rate in a linear gradient from 50 to 100% buffer B (buffer A plus 1 M NaCl). Eluent absorbance at 260 nm (milli-absorbance units) was monitored with elution time (min).
    Dntpack, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Qiagen hotstartaq dna polymerase
    Characterization of DNA analogs. ( A ) PCR assays analyzed by 5% native polyacrylamide gel electrophoresis. Total PCR volume 100 µl: 20 ng 418-bp DNA template (pJ1506), 0.4 mM each LJM-3222 (5'-G TA CGC AG T ) and LJM-3223 (5'-TGTGAGT AGCTCACTCAT AG ), 0.2 mM each dNTP with indicated analog triphosphate ( 1–9 ) completely replacing appropriate dNTP, and 5 U DNA polymerase (indicated with plus symbol) with associated buffer and cycle conditions. Taq DNA polymerase ( Taq ) conditions: Taq DNA polymerase buffer with 100 mg/ml BSA and 2 mM MgCl ; 98°C (3 min), 30 cycles of [94°C (30 s), 60°C (30 s), and 72°C (45 s)], 72°C (5 min). PrimeSTAR HS DNA polymerase (PS) conditions: PrimeSTAR GC buffer with 2 M <t>betaine;</t> 98°C (3 min), 30 cycles of [98°C (15 s), 60°C (5 s), and 72°C (45 s)], 72°C (5 min). Pwo SuperYield DNA Polymerase ( Pwo ) conditions: Pwo PCR buffer with GC-rich solution and 2 M betaine; 98°C (3 min), 30 cycles of [98°C (1 min), 60°C (2 min), and 72°C (8 min)], 72°C (5 min). Lane 1 is marker (M) DNA (100 bp DNA ladder, Invitrogen) with 400 - and 500-bp bands indicated. ( B ) Anion exchange chromatography of 98-bp DNA-like polymers (pJ1923). Following equilibration in 20 mM Tris–HCl, pH 8 (buffer A), samples were eluted over 25 min at a 1 ml/min flow rate in a linear gradient from 50 to 100% buffer B (buffer A plus 1 M NaCl). Eluent absorbance at 260 nm (milli-absorbance units) was monitored with elution time (min).
    Hotstartaq Dna Polymerase, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 6724 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Qiagen longrange pcr kit
    Characterization of DNA analogs. ( A ) PCR assays analyzed by 5% native polyacrylamide gel electrophoresis. Total PCR volume 100 µl: 20 ng 418-bp DNA template (pJ1506), 0.4 mM each LJM-3222 (5'-G TA CGC AG T ) and LJM-3223 (5'-TGTGAGT AGCTCACTCAT AG ), 0.2 mM each dNTP with indicated analog triphosphate ( 1–9 ) completely replacing appropriate dNTP, and 5 U DNA polymerase (indicated with plus symbol) with associated buffer and cycle conditions. Taq DNA polymerase ( Taq ) conditions: Taq DNA polymerase buffer with 100 mg/ml BSA and 2 mM MgCl ; 98°C (3 min), 30 cycles of [94°C (30 s), 60°C (30 s), and 72°C (45 s)], 72°C (5 min). PrimeSTAR HS DNA polymerase (PS) conditions: PrimeSTAR GC buffer with 2 M <t>betaine;</t> 98°C (3 min), 30 cycles of [98°C (15 s), 60°C (5 s), and 72°C (45 s)], 72°C (5 min). Pwo SuperYield DNA Polymerase ( Pwo ) conditions: Pwo PCR buffer with GC-rich solution and 2 M betaine; 98°C (3 min), 30 cycles of [98°C (1 min), 60°C (2 min), and 72°C (8 min)], 72°C (5 min). Lane 1 is marker (M) DNA (100 bp DNA ladder, Invitrogen) with 400 - and 500-bp bands indicated. ( B ) Anion exchange chromatography of 98-bp DNA-like polymers (pJ1923). Following equilibration in 20 mM Tris–HCl, pH 8 (buffer A), samples were eluted over 25 min at a 1 ml/min flow rate in a linear gradient from 50 to 100% buffer B (buffer A plus 1 M NaCl). Eluent absorbance at 260 nm (milli-absorbance units) was monitored with elution time (min).
    Longrange Pcr Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 693 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa one step sybr primescript rt pcr kit ii
    Characterization of DNA analogs. ( A ) PCR assays analyzed by 5% native polyacrylamide gel electrophoresis. Total PCR volume 100 µl: 20 ng 418-bp DNA template (pJ1506), 0.4 mM each LJM-3222 (5'-G TA CGC AG T ) and LJM-3223 (5'-TGTGAGT AGCTCACTCAT AG ), 0.2 mM each dNTP with indicated analog triphosphate ( 1–9 ) completely replacing appropriate dNTP, and 5 U DNA polymerase (indicated with plus symbol) with associated buffer and cycle conditions. Taq DNA polymerase ( Taq ) conditions: Taq DNA polymerase buffer with 100 mg/ml BSA and 2 mM MgCl ; 98°C (3 min), 30 cycles of [94°C (30 s), 60°C (30 s), and 72°C (45 s)], 72°C (5 min). PrimeSTAR HS DNA polymerase (PS) conditions: PrimeSTAR GC buffer with 2 M <t>betaine;</t> 98°C (3 min), 30 cycles of [98°C (15 s), 60°C (5 s), and 72°C (45 s)], 72°C (5 min). Pwo SuperYield DNA Polymerase ( Pwo ) conditions: Pwo PCR buffer with GC-rich solution and 2 M betaine; 98°C (3 min), 30 cycles of [98°C (1 min), 60°C (2 min), and 72°C (8 min)], 72°C (5 min). Lane 1 is marker (M) DNA (100 bp DNA ladder, Invitrogen) with 400 - and 500-bp bands indicated. ( B ) Anion exchange chromatography of 98-bp DNA-like polymers (pJ1923). Following equilibration in 20 mM Tris–HCl, pH 8 (buffer A), samples were eluted over 25 min at a 1 ml/min flow rate in a linear gradient from 50 to 100% buffer B (buffer A plus 1 M NaCl). Eluent absorbance at 260 nm (milli-absorbance units) was monitored with elution time (min).
    One Step Sybr Primescript Rt Pcr Kit Ii, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Thermo Fisher pcr8 gw topo
    Characterization of DNA analogs. ( A ) PCR assays analyzed by 5% native polyacrylamide gel electrophoresis. Total PCR volume 100 µl: 20 ng 418-bp DNA template (pJ1506), 0.4 mM each LJM-3222 (5'-G TA CGC AG T ) and LJM-3223 (5'-TGTGAGT AGCTCACTCAT AG ), 0.2 mM each dNTP with indicated analog triphosphate ( 1–9 ) completely replacing appropriate dNTP, and 5 U DNA polymerase (indicated with plus symbol) with associated buffer and cycle conditions. Taq DNA polymerase ( Taq ) conditions: Taq DNA polymerase buffer with 100 mg/ml BSA and 2 mM MgCl ; 98°C (3 min), 30 cycles of [94°C (30 s), 60°C (30 s), and 72°C (45 s)], 72°C (5 min). PrimeSTAR HS DNA polymerase (PS) conditions: PrimeSTAR GC buffer with 2 M <t>betaine;</t> 98°C (3 min), 30 cycles of [98°C (15 s), 60°C (5 s), and 72°C (45 s)], 72°C (5 min). Pwo SuperYield DNA Polymerase ( Pwo ) conditions: Pwo PCR buffer with GC-rich solution and 2 M betaine; 98°C (3 min), 30 cycles of [98°C (1 min), 60°C (2 min), and 72°C (8 min)], 72°C (5 min). Lane 1 is marker (M) DNA (100 bp DNA ladder, Invitrogen) with 400 - and 500-bp bands indicated. ( B ) Anion exchange chromatography of 98-bp DNA-like polymers (pJ1923). Following equilibration in 20 mM Tris–HCl, pH 8 (buffer A), samples were eluted over 25 min at a 1 ml/min flow rate in a linear gradient from 50 to 100% buffer B (buffer A plus 1 M NaCl). Eluent absorbance at 260 nm (milli-absorbance units) was monitored with elution time (min).
    Pcr8 Gw Topo, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 2420 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    pcr8 gw topo - by Bioz Stars, 2020-09
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    99
    Thermo Fisher verso cdna synthesis kit
    Characterization of DNA analogs. ( A ) PCR assays analyzed by 5% native polyacrylamide gel electrophoresis. Total PCR volume 100 µl: 20 ng 418-bp DNA template (pJ1506), 0.4 mM each LJM-3222 (5'-G TA CGC AG T ) and LJM-3223 (5'-TGTGAGT AGCTCACTCAT AG ), 0.2 mM each dNTP with indicated analog triphosphate ( 1–9 ) completely replacing appropriate dNTP, and 5 U DNA polymerase (indicated with plus symbol) with associated buffer and cycle conditions. Taq DNA polymerase ( Taq ) conditions: Taq DNA polymerase buffer with 100 mg/ml BSA and 2 mM MgCl ; 98°C (3 min), 30 cycles of [94°C (30 s), 60°C (30 s), and 72°C (45 s)], 72°C (5 min). PrimeSTAR HS DNA polymerase (PS) conditions: PrimeSTAR GC buffer with 2 M <t>betaine;</t> 98°C (3 min), 30 cycles of [98°C (15 s), 60°C (5 s), and 72°C (45 s)], 72°C (5 min). Pwo SuperYield DNA Polymerase ( Pwo ) conditions: Pwo PCR buffer with GC-rich solution and 2 M betaine; 98°C (3 min), 30 cycles of [98°C (1 min), 60°C (2 min), and 72°C (8 min)], 72°C (5 min). Lane 1 is marker (M) DNA (100 bp DNA ladder, Invitrogen) with 400 - and 500-bp bands indicated. ( B ) Anion exchange chromatography of 98-bp DNA-like polymers (pJ1923). Following equilibration in 20 mM Tris–HCl, pH 8 (buffer A), samples were eluted over 25 min at a 1 ml/min flow rate in a linear gradient from 50 to 100% buffer B (buffer A plus 1 M NaCl). Eluent absorbance at 260 nm (milli-absorbance units) was monitored with elution time (min).
    Verso Cdna Synthesis Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 5219 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Expression of BTV-8- NS3, VP2 and VP7 proteins from recombinant adenoviruses in Vero and ST cells. Fluorescence microscopy was used to analyse the expression of NS3, VP2 or VP7 proteins after infection with recombinant adenoviruses Ad5-BTV-NS3, Ad5-BTV-VP2 or Ad5-BTV-VP7. (A) Vero cells infected with Ad5-BTV-NS3, Ad5-BTV-VP2 or Ad5-BTV-VP7. (B) ST cells infected with Ad5-BTV-NS3, Ad5-BTV-VP2 or Ad5-BTV-VP7. The three panels shown from left to right are antibody staining against BTV-8 (green), DAPI staining (blue), and merge. The micrographs are representative of at least three independent experiments. Magnification 20X; bar graphs correspond to 50 nm.

    Journal: PLoS ONE

    Article Title: Protective Efficacy in Sheep of Adenovirus-Vectored Vaccines against Bluetongue Virus Is Associated with Specific T Cell Responses

    doi: 10.1371/journal.pone.0143273

    Figure Lengend Snippet: Expression of BTV-8- NS3, VP2 and VP7 proteins from recombinant adenoviruses in Vero and ST cells. Fluorescence microscopy was used to analyse the expression of NS3, VP2 or VP7 proteins after infection with recombinant adenoviruses Ad5-BTV-NS3, Ad5-BTV-VP2 or Ad5-BTV-VP7. (A) Vero cells infected with Ad5-BTV-NS3, Ad5-BTV-VP2 or Ad5-BTV-VP7. (B) ST cells infected with Ad5-BTV-NS3, Ad5-BTV-VP2 or Ad5-BTV-VP7. The three panels shown from left to right are antibody staining against BTV-8 (green), DAPI staining (blue), and merge. The micrographs are representative of at least three independent experiments. Magnification 20X; bar graphs correspond to 50 nm.

    Article Snippet: Reverse transcription of the NS3-, VP2- and VP7- mRNAs fragments was performed by two-step RT-PCR using SuperScript III reverse transcriptase (Invitrogen) and EHF DNApolymerase (Roche).

    Techniques: Expressing, Recombinant, Fluorescence, Microscopy, Infection, Staining

    Characterization of DNA analogs. ( A ) PCR assays analyzed by 5% native polyacrylamide gel electrophoresis. Total PCR volume 100 µl: 20 ng 418-bp DNA template (pJ1506), 0.4 mM each LJM-3222 (5'-G TA CGC AG T ) and LJM-3223 (5'-TGTGAGT AGCTCACTCAT AG ), 0.2 mM each dNTP with indicated analog triphosphate ( 1–9 ) completely replacing appropriate dNTP, and 5 U DNA polymerase (indicated with plus symbol) with associated buffer and cycle conditions. Taq DNA polymerase ( Taq ) conditions: Taq DNA polymerase buffer with 100 mg/ml BSA and 2 mM MgCl ; 98°C (3 min), 30 cycles of [94°C (30 s), 60°C (30 s), and 72°C (45 s)], 72°C (5 min). PrimeSTAR HS DNA polymerase (PS) conditions: PrimeSTAR GC buffer with 2 M betaine; 98°C (3 min), 30 cycles of [98°C (15 s), 60°C (5 s), and 72°C (45 s)], 72°C (5 min). Pwo SuperYield DNA Polymerase ( Pwo ) conditions: Pwo PCR buffer with GC-rich solution and 2 M betaine; 98°C (3 min), 30 cycles of [98°C (1 min), 60°C (2 min), and 72°C (8 min)], 72°C (5 min). Lane 1 is marker (M) DNA (100 bp DNA ladder, Invitrogen) with 400 - and 500-bp bands indicated. ( B ) Anion exchange chromatography of 98-bp DNA-like polymers (pJ1923). Following equilibration in 20 mM Tris–HCl, pH 8 (buffer A), samples were eluted over 25 min at a 1 ml/min flow rate in a linear gradient from 50 to 100% buffer B (buffer A plus 1 M NaCl). Eluent absorbance at 260 nm (milli-absorbance units) was monitored with elution time (min).

    Journal: Nucleic Acids Research

    Article Title: Mechanical properties of DNA-like polymers

    doi: 10.1093/nar/gkt808

    Figure Lengend Snippet: Characterization of DNA analogs. ( A ) PCR assays analyzed by 5% native polyacrylamide gel electrophoresis. Total PCR volume 100 µl: 20 ng 418-bp DNA template (pJ1506), 0.4 mM each LJM-3222 (5'-G TA CGC AG T ) and LJM-3223 (5'-TGTGAGT AGCTCACTCAT AG ), 0.2 mM each dNTP with indicated analog triphosphate ( 1–9 ) completely replacing appropriate dNTP, and 5 U DNA polymerase (indicated with plus symbol) with associated buffer and cycle conditions. Taq DNA polymerase ( Taq ) conditions: Taq DNA polymerase buffer with 100 mg/ml BSA and 2 mM MgCl ; 98°C (3 min), 30 cycles of [94°C (30 s), 60°C (30 s), and 72°C (45 s)], 72°C (5 min). PrimeSTAR HS DNA polymerase (PS) conditions: PrimeSTAR GC buffer with 2 M betaine; 98°C (3 min), 30 cycles of [98°C (15 s), 60°C (5 s), and 72°C (45 s)], 72°C (5 min). Pwo SuperYield DNA Polymerase ( Pwo ) conditions: Pwo PCR buffer with GC-rich solution and 2 M betaine; 98°C (3 min), 30 cycles of [98°C (1 min), 60°C (2 min), and 72°C (8 min)], 72°C (5 min). Lane 1 is marker (M) DNA (100 bp DNA ladder, Invitrogen) with 400 - and 500-bp bands indicated. ( B ) Anion exchange chromatography of 98-bp DNA-like polymers (pJ1923). Following equilibration in 20 mM Tris–HCl, pH 8 (buffer A), samples were eluted over 25 min at a 1 ml/min flow rate in a linear gradient from 50 to 100% buffer B (buffer A plus 1 M NaCl). Eluent absorbance at 260 nm (milli-absorbance units) was monitored with elution time (min).

    Article Snippet: For analogs 7 and 8 , PCR reactions (100 µl) included 20 ng template, 0.4 mM forward and reverse primers, Pwo PCR buffer (Roche), GC-rich solution (Roche), 0.2 mM each dNTP (dTTP completely replaced with analog), 2 M betaine (Sigma-Aldrich) and 5 U Pwo SuperYield DNA Polymerase (Roche).

    Techniques: Polymerase Chain Reaction, Polyacrylamide Gel Electrophoresis, Marker, Chromatography, Flow Cytometry