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  • 92
    ATCC gc 2 cells
    Cell apoptosis of BDE47 and 3-OH-BDE47 in GC-1 and <t>GC-2</t> cells. Cell apoptosis were determined using the Hoechst 33258 assay and flow cytometry assay. (a – b) GC-1 cells or GC-2 cells were treated with BDE47 (0–50 µM) or 3-OH-BDE47 (0–50 nM) for 24h and then subjected to Hochest 33258 assay to detect apoptotic cell using fluorescence microscope. (c – d) Cell apoptosis of GC-1 cells treated with BDE47 (0, 10, 50 µM) or 3-OH-BDE47 (0, 10, 50 nM) for 24h and then subjected to flow cytometry assay. (e – f) Cell apoptosis of GC-2 cells treated with BDE47 (0, 10, 50 µM) or 3-OH-BDE47 (0, 10, 50 nM) for 24 h and then subjected to flow cytometry assay. The data are expressed as the mean ± SD of three independent experiments from triplicate samples. * P
    Gc 2 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 31 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa advantage gc 2 pcr kit
    Cell apoptosis of BDE47 and 3-OH-BDE47 in GC-1 and <t>GC-2</t> cells. Cell apoptosis were determined using the Hoechst 33258 assay and flow cytometry assay. (a – b) GC-1 cells or GC-2 cells were treated with BDE47 (0–50 µM) or 3-OH-BDE47 (0–50 nM) for 24h and then subjected to Hochest 33258 assay to detect apoptotic cell using fluorescence microscope. (c – d) Cell apoptosis of GC-1 cells treated with BDE47 (0, 10, 50 µM) or 3-OH-BDE47 (0, 10, 50 nM) for 24h and then subjected to flow cytometry assay. (e – f) Cell apoptosis of GC-2 cells treated with BDE47 (0, 10, 50 µM) or 3-OH-BDE47 (0, 10, 50 nM) for 24 h and then subjected to flow cytometry assay. The data are expressed as the mean ± SD of three independent experiments from triplicate samples. * P
    Advantage Gc 2 Pcr Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 95/100, based on 297 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Becton Dickinson advantage gc 2 pcr kit
    Cell apoptosis of BDE47 and 3-OH-BDE47 in GC-1 and <t>GC-2</t> cells. Cell apoptosis were determined using the Hoechst 33258 assay and flow cytometry assay. (a – b) GC-1 cells or GC-2 cells were treated with BDE47 (0–50 µM) or 3-OH-BDE47 (0–50 nM) for 24h and then subjected to Hochest 33258 assay to detect apoptotic cell using fluorescence microscope. (c – d) Cell apoptosis of GC-1 cells treated with BDE47 (0, 10, 50 µM) or 3-OH-BDE47 (0, 10, 50 nM) for 24h and then subjected to flow cytometry assay. (e – f) Cell apoptosis of GC-2 cells treated with BDE47 (0, 10, 50 µM) or 3-OH-BDE47 (0, 10, 50 nM) for 24 h and then subjected to flow cytometry assay. The data are expressed as the mean ± SD of three independent experiments from triplicate samples. * P
    Advantage Gc 2 Pcr Kit, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 89/100, based on 177 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    96
    TaKaRa advantage gc 2 polymerase
    Cell apoptosis of BDE47 and 3-OH-BDE47 in GC-1 and <t>GC-2</t> cells. Cell apoptosis were determined using the Hoechst 33258 assay and flow cytometry assay. (a – b) GC-1 cells or GC-2 cells were treated with BDE47 (0–50 µM) or 3-OH-BDE47 (0–50 nM) for 24h and then subjected to Hochest 33258 assay to detect apoptotic cell using fluorescence microscope. (c – d) Cell apoptosis of GC-1 cells treated with BDE47 (0, 10, 50 µM) or 3-OH-BDE47 (0, 10, 50 nM) for 24h and then subjected to flow cytometry assay. (e – f) Cell apoptosis of GC-2 cells treated with BDE47 (0, 10, 50 µM) or 3-OH-BDE47 (0, 10, 50 nM) for 24 h and then subjected to flow cytometry assay. The data are expressed as the mean ± SD of three independent experiments from triplicate samples. * P
    Advantage Gc 2 Polymerase, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 200 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    96
    ATCC gc 2 spd
    Cell apoptosis of BDE47 and 3-OH-BDE47 in GC-1 and <t>GC-2</t> cells. Cell apoptosis were determined using the Hoechst 33258 assay and flow cytometry assay. (a – b) GC-1 cells or GC-2 cells were treated with BDE47 (0–50 µM) or 3-OH-BDE47 (0–50 nM) for 24h and then subjected to Hochest 33258 assay to detect apoptotic cell using fluorescence microscope. (c – d) Cell apoptosis of GC-1 cells treated with BDE47 (0, 10, 50 µM) or 3-OH-BDE47 (0, 10, 50 nM) for 24h and then subjected to flow cytometry assay. (e – f) Cell apoptosis of GC-2 cells treated with BDE47 (0, 10, 50 µM) or 3-OH-BDE47 (0, 10, 50 nM) for 24 h and then subjected to flow cytometry assay. The data are expressed as the mean ± SD of three independent experiments from triplicate samples. * P
    Gc 2 Spd, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Becton Dickinson advantage gc 2 polymerase
    Cell apoptosis of BDE47 and 3-OH-BDE47 in GC-1 and <t>GC-2</t> cells. Cell apoptosis were determined using the Hoechst 33258 assay and flow cytometry assay. (a – b) GC-1 cells or GC-2 cells were treated with BDE47 (0–50 µM) or 3-OH-BDE47 (0–50 nM) for 24h and then subjected to Hochest 33258 assay to detect apoptotic cell using fluorescence microscope. (c – d) Cell apoptosis of GC-1 cells treated with BDE47 (0, 10, 50 µM) or 3-OH-BDE47 (0, 10, 50 nM) for 24h and then subjected to flow cytometry assay. (e – f) Cell apoptosis of GC-2 cells treated with BDE47 (0, 10, 50 µM) or 3-OH-BDE47 (0, 10, 50 nM) for 24 h and then subjected to flow cytometry assay. The data are expressed as the mean ± SD of three independent experiments from triplicate samples. * P
    Advantage Gc 2 Polymerase, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 88/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Becton Dickinson advantage gc2 cdna polymerase mix
    Cell apoptosis of BDE47 and 3-OH-BDE47 in GC-1 and <t>GC-2</t> cells. Cell apoptosis were determined using the Hoechst 33258 assay and flow cytometry assay. (a – b) GC-1 cells or GC-2 cells were treated with BDE47 (0–50 µM) or 3-OH-BDE47 (0–50 nM) for 24h and then subjected to Hochest 33258 assay to detect apoptotic cell using fluorescence microscope. (c – d) Cell apoptosis of GC-1 cells treated with BDE47 (0, 10, 50 µM) or 3-OH-BDE47 (0, 10, 50 nM) for 24h and then subjected to flow cytometry assay. (e – f) Cell apoptosis of GC-2 cells treated with BDE47 (0, 10, 50 µM) or 3-OH-BDE47 (0, 10, 50 nM) for 24 h and then subjected to flow cytometry assay. The data are expressed as the mean ± SD of three independent experiments from triplicate samples. * P
    Advantage Gc2 Cdna Polymerase Mix, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 85/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Becton Dickinson advantage gc2 polymerase mix
    Cell apoptosis of BDE47 and 3-OH-BDE47 in GC-1 and <t>GC-2</t> cells. Cell apoptosis were determined using the Hoechst 33258 assay and flow cytometry assay. (a – b) GC-1 cells or GC-2 cells were treated with BDE47 (0–50 µM) or 3-OH-BDE47 (0–50 nM) for 24h and then subjected to Hochest 33258 assay to detect apoptotic cell using fluorescence microscope. (c – d) Cell apoptosis of GC-1 cells treated with BDE47 (0, 10, 50 µM) or 3-OH-BDE47 (0, 10, 50 nM) for 24h and then subjected to flow cytometry assay. (e – f) Cell apoptosis of GC-2 cells treated with BDE47 (0, 10, 50 µM) or 3-OH-BDE47 (0, 10, 50 nM) for 24 h and then subjected to flow cytometry assay. The data are expressed as the mean ± SD of three independent experiments from triplicate samples. * P
    Advantage Gc2 Polymerase Mix, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 88/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Burdick & Jackson b j gc2
    Cell apoptosis of BDE47 and 3-OH-BDE47 in GC-1 and <t>GC-2</t> cells. Cell apoptosis were determined using the Hoechst 33258 assay and flow cytometry assay. (a – b) GC-1 cells or GC-2 cells were treated with BDE47 (0–50 µM) or 3-OH-BDE47 (0–50 nM) for 24h and then subjected to Hochest 33258 assay to detect apoptotic cell using fluorescence microscope. (c – d) Cell apoptosis of GC-1 cells treated with BDE47 (0, 10, 50 µM) or 3-OH-BDE47 (0, 10, 50 nM) for 24h and then subjected to flow cytometry assay. (e – f) Cell apoptosis of GC-2 cells treated with BDE47 (0, 10, 50 µM) or 3-OH-BDE47 (0, 10, 50 nM) for 24 h and then subjected to flow cytometry assay. The data are expressed as the mean ± SD of three independent experiments from triplicate samples. * P
    B J Gc2, supplied by Burdick & Jackson, used in various techniques. Bioz Stars score: 85/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Cell apoptosis of BDE47 and 3-OH-BDE47 in GC-1 and GC-2 cells. Cell apoptosis were determined using the Hoechst 33258 assay and flow cytometry assay. (a – b) GC-1 cells or GC-2 cells were treated with BDE47 (0–50 µM) or 3-OH-BDE47 (0–50 nM) for 24h and then subjected to Hochest 33258 assay to detect apoptotic cell using fluorescence microscope. (c – d) Cell apoptosis of GC-1 cells treated with BDE47 (0, 10, 50 µM) or 3-OH-BDE47 (0, 10, 50 nM) for 24h and then subjected to flow cytometry assay. (e – f) Cell apoptosis of GC-2 cells treated with BDE47 (0, 10, 50 µM) or 3-OH-BDE47 (0, 10, 50 nM) for 24 h and then subjected to flow cytometry assay. The data are expressed as the mean ± SD of three independent experiments from triplicate samples. * P

    Journal: PLoS ONE

    Article Title: Cytochrome P450 3A1 Mediates 2,2?,4,4?-Tetrabromodiphenyl Ether-Induced Reduction of Spermatogenesis in Adult Rats

    doi: 10.1371/journal.pone.0066301

    Figure Lengend Snippet: Cell apoptosis of BDE47 and 3-OH-BDE47 in GC-1 and GC-2 cells. Cell apoptosis were determined using the Hoechst 33258 assay and flow cytometry assay. (a – b) GC-1 cells or GC-2 cells were treated with BDE47 (0–50 µM) or 3-OH-BDE47 (0–50 nM) for 24h and then subjected to Hochest 33258 assay to detect apoptotic cell using fluorescence microscope. (c – d) Cell apoptosis of GC-1 cells treated with BDE47 (0, 10, 50 µM) or 3-OH-BDE47 (0, 10, 50 nM) for 24h and then subjected to flow cytometry assay. (e – f) Cell apoptosis of GC-2 cells treated with BDE47 (0, 10, 50 µM) or 3-OH-BDE47 (0, 10, 50 nM) for 24 h and then subjected to flow cytometry assay. The data are expressed as the mean ± SD of three independent experiments from triplicate samples. * P

    Article Snippet: GC-1 and GC-2 cells were purchased from ATCC (Manassas, VA, USA).

    Techniques: Flow Cytometry, Cytometry, Fluorescence, Microscopy

    The role of hsa-miR-196a-5p in cell proliferation, apoptosis and cycle cycle in vitro . ( A ) The role of hsa-miR-196a-5p in cell proliferation. An MTT cell viability assay was performed at 24 h after the transfection of GC-2 cells with equal concentrations of hsa-miR-196a-5p mimics and hsa-miR-196a-5p inhibitor. ( B ) The role of hsa-miR-196a-5p in cell apoptosis. ( C ) The role of hsa-miR-196a-5p in cell cycle. For comparison, the expression levels of hsa-miR-196a-5p mimics or hsa-miR-196a-5p inhibitor transfected cells were compared with their respective negative controls (* P

    Journal: Scientific Reports

    Article Title: Common SNP in hsa-miR-196a-2 increases hsa-miR-196a-5p expression and predisposes to idiopathic male infertility in Chinese Han population

    doi: 10.1038/srep19825

    Figure Lengend Snippet: The role of hsa-miR-196a-5p in cell proliferation, apoptosis and cycle cycle in vitro . ( A ) The role of hsa-miR-196a-5p in cell proliferation. An MTT cell viability assay was performed at 24 h after the transfection of GC-2 cells with equal concentrations of hsa-miR-196a-5p mimics and hsa-miR-196a-5p inhibitor. ( B ) The role of hsa-miR-196a-5p in cell apoptosis. ( C ) The role of hsa-miR-196a-5p in cell cycle. For comparison, the expression levels of hsa-miR-196a-5p mimics or hsa-miR-196a-5p inhibitor transfected cells were compared with their respective negative controls (* P

    Article Snippet: Cell culture and transfections GC-2 cells were obtained from American Type Culture Collection (ATCC, Manassas VA, USA) maintained in high glucose DMEM with 10% fetal bovine serum (FBS), 100 U/mL penicillin and 100 μg/mL streptomycin under a humid atmosphere including 5% CO2 at 37 °C.

    Techniques: In Vitro, MTT Assay, Viability Assay, Transfection, Expressing