gateway pdonr221 vector Thermo Fisher Search Results


  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 90
    Thermo Fisher pdonr221 vector
    Schematic representation of the construction of specific deletion mutants in E. coli UTI89 . In the first step, homology regions A and B, flanking the deletion, were amplified by PCR. Primers were designed in such a way that fragments were generated with an attB -site on one side and with a restriction enzyme site and an overlap region of 25-30 bp on the other side. Overlap-PCR was subsequently carried out with the attB -containing primers, to join regions A and B. The resulting PCR fragment was used in a BP-reaction with the <t>pDONR221</t> plasmid. This gave rise to a plasmid containing the overlap-PCR fragment. After PmeI restriction of the plasmid, the chloramphenicol resistance marker cat was inserted. A final PCR-fragment was obtained using primers SeqLA1 and SeqLB, which are located on pDONR221. This fragment was electroporated in arabinose-induced E. coli strains harbouring the helper plasmid pKD46 expressing the Red recombinase.
    Pdonr221 Vector, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 2149 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pdonr221 vector/product/Thermo Fisher
    Average 90 stars, based on 2149 article reviews
    Price from $9.99 to $1999.99
    pdonr221 vector - by Bioz Stars, 2020-02
    90/100 stars
      Buy from Supplier

    85
    Thermo Fisher destination vector gateway adapted pegfp c1 pdonr221 eps8
    Schematic representation of the construction of specific deletion mutants in E. coli UTI89 . In the first step, homology regions A and B, flanking the deletion, were amplified by PCR. Primers were designed in such a way that fragments were generated with an attB -site on one side and with a restriction enzyme site and an overlap region of 25-30 bp on the other side. Overlap-PCR was subsequently carried out with the attB -containing primers, to join regions A and B. The resulting PCR fragment was used in a BP-reaction with the <t>pDONR221</t> plasmid. This gave rise to a plasmid containing the overlap-PCR fragment. After PmeI restriction of the plasmid, the chloramphenicol resistance marker cat was inserted. A final PCR-fragment was obtained using primers SeqLA1 and SeqLB, which are located on pDONR221. This fragment was electroporated in arabinose-induced E. coli strains harbouring the helper plasmid pKD46 expressing the Red recombinase.
    Destination Vector Gateway Adapted Pegfp C1 Pdonr221 Eps8, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/destination vector gateway adapted pegfp c1 pdonr221 eps8/product/Thermo Fisher
    Average 85 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    destination vector gateway adapted pegfp c1 pdonr221 eps8 - by Bioz Stars, 2020-02
    85/100 stars
      Buy from Supplier

    83
    Thermo Fisher multisite gateway pdonr221 plasmid set
    Schematic representation of the construction of specific deletion mutants in E. coli UTI89 . In the first step, homology regions A and B, flanking the deletion, were amplified by PCR. Primers were designed in such a way that fragments were generated with an attB -site on one side and with a restriction enzyme site and an overlap region of 25-30 bp on the other side. Overlap-PCR was subsequently carried out with the attB -containing primers, to join regions A and B. The resulting PCR fragment was used in a BP-reaction with the <t>pDONR221</t> plasmid. This gave rise to a plasmid containing the overlap-PCR fragment. After PmeI restriction of the plasmid, the chloramphenicol resistance marker cat was inserted. A final PCR-fragment was obtained using primers SeqLA1 and SeqLB, which are located on pDONR221. This fragment was electroporated in arabinose-induced E. coli strains harbouring the helper plasmid pKD46 expressing the Red recombinase.
    Multisite Gateway Pdonr221 Plasmid Set, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 83/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/multisite gateway pdonr221 plasmid set/product/Thermo Fisher
    Average 83 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    multisite gateway pdonr221 plasmid set - by Bioz Stars, 2020-02
    83/100 stars
      Buy from Supplier

    78
    Thermo Fisher att p containing gateway pdonr 221 entry vectors
    Schematic representation of the construction of specific deletion mutants in E. coli UTI89 . In the first step, homology regions A and B, flanking the deletion, were amplified by PCR. Primers were designed in such a way that fragments were generated with an attB -site on one side and with a restriction enzyme site and an overlap region of 25-30 bp on the other side. Overlap-PCR was subsequently carried out with the attB -containing primers, to join regions A and B. The resulting PCR fragment was used in a BP-reaction with the <t>pDONR221</t> plasmid. This gave rise to a plasmid containing the overlap-PCR fragment. After PmeI restriction of the plasmid, the chloramphenicol resistance marker cat was inserted. A final PCR-fragment was obtained using primers SeqLA1 and SeqLB, which are located on pDONR221. This fragment was electroporated in arabinose-induced E. coli strains harbouring the helper plasmid pKD46 expressing the Red recombinase.
    Att P Containing Gateway Pdonr 221 Entry Vectors, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 78/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/att p containing gateway pdonr 221 entry vectors/product/Thermo Fisher
    Average 78 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    att p containing gateway pdonr 221 entry vectors - by Bioz Stars, 2020-02
    78/100 stars
      Buy from Supplier

    78
    Thermo Fisher pdonr 221 p1 p5r gateway vector
    Schematic representation of the construction of specific deletion mutants in E. coli UTI89 . In the first step, homology regions A and B, flanking the deletion, were amplified by PCR. Primers were designed in such a way that fragments were generated with an attB -site on one side and with a restriction enzyme site and an overlap region of 25-30 bp on the other side. Overlap-PCR was subsequently carried out with the attB -containing primers, to join regions A and B. The resulting PCR fragment was used in a BP-reaction with the <t>pDONR221</t> plasmid. This gave rise to a plasmid containing the overlap-PCR fragment. After PmeI restriction of the plasmid, the chloramphenicol resistance marker cat was inserted. A final PCR-fragment was obtained using primers SeqLA1 and SeqLB, which are located on pDONR221. This fragment was electroporated in arabinose-induced E. coli strains harbouring the helper plasmid pKD46 expressing the Red recombinase.
    Pdonr 221 P1 P5r Gateway Vector, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 78/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pdonr 221 p1 p5r gateway vector/product/Thermo Fisher
    Average 78 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    pdonr 221 p1 p5r gateway vector - by Bioz Stars, 2020-02
    78/100 stars
      Buy from Supplier

    90
    Thermo Fisher pdonr221
    Schematic representation of the construction of specific deletion mutants in E. coli UTI89 . In the first step, homology regions A and B, flanking the deletion, were amplified by PCR. Primers were designed in such a way that fragments were generated with an attB -site on one side and with a restriction enzyme site and an overlap region of 25-30 bp on the other side. Overlap-PCR was subsequently carried out with the attB -containing primers, to join regions A and B. The resulting PCR fragment was used in a BP-reaction with the <t>pDONR221</t> plasmid. This gave rise to a plasmid containing the overlap-PCR fragment. After PmeI restriction of the plasmid, the chloramphenicol resistance marker cat was inserted. A final PCR-fragment was obtained using primers SeqLA1 and SeqLB, which are located on pDONR221. This fragment was electroporated in arabinose-induced E. coli strains harbouring the helper plasmid pKD46 expressing the Red recombinase.
    Pdonr221, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 4783 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pdonr221/product/Thermo Fisher
    Average 90 stars, based on 4783 article reviews
    Price from $9.99 to $1999.99
    pdonr221 - by Bioz Stars, 2020-02
    90/100 stars
      Buy from Supplier

    90
    Thermo Fisher pcr
    Schematic representation of the construction of specific deletion mutants in E. coli UTI89 . In the first step, homology regions A and B, flanking the deletion, were amplified by PCR. Primers were designed in such a way that fragments were generated with an attB -site on one side and with a restriction enzyme site and an overlap region of 25-30 bp on the other side. Overlap-PCR was subsequently carried out with the attB -containing primers, to join regions A and B. The resulting PCR fragment was used in a BP-reaction with the <t>pDONR221</t> plasmid. This gave rise to a plasmid containing the overlap-PCR fragment. After PmeI restriction of the plasmid, the chloramphenicol resistance marker cat was inserted. A final PCR-fragment was obtained using primers SeqLA1 and SeqLB, which are located on pDONR221. This fragment was electroporated in arabinose-induced E. coli strains harbouring the helper plasmid pKD46 expressing the Red recombinase.
    Pcr, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 83969 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr/product/Thermo Fisher
    Average 90 stars, based on 83969 article reviews
    Price from $9.99 to $1999.99
    pcr - by Bioz Stars, 2020-02
    90/100 stars
      Buy from Supplier

    Image Search Results


    Schematic representation of the construction of specific deletion mutants in E. coli UTI89 . In the first step, homology regions A and B, flanking the deletion, were amplified by PCR. Primers were designed in such a way that fragments were generated with an attB -site on one side and with a restriction enzyme site and an overlap region of 25-30 bp on the other side. Overlap-PCR was subsequently carried out with the attB -containing primers, to join regions A and B. The resulting PCR fragment was used in a BP-reaction with the pDONR221 plasmid. This gave rise to a plasmid containing the overlap-PCR fragment. After PmeI restriction of the plasmid, the chloramphenicol resistance marker cat was inserted. A final PCR-fragment was obtained using primers SeqLA1 and SeqLB, which are located on pDONR221. This fragment was electroporated in arabinose-induced E. coli strains harbouring the helper plasmid pKD46 expressing the Red recombinase.

    Journal: BMC Research Notes

    Article Title: Reproducible gene targeting in recalcitrant Escherichia coli isolates

    doi: 10.1186/1756-0500-4-213

    Figure Lengend Snippet: Schematic representation of the construction of specific deletion mutants in E. coli UTI89 . In the first step, homology regions A and B, flanking the deletion, were amplified by PCR. Primers were designed in such a way that fragments were generated with an attB -site on one side and with a restriction enzyme site and an overlap region of 25-30 bp on the other side. Overlap-PCR was subsequently carried out with the attB -containing primers, to join regions A and B. The resulting PCR fragment was used in a BP-reaction with the pDONR221 plasmid. This gave rise to a plasmid containing the overlap-PCR fragment. After PmeI restriction of the plasmid, the chloramphenicol resistance marker cat was inserted. A final PCR-fragment was obtained using primers SeqLA1 and SeqLB, which are located on pDONR221. This fragment was electroporated in arabinose-induced E. coli strains harbouring the helper plasmid pKD46 expressing the Red recombinase.

    Article Snippet: The attB sites are used for a site-specific BP-recombination reaction with the attP sites of the pDONR221 vector, using the Gateway® technology (Invitrogen).

    Techniques: Amplification, Polymerase Chain Reaction, Generated, Plasmid Preparation, Marker, Expressing