gateway pdonr221 vector Thermo Fisher Search Results


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  • 99
    Thermo Fisher vector pdonr221
    A flow chart showing the construction of a hairpin RNA (hpRNA)‐expressing RNA interference (RNAi) vector using pSGate1. A DNA fragment from a target gene was amplified by polymerase chain reaction (PCR) with primers containing the att B1 and att B2 sites. The PCR product was cloned into the plasmid <t>pDONR221</t> to create an entry clone by BP reaction. The insert in the entry clone then replaced the ccdB genes of pSGate1 by LR reaction, resulting in an RNAi construct expressing the hpRNA of the target gene.
    Vector Pdonr221, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1568 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/vector pdonr221/product/Thermo Fisher
    Average 99 stars, based on 1568 article reviews
    Price from $9.99 to $1999.99
    vector pdonr221 - by Bioz Stars, 2020-04
    99/100 stars
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    91
    OriGene gateway pdonr 221 vector
    A flow chart showing the construction of a hairpin RNA (hpRNA)‐expressing RNA interference (RNAi) vector using pSGate1. A DNA fragment from a target gene was amplified by polymerase chain reaction (PCR) with primers containing the att B1 and att B2 sites. The PCR product was cloned into the plasmid <t>pDONR221</t> to create an entry clone by BP reaction. The insert in the entry clone then replaced the ccdB genes of pSGate1 by LR reaction, resulting in an RNAi construct expressing the hpRNA of the target gene.
    Gateway Pdonr 221 Vector, supplied by OriGene, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gateway pdonr 221 vector/product/OriGene
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    gateway pdonr 221 vector - by Bioz Stars, 2020-04
    91/100 stars
      Buy from Supplier

    94
    Thermo Fisher destination vector gateway adapted pegfp c1 pdonr221 eps8
    A flow chart showing the construction of a hairpin RNA (hpRNA)‐expressing RNA interference (RNAi) vector using pSGate1. A DNA fragment from a target gene was amplified by polymerase chain reaction (PCR) with primers containing the att B1 and att B2 sites. The PCR product was cloned into the plasmid <t>pDONR221</t> to create an entry clone by BP reaction. The insert in the entry clone then replaced the ccdB genes of pSGate1 by LR reaction, resulting in an RNAi construct expressing the hpRNA of the target gene.
    Destination Vector Gateway Adapted Pegfp C1 Pdonr221 Eps8, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/destination vector gateway adapted pegfp c1 pdonr221 eps8/product/Thermo Fisher
    Average 94 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    destination vector gateway adapted pegfp c1 pdonr221 eps8 - by Bioz Stars, 2020-04
    94/100 stars
      Buy from Supplier

    86
    Thermo Fisher pdonr 221 p1 p5r gateway vector
    A flow chart showing the construction of a hairpin RNA (hpRNA)‐expressing RNA interference (RNAi) vector using pSGate1. A DNA fragment from a target gene was amplified by polymerase chain reaction (PCR) with primers containing the att B1 and att B2 sites. The PCR product was cloned into the plasmid <t>pDONR221</t> to create an entry clone by BP reaction. The insert in the entry clone then replaced the ccdB genes of pSGate1 by LR reaction, resulting in an RNAi construct expressing the hpRNA of the target gene.
    Pdonr 221 P1 P5r Gateway Vector, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pdonr 221 p1 p5r gateway vector/product/Thermo Fisher
    Average 86 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    pdonr 221 p1 p5r gateway vector - by Bioz Stars, 2020-04
    86/100 stars
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    99
    Thermo Fisher pcr
    A flow chart showing the construction of a hairpin RNA (hpRNA)‐expressing RNA interference (RNAi) vector using pSGate1. A DNA fragment from a target gene was amplified by polymerase chain reaction (PCR) with primers containing the att B1 and att B2 sites. The PCR product was cloned into the plasmid <t>pDONR221</t> to create an entry clone by BP reaction. The insert in the entry clone then replaced the ccdB genes of pSGate1 by LR reaction, resulting in an RNAi construct expressing the hpRNA of the target gene.
    Pcr, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 83969 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr/product/Thermo Fisher
    Average 99 stars, based on 83969 article reviews
    Price from $9.99 to $1999.99
    pcr - by Bioz Stars, 2020-04
    99/100 stars
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    Image Search Results


    A flow chart showing the construction of a hairpin RNA (hpRNA)‐expressing RNA interference (RNAi) vector using pSGate1. A DNA fragment from a target gene was amplified by polymerase chain reaction (PCR) with primers containing the att B1 and att B2 sites. The PCR product was cloned into the plasmid pDONR221 to create an entry clone by BP reaction. The insert in the entry clone then replaced the ccdB genes of pSGate1 by LR reaction, resulting in an RNAi construct expressing the hpRNA of the target gene.

    Journal: Molecular Plant Pathology

    Article Title: RNA‐mediated gene silencing in the cereal fungal pathogen Cochliobolus sativus

    doi: 10.1111/j.1364-3703.2010.00666.x

    Figure Lengend Snippet: A flow chart showing the construction of a hairpin RNA (hpRNA)‐expressing RNA interference (RNAi) vector using pSGate1. A DNA fragment from a target gene was amplified by polymerase chain reaction (PCR) with primers containing the att B1 and att B2 sites. The PCR product was cloned into the plasmid pDONR221 to create an entry clone by BP reaction. The insert in the entry clone then replaced the ccdB genes of pSGate1 by LR reaction, resulting in an RNAi construct expressing the hpRNA of the target gene.

    Article Snippet: The PCR fragment was cloned into the pDONR221 vector (Invitrogen) using the BP clonase II enzyme mix (Invitrogen) to create an entry vector pDONR221‐ GFP .

    Techniques: Flow Cytometry, Expressing, Plasmid Preparation, Amplification, Polymerase Chain Reaction, Clone Assay, Construct