Journal: Investigative ophthalmology & visual science

Article Title: Three-Dimensional Neurite Characterization of Small Incision Lenticule Extraction Derived Lenticules.

doi: 10.1167/iovs.19-27566

Figure Lengend Snippet: FIGURE 4. Interaction between lenticule neurites and SCs. Colocaliza- tion of SC marker expression and stromal neurites by confocal immunofluorescence: (A–C) GAP43 (green) and TuJ1 (red). (D–F) p75NTR (green) and TuJ1 (red).

Article Snippet: Antibodies Used in This Study Antibody Source Working Concentration 1 bIII-tubulin Covance MMS435P 0.5 lg/mL 2 GAP43 Novus NB300-143 0.5 lg/mL 3 P75NTR Millipore AB1554 0.5 lg/mL 4 Phalloidin-AlexaFluor 594 conjugate Invitrogen 0.5 lg/mL 5 AlexaFluor 488 goat anti-mouse/rabbit IgG (HþL) Jackson ImmunoRes Lab Immunostaining: 1:700 6 RedX-conjugated goat anti-mouse/rabbit IgG (HþL) Jackson ImmunoRes Lab Immunostaining: 1:700 Downloaded from iovs.arvojournals.org on 10/24/2019 placed as flat-mount.

Techniques: Marker, Expressing

Journal: Materials Today Bio

Article Title: Injectable anti-inflammatory, antioxidant supramolecular nanofiber hydrogel for peripheral nerve injury repair and neuropathic pain relief

doi: 10.1016/j.mtbio.2026.102780

Figure Lengend Snippet: Histological analysis of regenerated sciatic nerves 28 days post-PNI in rats. (A) Representative cross-sectional images: HE staining (scale bar: 20 μm), TB staining (scale bar: 20 μm), Gap43 immunohistochemistry (scale bar: 20 μm), S100β immunohistochemistry (scale bar: 20 μm). (B) TEM image representation and local magnification of myelin regeneration (scale: 2 μm). (C) Quantification of average Gap43-positive area (%) in the mid-region of regenerated nerves (n = 4). (D) Quantification of average S100β-positive area in the mid-region of regenerated tissues (n = 4). (E) Statistical analysis of myelin thickness (n = 4). (F) Representative Western blot images of total Gap43, S100β and GAPDH protein expression injured nerves. (G, H) Quantitatively analyze the expression level of S100β and Gap43 were analyzed using ImageJ software (n = 4). Data are presented as the means ± SEM. ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, ∗∗∗∗P < 0.0001.

Article Snippet: The antibodies used and their dilution ratio include: NF-κB (CST, 1:1000), P- NF-κB (CST, 1:1000), Gap43 (CST, 1:1000), S100β (Abcam, 1:1000), GAPDH (Proteintech, 1:1000).

Techniques: Staining, Immunohistochemistry, Western Blot, Expressing, Software

Journal: Translational Vision Science & Technology

Article Title: Tissue Responses and Wound Healing following Laser Scleral Microporation for Presbyopia Therapy

doi: 10.1167/tvst.9.4.6

Figure Lengend Snippet: Bar graph showing that the quantification of positively staining cells of the L + C group had significantly less CD11b and CD45-positive cells than L group at 1 month (A; * P < 0.05). The evaluation of staining intensity of tenascin-C, fibronectin, α-SMA, HSP47, and GAP43 by semiquantifying the mean gray value of the intensity for the L and L + C groups at different time points (B). * P < 0.05.

Article Snippet: The following primary antibodies were then added and incubated for 2 hours at room temperature: mouse monoclonal antibody against cellular fibronectin (MAB-1940; Millipore, Billerica, MA, USA) diluted 1∶100, tenascin-C (SC-20932; Santa Cruz Biotechnology, Dallas, TA, USA) diluted 1∶200, mouse monoclonal antibody against heat shock protein 47 (HSP47) (ADI-SPA-470; Enzo Life Sciences, Lausen, Switzerland) diluted 1:200, rabbit polyclonal antibody against CD11b (AB-133357; Abcam, Cambridge, UK) diluted 1:100, mouse monoclonal antibody against α–smooth muscle actin (α-SMA) (M0851; Dako, Glostrup, Denmark) diluted 1:100, mouse monoclonal antibody against CD45 (Ab-10558; Abcam) diluted 1:100, mouse monoclonal antibody against CD31 (Ab-199012; Abcam) diluted 1:100, mouse monoclonal antibody against CD90 (LS-B3139; LifeSpan Biosciences, CA, USA) diluted 1:100 and growth-associated protein-43 (GAP43) (NB300-143; Novus Biologicals, Littleton, CO, USA) diluted 1∶500, telomerase (MA5-16034, Thermofisher, Waltham, MA, USA) diluted 1:100, and P63 (Sc8431; Santa Cruz Biotechnology, Dallas, TA, USA) diluted 1:50.

Techniques: Staining

Journal: Translational Vision Science & Technology

Article Title: Tissue Responses and Wound Healing following Laser Scleral Microporation for Presbyopia Therapy

doi: 10.1167/tvst.9.4.6

Figure Lengend Snippet: Expression of GAP43 and CD31 for the L and L + C groups at different time points. GAP43 was significantly upregulated with comparable staining intensity for both groups, and it resolved with time (A). Expression of CD31 was also observed around the microspores in all eyes at all time points (B). Nuclei were counterstained with DAPI ( blue ). Original magnification: 100×. Scale bar : 500 µm.

Article Snippet: The following primary antibodies were then added and incubated for 2 hours at room temperature: mouse monoclonal antibody against cellular fibronectin (MAB-1940; Millipore, Billerica, MA, USA) diluted 1∶100, tenascin-C (SC-20932; Santa Cruz Biotechnology, Dallas, TA, USA) diluted 1∶200, mouse monoclonal antibody against heat shock protein 47 (HSP47) (ADI-SPA-470; Enzo Life Sciences, Lausen, Switzerland) diluted 1:200, rabbit polyclonal antibody against CD11b (AB-133357; Abcam, Cambridge, UK) diluted 1:100, mouse monoclonal antibody against α–smooth muscle actin (α-SMA) (M0851; Dako, Glostrup, Denmark) diluted 1:100, mouse monoclonal antibody against CD45 (Ab-10558; Abcam) diluted 1:100, mouse monoclonal antibody against CD31 (Ab-199012; Abcam) diluted 1:100, mouse monoclonal antibody against CD90 (LS-B3139; LifeSpan Biosciences, CA, USA) diluted 1:100 and growth-associated protein-43 (GAP43) (NB300-143; Novus Biologicals, Littleton, CO, USA) diluted 1∶500, telomerase (MA5-16034, Thermofisher, Waltham, MA, USA) diluted 1:100, and P63 (Sc8431; Santa Cruz Biotechnology, Dallas, TA, USA) diluted 1:50.

Techniques: Expressing, Staining

Journal: International heart journal

Article Title: Localisation of SCN10A gene product Na(v)1.8 and novel pain-related ion channels in human heart.

doi: 10.1536/ihj.52.146

Figure Lengend Snippet: Figure 5. Nerve and vascular markers in human heart. Human atrium im- munostained for neuropeptide Y (A), botulinum toxin receptor SV2A (B), GAP43 (C), and the vascular marker vWF (D). Scale bars = 50 μm

Article Snippet: Antibodies for Immunostaining Antibodies to Source Ref # Titre Nerve marker PGP9.5 Nerve marker neurofilaments ‘cocktail’ Sodium channel Nav1.8 Connexin43 Sodium channel Nav1.5 Sodium channel Nav1.7 Sodium channel Nav1.9 Capsaicin receptor TRPV1 Purinergic receptor P2X3 Purinergic receptor P2X2 Neuropeptide Y (CPON/NPY) Botulinum toxin receptor; Synaptic vesicle 2A (SV2A) Growth Associated Protein (GAP43) Von Willebrand Factor (vWF) Nitric oxide synthase endothelial (eNOS) Ultraclone Dakocytomation and Novocastra GlaxoSmithKline Millipore Alomone GlaxoSmithKline GlaxoSmithKline GlaxoSmithKline Neuromics Roche Chemicon/Millipore Sigma Sigma Novocastra Labs Santa Cruz RA101 M0726/NCL-PERIPH K107 MAB 3067 ASC-005 lot # AN-08 K241 K186 C22 RA10109 1/8/01 # 36886 AB9608 HPA007863 Clone 7B10 prod # G9264 NCL-vWFp sc-654 1:40000 1:500/10000 1:200-400 1:5000 1: 200 1:200-400 1:200-400 1:5000-10000 1:1000 1:1000 1:10000-20000 1:500 1:50000 1:1600 1:1000 cytes (Figure 3E, F).

Techniques: Marker