Journal:

Article Title: Screening of Thermotolerant Gluconobacter Strains for Production of 5-Keto- d -Gluconic Acid and Disruption of Flavin Adenine Dinucleotide-Containing d -Gluconate Dehydrogenase ▿

doi: 10.1128/AEM.00640-09

Figure Lengend Snippet: Bacterial strains, plasmids, and primers used in this study

Article Snippet: The oligonucleotides used in this study were purchased from FASMAC Co. Ltd. (Kanagawa, Japan) and are listed in Table . table ft1 table-wrap mode="anchored" t5 TABLE 1. caption a7 Strain, plasmid, or primer Relevant trait(s) or sequence Source or reference E. coli DH5α r − m + recA1 lacZYA φ80d lac Δ( lacZ )M15 Bethesda Research Laboratories, Inc. Gluconobacter strains THE42 Parental strain 12 THE42 gndG ::Km Km r , strain THE42 with gndG disrupted This study THF55 Parental strain 12 THF55 gndG ::Km Km r , strain THF55 with gndG disrupted This study THG42 Parental strain 12 THG42 gndG ::Km Km r , strain THG42 with gndG disrupted This study Plasmids pGEM-T Easy Cloning vector Promega pTKm Contains nonpolar Km r cassette 23 pGEM-CA-F 1.9-kb PCR product inserted into pGEM-T Easy This study pGEM-CA-F::Km Km r , used for gndG disruption, Km r cassette inserted at SmaI site This study Primers gndL-F 5′-CCGTCAACTCGCAGCCTTTCTGC-3′ This study gndL-R 5′-GCGGGCAGGATATTCACGTTGGG-3′ This study F-igL-1 5′-TAGGTGACGCCGTAATCCTG-3′ This study R-igL-2 5′-TCGGATACCATCCCTATTCCC-3′ This study F-gnDH-3 5′-TCATGGACTGGGTCAATCAG-3′ This study R-gnDH-2 5′-TGCAAGCAAAGGCCAGGATT-3′ This study Open in a separate window Bacterial strains, plasmids, and primers used in this study Bacterial strains and growth conditions.

Techniques: Plasmid Preparation, Sequencing, Clone Assay

Journal: Journal of Orthopaedic Surgery and Research

Article Title: Galuteolin suppresses proliferation and inflammation in TNF-α-induced RA-FLS cells by activating HMOX1 to regulate IKKβ/NF-κB pathway

doi: 10.1186/s13018-020-02004-x

Figure Lengend Snippet: Galuteolin suppressed cell viability and induced cell apoptosis in RA-FLS cells. a CCK-8 assay was utilized to measure RA-FLS cell proliferation in TNF-α-induced cells after different concentrations of Galu treatments. b TUNEL staining was conducted to detect cell apoptosis in RA-FLS cells in the four groups. c Western blot analyses of Bax, Bcl-2 cleaved-caspase-3, and caspase-3 protein expression in RA-FLS cells with indicated treatments. * p < 0.05 and *** p < 0.001 vs. control; # p < 0.05, ## p < 0.01, and ### p < 0.001 vs. TNF-α

Article Snippet: Galuteolin (Galu) with purity of 99.7% was purchased from Chengdu Herbpurify Co., Ltd. (Chengdu, China).

Techniques: CCK-8 Assay, TUNEL Assay, Staining, Western Blot, Expressing, Control

Journal: Journal of Orthopaedic Surgery and Research

Article Title: Galuteolin suppresses proliferation and inflammation in TNF-α-induced RA-FLS cells by activating HMOX1 to regulate IKKβ/NF-κB pathway

doi: 10.1186/s13018-020-02004-x

Figure Lengend Snippet: Galuteolin inhibited pro-inflammatory cytokines in RA-FLS cells. a The mRNA expression of IL-1β, IL-6, IL-8, and MMP-1 in RA-FLS cells with indicated treatments was measured by RT-qPCR. b ELISA was used to test the levels of IL-1β, IL-6, IL-8, and MMP-1 in RA-FLS cells with indicated treatments. * p < 0.05 and *** p < 0.001 vs. control; # p < 0.05, ## p < 0.01, and ### p < 0.001 vs. TNF-α

Article Snippet: Galuteolin (Galu) with purity of 99.7% was purchased from Chengdu Herbpurify Co., Ltd. (Chengdu, China).

Techniques: Expressing, Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay, Control

Journal: Journal of Orthopaedic Surgery and Research

Article Title: Galuteolin suppresses proliferation and inflammation in TNF-α-induced RA-FLS cells by activating HMOX1 to regulate IKKβ/NF-κB pathway

doi: 10.1186/s13018-020-02004-x

Figure Lengend Snippet: Silence of HO-1 weakened the effects of galuteolin on cell viability and apoptosis of RA-FLS cells. a The HO-1 mRNA expression in RA-FLS cells with indicated treatments was measured by RT-qPCR. *** p < 0.001 vs. control; ### p < 0.001 vs. TNF-α. b At 48 h after transfection, RA-FLS cells were collected and the expression of HO-1. c Effects of HO-1 siRNA on the proliferation of RA-FLS cells at 24 h, 48 h after Galu pretreatments were evaluated using CCK-8 assay. d TUNEL staining assay was performed to investigate the cell apoptosis of RA-FLS cells at 48 h Galu pretreatments. e Western blot analyses of Bax, Bcl-2 cleaved-caspase-3, and caspase-3 proteins expression in RA-FLS cells with indicated treatments. * p < 0.05, ** p < 0.01, and *** p < 0.001 vs. Galu; # p < 0.05 and ## p < 0.01 vs. Galu + TNF-α

Article Snippet: Galuteolin (Galu) with purity of 99.7% was purchased from Chengdu Herbpurify Co., Ltd. (Chengdu, China).

Techniques: Expressing, Quantitative RT-PCR, Control, Transfection, CCK-8 Assay, TUNEL Assay, Staining, Western Blot

Journal: Journal of Orthopaedic Surgery and Research

Article Title: Galuteolin suppresses proliferation and inflammation in TNF-α-induced RA-FLS cells by activating HMOX1 to regulate IKKβ/NF-κB pathway

doi: 10.1186/s13018-020-02004-x

Figure Lengend Snippet: Silence of HO-1 weakened the effects of galuteolin on inflammation response of RA-FLS cells. a The mRNA expression of IL-1β, IL-6, IL-8, and MMP-1 was detected after indicated treatments and HO-1 siRNA transfection in RA-FLS cells by RT-qPCR. b The levels expression of IL-1β, IL-6, IL-8, and MMP-1 were detected after indicated treatments and HO-1 siRNA transfection in RA-FLS cells by ELISA. * p < 0.05 vs. Galu; # p < 0.05 and ## p < 0.01 and ### p < 0.001 vs. Galu + TNF-α

Article Snippet: Galuteolin (Galu) with purity of 99.7% was purchased from Chengdu Herbpurify Co., Ltd. (Chengdu, China).

Techniques: Expressing, Transfection, Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay

Journal: Journal of Orthopaedic Surgery and Research

Article Title: Galuteolin suppresses proliferation and inflammation in TNF-α-induced RA-FLS cells by activating HMOX1 to regulate IKKβ/NF-κB pathway

doi: 10.1186/s13018-020-02004-x

Figure Lengend Snippet: Silence of HO-1 weakened the effects of galuteolin on the IKKβ/NF-κB signaling pathway in RA-FLS cells. Western blot analyses of the IKKβ/NF-κB pathway-related proteins in RA-FLS cells with indicated treatments among six groups. * p < 0.05, ** p < 0.01, and *** p < 0.001 vs. Galu; # p < 0.05 and ### p < 0.001 vs. Galu + TNF-α

Article Snippet: Galuteolin (Galu) with purity of 99.7% was purchased from Chengdu Herbpurify Co., Ltd. (Chengdu, China).

Techniques: Western Blot