Journal: Nanomaterials

Article Title: Hybrid Platinum(IV)-Naproxen Nanostructured Drugs Reprogram Melanoma Cells and Overpower Cisplatin

doi: 10.3390/nano15171320

Figure Lengend Snippet: [CP(Npx) 2 ] and SBA-15|[CP(Npx) 2 ] induce senescence in B16 mouse melanoma cells. The cells were treated with the IC 50 of [CP(Npx) 2 ] and the MC 50 dose of SBA-15|[CP(Npx) 2 ] for 72 h and light microscopy of hematoxylin-eosin-stained B16 cells ( A ), and flow cytometric analysis of β-galactosidase activity after fluorescein di-β-D-galactopyranoside (FDG) staining ( B ) and light microscopy of Oil red O-stained B16 ( C ) were completed. Results show the mean values ± SD of three independent experiments, while the histograms and micrographs are from a representative one.

Article Snippet: Fluorescein di-β-D-galactopyranoside (FDG), anti-Notch1 and anti-beta actin antibodies were acquired from Abcam (Cambridge, UK), while β-catenin and Oct-3/4 antibodies were obtained from Santa Cruz Biotechnology (Dallas, TX, USA).

Techniques: Light Microscopy, Staining, Activity Assay