Journal: bioRxiv

Article Title: The multifaceted roles of R2R3 transcription factor Hl MYB7 in the regulation of flavonoid and bitter acids pathways, development and biotic stress in hop ( Humulus lupulus L.)

doi: 10.1101/2022.10.03.510644

Figure Lengend Snippet: Subcellular localization and Yeast two-hybrid assay of HlMYB7 transcription factor. ( A ) Confocal images showing nuclear localization of HlMYB7-GFP fusion protein (middle), a 100× magnified view of a single nucleus expression HlMYB7-GFP (bottom) and nuclear and cytomembrane localization of GFP alone (top) in leaf epidermal cells of N. benthamiana . Scale bars = 10 μm. ( B ) Yeast two-hybrid (Y2H) analysis of HlMyb7 interaction network with the transcription factors HlbHLH2, HlMYB2, HlWDR1, HlMYB8, and HlWRKY1. Co-transformation of bait and prey vectors for each combination was cultured on both nonselective media (SD/-Trp/-Leu/+Ade/+His) and selective media (SD/-Trp/-Leu/-Ade/-His) and with X-α-Gal (SD/-Trp/-Leu/-His/X-α-Gal). For each interaction, the control was combined with empty prey and bait and empty bait with the prey construct.

Article Snippet: Growth of colonies was maintained at 0.3 optical density (OD) for each spotted 5 µl with decreasing concentraions of 1, 10 −1 , 10 −2 , 10 −3 on minus Ade, His (SD/-Trp/-Leu/-Ade/-His) and with sprayed 100 µl of 4 mg/ml X-α-Gal (Takara Bio, CA, USA) in dimethylformamide on plates (SD/-Trp/-Leu/-His/X-α-Gal).

Techniques: Y2H Assay, Expressing, Transformation Assay, Cell Culture, Construct