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  • 99
    Thermo Fisher sirna
    Sirna, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 93491 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Horizon Discovery cagaguugguggacaucga
    Cagaguugguggacaucga, supplied by Horizon Discovery, used in various techniques. Bioz Stars score: 86/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Horizon Discovery gene specific sirna smartpools
    Gene Specific Sirna Smartpools, supplied by Horizon Discovery, used in various techniques. Bioz Stars score: 85/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Horizon Discovery individual sirna
    Individual Sirna, supplied by Horizon Discovery, used in various techniques. Bioz Stars score: 89/100, based on 88 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Horizon Discovery beclin 1
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    Horizon Discovery gene specific sirna pools
    Loss of <t>αSNAP</t> triggers detachment of human intestinal epithelial cells. Control SK-CO15 cells ( SK-neo ) and cells stably expressing <t>siRNA-resistant</t> bovine αSNAP ( SK -α SNAP ) were transfected with either control or two human αSNAP-specific siRNA duplexes ( D1 and D2 ). A, expression of αSNAP and its binding partner NSF was determined 72 h post-transfection. B and C , cell detachment was examined by counting adhered and total number of cells using bright field microscopy 72 h post-transfection. Data are presented as the mean ± S.E. ( n = 3); *, p
    Gene Specific Sirna Pools, supplied by Horizon Discovery, used in various techniques. Bioz Stars score: 85/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Horizon Discovery noncoding sirna duplex 2
    Loss of <t>αSNAP</t> triggers detachment of human intestinal epithelial cells. Control SK-CO15 cells ( SK-neo ) and cells stably expressing <t>siRNA-resistant</t> bovine αSNAP ( SK -α SNAP ) were transfected with either control or two human αSNAP-specific siRNA duplexes ( D1 and D2 ). A, expression of αSNAP and its binding partner NSF was determined 72 h post-transfection. B and C , cell detachment was examined by counting adhered and total number of cells using bright field microscopy 72 h post-transfection. Data are presented as the mean ± S.E. ( n = 3); *, p
    Noncoding Sirna Duplex 2, supplied by Horizon Discovery, used in various techniques. Bioz Stars score: 86/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Horizon Discovery non coding sirna duplex 2
    Loss of <t>αSNAP</t> triggers detachment of human intestinal epithelial cells. Control SK-CO15 cells ( SK-neo ) and cells stably expressing <t>siRNA-resistant</t> bovine αSNAP ( SK -α SNAP ) were transfected with either control or two human αSNAP-specific siRNA duplexes ( D1 and D2 ). A, expression of αSNAP and its binding partner NSF was determined 72 h post-transfection. B and C , cell detachment was examined by counting adhered and total number of cells using bright field microscopy 72 h post-transfection. Data are presented as the mean ± S.E. ( n = 3); *, p
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    Thermo Fisher smartpool on targetplus human nsf
    Loss of <t>αSNAP</t> triggers detachment of human intestinal epithelial cells. Control SK-CO15 cells ( SK-neo ) and cells stably expressing <t>siRNA-resistant</t> bovine αSNAP ( SK -α SNAP ) were transfected with either control or two human αSNAP-specific siRNA duplexes ( D1 and D2 ). A, expression of αSNAP and its binding partner NSF was determined 72 h post-transfection. B and C , cell detachment was examined by counting adhered and total number of cells using bright field microscopy 72 h post-transfection. Data are presented as the mean ± S.E. ( n = 3); *, p
    Smartpool On Targetplus Human Nsf, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher silencer negative control number
    Loss of <t>αSNAP</t> triggers detachment of human intestinal epithelial cells. Control SK-CO15 cells ( SK-neo ) and cells stably expressing <t>siRNA-resistant</t> bovine αSNAP ( SK -α SNAP ) were transfected with either control or two human αSNAP-specific siRNA duplexes ( D1 and D2 ). A, expression of αSNAP and its binding partner NSF was determined 72 h post-transfection. B and C , cell detachment was examined by counting adhered and total number of cells using bright field microscopy 72 h post-transfection. Data are presented as the mean ± S.E. ( n = 3); *, p
    Silencer Negative Control Number, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Loss of αSNAP triggers detachment of human intestinal epithelial cells. Control SK-CO15 cells ( SK-neo ) and cells stably expressing siRNA-resistant bovine αSNAP ( SK -α SNAP ) were transfected with either control or two human αSNAP-specific siRNA duplexes ( D1 and D2 ). A, expression of αSNAP and its binding partner NSF was determined 72 h post-transfection. B and C , cell detachment was examined by counting adhered and total number of cells using bright field microscopy 72 h post-transfection. Data are presented as the mean ± S.E. ( n = 3); *, p

    Journal: The Journal of Biological Chemistry

    Article Title: N-Ethylmaleimide-sensitive Factor Attachment Protein α (αSNAP) Regulates Matrix Adhesion and Integrin Processing in Human Epithelial Cells *

    doi: 10.1074/jbc.M113.498691

    Figure Lengend Snippet: Loss of αSNAP triggers detachment of human intestinal epithelial cells. Control SK-CO15 cells ( SK-neo ) and cells stably expressing siRNA-resistant bovine αSNAP ( SK -α SNAP ) were transfected with either control or two human αSNAP-specific siRNA duplexes ( D1 and D2 ). A, expression of αSNAP and its binding partner NSF was determined 72 h post-transfection. B and C , cell detachment was examined by counting adhered and total number of cells using bright field microscopy 72 h post-transfection. Data are presented as the mean ± S.E. ( n = 3); *, p

    Article Snippet: Individual siRNA duplexes, GAAGGUGGCUGGUUACGCU (duplex 1) and CAGAGUUGGUGGACAUCGA (duplex 2; Dharmacon, Lafayette, CO), were used to down-regulate αSNAP expression, whereas knockdown of other targets was performed by using gene-specific siRNA pools purchased either from Dharmacon or Santa Cruz Biotechnology (Dallas, TX).

    Techniques: Stable Transfection, Expressing, Transfection, Binding Assay, Microscopy

    Effect of αSNAP depletion on ECM adhesion and integrin processing are independent from induction of apoptosis. SK-CO15 cells were transfected with either control or two αSNAP-specific siRNA duplexes. 24 h later these cells were treated for an additional 48 h with either vehicle or a pan-caspase inhibitor, Q-VAD-OPH. Cells were examined for apoptosis induction and β1 integrin maturation ( A ), β1 integrin localization ( B ), and cell attachment to ECM ( C ). Arrows indicate plasma membrane labeling of β1 integrin in control SK-CO15 cells, whereas arrowheads show intracellular localization of β1 integrin in αSNAP-depleted cells treated and untreated with pan-caspase inhibitor. Data are presented as the mean ± S.E. ( n = 3); *, p

    Journal: The Journal of Biological Chemistry

    Article Title: N-Ethylmaleimide-sensitive Factor Attachment Protein α (αSNAP) Regulates Matrix Adhesion and Integrin Processing in Human Epithelial Cells *

    doi: 10.1074/jbc.M113.498691

    Figure Lengend Snippet: Effect of αSNAP depletion on ECM adhesion and integrin processing are independent from induction of apoptosis. SK-CO15 cells were transfected with either control or two αSNAP-specific siRNA duplexes. 24 h later these cells were treated for an additional 48 h with either vehicle or a pan-caspase inhibitor, Q-VAD-OPH. Cells were examined for apoptosis induction and β1 integrin maturation ( A ), β1 integrin localization ( B ), and cell attachment to ECM ( C ). Arrows indicate plasma membrane labeling of β1 integrin in control SK-CO15 cells, whereas arrowheads show intracellular localization of β1 integrin in αSNAP-depleted cells treated and untreated with pan-caspase inhibitor. Data are presented as the mean ± S.E. ( n = 3); *, p

    Article Snippet: Individual siRNA duplexes, GAAGGUGGCUGGUUACGCU (duplex 1) and CAGAGUUGGUGGACAUCGA (duplex 2; Dharmacon, Lafayette, CO), were used to down-regulate αSNAP expression, whereas knockdown of other targets was performed by using gene-specific siRNA pools purchased either from Dharmacon or Santa Cruz Biotechnology (Dallas, TX).

    Techniques: Transfection, Cell Attachment Assay, Labeling

    Inhibitory anti- β 1 integrin antibody, but not siRNA-mediated integrin depletion, inhibits epithelial ECM adhesion and invasion. A and B , SK-CO15 cells were transfected with control, β1 integrin, β4 integrin siRNAs, or co-transfected with siRNAs against these integrins and αSNAP. Expression of adhesion proteins and cell attachment to the substrate were examined 72 h post-transfection. C–E , control ( SK-neo ) and αSNAP-overexpressing ( SK -α SNAP ) epithelial cells were preincubated with either β1 integrin-inhibitory antibody, MAB13, or the isotype-matched control antibody. Cell adhesion to collagen I ( C ) and invasion into Matrigel ( D and E ) was examined as described under “Experimental Procedures.” Data are presented as the mean ± S.E. ( n = 3); *, p

    Journal: The Journal of Biological Chemistry

    Article Title: N-Ethylmaleimide-sensitive Factor Attachment Protein α (αSNAP) Regulates Matrix Adhesion and Integrin Processing in Human Epithelial Cells *

    doi: 10.1074/jbc.M113.498691

    Figure Lengend Snippet: Inhibitory anti- β 1 integrin antibody, but not siRNA-mediated integrin depletion, inhibits epithelial ECM adhesion and invasion. A and B , SK-CO15 cells were transfected with control, β1 integrin, β4 integrin siRNAs, or co-transfected with siRNAs against these integrins and αSNAP. Expression of adhesion proteins and cell attachment to the substrate were examined 72 h post-transfection. C–E , control ( SK-neo ) and αSNAP-overexpressing ( SK -α SNAP ) epithelial cells were preincubated with either β1 integrin-inhibitory antibody, MAB13, or the isotype-matched control antibody. Cell adhesion to collagen I ( C ) and invasion into Matrigel ( D and E ) was examined as described under “Experimental Procedures.” Data are presented as the mean ± S.E. ( n = 3); *, p

    Article Snippet: Individual siRNA duplexes, GAAGGUGGCUGGUUACGCU (duplex 1) and CAGAGUUGGUGGACAUCGA (duplex 2; Dharmacon, Lafayette, CO), were used to down-regulate αSNAP expression, whereas knockdown of other targets was performed by using gene-specific siRNA pools purchased either from Dharmacon or Santa Cruz Biotechnology (Dallas, TX).

    Techniques: Transfection, Expressing, Cell Attachment Assay

    Loss of αSNAP disrupts structure of focal adhesions and affects expression and processing of key FA proteins. Control and αSNAP siRNA-transfected SK-CO15 cells were fixed 72 h post-transfection and FA were visualized by immunofluorescence labeling of vinculin and confocal microscopy ( A ). Arrows show intact vinculin-based FA structures in control cells, whereas arrowheads indicate diffuse vinculin labeling in αSNAP-depleted cells. Scale bar , 20 μm. Expression and phosphorylation of major transmembrane, scaffolding, and signaling FA proteins in control and αSNAP-depleted epithelial cells was determined by immunoblotting analysis ( B ) with densitometric quantification ( C ). Data are presented as the mean ± S.E. ( n = 3); *, p

    Journal: The Journal of Biological Chemistry

    Article Title: N-Ethylmaleimide-sensitive Factor Attachment Protein α (αSNAP) Regulates Matrix Adhesion and Integrin Processing in Human Epithelial Cells *

    doi: 10.1074/jbc.M113.498691

    Figure Lengend Snippet: Loss of αSNAP disrupts structure of focal adhesions and affects expression and processing of key FA proteins. Control and αSNAP siRNA-transfected SK-CO15 cells were fixed 72 h post-transfection and FA were visualized by immunofluorescence labeling of vinculin and confocal microscopy ( A ). Arrows show intact vinculin-based FA structures in control cells, whereas arrowheads indicate diffuse vinculin labeling in αSNAP-depleted cells. Scale bar , 20 μm. Expression and phosphorylation of major transmembrane, scaffolding, and signaling FA proteins in control and αSNAP-depleted epithelial cells was determined by immunoblotting analysis ( B ) with densitometric quantification ( C ). Data are presented as the mean ± S.E. ( n = 3); *, p

    Article Snippet: Individual siRNA duplexes, GAAGGUGGCUGGUUACGCU (duplex 1) and CAGAGUUGGUGGACAUCGA (duplex 2; Dharmacon, Lafayette, CO), were used to down-regulate αSNAP expression, whereas knockdown of other targets was performed by using gene-specific siRNA pools purchased either from Dharmacon or Santa Cruz Biotechnology (Dallas, TX).

    Techniques: Expressing, Transfection, Immunofluorescence, Labeling, Confocal Microscopy, Scaffolding

    The effects of αSNAP depletion on β 1 integrin and other FA proteins can be rescued by expression of siRNA-resistant αSNAP. SK-CO15 cells stably expressing siRNA-resistant bovine αSNAP ( SK -α SNAP ) and their empty vector controls ( SK-neo ) were transfected with either control or αSNAP-specific siRNAs. A, localization of β1 integrin was determined by immunofluorescence labeling and confocal microscopy 72 h post-transfection. B, expression and processing of different FA proteins was determined by immunoblotting. Arrows indicate plasma membrane labeling of β1 integrin in control or αSNAP-siRNA-transfected and rescued cells. Arrowheads show intracellular localization of β1 integrin in αSNAP-depleted cells without rescue. Scale bar , 20 μm.

    Journal: The Journal of Biological Chemistry

    Article Title: N-Ethylmaleimide-sensitive Factor Attachment Protein α (αSNAP) Regulates Matrix Adhesion and Integrin Processing in Human Epithelial Cells *

    doi: 10.1074/jbc.M113.498691

    Figure Lengend Snippet: The effects of αSNAP depletion on β 1 integrin and other FA proteins can be rescued by expression of siRNA-resistant αSNAP. SK-CO15 cells stably expressing siRNA-resistant bovine αSNAP ( SK -α SNAP ) and their empty vector controls ( SK-neo ) were transfected with either control or αSNAP-specific siRNAs. A, localization of β1 integrin was determined by immunofluorescence labeling and confocal microscopy 72 h post-transfection. B, expression and processing of different FA proteins was determined by immunoblotting. Arrows indicate plasma membrane labeling of β1 integrin in control or αSNAP-siRNA-transfected and rescued cells. Arrowheads show intracellular localization of β1 integrin in αSNAP-depleted cells without rescue. Scale bar , 20 μm.

    Article Snippet: Individual siRNA duplexes, GAAGGUGGCUGGUUACGCU (duplex 1) and CAGAGUUGGUGGACAUCGA (duplex 2; Dharmacon, Lafayette, CO), were used to down-regulate αSNAP expression, whereas knockdown of other targets was performed by using gene-specific siRNA pools purchased either from Dharmacon or Santa Cruz Biotechnology (Dallas, TX).

    Techniques: Expressing, Stable Transfection, Plasmid Preparation, Transfection, Immunofluorescence, Labeling, Confocal Microscopy