ga3 Search Results


  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93
    Millipore ga3
    The expression pattern of GmMFT in germinating seeds with the treatments by exogenous ABA and <t>GA3.</t> A, The germination rates of soybean seeds under 10 µM ABA or 5 µM GA3 treatment; B, Relative expression of GmMFT in soybean seeds at 12 h, 24 h after treatment with 10 µM ABA or 5 µM GA3. Three genes ( ACT11 , UKN1 and UKN2 ) in combination were used for internal control. A significant difference in comparison with the “Control” was indicated with an asterisk (P
    Ga3, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 326 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ga3/product/Millipore
    Average 93 stars, based on 326 article reviews
    Price from $9.99 to $1999.99
    ga3 - by Bioz Stars, 2020-05
    93/100 stars
      Buy from Supplier

    93
    Millipore m ga3
    The expression pattern of GmMFT in germinating seeds with the treatments by exogenous ABA and <t>GA3.</t> A, The germination rates of soybean seeds under 10 µM ABA or 5 µM GA3 treatment; B, Relative expression of GmMFT in soybean seeds at 12 h, 24 h after treatment with 10 µM ABA or 5 µM GA3. Three genes ( ACT11 , UKN1 and UKN2 ) in combination were used for internal control. A significant difference in comparison with the “Control” was indicated with an asterisk (P
    M Ga3, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m ga3/product/Millipore
    Average 93 stars, based on 20 article reviews
    Price from $9.99 to $1999.99
    m ga3 - by Bioz Stars, 2020-05
    93/100 stars
      Buy from Supplier

    88
    Carl Roth GmbH m ga3
    The expression pattern of GmMFT in germinating seeds with the treatments by exogenous ABA and <t>GA3.</t> A, The germination rates of soybean seeds under 10 µM ABA or 5 µM GA3 treatment; B, Relative expression of GmMFT in soybean seeds at 12 h, 24 h after treatment with 10 µM ABA or 5 µM GA3. Three genes ( ACT11 , UKN1 and UKN2 ) in combination were used for internal control. A significant difference in comparison with the “Control” was indicated with an asterisk (P
    M Ga3, supplied by Carl Roth GmbH, used in various techniques. Bioz Stars score: 88/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m ga3/product/Carl Roth GmbH
    Average 88 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    m ga3 - by Bioz Stars, 2020-05
    88/100 stars
      Buy from Supplier

    85
    Duchefa prepared ga3
    Effect of NO on DELLA protein accumulation. A, GFP-RGA in pRGA :: GFP-RGA roots and hypocotyls, either untreated (control [C]) or treated for 2 h with 50 μ m <t>GA3</t> or 250 μ m SNP as a source of NO, visualized by confocal microscopy. GFP-RGA
    Prepared Ga3, supplied by Duchefa, used in various techniques. Bioz Stars score: 85/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/prepared ga3/product/Duchefa
    Average 85 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    prepared ga3 - by Bioz Stars, 2020-05
    85/100 stars
      Buy from Supplier

    85
    Duchefa l 1 ga3
    Effect of NO on DELLA protein accumulation. A, GFP-RGA in pRGA :: GFP-RGA roots and hypocotyls, either untreated (control [C]) or treated for 2 h with 50 μ m <t>GA3</t> or 250 μ m SNP as a source of NO, visualized by confocal microscopy. GFP-RGA
    L 1 Ga3, supplied by Duchefa, used in various techniques. Bioz Stars score: 85/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/l 1 ga3/product/Duchefa
    Average 85 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    l 1 ga3 - by Bioz Stars, 2020-05
    85/100 stars
      Buy from Supplier

    90
    Kyowa Hakko Kirin ppm ga3
    Effect of NO on DELLA protein accumulation. A, GFP-RGA in pRGA :: GFP-RGA roots and hypocotyls, either untreated (control [C]) or treated for 2 h with 50 μ m <t>GA3</t> or 250 μ m SNP as a source of NO, visualized by confocal microscopy. GFP-RGA
    Ppm Ga3, supplied by Kyowa Hakko Kirin, used in various techniques. Bioz Stars score: 90/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ppm ga3/product/Kyowa Hakko Kirin
    Average 90 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    ppm ga3 - by Bioz Stars, 2020-05
    90/100 stars
      Buy from Supplier

    85
    Agrisera anti ga3
    Effect of NO on DELLA protein accumulation. A, GFP-RGA in pRGA :: GFP-RGA roots and hypocotyls, either untreated (control [C]) or treated for 2 h with 50 μ m <t>GA3</t> or 250 μ m SNP as a source of NO, visualized by confocal microscopy. GFP-RGA
    Anti Ga3, supplied by Agrisera, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti ga3/product/Agrisera
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti ga3 - by Bioz Stars, 2020-05
    85/100 stars
      Buy from Supplier

    92
    Bio-Synthesis Inc ga3 oxidases
    Effect of NO on DELLA protein accumulation. A, GFP-RGA in pRGA :: GFP-RGA roots and hypocotyls, either untreated (control [C]) or treated for 2 h with 50 μ m <t>GA3</t> or 250 μ m SNP as a source of NO, visualized by confocal microscopy. GFP-RGA
    Ga3 Oxidases, supplied by Bio-Synthesis Inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ga3 oxidases/product/Bio-Synthesis Inc
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ga3 oxidases - by Bioz Stars, 2020-05
    92/100 stars
      Buy from Supplier

    ga3  (Bio-Rad)
    91
    Bio-Rad ga3
    Effect of NO on DELLA protein accumulation. A, GFP-RGA in pRGA :: GFP-RGA roots and hypocotyls, either untreated (control [C]) or treated for 2 h with 50 μ m <t>GA3</t> or 250 μ m SNP as a source of NO, visualized by confocal microscopy. GFP-RGA
    Ga3, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 91/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ga3/product/Bio-Rad
    Average 91 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    ga3 - by Bioz Stars, 2020-05
    91/100 stars
      Buy from Supplier

    ga3  (Duchefa)
    91
    Duchefa ga3
    The DELLA Domain Is Required and Sufficient for the Interaction with the GID1A Receptor. (A) Schematic representation of the constructs used for the yeast two-hybrid interaction study. The positions of the DELLA (D) and the VHYNP (V) domains are indicated. (B) Immunoblots with anti-GAI (AD constructs; anti-GAI antibodies) and anti-GAL4 (DBD constructs; anti-GAL4 [DBD] RK5C1; Santa Cruz Biotechnology) antibodies demonstrating the expression of the respective fusion proteins in yeast. The asterisk indicates a cross-reacting band. The expression levels of the AD:D and AD:D+V constructs in yeast could not be examined because the anti-GAI peptide antibody does not recognize the GAI N terminus. (C) Result of the yeast two-hybrid interaction study demonstrating that the DELLA domain is required and sufficient for the GA-dependent GID1A interactions. The first and third columns show yeast transformants (growth control); the second and fourth columns show the result of a qualitative LacZ filter lift assay conducted with yeast grown for 2 d in the absence (left) and presence (right) of 100 μM <t>GA3</t> (Duchefa). Indicated on the right are the fold inductions of LacZ enzyme activity of DBD:GID1 AD:construct combinations grown on GA3 over untreated samples as determined using the Galacton-Star luminescent reagent. In agreement with the qualitative assays, the DBD AD:construct combinations (negative controls) did not result in differential LacZ activity in the absence and presence of GA (data not shown).
    Ga3, supplied by Duchefa, used in various techniques. Bioz Stars score: 91/100, based on 44 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ga3/product/Duchefa
    Average 91 stars, based on 44 article reviews
    Price from $9.99 to $1999.99
    ga3 - by Bioz Stars, 2020-05
    91/100 stars
      Buy from Supplier

    ga3  (Unigene)
    91
    Unigene ga3
    Effect of exogenous <t>GA3</t> application of gibberellin on the relative expression of gibberellin-related candidate genes in two persimmon varieties.
    Ga3, supplied by Unigene, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ga3/product/Unigene
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ga3 - by Bioz Stars, 2020-05
    91/100 stars
      Buy from Supplier

    91
    Caisson Labs ga3
    Effect of exogenous <t>GA3</t> application of gibberellin on the relative expression of gibberellin-related candidate genes in two persimmon varieties.
    Ga3, supplied by Caisson Labs, used in various techniques. Bioz Stars score: 91/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ga3/product/Caisson Labs
    Average 91 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    ga3 - by Bioz Stars, 2020-05
    91/100 stars
      Buy from Supplier

    91
    FUJIFILM ga3
    Effect of exogenous <t>GA3</t> application of gibberellin on the relative expression of gibberellin-related candidate genes in two persimmon varieties.
    Ga3, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 91/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ga3/product/FUJIFILM
    Average 91 stars, based on 11 article reviews
    Price from $9.99 to $1999.99
    ga3 - by Bioz Stars, 2020-05
    91/100 stars
      Buy from Supplier

    92
    PhytoTechnology Laboratories ga3
    Effect of exogenous <t>GA3</t> application of gibberellin on the relative expression of gibberellin-related candidate genes in two persimmon varieties.
    Ga3, supplied by PhytoTechnology Laboratories, used in various techniques. Bioz Stars score: 92/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ga3/product/PhytoTechnology Laboratories
    Average 92 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    ga3 - by Bioz Stars, 2020-05
    92/100 stars
      Buy from Supplier

    91
    Thermo Fisher ga3
    Effect of exogenous <t>GA3</t> application of gibberellin on the relative expression of gibberellin-related candidate genes in two persimmon varieties.
    Ga3, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ga3/product/Thermo Fisher
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ga3 - by Bioz Stars, 2020-05
    91/100 stars
      Buy from Supplier

    90
    Carl Roth GmbH ga3
    Effect of exogenous <t>GA3</t> application of gibberellin on the relative expression of gibberellin-related candidate genes in two persimmon varieties.
    Ga3, supplied by Carl Roth GmbH, used in various techniques. Bioz Stars score: 90/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ga3/product/Carl Roth GmbH
    Average 90 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    ga3 - by Bioz Stars, 2020-05
    90/100 stars
      Buy from Supplier

    ga3  (RPI Inc)
    91
    RPI Inc ga3
    Effect of exogenous <t>GA3</t> application of gibberellin on the relative expression of gibberellin-related candidate genes in two persimmon varieties.
    Ga3, supplied by RPI Inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ga3/product/RPI Inc
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ga3 - by Bioz Stars, 2020-05
    91/100 stars
      Buy from Supplier

    91
    Merck KGaA ga3
    Two mature sperm (1) and one blue color immature sperm (2) in the <t>GA3</t> group (Aniline blue, 400×).
    Ga3, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 91/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ga3/product/Merck KGaA
    Average 91 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    ga3 - by Bioz Stars, 2020-05
    91/100 stars
      Buy from Supplier

    93
    Abbott Laboratories ga3
    Two mature sperm (1) and one blue color immature sperm (2) in the <t>GA3</t> group (Aniline blue, 400×).
    Ga3, supplied by Abbott Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ga3/product/Abbott Laboratories
    Average 93 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    ga3 - by Bioz Stars, 2020-05
    93/100 stars
      Buy from Supplier

    91
    Millipore 200 ppm ga3
    Two mature sperm (1) and one blue color immature sperm (2) in the <t>GA3</t> group (Aniline blue, 400×).
    200 Ppm Ga3, supplied by Millipore, used in various techniques. Bioz Stars score: 91/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/200 ppm ga3/product/Millipore
    Average 91 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    200 ppm ga3 - by Bioz Stars, 2020-05
    91/100 stars
      Buy from Supplier

    85
    PhytoTechnology Laboratories gibberellic acid ga3
    Two mature sperm (1) and one blue color immature sperm (2) in the <t>GA3</t> group (Aniline blue, 400×).
    Gibberellic Acid Ga3, supplied by PhytoTechnology Laboratories, used in various techniques. Bioz Stars score: 85/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gibberellic acid ga3/product/PhytoTechnology Laboratories
    Average 85 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    gibberellic acid ga3 - by Bioz Stars, 2020-05
    85/100 stars
      Buy from Supplier

    93
    Agrisera ga3 bsa conjugate
    Two mature sperm (1) and one blue color immature sperm (2) in the <t>GA3</t> group (Aniline blue, 400×).
    Ga3 Bsa Conjugate, supplied by Agrisera, used in various techniques. Bioz Stars score: 93/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ga3 bsa conjugate/product/Agrisera
    Average 93 stars, based on 13 article reviews
    Price from $9.99 to $1999.99
    ga3 bsa conjugate - by Bioz Stars, 2020-05
    93/100 stars
      Buy from Supplier

    85
    SRU Biosystems 384 well ga3 aldehyde
    Two mature sperm (1) and one blue color immature sperm (2) in the <t>GA3</t> group (Aniline blue, 400×).
    384 Well Ga3 Aldehyde, supplied by SRU Biosystems, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/384 well ga3 aldehyde/product/SRU Biosystems
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    384 well ga3 aldehyde - by Bioz Stars, 2020-05
    85/100 stars
      Buy from Supplier

    Image Search Results


    The expression pattern of GmMFT in germinating seeds with the treatments by exogenous ABA and GA3. A, The germination rates of soybean seeds under 10 µM ABA or 5 µM GA3 treatment; B, Relative expression of GmMFT in soybean seeds at 12 h, 24 h after treatment with 10 µM ABA or 5 µM GA3. Three genes ( ACT11 , UKN1 and UKN2 ) in combination were used for internal control. A significant difference in comparison with the “Control” was indicated with an asterisk (P

    Journal: PLoS ONE

    Article Title: Identification of a Soybean MOTHER OF FT AND TFL1 Homolog Involved in Regulation of Seed Germination

    doi: 10.1371/journal.pone.0099642

    Figure Lengend Snippet: The expression pattern of GmMFT in germinating seeds with the treatments by exogenous ABA and GA3. A, The germination rates of soybean seeds under 10 µM ABA or 5 µM GA3 treatment; B, Relative expression of GmMFT in soybean seeds at 12 h, 24 h after treatment with 10 µM ABA or 5 µM GA3. Three genes ( ACT11 , UKN1 and UKN2 ) in combination were used for internal control. A significant difference in comparison with the “Control” was indicated with an asterisk (P

    Article Snippet: The abiotic treatment was carried out at the same condition supplemented with 10 µM ABA (Sigma-Aldrich A1049) or 5 µM GA3 (Sigma-Aldrich G7646).

    Techniques: Expressing

    Effect of NO on DELLA protein accumulation. A, GFP-RGA in pRGA :: GFP-RGA roots and hypocotyls, either untreated (control [C]) or treated for 2 h with 50 μ m GA3 or 250 μ m SNP as a source of NO, visualized by confocal microscopy. GFP-RGA

    Journal: Plant Physiology

    Article Title: Nitric Oxide Regulates DELLA Content and PIF Expression to Promote Photomorphogenesis in Arabidopsis 1 Expression to Promote Photomorphogenesis in Arabidopsis 1 [W]

    doi: 10.1104/pp.111.177741

    Figure Lengend Snippet: Effect of NO on DELLA protein accumulation. A, GFP-RGA in pRGA :: GFP-RGA roots and hypocotyls, either untreated (control [C]) or treated for 2 h with 50 μ m GA3 or 250 μ m SNP as a source of NO, visualized by confocal microscopy. GFP-RGA

    Article Snippet: Freshly prepared GA3 (Duchefa) and PAC (Duchefa) were added at the indicated concentrations.

    Techniques: Confocal Microscopy

    The DELLA Domain Is Required and Sufficient for the Interaction with the GID1A Receptor. (A) Schematic representation of the constructs used for the yeast two-hybrid interaction study. The positions of the DELLA (D) and the VHYNP (V) domains are indicated. (B) Immunoblots with anti-GAI (AD constructs; anti-GAI antibodies) and anti-GAL4 (DBD constructs; anti-GAL4 [DBD] RK5C1; Santa Cruz Biotechnology) antibodies demonstrating the expression of the respective fusion proteins in yeast. The asterisk indicates a cross-reacting band. The expression levels of the AD:D and AD:D+V constructs in yeast could not be examined because the anti-GAI peptide antibody does not recognize the GAI N terminus. (C) Result of the yeast two-hybrid interaction study demonstrating that the DELLA domain is required and sufficient for the GA-dependent GID1A interactions. The first and third columns show yeast transformants (growth control); the second and fourth columns show the result of a qualitative LacZ filter lift assay conducted with yeast grown for 2 d in the absence (left) and presence (right) of 100 μM GA3 (Duchefa). Indicated on the right are the fold inductions of LacZ enzyme activity of DBD:GID1 AD:construct combinations grown on GA3 over untreated samples as determined using the Galacton-Star luminescent reagent. In agreement with the qualitative assays, the DBD AD:construct combinations (negative controls) did not result in differential LacZ activity in the absence and presence of GA (data not shown).

    Journal: The Plant Cell

    Article Title: The DELLA Domain of GA INSENSITIVE Mediates the Interaction with the GA INSENSITIVE DWARF1A Gibberellin Receptor of Arabidopsis [W]

    doi: 10.1105/tpc.107.051441

    Figure Lengend Snippet: The DELLA Domain Is Required and Sufficient for the Interaction with the GID1A Receptor. (A) Schematic representation of the constructs used for the yeast two-hybrid interaction study. The positions of the DELLA (D) and the VHYNP (V) domains are indicated. (B) Immunoblots with anti-GAI (AD constructs; anti-GAI antibodies) and anti-GAL4 (DBD constructs; anti-GAL4 [DBD] RK5C1; Santa Cruz Biotechnology) antibodies demonstrating the expression of the respective fusion proteins in yeast. The asterisk indicates a cross-reacting band. The expression levels of the AD:D and AD:D+V constructs in yeast could not be examined because the anti-GAI peptide antibody does not recognize the GAI N terminus. (C) Result of the yeast two-hybrid interaction study demonstrating that the DELLA domain is required and sufficient for the GA-dependent GID1A interactions. The first and third columns show yeast transformants (growth control); the second and fourth columns show the result of a qualitative LacZ filter lift assay conducted with yeast grown for 2 d in the absence (left) and presence (right) of 100 μM GA3 (Duchefa). Indicated on the right are the fold inductions of LacZ enzyme activity of DBD:GID1 AD:construct combinations grown on GA3 over untreated samples as determined using the Galacton-Star luminescent reagent. In agreement with the qualitative assays, the DBD AD:construct combinations (negative controls) did not result in differential LacZ activity in the absence and presence of GA (data not shown).

    Article Snippet: For each mutant, three replicate samples (biological replicates) were subjected to a mock spray treatment with water (uninduced control), and three replicate samples were subjected to a spray treatment with 100 μM GA3 (Duchefa).

    Techniques: Construct, Western Blot, Expressing, Activity Assay

    GAI and RGA Are Major Growth Repressors in gid1 Triple Mutants. (A) Abundance of RGA and GAI in the gid1 mutants. Immunoblots with specific anti-RGA and anti-GAI antibodies (see Supplemental Figure 2 online) using 45 μg of total protein extract from the wild type and gid1 mutants as indicated and 9 μg from sly1- 10 mutants. A cross-reacting band serves as loading control. (B) RGA and GAI are stabilized in gid1 triple mutants. Immunoblot with anti-RGA and anti-GAI antibodies using 45 μg of protein extract from wild-type and gid1 triple mutant plants after cycloheximide (CHX; 50 μM) and GA3 (100 μM) treatments as indicated. A cross-reacting band serves as loading control. (C) gid1 triple mutants are GA insensitive with respect to GA-regulated gene expression. Clustered gene tree of the 148 GA-regulated genes in the ga1 mutant (two columns on the left) and the gid1 triple mutants (two columns on the right) in the absence and presence of GA as indicated. Coloring of the gene tree is according to the color bar on the right. The complete list of GA-regulated genes and raw expression data are provided in Supplemental Table 1 online. (D) The GID1:GFP fusion protein coimmunoprecipitates with RGA in a GA-dependent manner. Immunoblots with anti-GID1 and anti-RGA antibodies using 45 μg of total protein extract as input control (left panel) and after coimmunoprecipitation (co-IP; right panel). (E) The rga- 24 and gai- t6 mutations suppress the gid1 triple mutant phenotype (6-week-old plants are shown). All plants are homozygous for the Columbia ER allele. At least four quadruple and quintuple mutant plants with identical phenotypes were identified. The rga- 24 gai- t6 double mutants with the Columbia ER allele segregating from the cross are indistinguishable from the Columbia wild type.

    Journal: The Plant Cell

    Article Title: The DELLA Domain of GA INSENSITIVE Mediates the Interaction with the GA INSENSITIVE DWARF1A Gibberellin Receptor of Arabidopsis [W]

    doi: 10.1105/tpc.107.051441

    Figure Lengend Snippet: GAI and RGA Are Major Growth Repressors in gid1 Triple Mutants. (A) Abundance of RGA and GAI in the gid1 mutants. Immunoblots with specific anti-RGA and anti-GAI antibodies (see Supplemental Figure 2 online) using 45 μg of total protein extract from the wild type and gid1 mutants as indicated and 9 μg from sly1- 10 mutants. A cross-reacting band serves as loading control. (B) RGA and GAI are stabilized in gid1 triple mutants. Immunoblot with anti-RGA and anti-GAI antibodies using 45 μg of protein extract from wild-type and gid1 triple mutant plants after cycloheximide (CHX; 50 μM) and GA3 (100 μM) treatments as indicated. A cross-reacting band serves as loading control. (C) gid1 triple mutants are GA insensitive with respect to GA-regulated gene expression. Clustered gene tree of the 148 GA-regulated genes in the ga1 mutant (two columns on the left) and the gid1 triple mutants (two columns on the right) in the absence and presence of GA as indicated. Coloring of the gene tree is according to the color bar on the right. The complete list of GA-regulated genes and raw expression data are provided in Supplemental Table 1 online. (D) The GID1:GFP fusion protein coimmunoprecipitates with RGA in a GA-dependent manner. Immunoblots with anti-GID1 and anti-RGA antibodies using 45 μg of total protein extract as input control (left panel) and after coimmunoprecipitation (co-IP; right panel). (E) The rga- 24 and gai- t6 mutations suppress the gid1 triple mutant phenotype (6-week-old plants are shown). All plants are homozygous for the Columbia ER allele. At least four quadruple and quintuple mutant plants with identical phenotypes were identified. The rga- 24 gai- t6 double mutants with the Columbia ER allele segregating from the cross are indistinguishable from the Columbia wild type.

    Article Snippet: For each mutant, three replicate samples (biological replicates) were subjected to a mock spray treatment with water (uninduced control), and three replicate samples were subjected to a spray treatment with 100 μM GA3 (Duchefa).

    Techniques: Western Blot, Mutagenesis, Expressing, Co-Immunoprecipitation Assay

    Effect of exogenous GA3 application of gibberellin on the relative expression of gibberellin-related candidate genes in two persimmon varieties.

    Journal: Oxidative Medicine and Cellular Longevity

    Article Title: Antioxidant and the Dwarfing Candidate Gene of “Nantongxiaofangshi” (Diospyros kaki Thunb.)

    doi: 10.1155/2019/1629845

    Figure Lengend Snippet: Effect of exogenous GA3 application of gibberellin on the relative expression of gibberellin-related candidate genes in two persimmon varieties.

    Article Snippet: Three genes, Unigene7595, Unigene18861, and Unigene34904, which are involved in KO synthesis, KAO synthesis, and GA20ox synthesis were only detected in “Dafangshi.” As illustrated in , in response to the exogenous application of GA3 , two genes, Unigene 25672, which is homologous to GA2ox, and CL4170.Contig4, which is homologous to DELLA protein, were significantly differentially expressed in the two varieties in response to GA3 .

    Techniques: Expressing

    Two mature sperm (1) and one blue color immature sperm (2) in the GA3 group (Aniline blue, 400×).

    Journal: Veterinary Research Forum : an International Quarterly Journal

    Article Title: Effect of gibberellic acid on the quality of sperm and in vitro fertilization outcome in adult male rats

    doi:

    Figure Lengend Snippet: Two mature sperm (1) and one blue color immature sperm (2) in the GA3 group (Aniline blue, 400×).

    Article Snippet: The required amount of the pure powder of GA3 (Merck, Darmstadt, Germany) has been weighed daily and then 100 mg of GA3 has been solved in 1 mL MA (Merck, Darmstadt, Germany) and normal saline has been added to reach its desired volume of 0.2 mL which was fed to each rat daily.

    Techniques:

    Sperm with healthy chromatin is seen green color (1) and sperms with orange color (2) head which have damaged chromatin in the GA3 group (AO, 400×).

    Journal: Veterinary Research Forum : an International Quarterly Journal

    Article Title: Effect of gibberellic acid on the quality of sperm and in vitro fertilization outcome in adult male rats

    doi:

    Figure Lengend Snippet: Sperm with healthy chromatin is seen green color (1) and sperms with orange color (2) head which have damaged chromatin in the GA3 group (AO, 400×).

    Article Snippet: The required amount of the pure powder of GA3 (Merck, Darmstadt, Germany) has been weighed daily and then 100 mg of GA3 has been solved in 1 mL MA (Merck, Darmstadt, Germany) and normal saline has been added to reach its desired volume of 0.2 mL which was fed to each rat daily.

    Techniques:

    A. In the control group, several embryos were seen at the blastocyst stage (1) and a lysed embryo (2) on day 5 after incubation; B. In the GA3 group, three embryos at the blastocyst stage (1), one blocked embryo in two-cell stage (2), an unfertilized oocyte (3) and lysed oocyte (4) was observed (Invert microscope, 200×).

    Journal: Veterinary Research Forum : an International Quarterly Journal

    Article Title: Effect of gibberellic acid on the quality of sperm and in vitro fertilization outcome in adult male rats

    doi:

    Figure Lengend Snippet: A. In the control group, several embryos were seen at the blastocyst stage (1) and a lysed embryo (2) on day 5 after incubation; B. In the GA3 group, three embryos at the blastocyst stage (1), one blocked embryo in two-cell stage (2), an unfertilized oocyte (3) and lysed oocyte (4) was observed (Invert microscope, 200×).

    Article Snippet: The required amount of the pure powder of GA3 (Merck, Darmstadt, Germany) has been weighed daily and then 100 mg of GA3 has been solved in 1 mL MA (Merck, Darmstadt, Germany) and normal saline has been added to reach its desired volume of 0.2 mL which was fed to each rat daily.

    Techniques: Incubation, Microscopy