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Image Search Results
Journal: STAR Protocols
Article Title: Characterizing diversity among human oral stem-like cancer cells using flow cytometry
doi: 10.1016/j.xpro.2022.101763
Figure Lengend Snippet: CD24, CD44, and ALDH expression in oral tumors (A) Gating strategy has been shown. (B) (i) Gate 1 shows all intact cells with high-FSC and SCC parameters. Cell debris with low-FSC and SCC as well as aggregates/ large cells with very high FSC and/or SCC values were excluded. B (ii, iii) Gate 2 and 3 were made to include only singlet cell population. B (iv) Live cells were included for analysis by drawing a viable gate for propidium iodide negative population. (C) Using DEAB-control and isotype control antibody, gates for lineage negative as in C (i), CD24/CD44 negative as in C (ii) and ALDH-Low as in C (iii) population is shown. (D) APC-conjugated lineage antibody cocktail mixed with CD31-APC was used to separate lineage marker-positive and CD31-positive cells. Lineage antibody-negative gate was drawn for including and analyzing lineage marker and CD31-negative tumor cells (D-i). From this gate, cells populated in respective gates are shown for CD24/CD44 expression (D-ii), ALHD-High and Low (D-iii) and CD24/CD44 expressing cells with ALDH-High phenotype (D-iv).
Article Snippet:
Techniques: Expressing, Marker
Journal: STAR Protocols
Article Title: Characterizing diversity among human oral stem-like cancer cells using flow cytometry
doi: 10.1016/j.xpro.2022.101763
Figure Lengend Snippet:
Article Snippet:
Techniques: Blocking Assay, Recombinant, Software, Cell Culture, Inverted Microscopy, Imaging