Journal:

Article Title: Hypoxia activates ATP-dependent potassium channels in inspiratory neurones of neonatal mice

doi: 10.1111/j.1469-7793.1998.755bm.x

Figure Lengend Snippet: Drugs were applied at the beginning of each recording and representative traces are shown at the times indicated. The dotted lines indicate the closed level and dashed lines indicate the open levels. A, time course of diazoxide action. Patch holding potential, 0 mV. B and C, two representative experiments of KATP channel inhibition in inside-out patches by tolbutamide (B) and glibenclamide (C) at holding potentials of -25 and -40 mV, respectively. The data shown in B and C were obtained in patches that had not been subjected to any prior treatment.

Article Snippet: TTX and K + channel blockers (tetraethylammonium (TEA), 4-aminopyridine, charybdotoxin, iberiotoxin, diazoxide, tolbutamide and glibenclamide) were purchased from Alomone Labs (Israel).

Techniques: Inhibition

Journal: Physiological Reports

Article Title: Macrophage migration inhibitory factor mediates protease‐activated receptor 4‐induced bladder pain through urothelial high mobility group box 1

doi: 10.14814/phy2.13549

Figure Lengend Snippet: Micturition after intravesical administration in WT and MIF KO mice

Article Snippet: Briefly, von Frey filaments of ascending bending force (0.008, 0.02 0.04, 0.07 g) were pressed to the lower abdominal region in trials of 10 before (baseline) and 24 h after intravesical instillation of either PAR4 scramble (H‐Tyr‐Ala‐Pro‐Gly‐Lys‐Phe‐NH 2 , PAR‐3933‐PI) or PAR4‐activating peptide (PAR4‐AP, a short synthetic peptide that stimulates PAR4 in a protease‐independent manner, H‐Ala‐Tyr‐Pro‐Gly‐Lys‐Phe‐NH 2 , PAR‐3674‐PI, Peptides International, Louisville, KY) (100 μ mol/L/150 μ L PBS) or dsHMGB1 (10 μ g/150 μ L PBS, HM‐121, HMGBiontech, Italy) to detect referred bladder pain.

Techniques:

Journal: Physiological Reports

Article Title: Macrophage migration inhibitory factor mediates protease‐activated receptor 4‐induced bladder pain through urothelial high mobility group box 1

doi: 10.14814/phy2.13549

Figure Lengend Snippet: Histology after intravesical administration in WT and MIF KO mice

Article Snippet: Briefly, von Frey filaments of ascending bending force (0.008, 0.02 0.04, 0.07 g) were pressed to the lower abdominal region in trials of 10 before (baseline) and 24 h after intravesical instillation of either PAR4 scramble (H‐Tyr‐Ala‐Pro‐Gly‐Lys‐Phe‐NH 2 , PAR‐3933‐PI) or PAR4‐activating peptide (PAR4‐AP, a short synthetic peptide that stimulates PAR4 in a protease‐independent manner, H‐Ala‐Tyr‐Pro‐Gly‐Lys‐Phe‐NH 2 , PAR‐3674‐PI, Peptides International, Louisville, KY) (100 μ mol/L/150 μ L PBS) or dsHMGB1 (10 μ g/150 μ L PBS, HM‐121, HMGBiontech, Italy) to detect referred bladder pain.

Techniques: