Journal: Cell death & disease

Article Title: Long non-coding RNA DSCR8 acts as a molecular sponge for miR-485-5p to activate Wnt/β-catenin signal pathway in hepatocellular carcinoma.

doi: 10.1038/s41419-018-0937-7

Figure Lengend Snippet: Fig. 7 DSCR8 activates Wnt/β-catenin pathway by DSCR8/miR-485-5p/FZD7 axis. Rescue experiments revealed that DSCR8 negatively regulated the miR-485-5p expression (a, b), whereas positively regulated both the mRNA (a, b) and protein (c, d) expression of FZD7 by DSCR8/miR-485-5p/ FZD7 axis. c, d Western blot results from rescue experiments showed that DSCR8 positively regulated the accumulation of cytoplasmic β-catenin and the accumulation of nuclear β-catenin, c-Myc expression, and cyclin D1 expression by DSCR8/miR-485-5p/FZD7 axis. n = three repeats with similar results, *P < 0.05, **P < 0.01, ***P < 0.001

Article Snippet: FZD7 Human cDNA ORF Clone (FZD7) and FZD7 siRNA (si-FZD7) were purchased from OriGene (OriGene Technologies,Inc., USA).

Techniques: Expressing, Western Blot

Journal: Cell death & disease

Article Title: Long non-coding RNA DSCR8 acts as a molecular sponge for miR-485-5p to activate Wnt/β-catenin signal pathway in hepatocellular carcinoma.

doi: 10.1038/s41419-018-0937-7

Figure Lengend Snippet: Fig. 9 Schematic of the proposed mechanism of DSCR8 in HCC. DSCR8 acts as a molecular sponge for miR-485-5p to regulate FZD7 expression, subsequently activating Wnt/β-catenin signal pathway to promote HCC progression

Article Snippet: FZD7 Human cDNA ORF Clone (FZD7) and FZD7 siRNA (si-FZD7) were purchased from OriGene (OriGene Technologies,Inc., USA).

Techniques: Expressing

Journal: The FASEB Journal

Article Title: Mapping of Wnt-Frizzled interactions by multiplex CRISPR targeting of receptor gene families

doi: 10.1096/fj.201700144R

Figure Lengend Snippet: Constructs used in this study

Article Snippet: FZD7 , pCMV6-XL5 FZD7 , Origene , SC122259 , .

Techniques: Construct, Luciferase

Journal: Cell death & disease

Article Title: Long non-coding RNA DSCR8 acts as a molecular sponge for miR-485-5p to activate Wnt/β-catenin signal pathway in hepatocellular carcinoma.

doi: 10.1038/s41419-018-0937-7

Figure Lengend Snippet: Fig. 7 DSCR8 activates Wnt/β-catenin pathway by DSCR8/miR-485-5p/FZD7 axis. Rescue experiments revealed that DSCR8 negatively regulated the miR-485-5p expression (a, b), whereas positively regulated both the mRNA (a, b) and protein (c, d) expression of FZD7 by DSCR8/miR-485-5p/ FZD7 axis. c, d Western blot results from rescue experiments showed that DSCR8 positively regulated the accumulation of cytoplasmic β-catenin and the accumulation of nuclear β-catenin, c-Myc expression, and cyclin D1 expression by DSCR8/miR-485-5p/FZD7 axis. n = three repeats with similar results, *P < 0.05, **P < 0.01, ***P < 0.001

Article Snippet: FZD7 Human cDNA ORF Clone (FZD7) and FZD7 siRNA (si-FZD7) were purchased from OriGene (OriGene Technologies,Inc., USA).

Techniques: Expressing, Western Blot

Journal: Cell death & disease

Article Title: Long non-coding RNA DSCR8 acts as a molecular sponge for miR-485-5p to activate Wnt/β-catenin signal pathway in hepatocellular carcinoma.

doi: 10.1038/s41419-018-0937-7

Figure Lengend Snippet: Fig. 9 Schematic of the proposed mechanism of DSCR8 in HCC. DSCR8 acts as a molecular sponge for miR-485-5p to regulate FZD7 expression, subsequently activating Wnt/β-catenin signal pathway to promote HCC progression

Article Snippet: FZD7 Human cDNA ORF Clone (FZD7) and FZD7 siRNA (si-FZD7) were purchased from OriGene (OriGene Technologies,Inc., USA).

Techniques: Expressing

Journal: Cell Death & Disease

Article Title: Long non-coding RNA DSCR8 acts as a molecular sponge for miR-485-5p to activate Wnt/β-catenin signal pathway in hepatocellular carcinoma

doi: 10.1038/s41419-018-0937-7

Figure Lengend Snippet: a Data from bioinformatics tools (microRNA.org, TargetScan, and miRDB) showed that there were putative binding sites between 3′UTR of FZD7-wt and miR-485-5p. FZD7-mut means mutation of binding sites in 3′UTR of FZD7. b Luciferase reporter gene assays revealed that miR-485-5p negatively regulated the luciferase activity of FZD7-wt-3′UTR, rather than of DSCR8-mut-3′UTR. The mRNA ( c , d ) and protein ( e , f ) expression of FZD7 was negatively regulated by miR-485-5p. And FZD7 clone or siRNAs reversed the effects of miR-485-5p mimics or inhibitors on FZD7 expression ( c – f ). e , f Western blot results revealed that the accumulation of cytoplasmic β-catenin and the accumulation of nuclear β-catenin, c-Myc expression, and cyclin D1 expression were negatively regulated by miR-485-5p, while reversed by FZD7 clone and siRNA. n = three repeats with similar results, ** P < 0.01, *** P < 0.001

Article Snippet: FZD7 Human cDNA ORF Clone (FZD7) and FZD7 siRNA (si-FZD7) were purchased from OriGene (OriGene Technologies,Inc., USA).

Techniques: Binding Assay, Mutagenesis, Luciferase, Activity Assay, Expressing, Western Blot

Journal: Cell Death & Disease

Article Title: Long non-coding RNA DSCR8 acts as a molecular sponge for miR-485-5p to activate Wnt/β-catenin signal pathway in hepatocellular carcinoma

doi: 10.1038/s41419-018-0937-7

Figure Lengend Snippet: Rescue experiments revealed that DSCR8 negatively regulated the miR-485-5p expression ( a , b ), whereas positively regulated both the mRNA ( a , b ) and protein ( c , d ) expression of FZD7 by DSCR8/miR-485-5p/FZD7 axis. c , d Western blot results from rescue experiments showed that DSCR8 positively regulated the accumulation of cytoplasmic β-catenin and the accumulation of nuclear β-catenin, c-Myc expression, and cyclin D1 expression by DSCR8/miR-485-5p/FZD7 axis. n = three repeats with similar results, * P < 0.05, ** P < 0.01, *** P < 0.001

Article Snippet: FZD7 Human cDNA ORF Clone (FZD7) and FZD7 siRNA (si-FZD7) were purchased from OriGene (OriGene Technologies,Inc., USA).

Techniques: Expressing, Western Blot

Journal: Cell Death & Disease

Article Title: Long non-coding RNA DSCR8 acts as a molecular sponge for miR-485-5p to activate Wnt/β-catenin signal pathway in hepatocellular carcinoma

doi: 10.1038/s41419-018-0937-7

Figure Lengend Snippet: DSCR8 acts as a molecular sponge for miR-485-5p to regulate FZD7 expression, subsequently activating Wnt/β-catenin signal pathway to promote HCC progression

Article Snippet: FZD7 Human cDNA ORF Clone (FZD7) and FZD7 siRNA (si-FZD7) were purchased from OriGene (OriGene Technologies,Inc., USA).

Techniques: Expressing