Journal: Frontiers in Immunology

Article Title: Shared and Unique Features Distinguishing Follicular T Helper and Regulatory Cells of Peripheral Lymph Node and Peyer’s Patches

doi: 10.3389/fimmu.2018.00714

Figure Lengend Snippet: Further genes of interest. (A,B) Microarray data for mRNA expression levels of the depicted genes. Same data source as in Figure E. (C) Gating strategy illustrating definition of memory B cells in Peyer’s Patches (PP). (D) Data summary showing frequencies of memory B cells in PP of Sostdc1 +/+ and Sostdc1 −/− mice. Each dot represents one mouse. Shown are mean ± SD. (E) Determination of fecal IgA by ELISA, samples were tested in duplicates. Mean ± SD are given for each dilution step. (F) Left panel: Frequencies of PD1 hi follicular T cells among all CD4 + cells in PP of Sostdc1 +/+ and Sostdc1 −/− mice according to the gating criteria shown in Figure A. Right panel: Frequencies of TFR cells among follicular cells of PP. Foxp3 was detected by intracellular stain as described in Section “ .” Shown are mean ± SD. One-way ANOVA followed by Tukey’s post hoc analysis was performed in (A,B) Data from three independent experiments ( n = 7) were pooled and two-tailed t -tests were done in (D–F) . N/A: not applicable, ns: not significant ( p > 0.05), * p < 0.05, ** p < 0.01, and *** p < 0.001.

Article Snippet: B6.Cg-Foxp3 (Foxp3) mice were provided by B. Malissen (Aix-Marseille Université, Marseille, France) and B6.Sostdc1 tm1(KOMP)Vlcg (Sostdc1 −/− ) mice by G. Loots (Lawrence Livermore National Laboratory, Livermore, USA).

Techniques: Microarray, Expressing, Enzyme-linked Immunosorbent Assay, Staining, Two Tailed Test