Journal: Journal of integrative neuroscience

Article Title: Fingolimod Alleviates Inflammation after Cerebral Ischemia via HMGB1/TLR4/NF-κB Signaling Pathway.

doi: 10.31083/j.jin2308142

Figure Lengend Snippet: Fig. 1. Fingolimod effectively reduces cerebral infarction volume, alleviates cerebral edema, and improves neurological function in MCAO/R rats. (A) On the first day post-MCAO/R procedure, TTC staining images were captured, wherein the infarcted regions of

Article Snippet: Fingolimod (Cat #HY-11063, MedChemExpress, Monmouth Junction, NJ, USA) was prepared in dimethylsulfoxide (DMSO, D8371, Beijing Solebao Technology Co., Ltd., Beijing, China) and diluted in a 0.9% NaCl saline solution to final concentrations (0.5, 1.0, and 2.0 mg/kg).

Techniques: Staining

Journal: Journal of integrative neuroscience

Article Title: Fingolimod Alleviates Inflammation after Cerebral Ischemia via HMGB1/TLR4/NF-κB Signaling Pathway.

doi: 10.31083/j.jin2308142

Figure Lengend Snippet: Fig. 2. Fingolimod inhibits inflammation in rats after MCAO/R. (A) Enzyme-Linked Immunosorbent Assay (ELISA) was employed to measure the serum concentrations of interleukin-1 beta (IL-1β) in rats, with data expressed as the mean ± SD and analyzed utilizing

Article Snippet: Fingolimod (Cat #HY-11063, MedChemExpress, Monmouth Junction, NJ, USA) was prepared in dimethylsulfoxide (DMSO, D8371, Beijing Solebao Technology Co., Ltd., Beijing, China) and diluted in a 0.9% NaCl saline solution to final concentrations (0.5, 1.0, and 2.0 mg/kg).

Techniques: Enzyme-linked Immunosorbent Assay

Journal: Journal of integrative neuroscience

Article Title: Fingolimod Alleviates Inflammation after Cerebral Ischemia via HMGB1/TLR4/NF-κB Signaling Pathway.

doi: 10.31083/j.jin2308142

Figure Lengend Snippet: Fig. 3. Fingolimod down-regulates the expression of HMGB1 in MCAO/R rats. (A) The expression levels of HMGB1 within the hippocampal region of these rats were measured using the technique of western blot analysis, with the assessment being conducted day 1

Article Snippet: Fingolimod (Cat #HY-11063, MedChemExpress, Monmouth Junction, NJ, USA) was prepared in dimethylsulfoxide (DMSO, D8371, Beijing Solebao Technology Co., Ltd., Beijing, China) and diluted in a 0.9% NaCl saline solution to final concentrations (0.5, 1.0, and 2.0 mg/kg).

Techniques: Expressing, Western Blot

Journal: Journal of integrative neuroscience

Article Title: Fingolimod Alleviates Inflammation after Cerebral Ischemia via HMGB1/TLR4/NF-κB Signaling Pathway.

doi: 10.31083/j.jin2308142

Figure Lengend Snippet: Fig. 4. Fingolimod inhibits TLR4 expression in MCAO/R rats. (A) The expression of TLR4 in the hippocampus of rats was detected

Article Snippet: Fingolimod (Cat #HY-11063, MedChemExpress, Monmouth Junction, NJ, USA) was prepared in dimethylsulfoxide (DMSO, D8371, Beijing Solebao Technology Co., Ltd., Beijing, China) and diluted in a 0.9% NaCl saline solution to final concentrations (0.5, 1.0, and 2.0 mg/kg).

Techniques: Expressing

Journal: Journal of integrative neuroscience

Article Title: Fingolimod Alleviates Inflammation after Cerebral Ischemia via HMGB1/TLR4/NF-κB Signaling Pathway.

doi: 10.31083/j.jin2308142

Figure Lengend Snippet: Fig. 5. Fingolimod treatment reduces NF-κBp65 expression in MCAO/R rats. (A) The expression of NF-κBp65 in the hippocampus of rats was detected using western blot analysis on day 1 after MCAO/R. (B) Quantification analysis of NF-κBp65 via western blot (Data

Article Snippet: Fingolimod (Cat #HY-11063, MedChemExpress, Monmouth Junction, NJ, USA) was prepared in dimethylsulfoxide (DMSO, D8371, Beijing Solebao Technology Co., Ltd., Beijing, China) and diluted in a 0.9% NaCl saline solution to final concentrations (0.5, 1.0, and 2.0 mg/kg).

Techniques: Expressing, Western Blot

Journal: Cell Death Discovery

Article Title: Preventing light-induced toxicity in a new mouse model of sector retinitis pigmentosa caused by Rhodopsin M39R variant

doi: 10.1038/s41420-025-02769-2

Figure Lengend Snippet: A Transcriptomic analysis by bulk RNA-seq of Rho M39R/M39R KI retinae compared to control mice. The volcano plot shows the top 35 differentially expressed genes by adjusted p value. S1P signalling pathway genes are underlined in red. fc_only = only fold change; not_sign = not significant; sig_only = only significant; sig+fc = significant plus fold change. B Rho M39R/+ KI mice were treated with FTY720 at 4 weeks of age. Schematic of the treatment in Rho M39R/+ KI mice. The animals were dark-adapted overnight and intraperitoneally injected with 10 mg/kg of FTY720 or vehicle (saline solution) 30 min before performing the light damage assay. The light damage consisted in performing an ERG every week for 4 times in total. Each time, the mice were preinjected with FTY720 or vehicle. C The ONL thickness was measured at day 21, after 4 rounds of ERG. FTY720-treated mice were compared to vehicle-treated and untreated mice. Mean ± SEM. Two-way ANOVA. Tukey’s multiple comparisons test between groups. (**** p < 0.0001, *** p < 0.001). Untreated N = 5, vehicle treated N = 7, FTY720 treated N = 6. D At the end of the light damage experiments, mice were culled, and the eyes were enucleated to perform further histological investigations. Rodents eyes were fixed in 4% PFA, incubated in 30% sucrose for 1–2 days, embedded in OCT (embedding matrix), cryosectioned and stained with DAPI. IHC of untreated, vehicle or FTY70 treated Rho M39R/+ superior retina after light damage. The cryosections were stained with rhodospin-4D2 (in magenta) and anti-GFAP (in yellow). Scale bar = 20 μm. E The % area occupied by GFAP-positive signal relative to the ONL, OPL, and INL was measured after thresholding GFAP staining in untreated and treated animals. Analysis performed in Fiji. Mean ± SEM. Two-way ANOVA. Tukey’s multiple comparisons test between groups. (** p < 0.001, *** p < 0.001). N = 3.

Article Snippet: Mice in dim red light were injected intraperitoneally with vehicle (saline solution) or FTY720 (Bio-techne, Tocris, 1 mg/ml in saline solution).

Techniques: RNA Sequencing, Control, Injection, Saline, Incubation, Staining

Journal: Cell Death Discovery

Article Title: Preventing light-induced toxicity in a new mouse model of sector retinitis pigmentosa caused by Rhodopsin M39R variant

doi: 10.1038/s41420-025-02769-2

Figure Lengend Snippet: A Rho M39R/M39R KI mice were treated with FTY720 at 4 weeks of age. Schematic of the FTY20 treatment in Rho M39R/M39R KI mice. Mice were dark-adapted and injected with FTY720 (10 mg/kg) 30 min prior to the light damage assay. The ERG was performed once to induce a faster degeneration. The OCT was performed at both day 0 and day 2, after 48 h from the single ERG. B The ONL thickness was measured at day 2, after a single ERG. FTY720-treated Rho M39R/M39R KI mice were compared to vehicle-treated and untreated mice. Mean ± SEM. Two-way ANOVA. Tukey’s multiple comparisons test between groups. (**** p < 0.0001, *** p < 0.001). Untreated N = 6, vehicle and FTY720 treated N = 5. C IHC of untreated, vehicle or FTY70 treated Rho M39R/M39R superior retina after light damage. The cryosections were stained with rhodospin-4D2 (in magenta). Scale bar = 20 μm. D The number of photoreceptors in the ONL was measured at 200–400 μm from the optic nerve in the inferior and superior retina. The analysis was performed on images of the central retina acquired with a microscope EVOS FL auto 2. The area of 10–20 nuclei per retina was measured and divided to total area of the ONL to calculate the total number of nuclei. The number of photoreceptors in 100 μm per treated/untreated animal was plotted. Mean ± SEM. Two-way ANOVA. Tukey’s multiple comparisons test between groups. (* p < 0.05, ** p < 0.01). N = 3.

Article Snippet: Mice in dim red light were injected intraperitoneally with vehicle (saline solution) or FTY720 (Bio-techne, Tocris, 1 mg/ml in saline solution).

Techniques: Injection, Staining, Microscopy

Journal: Scientific reports

Article Title: Effect of FTY720P on lipid accumulation in HEPG2 cells.

doi: 10.1038/s41598-023-46011-4

Figure Lengend Snippet: Figure 2. S1P and FTY720P induce lipid accumulation in HepG2 cells. Lipid accumulation in the presence of (a) Sphk1 inhibitor PF543; (b) blockers of all S1PRs. Values are means ± SEM. N = 4. Bars not sharing a common letter are considered significantly different from each other at P < 0.05; c) various concentrations of the S1P analog FTY720P. Values are means ± SEM; N = 3. *Significantly different from the control at P < 0.05.

Article Snippet: FTY720P (Cat # sc-205332A), rosiglitazone (Cat # sc-202795), anti-SREBP1 (Cat# sc-365513, Lot#B1821), anti-p-mTOR (Cat# sc-293132, Lot#K2717), anti-mTOR (Cat# sc-8319, Lot#K2415), anti-GAPDH (Cat# sc-47724, Lot# H0917) mouse primary antibodies as well as anti-mouse horse radish peroxidase (HRP) conjugated secondary antibodies (Cat# sc-2005, Lot# C2011) were purchased all from Santa Cruz Biotechnology, CA, USA.

Techniques: Control

Journal: Scientific reports

Article Title: Effect of FTY720P on lipid accumulation in HEPG2 cells.

doi: 10.1038/s41598-023-46011-4

Figure Lengend Snippet: Figure 3. Effect of FTY720P on lipid accumulation in the presence of (a) S1PR1 blocker W146. (b) S1PR2 blocker JTE 013. (c) S1PR4 blocker CYM50358. Values are means ± SEM; N = 5. Bars not sharing a common letter are considered significantly different from each other at P < 0.05.

Article Snippet: FTY720P (Cat # sc-205332A), rosiglitazone (Cat # sc-202795), anti-SREBP1 (Cat# sc-365513, Lot#B1821), anti-p-mTOR (Cat# sc-293132, Lot#K2717), anti-mTOR (Cat# sc-8319, Lot#K2415), anti-GAPDH (Cat# sc-47724, Lot# H0917) mouse primary antibodies as well as anti-mouse horse radish peroxidase (HRP) conjugated secondary antibodies (Cat# sc-2005, Lot# C2011) were purchased all from Santa Cruz Biotechnology, CA, USA.

Techniques:

Journal: Scientific reports

Article Title: Effect of FTY720P on lipid accumulation in HEPG2 cells.

doi: 10.1038/s41598-023-46011-4

Figure Lengend Snippet: Figure 4. S1PR3 and Gq mediate the FTY720P effect on lipid accumulation. (a) Effect of FTY720P on lipid accumulation in the presence of S1PR3 blocker CAY10444. (b) Effect of FTY720P on lipid accumulation in the presence of the Gq inhibitor YM254890. (c) Protein expression of S1PR3. The blot is representative of an experiment repeated 3 times. Values were normalized to GAPDH and reported as arbitrary densitometry units. All values are means ± SEM; N = 3. Bars not sharing a common letter are considered significantly different from each other at P < 0.05.

Article Snippet: FTY720P (Cat # sc-205332A), rosiglitazone (Cat # sc-202795), anti-SREBP1 (Cat# sc-365513, Lot#B1821), anti-p-mTOR (Cat# sc-293132, Lot#K2717), anti-mTOR (Cat# sc-8319, Lot#K2415), anti-GAPDH (Cat# sc-47724, Lot# H0917) mouse primary antibodies as well as anti-mouse horse radish peroxidase (HRP) conjugated secondary antibodies (Cat# sc-2005, Lot# C2011) were purchased all from Santa Cruz Biotechnology, CA, USA.

Techniques: Expressing

Journal: Scientific reports

Article Title: Effect of FTY720P on lipid accumulation in HEPG2 cells.

doi: 10.1038/s41598-023-46011-4

Figure Lengend Snippet: Figure 5. Effect of FTY720P on lipid accumulation in the presence of (a) the PI3K inhibitor wortmannin. (b) the mTOR inhibitor rapamycin. All values are means ± SEM; N = 4. Bars not sharing a common letter are considered significantly different from each other at P < 0.05.

Article Snippet: FTY720P (Cat # sc-205332A), rosiglitazone (Cat # sc-202795), anti-SREBP1 (Cat# sc-365513, Lot#B1821), anti-p-mTOR (Cat# sc-293132, Lot#K2717), anti-mTOR (Cat# sc-8319, Lot#K2415), anti-GAPDH (Cat# sc-47724, Lot# H0917) mouse primary antibodies as well as anti-mouse horse radish peroxidase (HRP) conjugated secondary antibodies (Cat# sc-2005, Lot# C2011) were purchased all from Santa Cruz Biotechnology, CA, USA.

Techniques:

Journal: Scientific reports

Article Title: Effect of FTY720P on lipid accumulation in HEPG2 cells.

doi: 10.1038/s41598-023-46011-4

Figure Lengend Snippet: Figure 6. Effect of FTY720P on lipid accumulation in the presence of (a) the SREBP inhibitor fatostatin. (b) The PPARγ inhibitor GW9662. Values are means ± SEM; N = 5. Bars not sharing a common letter are considered significantly different from each other at P < 0.05.

Article Snippet: FTY720P (Cat # sc-205332A), rosiglitazone (Cat # sc-202795), anti-SREBP1 (Cat# sc-365513, Lot#B1821), anti-p-mTOR (Cat# sc-293132, Lot#K2717), anti-mTOR (Cat# sc-8319, Lot#K2415), anti-GAPDH (Cat# sc-47724, Lot# H0917) mouse primary antibodies as well as anti-mouse horse radish peroxidase (HRP) conjugated secondary antibodies (Cat# sc-2005, Lot# C2011) were purchased all from Santa Cruz Biotechnology, CA, USA.

Techniques:

Journal: Scientific reports

Article Title: Effect of FTY720P on lipid accumulation in HEPG2 cells.

doi: 10.1038/s41598-023-46011-4

Figure Lengend Snippet: Figure 13. The signaling pathway activated by FTY720P.

Article Snippet: FTY720P (Cat # sc-205332A), rosiglitazone (Cat # sc-202795), anti-SREBP1 (Cat# sc-365513, Lot#B1821), anti-p-mTOR (Cat# sc-293132, Lot#K2717), anti-mTOR (Cat# sc-8319, Lot#K2415), anti-GAPDH (Cat# sc-47724, Lot# H0917) mouse primary antibodies as well as anti-mouse horse radish peroxidase (HRP) conjugated secondary antibodies (Cat# sc-2005, Lot# C2011) were purchased all from Santa Cruz Biotechnology, CA, USA.

Techniques:

Journal: Journal of Leukocyte Biology

Article Title: Rapid externalization of 27-kDa heat shock protein (HSP27) and atypical cell death in neutrophils treated with the sphingolipid analog drug FTY720

doi: 10.1189/jlb.3vma1114-522rr

Figure Lengend Snippet: Figure 5. RIP1K and HSP90 are involved in neutrophil death induced by FTY720. (A and B) Flow cytometric quantification of Annexin V+PI+, Annexin V+PI2, and HSP27+ neutrophils after preincubation or not with necrostatin-1 (20 mM) or Z-IETD-FMK (20 mM) for 30 min and treatment or not with FTY720 (10 mM) for 3 h. Data are from 7 independent experiments. *P , 0.05; **P , 0.01; ***P , 0.001. (C and D) Neutrophil lysates analyzed by Western blotting for caspase-8 cleavage or MLKL phosphorylation after cell death induc- tion by FTY720. Where indicated, Q-VD-OPh (20 mM), necrostatin-1 (20 mM), DPI (20 mM), or NSA (5 mM) was added for 30 min before stimulation. GAPDH analysis was performed as loading control. The results of 1 representative experiment out of 4 are presented. (E) Flow cytometric quantification of HSP27+ neutrophils incubated for 30 min in the presence of geldanamycin (10 mM), DMAG (20 mM), or radicicol (10 mM) before treatment with FTY720 (10 mM) for 3 h. Data are from 7 independent experiments. *P , 0.05; **P , 0.01.

Article Snippet: Cells were incubated with FTY720 and FTY720 (S)-phosphate (both from Santa Cruz Biotechnology, Santa Cruz, CA, USA) or cycloheximide (Sigma, St. Louis, MO, USA) at 3 3 10/ml for 3 h. In some cases, neutrophils were preincubated for 30 min with necrostatin-1 or Z-IETD-FMK (both from Sigma); Q-VD-OPh (R&D Systems, Minneapolis, MN, USA); NSA (Tocris, Bristol, United Kingdom); geldanamycin, 17-DMAG, and radicicol (all from InvivoGen, San Diego, CA, USA); OA (Santa Cruz Biotechnology); or DPI (Sigma).

Techniques: Western Blot, Phospho-proteomics, Control, Incubation