Journal: Journal of Assisted Reproduction and Genetics

Article Title: Regulating human oocyte maturation in vitro: a hypothesis based on oocytes retrieved from small antral follicles during ovarian tissue cryopreservation

doi: 10.1007/s10815-025-03483-9

Figure Lengend Snippet: Relative expression of selected genes in cumulus cells from COCs after IVM in the presence of different gonadotropin supplementation (upper figures a – e ) and according to maturation stage (GV, MI, and MII; lower figures f – j ). No GT: no gonadotropins; FSH10: 10 IU/L rFSH; FSH100: 100 IU/L rFSH; FSH100 + LH100: both 100 IU/L rFSH and 100 IU/L rLH. LHCGR , luteinizing hormone receptor; FSHR , follicle-stimulating hormone receptor; AMH , anti-Müllerian hormone; CYP19 A1 , aromatase cytochrome p450 family 19 subfamily a polypeptide 1; INHA , inhibin subunit alpha. * P < 0.05, ** P < 0.001, *** P < 0.0001

Article Snippet: The following TaqMan probes were used: LH receptor ( LHCGR ; #Hs00174885_m1), FSH receptor ( FSHR ; #Hs01019695_m1), anti-Mullerian hormone ( AMH ; #Hs00174915_m1), aromatase cytochrome p450 family 19 subfamily a polypeptide 1 ( CYP19 A1 , #Hs00903411_m1), inhibin subunit alfa ( INHA , #Hs00171410_m1), and glyceraldehyde 3-phosphate dehydrogenase ( GAPDH ; #Hs02786624_g1) and as the reference gene [ ].

Techniques: Expressing

Journal: Journal of Assisted Reproduction and Genetics

Article Title: Regulating human oocyte maturation in vitro: a hypothesis based on oocytes retrieved from small antral follicles during ovarian tissue cryopreservation

doi: 10.1007/s10815-025-03483-9

Figure Lengend Snippet: a LHCGR gene expression in relation to FSHR gene expression (Spearman association R = − 0.60 P < 0.001) and the concentration of GDF9 in spent medium after IVM (Spearman association R = − 0.35, P < 0.035). b Concentration of inhibin-A in spent medium after IVM in relation to FSHR and LHCGR gene expression (Spearman associations: FSHR : R = − 0.75, P < 0.001; LHCGR : R = 0.42, P < 0.002)

Article Snippet: The following TaqMan probes were used: LH receptor ( LHCGR ; #Hs00174885_m1), FSH receptor ( FSHR ; #Hs01019695_m1), anti-Mullerian hormone ( AMH ; #Hs00174915_m1), aromatase cytochrome p450 family 19 subfamily a polypeptide 1 ( CYP19 A1 , #Hs00903411_m1), inhibin subunit alfa ( INHA , #Hs00171410_m1), and glyceraldehyde 3-phosphate dehydrogenase ( GAPDH ; #Hs02786624_g1) and as the reference gene [ ].

Techniques: Gene Expression, Concentration Assay

Journal: Journal of Assisted Reproduction and Genetics

Article Title: Regulating human oocyte maturation in vitro: a hypothesis based on oocytes retrieved from small antral follicles during ovarian tissue cryopreservation

doi: 10.1007/s10815-025-03483-9

Figure Lengend Snippet: Working hypothesis for the regulation of oocyte human maturation in vitro of cumulus oocyte complexes from human small antral follicles: Relatively high FSHR expression is present on cumulus cells from human small antral follicles (28). High concentrations of FSH (> 75 IU/L) stimulate cumulus cells to cause immature oocytes to downregulate GDF9 secretion. Reduced GDF9 secretion allows cumulus cells to upregulate FSH-stimulated LHCGR expression. LH in the IVM medium (plus potentially FSH) causes augmented secretion of inhibin-A, which in turn stimulates germinal vesicle breakdown (GVBD) and oocyte maturation in combination with other yet unidentified positive signals secreted from cumulus cells by FSH and LH. FSHR expression becomes downregulated in COCs in which MII transition takes place probably caused by the high concentration of FSH in the medium, which, however, does not take place in COCs that remain in the GV stage. CC, cumulus cells; FSHR, FSH receptor; LHCGR, LH receptor; GDF9, growth and differentiation factor 9. Up and downregulation is depicted by arrows pointing up and down, respectively

Article Snippet: The following TaqMan probes were used: LH receptor ( LHCGR ; #Hs00174885_m1), FSH receptor ( FSHR ; #Hs01019695_m1), anti-Mullerian hormone ( AMH ; #Hs00174915_m1), aromatase cytochrome p450 family 19 subfamily a polypeptide 1 ( CYP19 A1 , #Hs00903411_m1), inhibin subunit alfa ( INHA , #Hs00171410_m1), and glyceraldehyde 3-phosphate dehydrogenase ( GAPDH ; #Hs02786624_g1) and as the reference gene [ ].

Techniques: In Vitro, Expressing, Concentration Assay

Journal: The Journal of Clinical Investigation

Article Title: Zika virus infects human testicular tissue and germ cells

doi: 10.1172/JCI121735

Figure Lengend Snippet: (A–C) Primary testicular cells were infected with ZIKV (MOI of 1, corresponding to 7.15 × 105 TCID50 U/ml per 0.5 million cells). ZIKV RNA detected by RT-qPCR in cells (A) and culture supernatants (B). (C) Viral titers determined by infectivity assay of tissue supernatants on VeroE6 cells. Each dot represents an independent donor. Bars represent median values. Dotted lines indicate detection limit. *P < 0.05 (Friedman-Dunn nonparametric comparison). (D) Immunofluorescence against ZIKV NS1 or ZIKV-E proteins combined with cell markers for all germ cells (DDX4) or specific germ cell types (STRA8, MAGEA-4), Sertoli cells (FSHR), and peritubular cells (α-SMA). Nuclei are stained in blue. (E) Detection of infected germ cells in semen from ZIKV-infected men. Immunofluorescence labeling of semen cell smears from 2 ZIKV-infected patients, one on day 7 (top row) and one on day 11 (middle row) after onset of symptoms. ZIKV-E or NS1 protein colabeled with the germ cell marker DDX4. Bottom panels show semen from a healthy individual stained with anti–ZIKV-E antibody and IgG isotype as a negative control. Nuclei are stained in blue. In the merge panels, brightfield images are included to visualize the cell’s morphology. Scale bars: 20μm.

Article Snippet: Infected cell characterization was performed using rabbit anti-DDX4 (5 μg/ml, Abcam, ab13840), anti-STRA8 (9.6 μg/ml, Thermo Fisher Scientific PA5-35047), and anti-FSHR (10 μg/ml, Origene, TA313897), detected using Alexa Fluor 488 (Invitrogen) or Alexa Fluor 647 goat anti-rabbit (Jackson ImmunoResearch Laboratories Inc.); mouse anti–MAGEA-4 (clone 57B, 4 μg/ml, provided by Giulio Spagnoli, Ludwig Institute for Cancer Research, Basel, Switzerland) coupled to Alexa Fluor 647 (Zenon labeling kit, Molecular Probes) and mouse anti–α-SMA (clone 1A4, 0.5 μg/ml, Dako, M0851) detected using Alexa Fluor 555 goat-anti mouse antibody.

Techniques: Infection, Quantitative RT-PCR, Immunofluorescence, Staining, Labeling, Marker, Negative Control

Journal: The EPMA Journal

Article Title: TMT-based quantitative proteomics revealed follicle-stimulating hormone (FSH)-related molecular characterizations for potentially prognostic assessment and personalized treatment of FSH-positive non-functional pituitary adenomas

doi: 10.1007/s13167-019-00187-w

Figure Lengend Snippet: Western blot of DEPs and relatively quantitative expressions between FSH + and FSH − NFPAs . a Western blot of ITGA1, ITGA6, ITGB4, AKT, pAKT, and FSHR. b The relative expression of proteins in FSH + and FSH − NFPAs. n = 3. * p < 0.05; ** p < 0.01

Article Snippet: The separated proteins were transferred to polyvinylidene difluoride (PVDF) membranes (0.45 μm; GE Healthcare), and incubated with primary antibodies against human ITGA1 (Cusabio), ITGA6 (RnD), ITGB4 (Cusabio), FSHR (Novus), AKT1/AKT2/AKT3 (Cusabio), phospho-AKT1/AKT2/AKT3 (S473) (Cusabio), and β-actin (Santa Cruz Biotechnology) (1:1000 dilution for each one) at 4 °C overnight, and followed by incubation for 2 h with horseradish peroxidase-conjugated secondary antibody (1:5000 dilution) at room temperature.

Techniques: Western Blot, Expressing

Journal: Scientific Reports

Article Title: Exosomes derived from human umbilical cord mesenchymal stem cells protect against cisplatin-induced ovarian granulosa cell stress and apoptosis in vitro

doi: 10.1038/s41598-017-02786-x

Figure Lengend Snippet: Typical characteristics of OGCs. ( a , b ) The OGC morphology was polygonal and fibre-like. ( c , d ) After FSHR immunostaining, a large number of adherent cells were dyed with DAB, producing a brown colour, and the dyed cells accounted for approximately 70–80% of adherent cells. Brown cells stained with DAB were OGCs. See arrows ( a and c , ×200 magnification; b and d , ×400 magnification); n = 5.

Article Snippet: The FSHR is a specific marker of OGCs, and thus, OGCs were identified using immunohistochemistry with an FSHR rabbit antimouse polyclonal antibody (Boster, Wuhan, China).

Techniques: Immunostaining, Staining