Journal: Oncotarget

Article Title: Combining BET and HDAC inhibitors synergistically induces apoptosis of melanoma and suppresses AKT and YAP signaling

doi:

Figure Lengend Snippet: A. Induction of BIM mRNA expression level in Patient-1-post and Me1007 cells was determined by qRT-PCR. Mean expression levels (± SEM) of n = 2 experiments are presented. B. Knockdown of BIM protein expression by siRNA was performed in Patient-1-post and Me1007 cells. After 24 h, transfected cells were treated with drugs as described before and incubated for a further 48 h. Expression of the BIM EL isoform is shown and α-Tubulin served as internal control. C. Knockdown of BIM reduces apoptosis in combination-treated in both cell lines. Mean (± SEM) of n = 3 experiments is shown. D. FOXO3a and BIM mRNA expression level analyzed by qRT-PCR are reduced upon siRNA-mediated knockdown of FOXO3a in Me1007 cells. E. Knockdown of FOXO3a reduces percentage of apoptosis in combination-treated Me1007 cells.

Article Snippet: RNA was extracted from cell lines using RNeasy Plus mini prep kit (Qiagen), quantitated using a Nanodrop (Thermo Scientific, Wilmington, DE, USA) and 1 μg RNA reverse transcribed with SuperScriptIII (Invitrogen). cDNA was amplified on AB7900 (Applied Biosystems, Mulgrave, VIC) using Universal PCR Master Mix and Taqman probes specific for BIM (Hs00708019_s1), YAP (Hs00371735_m1), Foxo3a (Hs00818121_m1) and normalized to levels of endogenous 18S (Hs99999901_s1) (Applied Biosystems).

Techniques: Expressing, Quantitative RT-PCR, Knockdown, Transfection, Incubation, Control

Journal: Scientific Reports

Article Title: Autophagy is a pro-survival adaptive response to heat shock in bovine cumulus-oocyte complexes

doi: 10.1038/s41598-020-69939-3

Figure Lengend Snippet: Genes evaluated by RT-qPCR using Applied BiosystemsTaqMan Assay 83 .

Article Snippet: Bt03649334_s1 , FSHR , Follicle-stimulating hormone receptor.

Techniques: RNA Binding Assay, Histone Deacetylase Assay, Binding Assay