Journal: Current protocols in chemical biology

Article Title: A Detailed Guide for Metabolic Incorporation of N -acetyl muramic Acid Probes into Bacterial Peptidoglycan

doi: 10.1002/cpch.74

Figure Lengend Snippet: Troubleshooting the Bacterial Cell Wall Labeling (Basic Protocol 4)

Article Snippet: Materials Stock solutions of sugars (0.1 M of NAM, 2-AzNAM, 2-AlkNAM, or 3-AzNAM in Milli-Q ® water) E. coli ΔMurQ-KU cells (see Liang, et. al 2017 2017 for strain construction; for access to strain, contact corresponding author) Petri Dishes with Clear Lid (Fisher- Catalog ID FB0875712) Isopropyl-1-thio-β-D-galactoside (IPTG) (Gold Biotechnology-Catalog ID I2481) Lysogeny broth (LB) Bacto Agar (Fisher) Chloramphenicol (34 mg/mL stock in Milli-Q ® water) (Gold Biotechnology-Catalog ID C-105) Kanamycin (50 mg/mL stock in Milli-Q® water) (Gold Biotechnology-Catalog ID K-120) Fosfomycin (10 mg/mL in Milli-Q® water) (Gold Biotechnology- Catalog ID F-470) 1 x Phosphate buffered saline (PBS) Copper sulfate (CuSO 4 ) (Fisher) Freshly prepared (+)− sodium (L) ascorbate (Fisher) Tris[(1-benzyl-1H-1,2,3-triazol-4-yl)methyl]amine (TBTA) (Sigma) Fluor 488-Alkyne (Sigma- Catalog ID61621) Poly-L-lysine (Sigma) Milli-Q ® water Cover glasses (Zeiss) 1.5 mL sterile Eppendorf tubes Prolong Diamond Antifade Mountant (Invitrogen) Sterile culture tubes 37 °C incubator Zeiss Elyra PS.1 microscope with Plan-Apochromat × 63/1.4 Oil differential interference contrast (DIC) M27 objective Plate shaker UV/Vis Spectrophotometer Tweezers Preparation of Stock Bacterial Plate 1.

Techniques: Labeling, Fluorescence, Concentration Assay, Imaging

Journal: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences

Article Title: A fast and sensitive LC-MS/MS method for the quantification of fosfomycin in human urine and plasma using one sample preparation method and HILIC chromatography.

doi: 10.1016/j.jchromb.2017.07.036

Figure Lengend Snippet: Fig. 1. (a) Chemical structure of fosfomycin and (b) the internal standard: fosfomycin- 13C3. Only one of the enantiomers from the racemic mixture is depicted. The three 13C atoms are marked as C*.

Article Snippet: Fosfomycin was purchased from Santa Cruz Biotechnology Inc. (Huissen, the Netherlands, purity> 98%) and racemic fosfomycin-13C3 benzylamine salt, which was used as the internal standard, was purchased from Toronto Research Chemicals (North York, Canada, purity 96%).

Techniques:

Journal: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences

Article Title: A fast and sensitive LC-MS/MS method for the quantification of fosfomycin in human urine and plasma using one sample preparation method and HILIC chromatography.

doi: 10.1016/j.jchromb.2017.07.036

Figure Lengend Snippet: Fig. 2. Chromatograms of fosfomycin in urine (a) and plasma (b) and the internal standard in urine (c) and in plasma (d) after injection of calibration standard 3.

Article Snippet: Fosfomycin was purchased from Santa Cruz Biotechnology Inc. (Huissen, the Netherlands, purity> 98%) and racemic fosfomycin-13C3 benzylamine salt, which was used as the internal standard, was purchased from Toronto Research Chemicals (North York, Canada, purity 96%).

Techniques: Clinical Proteomics, Injection