Journal: Autophagy

Article Title: Isorhynchophylline, a natural alkaloid, promotes the degradation of alpha-synuclein in neuronal cells via inducing autophagy.

doi: 10.4161/auto.8.1.18313

Figure Lengend Snippet: Figure 4. IsoRhy induced autophagy in L3 Drosophila larvae fat body. (A) Ninety six hours after egg laying, the L3 larvae were collected and fed on culture medium containing 0.2 mg/ml of IsoRhy for 6 h and fat bodies were isolated for LysoTracker staining. Images were captured using a fluorescence microscope. As positive control, L3 larvae were either fed with 5 mM of rapamycin for 24 h or starved for 3 h to induce autophagy. (B) Quantification of lysotracker-positive spots in each group. Data were presented as the mean ± SEM of one representative experiment from three independent experiments. (***p , 0.001, one-way ANOVA for multiple comparison and Tukey’s test as post hoc test).

Article Snippet: The instant Drosophila food (173212) was purchased from Carolina Biological Supply Company.

Techniques: Isolation, Staining, Fluorescence, Microscopy, Positive Control, Comparison