fmk Search Results


caspase inhibitor z vad ome fmk  (Santa Cruz Biotechnology)
93
Santa Cruz Biotechnology caspase inhibitor z vad ome fmk
Caspase Inhibitor Z Vad Ome Fmk, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/caspase inhibitor z vad ome fmk/product/Santa Cruz Biotechnology
Average 93 stars, based on 1 article reviews
caspase inhibitor z vad ome fmk - by Bioz Stars, 2026-05
93/100 stars
  Buy from Supplier
caspase 3 inhibitor z devd fmk  (Selleck Chemicals)
94
Selleck Chemicals caspase 3 inhibitor z devd fmk
A-B: Annexin V/PI staining combined with flow cytometry analysis to assess cell death in EMX2-stably expressed ACHN cells and control cells in both adhered and suspension conditions. Representative images (A) and statistics (B) were shown. C-D: Illustrative stacked histograms and quantified mean fluorescence intensity (MFI) of CMXRos staining were presented for EMX2 overexpressing ACHN cells and control cells in both adherent and suspension states. The results were expressed as fold change relative to the control. Representative images (C) and statistics (D) were shown. E-F: Confocal imaging displays the fluorescence intensity ratio of green fluorescent monomers (depolarization)/red fluorescent aggregates (polarization) in mitochondria of EMX2-overexpressing cells compared to control cells. Representative images (E) and statistics (F) were shown. G. Heat map showing the expression of mitochondrial ETC (electron-transport chain) regulated genes in EMX2-overexpressing ACHN cells compared to control cells under suspension condition. H. Annexin V/PI staining followed by flow cytometry analysis was conducted to detect the cell death of EMX2 overexpressing ACHN cells and control cells in suspension condition aided by an introduction of Matrigel (80 μg/ml). The results were expressed as fold change relative to the control. I-J: Western blot analyzed the protein level of <t>CASPASE-3,</t> cleaved CASPASE-3, PARP1, cleaved PARP1 and BCL-2 in EMX2 overexpressing and control cells in both adhered and suspension conditions. Representative images (I) and statistics (J) were shown. K. Cell death of EMX2 overexpressing ACHN cells and control cells under both adherent and suspension conditions, with or without Talazoparib (15 μM), was analyzed by Annexin V/PI staining followed by flow cytometry. The results were expressed as fold change relative to the control. L. Annexin V/PI staining followed by flow cytometry analysis was performed to check cell death of EMX2 overexpressing ACHN cells and control cells in both adhered and suspension conditions with or without Z-DEVD-FMK (10 μM). The results were expressed as fold change relative to the control. Error bars represent the mean ± SD of three biological replicates. Two-sided Student’s t test was used for statistical analysis. * P < 0.05, ** P < 0.01, *** P < 0.001, and ns: not significant.
Caspase 3 Inhibitor Z Devd Fmk, supplied by Selleck Chemicals, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/caspase 3 inhibitor z devd fmk/product/Selleck Chemicals
Average 94 stars, based on 1 article reviews
caspase 3 inhibitor z devd fmk - by Bioz Stars, 2026-05
94/100 stars
  Buy from Supplier
apoptosis inhibitor zvad fmk  (Selleck Chemicals)
96
Selleck Chemicals apoptosis inhibitor zvad fmk
A-B: Annexin V/PI staining combined with flow cytometry analysis to assess cell death in EMX2-stably expressed ACHN cells and control cells in both adhered and suspension conditions. Representative images (A) and statistics (B) were shown. C-D: Illustrative stacked histograms and quantified mean fluorescence intensity (MFI) of CMXRos staining were presented for EMX2 overexpressing ACHN cells and control cells in both adherent and suspension states. The results were expressed as fold change relative to the control. Representative images (C) and statistics (D) were shown. E-F: Confocal imaging displays the fluorescence intensity ratio of green fluorescent monomers (depolarization)/red fluorescent aggregates (polarization) in mitochondria of EMX2-overexpressing cells compared to control cells. Representative images (E) and statistics (F) were shown. G. Heat map showing the expression of mitochondrial ETC (electron-transport chain) regulated genes in EMX2-overexpressing ACHN cells compared to control cells under suspension condition. H. Annexin V/PI staining followed by flow cytometry analysis was conducted to detect the cell death of EMX2 overexpressing ACHN cells and control cells in suspension condition aided by an introduction of Matrigel (80 μg/ml). The results were expressed as fold change relative to the control. I-J: Western blot analyzed the protein level of <t>CASPASE-3,</t> cleaved CASPASE-3, PARP1, cleaved PARP1 and BCL-2 in EMX2 overexpressing and control cells in both adhered and suspension conditions. Representative images (I) and statistics (J) were shown. K. Cell death of EMX2 overexpressing ACHN cells and control cells under both adherent and suspension conditions, with or without Talazoparib (15 μM), was analyzed by Annexin V/PI staining followed by flow cytometry. The results were expressed as fold change relative to the control. L. Annexin V/PI staining followed by flow cytometry analysis was performed to check cell death of EMX2 overexpressing ACHN cells and control cells in both adhered and suspension conditions with or without Z-DEVD-FMK (10 μM). The results were expressed as fold change relative to the control. Error bars represent the mean ± SD of three biological replicates. Two-sided Student’s t test was used for statistical analysis. * P < 0.05, ** P < 0.01, *** P < 0.001, and ns: not significant.
Apoptosis Inhibitor Zvad Fmk, supplied by Selleck Chemicals, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/apoptosis inhibitor zvad fmk/product/Selleck Chemicals
Average 96 stars, based on 1 article reviews
apoptosis inhibitor zvad fmk - by Bioz Stars, 2026-05
96/100 stars
  Buy from Supplier
zvad  (Cell Signaling Technology Inc)
94
Cell Signaling Technology Inc zvad
A-B: Annexin V/PI staining combined with flow cytometry analysis to assess cell death in EMX2-stably expressed ACHN cells and control cells in both adhered and suspension conditions. Representative images (A) and statistics (B) were shown. C-D: Illustrative stacked histograms and quantified mean fluorescence intensity (MFI) of CMXRos staining were presented for EMX2 overexpressing ACHN cells and control cells in both adherent and suspension states. The results were expressed as fold change relative to the control. Representative images (C) and statistics (D) were shown. E-F: Confocal imaging displays the fluorescence intensity ratio of green fluorescent monomers (depolarization)/red fluorescent aggregates (polarization) in mitochondria of EMX2-overexpressing cells compared to control cells. Representative images (E) and statistics (F) were shown. G. Heat map showing the expression of mitochondrial ETC (electron-transport chain) regulated genes in EMX2-overexpressing ACHN cells compared to control cells under suspension condition. H. Annexin V/PI staining followed by flow cytometry analysis was conducted to detect the cell death of EMX2 overexpressing ACHN cells and control cells in suspension condition aided by an introduction of Matrigel (80 μg/ml). The results were expressed as fold change relative to the control. I-J: Western blot analyzed the protein level of <t>CASPASE-3,</t> cleaved CASPASE-3, PARP1, cleaved PARP1 and BCL-2 in EMX2 overexpressing and control cells in both adhered and suspension conditions. Representative images (I) and statistics (J) were shown. K. Cell death of EMX2 overexpressing ACHN cells and control cells under both adherent and suspension conditions, with or without Talazoparib (15 μM), was analyzed by Annexin V/PI staining followed by flow cytometry. The results were expressed as fold change relative to the control. L. Annexin V/PI staining followed by flow cytometry analysis was performed to check cell death of EMX2 overexpressing ACHN cells and control cells in both adhered and suspension conditions with or without Z-DEVD-FMK (10 μM). The results were expressed as fold change relative to the control. Error bars represent the mean ± SD of three biological replicates. Two-sided Student’s t test was used for statistical analysis. * P < 0.05, ** P < 0.01, *** P < 0.001, and ns: not significant.
Zvad, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/zvad/product/Cell Signaling Technology Inc
Average 94 stars, based on 1 article reviews
zvad - by Bioz Stars, 2026-05
94/100 stars
  Buy from Supplier
benzyloxycarbonyl phe ala fluoromethylketone  (Santa Cruz Biotechnology)
91
Santa Cruz Biotechnology benzyloxycarbonyl phe ala fluoromethylketone
A-B: Annexin V/PI staining combined with flow cytometry analysis to assess cell death in EMX2-stably expressed ACHN cells and control cells in both adhered and suspension conditions. Representative images (A) and statistics (B) were shown. C-D: Illustrative stacked histograms and quantified mean fluorescence intensity (MFI) of CMXRos staining were presented for EMX2 overexpressing ACHN cells and control cells in both adherent and suspension states. The results were expressed as fold change relative to the control. Representative images (C) and statistics (D) were shown. E-F: Confocal imaging displays the fluorescence intensity ratio of green fluorescent monomers (depolarization)/red fluorescent aggregates (polarization) in mitochondria of EMX2-overexpressing cells compared to control cells. Representative images (E) and statistics (F) were shown. G. Heat map showing the expression of mitochondrial ETC (electron-transport chain) regulated genes in EMX2-overexpressing ACHN cells compared to control cells under suspension condition. H. Annexin V/PI staining followed by flow cytometry analysis was conducted to detect the cell death of EMX2 overexpressing ACHN cells and control cells in suspension condition aided by an introduction of Matrigel (80 μg/ml). The results were expressed as fold change relative to the control. I-J: Western blot analyzed the protein level of <t>CASPASE-3,</t> cleaved CASPASE-3, PARP1, cleaved PARP1 and BCL-2 in EMX2 overexpressing and control cells in both adhered and suspension conditions. Representative images (I) and statistics (J) were shown. K. Cell death of EMX2 overexpressing ACHN cells and control cells under both adherent and suspension conditions, with or without Talazoparib (15 μM), was analyzed by Annexin V/PI staining followed by flow cytometry. The results were expressed as fold change relative to the control. L. Annexin V/PI staining followed by flow cytometry analysis was performed to check cell death of EMX2 overexpressing ACHN cells and control cells in both adhered and suspension conditions with or without Z-DEVD-FMK (10 μM). The results were expressed as fold change relative to the control. Error bars represent the mean ± SD of three biological replicates. Two-sided Student’s t test was used for statistical analysis. * P < 0.05, ** P < 0.01, *** P < 0.001, and ns: not significant.
Benzyloxycarbonyl Phe Ala Fluoromethylketone, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/benzyloxycarbonyl phe ala fluoromethylketone/product/Santa Cruz Biotechnology
Average 91 stars, based on 1 article reviews
benzyloxycarbonyl phe ala fluoromethylketone - by Bioz Stars, 2026-05
91/100 stars
  Buy from Supplier
cellpermeant pan caspase inhibitor carbobenzoxy valyl alanyl aspartyl  (Santa Cruz Biotechnology)
94
Santa Cruz Biotechnology cellpermeant pan caspase inhibitor carbobenzoxy valyl alanyl aspartyl
A-B: Annexin V/PI staining combined with flow cytometry analysis to assess cell death in EMX2-stably expressed ACHN cells and control cells in both adhered and suspension conditions. Representative images (A) and statistics (B) were shown. C-D: Illustrative stacked histograms and quantified mean fluorescence intensity (MFI) of CMXRos staining were presented for EMX2 overexpressing ACHN cells and control cells in both adherent and suspension states. The results were expressed as fold change relative to the control. Representative images (C) and statistics (D) were shown. E-F: Confocal imaging displays the fluorescence intensity ratio of green fluorescent monomers (depolarization)/red fluorescent aggregates (polarization) in mitochondria of EMX2-overexpressing cells compared to control cells. Representative images (E) and statistics (F) were shown. G. Heat map showing the expression of mitochondrial ETC (electron-transport chain) regulated genes in EMX2-overexpressing ACHN cells compared to control cells under suspension condition. H. Annexin V/PI staining followed by flow cytometry analysis was conducted to detect the cell death of EMX2 overexpressing ACHN cells and control cells in suspension condition aided by an introduction of Matrigel (80 μg/ml). The results were expressed as fold change relative to the control. I-J: Western blot analyzed the protein level of <t>CASPASE-3,</t> cleaved CASPASE-3, PARP1, cleaved PARP1 and BCL-2 in EMX2 overexpressing and control cells in both adhered and suspension conditions. Representative images (I) and statistics (J) were shown. K. Cell death of EMX2 overexpressing ACHN cells and control cells under both adherent and suspension conditions, with or without Talazoparib (15 μM), was analyzed by Annexin V/PI staining followed by flow cytometry. The results were expressed as fold change relative to the control. L. Annexin V/PI staining followed by flow cytometry analysis was performed to check cell death of EMX2 overexpressing ACHN cells and control cells in both adhered and suspension conditions with or without Z-DEVD-FMK (10 μM). The results were expressed as fold change relative to the control. Error bars represent the mean ± SD of three biological replicates. Two-sided Student’s t test was used for statistical analysis. * P < 0.05, ** P < 0.01, *** P < 0.001, and ns: not significant.
Cellpermeant Pan Caspase Inhibitor Carbobenzoxy Valyl Alanyl Aspartyl, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cellpermeant pan caspase inhibitor carbobenzoxy valyl alanyl aspartyl/product/Santa Cruz Biotechnology
Average 94 stars, based on 1 article reviews
cellpermeant pan caspase inhibitor carbobenzoxy valyl alanyl aspartyl - by Bioz Stars, 2026-05
94/100 stars
  Buy from Supplier
apoptosis inhibitor z vad fmk  (Beyotime)
99
Beyotime apoptosis inhibitor z vad fmk
A-B: Annexin V/PI staining combined with flow cytometry analysis to assess cell death in EMX2-stably expressed ACHN cells and control cells in both adhered and suspension conditions. Representative images (A) and statistics (B) were shown. C-D: Illustrative stacked histograms and quantified mean fluorescence intensity (MFI) of CMXRos staining were presented for EMX2 overexpressing ACHN cells and control cells in both adherent and suspension states. The results were expressed as fold change relative to the control. Representative images (C) and statistics (D) were shown. E-F: Confocal imaging displays the fluorescence intensity ratio of green fluorescent monomers (depolarization)/red fluorescent aggregates (polarization) in mitochondria of EMX2-overexpressing cells compared to control cells. Representative images (E) and statistics (F) were shown. G. Heat map showing the expression of mitochondrial ETC (electron-transport chain) regulated genes in EMX2-overexpressing ACHN cells compared to control cells under suspension condition. H. Annexin V/PI staining followed by flow cytometry analysis was conducted to detect the cell death of EMX2 overexpressing ACHN cells and control cells in suspension condition aided by an introduction of Matrigel (80 μg/ml). The results were expressed as fold change relative to the control. I-J: Western blot analyzed the protein level of <t>CASPASE-3,</t> cleaved CASPASE-3, PARP1, cleaved PARP1 and BCL-2 in EMX2 overexpressing and control cells in both adhered and suspension conditions. Representative images (I) and statistics (J) were shown. K. Cell death of EMX2 overexpressing ACHN cells and control cells under both adherent and suspension conditions, with or without Talazoparib (15 μM), was analyzed by Annexin V/PI staining followed by flow cytometry. The results were expressed as fold change relative to the control. L. Annexin V/PI staining followed by flow cytometry analysis was performed to check cell death of EMX2 overexpressing ACHN cells and control cells in both adhered and suspension conditions with or without Z-DEVD-FMK (10 μM). The results were expressed as fold change relative to the control. Error bars represent the mean ± SD of three biological replicates. Two-sided Student’s t test was used for statistical analysis. * P < 0.05, ** P < 0.01, *** P < 0.001, and ns: not significant.
Apoptosis Inhibitor Z Vad Fmk, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/apoptosis inhibitor z vad fmk/product/Beyotime
Average 99 stars, based on 1 article reviews
apoptosis inhibitor z vad fmk - by Bioz Stars, 2026-05
99/100 stars
  Buy from Supplier
pan caspase inhibitor  (R&D Systems)
96
R&D Systems pan caspase inhibitor
A-B: Annexin V/PI staining combined with flow cytometry analysis to assess cell death in EMX2-stably expressed ACHN cells and control cells in both adhered and suspension conditions. Representative images (A) and statistics (B) were shown. C-D: Illustrative stacked histograms and quantified mean fluorescence intensity (MFI) of CMXRos staining were presented for EMX2 overexpressing ACHN cells and control cells in both adherent and suspension states. The results were expressed as fold change relative to the control. Representative images (C) and statistics (D) were shown. E-F: Confocal imaging displays the fluorescence intensity ratio of green fluorescent monomers (depolarization)/red fluorescent aggregates (polarization) in mitochondria of EMX2-overexpressing cells compared to control cells. Representative images (E) and statistics (F) were shown. G. Heat map showing the expression of mitochondrial ETC (electron-transport chain) regulated genes in EMX2-overexpressing ACHN cells compared to control cells under suspension condition. H. Annexin V/PI staining followed by flow cytometry analysis was conducted to detect the cell death of EMX2 overexpressing ACHN cells and control cells in suspension condition aided by an introduction of Matrigel (80 μg/ml). The results were expressed as fold change relative to the control. I-J: Western blot analyzed the protein level of <t>CASPASE-3,</t> cleaved CASPASE-3, PARP1, cleaved PARP1 and BCL-2 in EMX2 overexpressing and control cells in both adhered and suspension conditions. Representative images (I) and statistics (J) were shown. K. Cell death of EMX2 overexpressing ACHN cells and control cells under both adherent and suspension conditions, with or without Talazoparib (15 μM), was analyzed by Annexin V/PI staining followed by flow cytometry. The results were expressed as fold change relative to the control. L. Annexin V/PI staining followed by flow cytometry analysis was performed to check cell death of EMX2 overexpressing ACHN cells and control cells in both adhered and suspension conditions with or without Z-DEVD-FMK (10 μM). The results were expressed as fold change relative to the control. Error bars represent the mean ± SD of three biological replicates. Two-sided Student’s t test was used for statistical analysis. * P < 0.05, ** P < 0.01, *** P < 0.001, and ns: not significant.
Pan Caspase Inhibitor, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pan caspase inhibitor/product/R&D Systems
Average 96 stars, based on 1 article reviews
pan caspase inhibitor - by Bioz Stars, 2026-05
96/100 stars
  Buy from Supplier
caspase 4 inhibitor  (R&D Systems)
93
R&D Systems caspase 4 inhibitor
A-B: Annexin V/PI staining combined with flow cytometry analysis to assess cell death in EMX2-stably expressed ACHN cells and control cells in both adhered and suspension conditions. Representative images (A) and statistics (B) were shown. C-D: Illustrative stacked histograms and quantified mean fluorescence intensity (MFI) of CMXRos staining were presented for EMX2 overexpressing ACHN cells and control cells in both adherent and suspension states. The results were expressed as fold change relative to the control. Representative images (C) and statistics (D) were shown. E-F: Confocal imaging displays the fluorescence intensity ratio of green fluorescent monomers (depolarization)/red fluorescent aggregates (polarization) in mitochondria of EMX2-overexpressing cells compared to control cells. Representative images (E) and statistics (F) were shown. G. Heat map showing the expression of mitochondrial ETC (electron-transport chain) regulated genes in EMX2-overexpressing ACHN cells compared to control cells under suspension condition. H. Annexin V/PI staining followed by flow cytometry analysis was conducted to detect the cell death of EMX2 overexpressing ACHN cells and control cells in suspension condition aided by an introduction of Matrigel (80 μg/ml). The results were expressed as fold change relative to the control. I-J: Western blot analyzed the protein level of <t>CASPASE-3,</t> cleaved CASPASE-3, PARP1, cleaved PARP1 and BCL-2 in EMX2 overexpressing and control cells in both adhered and suspension conditions. Representative images (I) and statistics (J) were shown. K. Cell death of EMX2 overexpressing ACHN cells and control cells under both adherent and suspension conditions, with or without Talazoparib (15 μM), was analyzed by Annexin V/PI staining followed by flow cytometry. The results were expressed as fold change relative to the control. L. Annexin V/PI staining followed by flow cytometry analysis was performed to check cell death of EMX2 overexpressing ACHN cells and control cells in both adhered and suspension conditions with or without Z-DEVD-FMK (10 μM). The results were expressed as fold change relative to the control. Error bars represent the mean ± SD of three biological replicates. Two-sided Student’s t test was used for statistical analysis. * P < 0.05, ** P < 0.01, *** P < 0.001, and ns: not significant.
Caspase 4 Inhibitor, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/caspase 4 inhibitor/product/R&D Systems
Average 93 stars, based on 1 article reviews
caspase 4 inhibitor - by Bioz Stars, 2026-05
93/100 stars
  Buy from Supplier
z devd fmk  (R&D Systems)
93
R&D Systems z devd fmk
A-B: Annexin V/PI staining combined with flow cytometry analysis to assess cell death in EMX2-stably expressed ACHN cells and control cells in both adhered and suspension conditions. Representative images (A) and statistics (B) were shown. C-D: Illustrative stacked histograms and quantified mean fluorescence intensity (MFI) of CMXRos staining were presented for EMX2 overexpressing ACHN cells and control cells in both adherent and suspension states. The results were expressed as fold change relative to the control. Representative images (C) and statistics (D) were shown. E-F: Confocal imaging displays the fluorescence intensity ratio of green fluorescent monomers (depolarization)/red fluorescent aggregates (polarization) in mitochondria of EMX2-overexpressing cells compared to control cells. Representative images (E) and statistics (F) were shown. G. Heat map showing the expression of mitochondrial ETC (electron-transport chain) regulated genes in EMX2-overexpressing ACHN cells compared to control cells under suspension condition. H. Annexin V/PI staining followed by flow cytometry analysis was conducted to detect the cell death of EMX2 overexpressing ACHN cells and control cells in suspension condition aided by an introduction of Matrigel (80 μg/ml). The results were expressed as fold change relative to the control. I-J: Western blot analyzed the protein level of <t>CASPASE-3,</t> cleaved CASPASE-3, PARP1, cleaved PARP1 and BCL-2 in EMX2 overexpressing and control cells in both adhered and suspension conditions. Representative images (I) and statistics (J) were shown. K. Cell death of EMX2 overexpressing ACHN cells and control cells under both adherent and suspension conditions, with or without Talazoparib (15 μM), was analyzed by Annexin V/PI staining followed by flow cytometry. The results were expressed as fold change relative to the control. L. Annexin V/PI staining followed by flow cytometry analysis was performed to check cell death of EMX2 overexpressing ACHN cells and control cells in both adhered and suspension conditions with or without Z-DEVD-FMK (10 μM). The results were expressed as fold change relative to the control. Error bars represent the mean ± SD of three biological replicates. Two-sided Student’s t test was used for statistical analysis. * P < 0.05, ** P < 0.01, *** P < 0.001, and ns: not significant.
Z Devd Fmk, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/z devd fmk/product/R&D Systems
Average 93 stars, based on 1 article reviews
z devd fmk - by Bioz Stars, 2026-05
93/100 stars
  Buy from Supplier
caspase 10  (R&D Systems)
92
R&D Systems caspase 10
A-B: Annexin V/PI staining combined with flow cytometry analysis to assess cell death in EMX2-stably expressed ACHN cells and control cells in both adhered and suspension conditions. Representative images (A) and statistics (B) were shown. C-D: Illustrative stacked histograms and quantified mean fluorescence intensity (MFI) of CMXRos staining were presented for EMX2 overexpressing ACHN cells and control cells in both adherent and suspension states. The results were expressed as fold change relative to the control. Representative images (C) and statistics (D) were shown. E-F: Confocal imaging displays the fluorescence intensity ratio of green fluorescent monomers (depolarization)/red fluorescent aggregates (polarization) in mitochondria of EMX2-overexpressing cells compared to control cells. Representative images (E) and statistics (F) were shown. G. Heat map showing the expression of mitochondrial ETC (electron-transport chain) regulated genes in EMX2-overexpressing ACHN cells compared to control cells under suspension condition. H. Annexin V/PI staining followed by flow cytometry analysis was conducted to detect the cell death of EMX2 overexpressing ACHN cells and control cells in suspension condition aided by an introduction of Matrigel (80 μg/ml). The results were expressed as fold change relative to the control. I-J: Western blot analyzed the protein level of <t>CASPASE-3,</t> cleaved CASPASE-3, PARP1, cleaved PARP1 and BCL-2 in EMX2 overexpressing and control cells in both adhered and suspension conditions. Representative images (I) and statistics (J) were shown. K. Cell death of EMX2 overexpressing ACHN cells and control cells under both adherent and suspension conditions, with or without Talazoparib (15 μM), was analyzed by Annexin V/PI staining followed by flow cytometry. The results were expressed as fold change relative to the control. L. Annexin V/PI staining followed by flow cytometry analysis was performed to check cell death of EMX2 overexpressing ACHN cells and control cells in both adhered and suspension conditions with or without Z-DEVD-FMK (10 μM). The results were expressed as fold change relative to the control. Error bars represent the mean ± SD of three biological replicates. Two-sided Student’s t test was used for statistical analysis. * P < 0.05, ** P < 0.01, *** P < 0.001, and ns: not significant.
Caspase 10, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/caspase 10/product/R&D Systems
Average 92 stars, based on 1 article reviews
caspase 10 - by Bioz Stars, 2026-05
92/100 stars
  Buy from Supplier
caspase 8 z i e ome t d ome fmk activity  (R&D Systems)
90
R&D Systems caspase 8 z i e ome t d ome fmk activity
A-B: Annexin V/PI staining combined with flow cytometry analysis to assess cell death in EMX2-stably expressed ACHN cells and control cells in both adhered and suspension conditions. Representative images (A) and statistics (B) were shown. C-D: Illustrative stacked histograms and quantified mean fluorescence intensity (MFI) of CMXRos staining were presented for EMX2 overexpressing ACHN cells and control cells in both adherent and suspension states. The results were expressed as fold change relative to the control. Representative images (C) and statistics (D) were shown. E-F: Confocal imaging displays the fluorescence intensity ratio of green fluorescent monomers (depolarization)/red fluorescent aggregates (polarization) in mitochondria of EMX2-overexpressing cells compared to control cells. Representative images (E) and statistics (F) were shown. G. Heat map showing the expression of mitochondrial ETC (electron-transport chain) regulated genes in EMX2-overexpressing ACHN cells compared to control cells under suspension condition. H. Annexin V/PI staining followed by flow cytometry analysis was conducted to detect the cell death of EMX2 overexpressing ACHN cells and control cells in suspension condition aided by an introduction of Matrigel (80 μg/ml). The results were expressed as fold change relative to the control. I-J: Western blot analyzed the protein level of <t>CASPASE-3,</t> cleaved CASPASE-3, PARP1, cleaved PARP1 and BCL-2 in EMX2 overexpressing and control cells in both adhered and suspension conditions. Representative images (I) and statistics (J) were shown. K. Cell death of EMX2 overexpressing ACHN cells and control cells under both adherent and suspension conditions, with or without Talazoparib (15 μM), was analyzed by Annexin V/PI staining followed by flow cytometry. The results were expressed as fold change relative to the control. L. Annexin V/PI staining followed by flow cytometry analysis was performed to check cell death of EMX2 overexpressing ACHN cells and control cells in both adhered and suspension conditions with or without Z-DEVD-FMK (10 μM). The results were expressed as fold change relative to the control. Error bars represent the mean ± SD of three biological replicates. Two-sided Student’s t test was used for statistical analysis. * P < 0.05, ** P < 0.01, *** P < 0.001, and ns: not significant.
Caspase 8 Z I E Ome T D Ome Fmk Activity, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/caspase 8 z i e ome t d ome fmk activity/product/R&D Systems
Average 90 stars, based on 1 article reviews
caspase 8 z i e ome t d ome fmk activity - by Bioz Stars, 2026-05
90/100 stars
  Buy from Supplier

Image Search Results


A-B: Annexin V/PI staining combined with flow cytometry analysis to assess cell death in EMX2-stably expressed ACHN cells and control cells in both adhered and suspension conditions. Representative images (A) and statistics (B) were shown. C-D: Illustrative stacked histograms and quantified mean fluorescence intensity (MFI) of CMXRos staining were presented for EMX2 overexpressing ACHN cells and control cells in both adherent and suspension states. The results were expressed as fold change relative to the control. Representative images (C) and statistics (D) were shown. E-F: Confocal imaging displays the fluorescence intensity ratio of green fluorescent monomers (depolarization)/red fluorescent aggregates (polarization) in mitochondria of EMX2-overexpressing cells compared to control cells. Representative images (E) and statistics (F) were shown. G. Heat map showing the expression of mitochondrial ETC (electron-transport chain) regulated genes in EMX2-overexpressing ACHN cells compared to control cells under suspension condition. H. Annexin V/PI staining followed by flow cytometry analysis was conducted to detect the cell death of EMX2 overexpressing ACHN cells and control cells in suspension condition aided by an introduction of Matrigel (80 μg/ml). The results were expressed as fold change relative to the control. I-J: Western blot analyzed the protein level of CASPASE-3, cleaved CASPASE-3, PARP1, cleaved PARP1 and BCL-2 in EMX2 overexpressing and control cells in both adhered and suspension conditions. Representative images (I) and statistics (J) were shown. K. Cell death of EMX2 overexpressing ACHN cells and control cells under both adherent and suspension conditions, with or without Talazoparib (15 μM), was analyzed by Annexin V/PI staining followed by flow cytometry. The results were expressed as fold change relative to the control. L. Annexin V/PI staining followed by flow cytometry analysis was performed to check cell death of EMX2 overexpressing ACHN cells and control cells in both adhered and suspension conditions with or without Z-DEVD-FMK (10 μM). The results were expressed as fold change relative to the control. Error bars represent the mean ± SD of three biological replicates. Two-sided Student’s t test was used for statistical analysis. * P < 0.05, ** P < 0.01, *** P < 0.001, and ns: not significant.

Journal: Molecular cancer research : MCR

Article Title: Empty spiracles homeobox 2 (EMX2) transcription factor functions as a tumor suppressor in renal cell carcinoma by targeting CADM1

doi: 10.1158/1541-7786.MCR-24-0496

Figure Lengend Snippet: A-B: Annexin V/PI staining combined with flow cytometry analysis to assess cell death in EMX2-stably expressed ACHN cells and control cells in both adhered and suspension conditions. Representative images (A) and statistics (B) were shown. C-D: Illustrative stacked histograms and quantified mean fluorescence intensity (MFI) of CMXRos staining were presented for EMX2 overexpressing ACHN cells and control cells in both adherent and suspension states. The results were expressed as fold change relative to the control. Representative images (C) and statistics (D) were shown. E-F: Confocal imaging displays the fluorescence intensity ratio of green fluorescent monomers (depolarization)/red fluorescent aggregates (polarization) in mitochondria of EMX2-overexpressing cells compared to control cells. Representative images (E) and statistics (F) were shown. G. Heat map showing the expression of mitochondrial ETC (electron-transport chain) regulated genes in EMX2-overexpressing ACHN cells compared to control cells under suspension condition. H. Annexin V/PI staining followed by flow cytometry analysis was conducted to detect the cell death of EMX2 overexpressing ACHN cells and control cells in suspension condition aided by an introduction of Matrigel (80 μg/ml). The results were expressed as fold change relative to the control. I-J: Western blot analyzed the protein level of CASPASE-3, cleaved CASPASE-3, PARP1, cleaved PARP1 and BCL-2 in EMX2 overexpressing and control cells in both adhered and suspension conditions. Representative images (I) and statistics (J) were shown. K. Cell death of EMX2 overexpressing ACHN cells and control cells under both adherent and suspension conditions, with or without Talazoparib (15 μM), was analyzed by Annexin V/PI staining followed by flow cytometry. The results were expressed as fold change relative to the control. L. Annexin V/PI staining followed by flow cytometry analysis was performed to check cell death of EMX2 overexpressing ACHN cells and control cells in both adhered and suspension conditions with or without Z-DEVD-FMK (10 μM). The results were expressed as fold change relative to the control. Error bars represent the mean ± SD of three biological replicates. Two-sided Student’s t test was used for statistical analysis. * P < 0.05, ** P < 0.01, *** P < 0.001, and ns: not significant.

Article Snippet: Cells were pretreated with the Caspase-3 inhibitor Z-DEVD-FMK (Selleck, Houston, TX) at 10 μM and PARP inhibitor Talazoparib (Selleck, Houston, TX) at 15 μM for 24 h.

Techniques: Staining, Flow Cytometry, Stable Transfection, Control, Suspension, Fluorescence, Imaging, Expressing, Western Blot

EMX2-mediated transcriptional regulation orchestrates multifaceted suppression of RCC progression. EMX2 directly binds to the CADM1 promoter and triggers transcriptional expression. Upregulation of EMX2-CADM1 enhances PARP1 activity and disrupts mitochondrial membrane potential (MMP), leading to parthanatos in movable and migrating RCC cells. EMX2-CADM1 also promotes RCC cell apoptosis under adherent conditions through the activation of the Caspase-3 pathway. Concurrently, increased EMX2-CADM1 expression inhibits PI3K-AKT signaling, impairing RCC cell proliferation and migration. These orchestrated effects ultimately converge to the suppression of renal tumor growth and invasion.

Journal: Molecular cancer research : MCR

Article Title: Empty spiracles homeobox 2 (EMX2) transcription factor functions as a tumor suppressor in renal cell carcinoma by targeting CADM1

doi: 10.1158/1541-7786.MCR-24-0496

Figure Lengend Snippet: EMX2-mediated transcriptional regulation orchestrates multifaceted suppression of RCC progression. EMX2 directly binds to the CADM1 promoter and triggers transcriptional expression. Upregulation of EMX2-CADM1 enhances PARP1 activity and disrupts mitochondrial membrane potential (MMP), leading to parthanatos in movable and migrating RCC cells. EMX2-CADM1 also promotes RCC cell apoptosis under adherent conditions through the activation of the Caspase-3 pathway. Concurrently, increased EMX2-CADM1 expression inhibits PI3K-AKT signaling, impairing RCC cell proliferation and migration. These orchestrated effects ultimately converge to the suppression of renal tumor growth and invasion.

Article Snippet: Cells were pretreated with the Caspase-3 inhibitor Z-DEVD-FMK (Selleck, Houston, TX) at 10 μM and PARP inhibitor Talazoparib (Selleck, Houston, TX) at 15 μM for 24 h.

Techniques: Expressing, Activity Assay, Membrane, Activation Assay, Migration